##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936552_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1441514 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.517503125186437 31.0 31.0 34.0 30.0 34.0 2 31.58572792217072 33.0 31.0 34.0 30.0 34.0 3 31.55222633980662 33.0 31.0 34.0 28.0 34.0 4 35.22095241530779 37.0 35.0 37.0 33.0 37.0 5 35.25884729527427 37.0 35.0 37.0 33.0 37.0 6 35.308061524203026 37.0 35.0 37.0 33.0 37.0 7 35.172064232466695 37.0 35.0 37.0 32.0 37.0 8 35.14659032100972 37.0 35.0 37.0 32.0 37.0 9 36.67105973303069 39.0 37.0 39.0 32.0 39.0 10-11 36.543836549627684 39.0 37.0 39.0 32.0 39.0 12-13 36.463534866813646 39.0 37.0 39.0 32.0 39.0 14-15 37.50751744346569 40.0 37.0 41.0 32.0 41.0 16-17 37.4196025845049 40.0 37.0 41.0 32.0 41.0 18-19 37.24954422919237 40.0 37.0 41.0 31.0 41.0 20-21 37.20981239169373 39.0 36.5 41.0 31.0 41.0 22-23 37.20016038692653 39.0 36.5 41.0 31.0 41.0 24-25 37.31766878434757 39.5 37.0 41.0 31.5 41.0 26-27 37.258638833892704 39.0 37.0 41.0 31.0 41.0 28-29 37.15974211835612 39.0 36.5 41.0 31.0 41.0 30-31 37.01070228939851 39.0 36.0 41.0 30.5 41.0 32-33 36.8988500978832 39.0 36.0 41.0 30.0 41.0 34-35 36.73163319953882 39.0 36.0 41.0 30.0 41.0 36-37 36.54705295959664 39.0 35.5 40.5 30.0 41.0 38-39 36.360033964290324 39.0 35.0 40.0 30.0 41.0 40-41 36.16118227086244 38.5 35.0 40.0 29.5 41.0 42-43 35.93370511836861 38.0 35.0 40.0 29.0 41.0 44-45 35.871023451732 38.0 35.0 40.0 28.5 41.0 46-47 35.86701030999352 38.0 35.0 40.0 28.0 41.0 48-49 35.71276484307471 38.0 35.0 40.0 28.0 41.0 50-51 34.732194415038634 37.5 34.0 39.5 27.0 40.5 52-53 34.84776041023535 38.0 33.5 39.5 27.0 40.5 54-55 35.295413017147254 38.0 34.0 40.0 28.0 41.0 56-57 35.1269512470916 38.0 34.0 40.0 27.0 41.0 58-59 34.8909316871012 38.0 34.0 40.0 26.5 41.0 60-61 34.57346824241735 37.0 34.0 40.0 26.0 41.0 62-63 34.22658364747065 37.0 33.0 40.0 26.0 41.0 64-65 33.84444445215239 36.0 33.0 39.0 26.0 41.0 66-67 33.460413148953116 36.0 33.0 39.0 25.0 40.5 68-69 33.00964055846839 35.0 32.0 38.5 23.5 40.0 70-71 32.59290509838961 35.0 32.0 37.5 23.0 40.0 72-73 32.18275334127868 35.0 31.5 37.0 22.5 39.0 74-75 31.77138272677199 35.0 31.0 36.5 21.5 39.0 76-77 31.319539733918646 34.5 31.0 36.0 20.0 38.5 78-79 30.876686594788534 34.0 30.5 35.5 19.5 37.0 80-81 30.446910331776174 34.0 30.0 35.0 18.0 37.0 82-83 30.086339432013844 34.0 30.0 35.0 17.0 36.5 84-85 29.74365909731019 34.0 29.5 35.0 13.0 36.0 86-87 29.41006538958345 34.0 29.0 35.0 7.5 36.0 88-89 29.078798402235428 34.0 29.0 35.0 6.0 35.0 90-91 28.743606721821642 34.0 29.0 35.0 2.0 35.0 92-93 28.334391826926414 34.0 29.0 35.0 2.0 35.0 94-95 27.866100155808407 33.0 27.0 35.0 2.0 35.0 96-97 27.27529077067583 33.0 26.5 35.0 2.0 35.0 98-99 26.528614012767132 33.0 25.0 35.0 2.0 35.0 100-101 23.932706515510777 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5280688219202503 1101 2 0.4215247423130819 1101 3 0.5533374502046726 1101 4 0.616484754483686 1101 5 0.4712750381455777 1101 6 0.5762869061410356 1101 7 0.49570939227307775 1101 8 0.5156102548160817 1101 9 0.6809990283040221 1101 10-11 0.6876978570859933 1101 12-13 0.7572857686680692 1101 14-15 0.8555858204610658 1101 16-17 1.0020489694493833 1101 18-19 1.2179026150640695 1101 20-21 1.1222575694083616 1101 22-23 0.947945002658237 1101 24-25 0.8000436207101203 1101 26-27 0.648095172702071 1101 28-29 0.7232748107862932 1101 30-31 0.4768984589696288 1101 32-33 0.446307985824447 1101 34-35 0.6209583642412753 1101 36-37 0.5840815560642554 1101 38-39 0.467299223616493 1101 40-41 0.5338520133418498 1101 42-43 0.5931621299113417 1101 44-45 0.5080657825545316 1101 46-47 0.7246042617059274 1101 48-49 0.6803107971085609 1101 50-51 0.5748689540155851 1101 52-53 0.7807997916894323 1101 54-55 0.6047676533082367 1101 56-57 0.7584753371543087 1101 58-59 0.6102032378151563 1101 60-61 0.5376420309386702 1101 62-63 0.6293397808380519 1101 64-65 0.4061047680815264 1101 66-67 0.35816274532664494 1101 68-69 0.3885217495316269 1101 70-71 0.2750999298307093 1101 72-73 0.2523113146935927 1101 74-75 0.14346263042337526 1101 76-77 0.27991419280948193 1101 78-79 0.21651707244119578 1101 80-81 0.3959323045589507 1101 82-83 0.26387598084151165 1101 84-85 0.21383855433839116 1101 86-87 0.21214240527386252 1101 88-89 0.3024200875933545 1101 90-91 0.16174266544952332 1101 92-93 0.1220767243078491 1101 94-95 