FastQCFastQC Report
Thu 26 May 2016
SRR936551_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936551_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777661
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT75430.4243216226265863No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51910.2920129315994444No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39210.22057073874040103No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38080.2142140711868011No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24430.13742777728712055No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23340.13129612451417902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20540.11554508986809071No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC19370.10896340753383238No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17980.10114414390595282No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC17860.1004690995639776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG75700.028.3013761
TACATGG78600.027.3160822
ACATGGG77450.026.801713
CATGGGG36850.023.4604534
CATGGGA39350.020.1592434
GAGTACT33300.019.68868812-13
GTACTTT35850.018.28926714-15
GTACCTG15300.018.0079171
AGAGTAC78850.017.26400610-11
ATGGGGA21850.017.1761975
AGTACTT36600.017.13463212-13
TACCTGG12500.017.100352
ATGGGTA7550.016.9890165
ATGGGAG18600.016.857085
GTGTAGC9650.016.7370361
GTCCTAC4559.094947E-1216.7045781
GAGTACA65800.016.3158421
CATGGGC18800.016.1705444
AGTACAT56750.016.1545142
TATAAGG5650.015.9737772
Produced by FastQC (version 0.11.5)