FastQCFastQC Report
Thu 26 May 2016
SRR936552_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936552_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441514
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46840.3249361435268752No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18470.12812917529763845No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17000.11793156361991627No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15650.10856641003833468No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14490.1005193151089757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG88800.030.1203021
TACATGG90950.028.9331422
ACATGGG91000.027.8162783
GTATAGG3350.026.9446581
CATGGGA46700.023.9981754
CATGGGG40550.022.250814
TCTATAC2457.537892E-921.3225823
ATGGGAG21600.020.6660375
ATGGGGA22300.019.8043775
GTAAGGT4900.019.382824
GTATAGT2702.2957465E-819.3549821
TAAGGTG5400.019.3469285
CATGGGC17500.019.2665254
GTATAGC4700.019.2052361
GTATACA3003.7107384E-919.0030751
ATGGGAT17300.018.9402645
GAGTACT24050.018.75821912-13
AGTACAT61550.018.5984842
CGTACAC1300.007572116318.2658483
TTCGCGA650.007576937318.26458248-49
Produced by FastQC (version 0.11.5)