0.5319869628019411 1101 96-97 0.11478488025727174 1101 98-99 0.3601147661413542 1101 100-101 0.7707825424342829 1102 1 0.3229395703364695 1102 2 0.33729927806132665 1102 3 0.43510790764014473 1102 4 0.3680789194033238 1102 5 0.41083495841227347 1102 6 0.3279132908771487 1102 7 0.36932215017480985 1102 8 0.3115857849394601 1102 9 0.38860490978091633 1102 10-11 0.3847785985140959 1102 12-13 0.26234785983439224 1102 14-15 0.505020792279467 1102 16-17 0.47403777700113636 1102 18-19 0.563598691979216 1102 20-21 0.42938097703343203 1102 22-23 0.29399943553217867 1102 24-25 0.5520775861246534 1102 26-27 0.4255879841030179 1102 28-29 0.2645691595736821 1102 30-31 0.20920870573046813 1102 32-33 0.32476409813843077 1102 34-35 0.079497904400057 1102 36-37 0.1242149174646201 1102 38-39 -0.13725282785501491 1102 40-41 0.03108576309841027 1102 42-43 -0.20418900737657708 1102 44-45 -0.06793091956824782 1102 46-47 -0.05413277160792518 1102 48-49 -0.03844672084861145 1102 50-51 -0.028984822433308466 1102 52-53 -0.0799137626861608 1102 54-55 0.16752523284106502 1102 56-57 0.14165236857793673 1102 58-59 -3.082267115388504E-4 1102 60-61 0.11182923236836473 1102 62-63 0.025598594747840764 1102 64-65 0.22927221231976347 1102 66-67 0.190045470922648 1102 68-69 0.19077334190655648 1102 70-71 -0.1566185821006414 1102 72-73 0.10114506473654927 1102 74-75 -0.24513395313048036 1102 76-77 -0.10766084356311367 1102 78-79 -0.3605743874458476 1102 80-81 -0.05185906282141772 1102 82-83 -0.04714190760059722 1102 84-85 -0.09107833285502309 1102 86-87 -0.31358851916279207 1102 88-89 -0.3006101145466147 1102 90-91 0.06480218762248313 1102 92-93 0.10428118267125441 1102 94-95 0.1008215577200886 1102 96-97 -0.1776983797580769 1102 98-99 -0.07145607163115386 1102 100-101 0.34377546266660985 1103 1 0.3109747452259306 1103 2 0.45514172432495315 1103 3 0.4568268149640531 1103 4 0.4737718559703268 1103 5 0.3625105264199817 1103 6 0.3786851356436003 1103 7 0.46232520362663365 1103 8 0.28356844921297863 1103 9 0.40971093788581214 1103 10-11 0.43204143614487833 1103 12-13 0.30629458311942415 1103 14-15 0.6202824424567623 1103 16-17 0.6095169172191461 1103 18-19 0.7521507101029314 1103 20-21 0.6879017766219278 1103 22-23 0.5567029989870704 1103 24-25 0.44165330406887904 1103 26-27 0.3581056043805617 1103 28-29 0.4552840394055693 1103 30-31 0.30076985137683465 1103 32-33 0.4671463377742029 1103 34-35 0.4818312231410289 1103 36-37 0.4654395267541247 1103 38-39 0.25135432203772723 1103 40-41 0.20255377299202593 1103 42-43 0.24932861837310583 1103 44-45 0.39124489468557044 1103 46-47 0.6054778038418789 1103 48-49 0.5999627481353187 1103 50-51 0.5585036860813446 1103 52-53 0.5428173529728966 1103 54-55 0.5688715150719403 1103 56-57 0.5156058225901106 1103 58-59 0.3752677155902546 1103 60-61 0.4794746817401645 1103 62-63 0.3414796907072173 1103 64-65 0.3442479433971215 1103 66-67 0.42487671025197926 1103 68-69 0.5488056165640032 1103 70-71 0.5644071424101469 1103 72-73 0.6048421765432295 1103 74-75 0.48861859141269903 1103 76-77 0.4431889791396877 1103 78-79 0.41657356269204726 1103 80-81 0.4901901973458074 1103 82-83 0.40546118743946025 1103 84-85 0.5689488089389663 1103 86-87 0.46039927250204826 1103 88-89 0.24452885034416383 1103 90-91 0.4876026626038765 1103 92-93 0.6316480182445154 1103 94-95 0.656587410497746 1103 96-97 0.7512144710058344 1103 98-99 1.0917284925679418 1103 100-101 1.5881719955213107 1104 1 0.36074033197746047 1104 2 0.3373750094097794 1104 3 0.29473444390464465 1104 4 0.3006293348438618 1104 5 0.24571898126150415 1104 6 0.2869047656910624 1104 7 0.2995357125774376 1104 8 0.3874685960871176 1104 9 0.4507478905667952 1104 10-11 0.5920012054034842 1104 12-13 0.540703840447172 1104 14-15 0.6188423043099291 1104 16-17 0.6618893868332307 1104 18-19 0.5424047331770723 1104 20-21 0.6718267750254654 1104 22-23 0.49459014005010005 1104 24-25 0.5525725445805989 1104 26-27 0.03518206407532176 1104 28-29 0.09160957847622342 1104 30-31 0.16462457902110117 1104 32-33 0.2682522840480672 1104 34-35 0.27842251925206085 1104 36-37 0.37661127370488856 1104 38-39 0.27153687700909757 1104 40-41 0.24068849808195836 1104 42-43 0.4012641906500747 1104 44-45 0.37247121389202675 1104 46-47 0.5606299728961375 1104 48-49 0.6096459310468347 1104 50-51 0.4486906579198191 1104 52-53 0.27735698770331396 1104 54-55 0.39070281203441226 1104 56-57 