##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936551_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1777661 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.407920857801347 31.0 31.0 34.0 30.0 34.0 2 31.504372318456667 31.0 31.0 34.0 30.0 34.0 3 31.52576447365386 33.0 31.0 34.0 28.0 34.0 4 35.14217615169596 37.0 35.0 37.0 32.0 37.0 5 35.14272350014991 37.0 35.0 37.0 32.0 37.0 6 35.11884380655254 37.0 35.0 37.0 32.0 37.0 7 35.02649155266386 37.0 35.0 37.0 32.0 37.0 8 34.97528212634467 37.0 35.0 37.0 32.0 37.0 9 36.48716150042106 39.0 37.0 39.0 32.0 39.0 10-11 36.340503898099804 39.0 37.0 39.0 32.0 39.0 12-13 36.25201374165266 39.0 35.0 39.0 32.0 39.0 14-15 37.37046602248685 40.0 37.0 41.0 32.0 41.0 16-17 37.26930809642559 40.0 37.0 41.0 32.0 41.0 18-19 37.18877783784422 39.5 37.0 41.0 31.0 41.0 20-21 37.02467933987414 39.0 36.0 41.0 31.0 41.0 22-23 37.15011776711083 39.0 37.0 41.0 31.0 41.0 24-25 37.19348486578712 39.0 37.0 41.0 31.0 41.0 26-27 37.106829142339286 39.0 36.5 41.0 31.0 41.0 28-29 36.96948968335357 39.0 36.0 41.0 30.5 41.0 30-31 36.79360406736718 39.0 36.0 41.0 30.0 41.0 32-33 36.6432286583325 39.0 36.0 41.0 30.0 41.0 34-35 36.45987958334013 39.0 35.5 40.5 30.0 41.0 36-37 36.27248896161866 39.0 35.0 40.0 30.0 41.0 38-39 36.080365716523005 39.0 35.0 40.0 29.5 41.0 40-41 35.88202418796385 38.0 35.0 40.0 28.5 41.0 42-43 35.66751000331334 38.0 35.0 40.0 27.5 41.0 44-45 35.454767810060524 38.0 34.5 40.0 27.0 41.0 46-47 35.364600168423564 38.0 34.5 40.0 27.0 41.0 48-49 35.26523898538585 38.0 34.0 40.0 26.0 41.0 50-51 34.33605282446991 37.5 33.5 39.5 25.0 40.5 52-53 34.48056069183045 38.0 33.5 39.5 26.0 40.5 54-55 34.90998930617255 38.0 34.0 40.0 26.0 41.0 56-57 34.78113852978717 38.0 34.0 40.0 26.0 41.0 58-59 34.53440897898981 38.0 34.0 40.0 25.5 41.0 60-61 34.2077398334103 37.5 33.5 40.0 24.5 41.0 62-63 33.84884913377748 37.0 33.0 40.0 23.0 41.0 64-65 33.47051743836423 36.5 32.5 39.0 22.5 41.0 66-67 32.98219964323907 36.0 32.0 39.0 22.0 41.0 68-69 32.5687344212423 35.0 31.5 39.0 21.0 40.0 70-71 32.147067973027475 35.0 31.0 38.0 20.0 40.0 72-73 31.744237793370054 35.0 31.0 37.0 19.0 39.5 74-75 31.29273860426707 35.0 31.0 37.0 18.0 39.0 76-77 30.81947879826356 34.5 30.0 36.0 14.0 39.0 78-79 30.304722891484936 34.0 30.0 36.0 9.5 38.0 80-81 29.801962803931684 34.0 29.0 35.0 7.0 37.0 82-83 29.376680649460162 34.0 29.0 35.0 4.5 37.0 84-85 28.991807211836228 34.0 29.0 35.0 2.0 36.0 86-87 28.617341832891647 34.0 29.0 35.0 2.0 36.0 88-89 28.186661855100606 34.0 27.5 35.0 2.0 35.5 90-91 27.754097659790027 33.0 27.0 35.0 2.0 35.0 92-93 27.259785752176597 33.0 26.0 35.0 2.0 35.0 94-95 26.72235566848797 33.0 25.0 35.0 2.0 35.0 96-97 26.080078260140713 32.5 24.5 35.0 2.0 35.0 98-99 