FastQCFastQC Report
Thu 26 May 2016
SRR936552_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936552_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441514
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36590.25383034781486685No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22540.15636337905840667No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18480.12819854680565018No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16810.11661350496769368No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14530.10079680114102256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG86650.033.3423231
TACATGG86950.032.677382
ACATGGG89150.031.2346823
TATTCCG1703.6270649E-927.9529085
CATGGGA45550.027.7494324
ATGGGAG20700.026.3999655
CATGGGG40350.025.9083634
GTATAGT1858.934876E-925.6855641
ATGGGGA22200.023.5459185
CCGTTGC2154.3941327E-822.1069039
ATGTGCG2257.125709E-821.1192427
GTGTAGC8600.020.9964541
GGACCGT2056.8064764E-720.861696
GTATAGA2851.9554136E-920.0077041
TACCTGG10500.019.4578462
GTCTATA2451.7496677E-719.3952221
GTATAGG3707.2759576E-1219.2641741
TACATCG1759.907031E-519.0079775
GTGTACG1759.909671E-519.0073171
AGTACAT57550.018.8237082
Produced by FastQC (version 0.11.5)