0.4112992441188794 1104 58-59 0.3243899297710584 1104 60-61 0.19110913543224228 1104 62-63 0.25798294215999107 1104 64-65 0.2603680449318517 1104 66-67 0.31170247282674524 1104 68-69 -0.009097914801081686 1104 70-71 0.104733006093916 1104 72-73 0.22942044555704655 1104 74-75 0.14185594214388075 1104 76-77 0.16027666725565481 1104 78-79 0.09037063159517444 1104 80-81 0.2823001028354959 1104 82-83 0.15223168596007497 1104 84-85 0.03585822591250576 1104 86-87 0.05086589056544 1104 88-89 0.04685342621849031 1104 90-91 0.08117638703394192 1104 92-93 0.15676030245660755 1104 94-95 0.08375766209952573 1104 96-97 -0.1385560913731041 1104 98-99 -0.14481270136977287 1104 100-101 0.5191584469144885 1105 1 0.2824987193432733 1105 2 0.25655867052522296 1105 3 0.21504068028442092 1105 4 0.4082953333910382 1105 5 0.5227159410936224 1105 6 0.3296890751962991 1105 7 0.5100434609699178 1105 8 0.4621363299513561 1105 9 0.46745872486419415 1105 10-11 0.5177767172547547 1105 12-13 0.5508873939460557 1105 14-15 0.8654501966395642 1105 16-17 0.688859610675685 1105 18-19 0.6371548946019132 1105 20-21 0.7223786462084405 1105 22-23 0.4116493829675889 1105 24-25 0.4157905488314526 1105 26-27 0.3851699572120779 1105 28-29 0.5304810170406142 1105 30-31 0.4176117793762302 1105 32-33 0.36310098188766915 1105 34-35 0.27780338771771085 1105 36-37 0.2249677670192085 1105 38-39 0.06354584281393727 1105 40-41 0.21793112634745881 1105 42-43 0.14312518368191007 1105 44-45 0.19156995881386507 1105 46-47 0.3968730450799498 1105 48-49 0.4213157701600707 1105 50-51 0.47012434593940355 1105 52-53 0.5332365513181543 1105 54-55 0.5289682348329805 1105 56-57 0.3448522385497199 1105 58-59 0.5031676074056506 1105 60-61 0.5832659058592071 1105 62-63 0.5665016338116402 1105 64-65 0.5725161051433147 1105 66-67 0.5612884853640736 1105 68-69 0.5021836639338844 1105 70-71 0.5697520744035316 1105 72-73 0.46645433132206193 1105 74-75 0.3543613229719931 1105 76-77 0.605786702500076 1105 78-79 0.6823817833614321 1105 80-81 0.5639334868382946 1105 82-83 0.42934719216147954 1105 84-85 0.6871156581813302 1105 86-87 0.5691763441692466 1105 88-89 0.6416160308083363 1105 90-91 0.473624394782334 1105 92-93 0.6441664742664521 1105 94-95 0.8070796844837709 1105 96-97 0.8209193787767539 1105 98-99 0.8175357946887694 1105 100-101 1.1485629910238266 1106 1 0.03236833521223659 1106 2 0.007027721541835064 1106 3 0.04887592993553724 1106 4 0.17115795061013017 1106 5 0.15467254143001696 1106 6 0.11671144397816136 1106 7 0.23691481820497273 1106 8 0.30459038111643366 1106 9 0.3142499478201799 1106 10-11 0.38815690096276256 1106 12-13 0.27691880839991256 1106 14-15 0.1590115086200541 1106 16-17 0.16445463564340912 1106 18-19 0.25656894325187096 1106 20-21 0.03562560405759996 1106 22-23 -0.21675986076952114 1106 24-25 -0.3967160227732762 1106 26-27 -0.3863690995159388 1106 28-29 -0.1946959578030203 1106 30-31 -0.10870957165837325 1106 32-33 -0.0709208052744259 1106 34-35 0.0315934352079239 1106 36-37 -0.38242404384433115 1106 38-39 -0.11125517510798488 1106 40-41 0.02170763764188166 1106 42-43 0.019462516160565713 1106 44-45 -0.0977855261399938 1106 46-47 -0.17215299506753468 1106 48-49 -0.09940997952684683 1106 50-51 -0.28841035650007285 1106 52-53 -0.2404366831689302 1106 54-55 -0.28862759706667873 1106 56-57 -0.23659845738540497 1106 58-59 -0.18145090766493865 1106 60-61 -0.13663824040855843 1106 62-63 -0.17876078499301684 1106 64-65 -0.438944598843662 1106 66-67 -0.3370525883444415 1106 68-69 -0.1061446770175607 1106 70-71 -0.5531412132153015 1106 72-73 -0.39372549922223143 1106 74-75 -0.34114815323836467 1106 76-77 -0.5832267970169873 1106 78-79 -0.3926758109683881 1106 80-81 -0.21126261334003615 1106 82-83 -0.22479636836452954 1106 84-85 -0.09031417629282501 1106 86-87 -0.044806808070159576 1106 88-89 -0.1491260845759399 1106 90-91 0.19535531616934776 1106 92-93 0.2836488501395351 1106 94-95 0.3821138742944221 1106 96-97 0.43381404621425546 1106 98-99 0.27587837047837027 1106 100-101 0.7553454110860436 1107 1 0.21375230076370855 1107 2 0.20082671245733863 1107 3 0.25225711605480683 1107 4 0.3401118298348962 1107 5 0.315069397796357 1107 6 0.2875058413000531 1107 7 0.14984020482673088 1107 8 0.3442359692812573 1107 9 0.44215620017345714 1107 10-11 0.3997861890584389 1107 12-13 0.3548277991100406 1107 14-15 0.33550699375420123 1107 16-17 0.4988100606706283 1107 18-19 0.6062670394092748 1107 20-21 0.44708117764067623 1107 22-23 0.3608639985337021 1107 24-25 0.29570762841483145 1107 26-27 0.2871039242461535 1107 28-29 0.42189403487187604 1107 30-31 0.3021779640258444 1107 32-33 0.3655388758439173 1107 34-35 0.11758074976982869 1107 36-37 0.2911675849987816 1107 38-39 0.351503000598683 1107 40-41 0.3537638697997707 1107 42-43 0.2708116703505965 1107 44-45 0.12084659615502602 1107 46-47 0.24359752008584223 1107 48-49 0.2914627348009162 1107 50-51 0.13539383729343513 1107 52-53 0.31996085778661865 1107 54-55 0.3138824492800083 1107 56-57 0.48875034081823543 1107 58-59 0.40998915997247565 1107 60-61 0.3523898482868191 1107 62-63 0.21458620589510247 1107 64-65 0.35492079722542513 1107 66-67 0.30522113780617843 1107 68-69 0.39645676198896496 1107 70-71 0.4026615360949748 1107 72-73 0.4120327517892477 1107 74-75 0.28482362114841564 1107 76-77 0.40685607250715705 1107 78-79 0.3877699250677047 1107 80-81 0.21159924340282643 1107 82-83 0.16580268142564591 1107 84-85 0.23862238282487525 1107 86-87 0.40972055153770626 1107 88-89 0.4483238206084508 1107 90-91 0.35728890416987014 1107 92-93 0.6332208107345814 1107 94-95 0.5374425455752174 1107 96-97 0.8359225809879653 1107 98-99 1.0371754616588085 1107 100-101 1.4759376834430107 1108 1 -0.009342633250767562 1108 2 0.029711344215531454 1108 3 0.24953255241070238 1108 4 0.3448798162120781 1108 5 0.20606556637873297 1108 6 0.3081529251655226 1108 7 0.36955071993953936 1108 8 0.33995755293387475 1108 9 -0.13717543030760027 1108 10-11 0.1462740562359599 1108 12-13 0.10943614308619942 1108 14-15 0.5616777046950219 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0.2708479433251547 2311 2 0.35536406595919345 2311 3 0.5321856012724524 2311 4 0.4321718842041804 2311 5 0.48873921156464206 2311 6 0.4937882006110712 2311 7 0.480378934480548 2311 8 0.3509232782273699 2311 9 0.571597392236896 2311 10-11 0.5986883694697056 2311 12-13 0.6502165620396667 2311 14-15 0.8184266355034922 2311 16-17 0.8343452371451932 2311 18-19 0.8711091767311956 2311 20-21 1.0259555530143274 2311 22-23 0.987591238446619 2311 24-25 0.8879278578486876 2311 26-27 0.7903161674866581 2311 28-29 0.8570270816948806 2311 30-31 1.060747047982396 2311 32-33 0.7761118400978049 2311 34-35 0.6723400359755942 2311 36-37 0.9204745458461261 2311 38-39 0.9320532921849747 2311 40-41 1.1756937537292558 2311 42-43 1.0642047309351241 2311 44-45 1.090833511431434 2311 46-47 1.153014855088614 2311 48-49 0.9237769260860489 2311 50-51 1.04720900429286 2311 52-53 1.1053211952822508 2311 54-55 1.058296625901221 2311 56-57 1.1089338089467802 2311 58-59 1.0076403956892648 2311 60-61 1.1924877581150284 2311 62-63 1.0725112056131039 2311 64-65 1.2578648600321003 2311 66-67 1.3783844825322475 2311 68-69 1.1555794087127111 2311 70-71 0.9984622986185201 2311 72-73 1.0858788145282432 2311 74-75 1.0795956536797426 2311 76-77 1.162342350467295 2311 78-79 1.296751572614884 2311 80-81 1.3103584506490584 2311 82-83 1.402675940085814 2311 84-85 1.3501329916029192 2311 86-87 1.5082149623303458 2311 88-89 1.6028893739456223 2311 90-91 1.5744149461537873 2311 92-93 1.6541673664066927 2311 94-95 1.4064957643044202 2311 96-97 1.4864059261652294 2311 98-99 1.610969354702469 2311 100-101 1.371834325406624 2312 1 0.3596248190500191 2312 2 0.33941953725705787 2312 3 0.43247306868455127 2312 4 0.5252389632378964 2312 5 0.4090958694090361 2312 6 0.5814974818434138 2312 7 0.5271825942380843 2312 8 0.4982067111830304 2312 9 0.4401534136879164 2312 10-11 0.4179070528689621 2312 12-13 0.2639535392702612 2312 14-15 0.42929835564901 2312 16-17 0.3693255604964705 2312 18-19 0.37821061307698045 2312 20-21 0.42151517276433026 2312 22-23 0.6265728107942365 2312 24-25 0.37921511709473066 2312 26-27 0.4145769301674349 2312 28-29 0.10271953121461763 2312 30-31 0.36829604135081695 2312 32-33 0.38343333770389876 2312 34-35 0.4983801228105733 2312 36-37 0.5239087901537829 2312 38-39 0.47364685291495334 2312 40-41 0.696083619016072 2312 42-43 0.40467617749096263 2312 44-45 0.026907202215632253 2312 46-47 0.48540351102977297 2312 48-49 0.45140700509001874 2312 50-51 0.5724540950433479 2312 52-53 0.5118452186365019 2312 54-55 0.07675252939147725 2312 56-57 0.38320349303000967 2312 58-59 0.562714691146283 2312 60-61 0.6744030840931572 2312 62-63 0.5517248335509706 2312 64-65 0.610476352780978 2312 66-67 0.8002751259636156 2312 68-69 0.7993651562766715 2312 70-71 0.5583477081604542 2312 72-73 0.30089551737615494 2312 74-75 0.3284408276966566 2312 76-77 0.4227303323469336 2312 78-79 0.48076888013562424 2312 80-81 0.37429516816771624 2312 82-83 0.4554143150027983 2312 84-85 0.554662090243891 2312 86-87 0.3450479635892556 2312 88-89 0.5898076241612209 2312 90-91 0.5229890086630782 2312 92-93 0.7653785954785697 2312 94-95 0.7894192087587761 2312 96-97 0.6114537547949972 2312 98-99 0.983013148875802 2312 100-101 0.5795339913496669 2313 1 0.1742088216198887 2313 2 0.10337534979944252 2313 3 0.21141799497930336 2313 4 0.26178641656979806 2313 5 0.2072630536186182 2313 6 0.16412945528811207 2313 7 0.3578943049851304 2313 8 0.2873008326093043 2313 9 0.3065077699715957 2313 10-11 0.3879538101948654 2313 12-13 0.2863577938299926 2313 14-15 0.43772359746647993 2313 16-17 0.3447708258710591 2313 18-19 0.11830858930570542 2313 20-21 0.33147965861220996 2313 22-23 0.26881859014249443 2313 24-25 0.31796302015358435 2313 26-27 0.2097735964771772 2313 28-29 0.2358922477795602 2313 30-31 0.3633543987766714 2313 32-33 0.19637637229003957 2313 34-35 0.05245443483541834 2313 36-37 0.16961586086085134 2313 38-39 0.30031905193696673 2313 40-41 0.5215413768059918 2313 42-43 0.5204603442468638 2313 44-45 0.6829785160607926 2313 46-47 0.4869730937830923 2313 48-49 0.5776401849451105 2313 50-51 0.5389030367573113 2313 52-53 0.6324826887439343 2313 54-55 0.5924546031324169 2313 56-57 0.627315996153115 2313 58-59 0.4681417532871279 2313 60-61 0.4902711284947756 2313 62-63 0.7565879523716816 2313 64-65 0.7469702671900933 2313 66-67 0.7207475177257621 2313 68-69 0.5936106143913875 2313 70-71 0.6484903001581728 2313 72-73 1.0443923228870986 2313 74-75 0.913813101673167 2313 76-77 0.9737654181876714 2313 78-79 0.8139924586772267 2313 80-81 0.7151774896154102 2313 82-83 0.6631596125007455 2313 84-85 0.6077432552508526 2313 86-87 0.7467915478416707 2313 88-89 0.8944226793063095 2313 90-91 1.0367190654143386 2313 92-93 1.1000611096663881 2313 94-95 1.2303916540598472 2313 96-97 1.3806242584178001 2313 98-99 1.2724918582308398 2313 100-101 1.3352082248507564 2314 1 -0.5916229527859862 2314 2 -0.40055372477502615 2314 3 -0.43187630742952976 2314 4 -0.3620010842962671 2314 5 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2314 66-67 -0.6642599304832046 2314 68-69 -0.5046306844838782 2314 70-71 -0.5562690476327674 2314 72-73 -0.5860177327723903 2314 74-75 -0.5066007646978754 2314 76-77 -0.5147548132264497 2314 78-79 -0.46541353222325554 2314 80-81 0.06617722402036108 2314 82-83 -0.23889643960207607 2314 84-85 -0.42431146887672355 2314 86-87 -0.3090815670108604 2314 88-89 -0.27781715302238297 2314 90-91 -0.32311170434940095 2314 92-93 -0.03983530826474535 2314 94-95 -0.3071568459055136 2314 96-97 -0.4207654965419003 2314 98-99 -0.29454923971599456 2314 100-101 -0.9584042142355962 2315 1 -0.9320444872534317 2315 2 -1.389551356347983 2315 3 -0.9758857811377126 2315 4 -0.9178099932256956 2315 5 -1.0523742323049703 2315 6 -0.6444237513948892 2315 7 -0.4087023879883631 2315 8 -0.2540739350000578 2315 9 -0.5775462308410013 2315 10-11 -0.9360172071528581 2315 12-13 -0.7939847284240997 2315 14-15 -0.8458899384417222 2315 16-17 -1.0514782968927534 2315 18-19 -1.0656601503059733 2315 20-21 -1.1945003834951322 2315 22-23 -1.2448170459614758 2315 24-25 -1.4855791672117462 2315 26-27 -1.3835797234770197 2315 28-29 -1.2650557829021665 2315 30-31 -1.087038664593642 2315 32-33 -1.0858228126498162 2315 34-35 -0.9313659441306115 2315 36-37 -0.9314294353063914 2315 38-39 -1.2637268844587908 2315 40-41 -1.4566800806988454 2315 42-43 -1.3967501062523908 2315 44-45 -1.5841475966440512 2315 46-47 -2.004120650445614 2315 48-49 -2.6884163152211045 2315 50-51 -2.3438474218679275 2315 52-53 -2.1507968818104786 2315 54-55 -2.312648449033894 2315 56-57 -2.240729213829532 2315 58-59 -2.4513448942547598 2315 60-61 -2.753021862234224 2315 62-63 -2.9036353888824245 2315 64-65 -2.1906366940637767 2315 66-67 -2.113690312392066 2315 68-69 -2.4380129804917097 2315 70-71 -2.1953970565599192 2315 72-73 -2.0418630790409793 2315 74-75 -1.9336663063616975 2315 76-77 -1.9022872652316387 2315 78-79 -1.8593324819169545 2315 80-81 -2.046449737333287 2315 82-83 -1.8003673694374243 2315 84-85 -1.8220378358462774 2315 86-87 -2.069204315297167 2315 88-89 -2.2595799801044336 2315 90-91 -2.1179253615195854 2315 92-93 -2.005564282781382 2315 94-95 -2.28353947212673 2315 96-97 -2.2397402409111926 2315 98-99 -2.52046501252919 2315 100-101 -2.824844286199344 2316 1 -5.042362264904277 2316 2 -5.184425470194505 2316 3 -6.438465828483853 2316 4 -8.19438349081079 2316 5 -8.888469952746952 2316 6 -9.515091357368377 2316 7 -12.049220783804639 2316 8 -11.515355136805049 2316 9 -11.712140012376004 2316 10-11 -11.126661584322626 2316 12-13 -9.705221820888703 2316 14-15 -9.863448787917807 2316 16-17 -8.68389517141279 2316 18-19 -9.030731264717353 2316 20-21 -10.339339273336023 2316 22-23 -10.143736843121971 2316 24-25 -10.071389287608035 2316 26-27 -7.813349878906912 2316 28-29 -6.083950447258644 2316 30-31 -8.602336513707968 2316 32-33 -6.24995795224477 2316 34-35 -4.356272965449065 2316 36-37 -5.0988267620899705 2316 38-39 -4.438134899030739 2316 40-41 -3.721460676397072 2316 42-43 -3.946151774156661 2316 44-45 -4.274872893826764 2316 46-47 -3.8704169890530267 2316 48-49 -4.617503410541708 2316 50-51 -4.916327160131349 2316 52-53 -4.828186243526112 2316 54-55 -4.033058697632871 2316 56-57 -4.930656417551216 2316 58-59 -5.161047521140521 2316 60-61 -5.379480009134195 2316 62-63 -4.973271026690792 2316 64-65 -5.756463537929406 2316 66-67 -5.3211025855780285 2316 68-69 -5.374835870383372 2316 70-71 -5.031518163672629 2316 72-73 -4.0108945140860115 2316 74-75 -4.204594443043533 2316 76-77 -4.814651684543282 2316 78-79 -5.016937693800454 2316 80-81 -5.705767756157503 2316 82-83 -5.031205986371603 2316 84-85 -5.008990832425422 2316 86-87 -6.14244168701515 2316 88-89 -7.7469120314108935 2316 90-91 -6.4319124650908535 2316 92-93 -6.004972456020024 2316 94-95 -5.447673025054769 2316 96-97 -6.827079114885411 2316 98-99 -8.495228281824645 2316 100-101 -9.14816099126335 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2597.0 3 1038.0 4 1192.0 5 1319.0 6 1421.0 7 1530.0 8 1998.0 9 2439.0 10 2963.0 11 3558.0 12 4132.0 13 4741.0 14 5225.0 15 5949.0 16 6909.0 17 8050.0 18 7237.0 19 7001.0 20 7902.0 21 8856.0 22 10069.0 23 11674.0 24 13697.0 25 16050.0 26 18901.0 27 22681.0 28 27301.0 29 33405.0 30 41095.0 31 51436.0 32 65216.0 33 84596.0 34 113234.0 35 155350.0 36 219201.0 37 272249.0 38 181198.0 39 18102.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.2213116688906 16.915788319029375 12.680627661598146 24.18227235048188 2 16.301529088063955 18.78422405806857 39.40821134516032 25.506035508707164 3 20.561406478855844 23.110403095041676 28.09127764136288 28.236912784739605 4 12.966988741825247 14.537720956858118 35.64355473832116 36.85173556299547 5 14.56219055648656 37.28394033703538 33.18497392607704 14.968895180401015 6 34.57973369937467 33.26245677489915 17.404613314476098 14.75319621125008 7 31.78814476006393 30.964243214458786 19.166559053335945 18.08105297214134 8 28.49858502451305 32.84832448438599 19.04250895942904 19.61058153167192 9 27.96815968476255 15.747085261566273 19.029975604642477 37.254779449028696 10-11 26.456495594633385 24.861608839541223 26.527613153798228 22.15428241202716 12-13 27.514577714730677 21.952247834336642 26.736972360291983 23.796202090640698 14-15 24.1222055349355 23.660128088034845 24.67190280411192 27.545763572917735 16-17 24.025105502804152 26.93680937309123 25.25591370981176 23.782171414292854 18-19 23.875183714122606 25.94430360418044 26.798541739328098 23.381970942368856 20-21 25.007346193942993 25.0133480430676 26.053541351179295 23.925764411810114 22-23 24.39042158712582 24.8639512774374 26.266258286335948 24.479368849100833 24-25 24.34097086192983 25.103682902746584 26.479367848273593 24.075978387049993 26-27 24.054037831894714 25.24580392516056 26.638903894386278 24.06125434855845 28-29 23.74717640333448 25.659902984826115 26.512878930835065 24.080041681004342 30-31 23.911489390041414 25.242499216961228 26.42765398114949 24.41835741184787 32-33 23.777597777087838 25.241188397702853 26.38376108550823 24.59745273970108 34-35 24.422494794734583 25.209164597395876 26.2474077743527 24.120932833516846 36-37 24.442173571899826 25.050418226735292 26.274469403229954 24.232938798134924 38-39 24.20867501977272 25.059248775479055 26.51413922768458 24.217936977063648 40-41 24.142601892066924 25.2470186920783 26.46982408904432 24.140555326810457 42-43 23.891484989711948 25.19063766495222 26.242335991253423 24.675541354082405 44-45 23.993449914950176 25.111723278677605 26.205439026352956 24.689387780019256 46-47 24.433032779919632 25.361435857947324 25.950784161719586 24.25474720041346 48-49 24.355098407976698 25.293635306025188 25.84927218125021 24.501994104747904 50-51 23.994582042001316 25.503875296782354 26.324963016019744 24.17657964519659 52-53 24.40637025125684 25.332307096779566 25.956304244221172 24.30501840774242 54-55 23.84325330963597 25.2002326726028 26.165643607868272 24.790870409892957 56-57 24.19741823813744 25.48517732919632 25.752337477865282 24.565066954800958 58-59 24.51104279305607 24.9291257027161 25.814928678239884 24.744902825987946 60-61 24.144491835851216 25.155124377004963 26.512982994594235 24.18740079254959 62-63 23.86681609636759 25.279183450625936 26.474031386480775 24.3799690665257 64-65 23.79381439007927 25.507229586814358 26.16216917434324 24.536786848763136 66-67 23.9004668370205 25.510133648095973 25.77981622736444 24.80958328751909 68-69 23.860545267739518 25.21226786508373 26.38566394973974 24.541522917437007 70-71 23.997563607567386 25.156757780482568 26.202491821747927 24.643186790202122 72-73 23.883668608876807 25.118553455301125 26.18885637416716 24.80892156165491 74-75 23.74538256378376 25.809421651370034 25.868570627693792 24.576625157152414 76-77 23.942388555980827 25.24754158421063 26.094907478080344 24.715162381728202 78-79 23.92098429642684 25.182231180604102 26.258844426558518 24.63794009641054 80-81 23.972861252145584 25.559689842881667 26.03497870722539 24.432470197747364 82-83 23.984161726600735 25.37949765686516 26.179944430762188 24.45639618577192 84-85 23.79776991079227 25.1843434501102 26.088977409819304 24.92890922927823 86-87 23.879008693770064 25.303262175579196 26.154151393139884 24.663577737510856 88-89 23.94531617188229 25.152923735324613 25.87897978926615 25.022780303526947 90-91 23.69534276835043 24.901875151545006 26.41265960572456 24.990122474380005 92-93 23.67725427536478 25.08802578713266 26.494842642848077 24.739877294654484 94-95 23.498285742435012 24.908406991076426 26.586343040926057 25.006964225562506 96-97 23.310315065575352 24.920472150856266 26.71573745992386 25.053475323644527 98-99 23.200913438453235 25.24893348913505 26.240955127440067 25.309197944971647 100-101 23.181290688537214 24.929824470105796 26.573515611041437 25.31536923031555 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 28.0 1 20.5 2 18.0 3 22.5 4 26.5 5 34.0 6 50.0 7 99.5 8 158.5 9 204.5 10 219.0 11 206.0 12 210.5 13 233.0 14 264.0 15 284.5 16 317.5 17 356.5 18 387.0 19 404.5 20 443.0 21 509.5 22 614.5 23 776.0 24 891.0 25 1095.5 26 1490.0 27 2044.5 28 2721.0 29 3715.5 30 5242.0 31 7502.0 32 10681.0 33 13382.0 34 15773.0 35 19024.5 36 23651.0 37 27860.0 38 30688.5 39 33046.0 40 35100.0 41 38005.5 42 40549.5 43 44150.0 44 48866.5 45 53220.0 46 56737.5 47 60556.0 48 69883.5 49 71722.5 50 64723.5 51 61246.5 52 59869.5 53 60184.0 54 60181.5 55 56979.0 56 51407.0 57 47320.5 58 45548.0 59 41253.5 60 34772.5 61 29318.0 62 24317.0 63 18912.5 64 13977.0 65 10845.0 66 8206.0 67 6362.5 68 5057.0 69 3804.0 70 3000.5 71 2331.5 72 1863.0 73 1692.5 74 1385.5 75 1088.0 76 808.5 77 508.5 78 343.0 79 225.5 80 163.0 81 115.0 82 74.0 83 50.0 84 31.0 85 20.0 86 12.5 87 10.0 88 6.5 89 5.5 90 5.5 91 3.0 92 3.5 93 3.5 94 3.5 95 3.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07769608897312132 2 0.031841522177377395 3 0.016718533430823426 4 0.02351694121597154 5 0.02906666185690878 6 0.0354488405939866 7 0.08130340738973052 8 0.05917389633399329 9 0.07464374262060584 10-11 0.0653132747930301 12-13 0.04273284893521672 14-15 0.04370405004738074 16-17 0.055080977361302075 18-19 0.05247954581086274 20-21 0.020221794585415058 22-23 0.014602702436466105 24-25 0.02365568423199497 26-27 0.02604900125839916 28-29 0.006243435721054392 30-31 0.0010058868661698742 32-33 0.011203498543892047 34-35 0.012833728982167359 36-37 0.004925377068831798 38-39 0.009295782073569871 40-41 0.005168177346872802 42-43 0.0024973742884217562 44-45 4.1622904807029276E-4 46-47 4.5091480207615046E-4 48-49 0.001040572620175732 50-51 7.977723421347278E-4 52-53 6.937150801171546E-5 54-55 2.428002780410041E-4 56-57 0.003919490202661923 58-59 0.003711375678626777 60-61 0.006243435721054392 62-63 0.002323945518392468 64-65 4.856005560820082E-4 66-67 3.1217178605271953E-4 68-69 0.003954175956667781 70-71 0.002670803058451045 72-73 0.019874937045356478 74-75 0.016510418906788282 76-77 0.023725055740006686 78-79 0.019528079505297904 80-81 0.012486871442108784 82-83 0.004786634052808367 84-85 0.003260460876550627 86-87 0.0015261731762577402 88-89 0.0019077164703221751 90-91 0.007665551635294558 92-93 0.012140013902050205 94-95 0.015227046008571544 96-97 0.013527444062284516 98-99 0.02538997193228786 100-101 0.021262367205590788 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1441514.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.39852629103906 #Duplication Level Percentage of deduplicated Percentage of total 1 73.55293542299508 36.33406614275921 2 13.589199963036108 13.425729032964526 3 5.132412977769518 7.606009122564529 4 2.4140819007606713 4.770083529737903 5 1.3615266893637425 3.3628705980243105 6 0.8608743550326693 2.551555467621756 7 0.5706774591949818 1.9733437830222678 8 0.4024135204316414 1.5902907895129617 9 0.2856320985752163 1.2698824257929235 >10 1.633238632064515 14.712446920403865 >50 0.12753327314458013 4.316432176713916 >100 0.06466294410432072 5.874608832469565 >500 0.0035373261227983487 1.148732197214018 >1k 0.0012734374042074055 1.0639489811981615 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3659 0.25383034781486685 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2254 0.15636337905840667 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1848 0.12819854680565018 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1681 0.11661350496769368 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1453 0.10079680114102256 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.468575400585773E-5 0.0 20-21 0.0 0.0 6.937150801171546E-5 6.937150801171546E-5 0.0 22-23 0.0 0.0 6.937150801171546E-5 1.387430160234309E-4 0.0 24-25 0.0 0.0 6.937150801171546E-5 1.387430160234309E-4 0.0 26-27 0.0 0.0 6.937150801171546E-5 2.774860320468618E-4 0.0 28-29 0.0 0.0 6.937150801171546E-5 6.937150801171546E-4 0.0 30-31 0.0 0.0 6.937150801171546E-5 0.0013180586522225936 0.0 32-33 0.0 0.0 6.937150801171546E-5 0.002081145240351464 0.0 34-35 0.0 0.0 6.937150801171546E-5 0.0031564036145330533 0.0 36-37 0.0 0.0 6.937150801171546E-5 0.004405090758743932 0.0 38-39 0.0 0.0 6.937150801171546E-5 0.0061740642130426755 0.0 40-41 0.0 0.0 6.937150801171546E-5 0.010544469217780749 0.0 42-43 0.0 0.0 6.937150801171546E-5 0.01630230438275313 0.0 44-45 0.0 0.0 6.937150801171546E-5 0.03021129173910208 0.0 46-47 0.0 0.0 6.937150801171546E-5 0.05705806533963596 0.0 48-49 0.0 0.0 6.937150801171546E-5 0.07759203171110374 0.0 50-51 0.0 0.0 6.937150801171546E-5 0.10367571872350875 0.0 52-53 0.0 0.0 6.937150801171546E-5 0.13465009705073971 0.0 54-55 0.0 0.0 6.937150801171546E-5 0.17096608149487275 0.0 56-57 0.0 0.0 6.937150801171546E-5 0.2140804737241539 0.0 58-59 0.0 0.0 6.937150801171546E-5 0.2806424356613949 0.0 60-61 0.0 0.0 6.937150801171546E-5 0.36406167404548273 0.0 62-63 0.0 0.0 6.937150801171546E-5 0.46295075871618313 0.0 64-65 0.0 0.0 6.937150801171546E-5 0.5320794664498576 0.0 66-67 0.0 0.0 6.937150801171546E-5 0.653167433684307 0.0 68-69 0.0 0.0 6.937150801171546E-5 0.8100164132987956 0.0 70-71 0.0 0.0 6.937150801171546E-5 0.9179584797650249 0.0 72-73 0.0 0.0 6.937150801171546E-5 1.1023826338141705 0.0 74-75 0.0 0.0 6.937150801171546E-5 1.2428946232919 0.0 76-77 0.0 0.0 6.937150801171546E-5 1.3619708167940097 0.0 78-79 0.0 0.0 6.937150801171546E-5 1.5193053969645802 0.0 80-81 0.0 0.0 6.937150801171546E-5 1.679345465947608 0.0 82-83 0.0 0.0 6.937150801171546E-5 1.9003630904729334 0.0 84-85 0.0 0.0 6.937150801171546E-5 2.241046566318468 0.0 86-87 0.0 0.0 6.937150801171546E-5 2.688458107240027 0.0 88-89 0.0 0.0 6.937150801171546E-5 3.2320532440198293 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8665 0.0 33.342323 1 TACATGG 8695 0.0 32.67738 2 ACATGGG 8915 0.0 31.234682 3 TATTCCG 170 3.6270649E-9 27.952908 5 CATGGGA 4555 0.0 27.749432 4 ATGGGAG 2070 0.0 26.399965 5 CATGGGG 4035 0.0 25.908363 4 GTATAGT 185 8.934876E-9 25.685564 1 ATGGGGA 2220 0.0 23.545918 5 CCGTTGC 215 4.3941327E-8 22.106903 9 ATGTGCG 225 7.125709E-8 21.119242 7 GTGTAGC 860 0.0 20.996454 1 GGACCGT 205 6.8064764E-7 20.86169 6 GTATAGA 285 1.9554136E-9 20.007704 1 TACCTGG 1050 0.0 19.457846 2 GTCTATA 245 1.7496677E-7 19.395222 1 GTATAGG 370 7.2759576E-12 19.264174 1 TACATCG 175 9.907031E-5 19.007977 5 GTGTACG 175 9.909671E-5 19.007317 1 AGTACAT 5755 0.0 18.823708 2 >>END_MODULE