25.243476399606 32.0 20.5 35.0 2.0 35.0 100-101 22.25964539920716 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5346558677344504 1101 2 0.47275723725795515 1101 3 0.4541815432447578 1101 4 0.5805421590838051 1101 5 0.43526388332837485 1101 6 0.4715792901289504 1101 7 0.5985825441374146 1101 8 0.5642343924850621 1101 9 0.6915185540304023 1101 10-11 0.7857425290005864 1101 12-13 0.7394274601128501 1101 14-15 0.8026466097583125 1101 16-17 0.9265312135953252 1101 18-19 0.9425946009154345 1101 20-21 0.8710301954433888 1101 22-23 0.8010928058171274 1101 24-25 0.6105620019393001 1101 26-27 0.6374401217842305 1101 28-29 0.6900767938438079 1101 30-31 0.6626225434252646 1101 32-33 0.5904574504295184 1101 34-35 0.39836515775610337 1101 36-37 0.24037044090734128 1101 38-39 0.27389711166870967 1101 40-41 0.33456606647030895 1101 42-43 0.4465466501387354 1101 44-45 0.23901892414063042 1101 46-47 0.4398786421792309 1101 48-49 0.706659491995147 1101 50-51 0.5383787785800962 1101 52-53 0.7127943954414704 1101 54-55 0.5238186417142074 1101 56-57 0.4452962295151721 1101 58-59 0.59956170057972 1101 60-61 0.5614296526943363 1101 62-63 0.36709125487534067 1101 64-65 0.3959439007239709 1101 66-67 0.38039592224492935 1101 68-69 0.28774927913106296 1101 70-71 0.21451541319389378 1101 72-73 0.22276947544964187 1101 74-75 0.39208699976791195 1101 76-77 0.3389546416640634 1101 78-79 0.22538360880310293 1101 80-81 0.45134550856225886 1101 82-83 0.3413565628560029 1101 84-85 0.3159252223167712 1101 86-87 0.2936869182834094 1101 88-89 0.2830999576609621 1101 90-91 0.21137090799066982 1101 92-93 0.22899213875197333 1101 94-95 0.20766922826627976 1101 96-97 0.04933716955480705 1101 98-99 0.10524132316645307 1101 100-101 0.4052922174719775 1102 1 0.2539613242625691 1102 2 0.2934855984452831 1102 3 0.2714535220005203 1102 4 0.3448125948511205 1102 5 0.38267733580366325 1102 6 0.357993927990222 1102 7 0.4207461715509524 1102 8 0.4830314795700943 1102 9 0.47903999362317506 1102 10-11 0.5931077051418754 1102 12-13 0.5485973146657699 1102 14-15 0.6217134438947767 1102 16-17 0.4447336210104993 1102 18-19 0.4151310620195474 1102 20-21 0.36272233944681176 1102 22-23 0.31772441125357176 1102 24-25 0.36669013841100195 1102 26-27 0.13029546716862228 1102 28-29 -0.14513535051280968 1102 30-31 0.31215688761101745 1102 32-33 0.28395173430849496 1102 34-35 0.31367551034772845 1102 36-37 0.018121717349508515 1102 38-39 -0.19160142370633793 1102 40-41 -0.028170762390416826 1102 42-43 -0.03145991473631682 1102 44-45 0.041775995067325766 1102 46-47 0.046250809228155276 1102 48-49 0.13875056142521203 1102 50-51 0.13007566854228259 1102 52-53 0.34182873631609567 1102 54-55 0.2423434432117233 1102 56-57 0.18915462776512015 1102 58-59 0.140582236232909 1102 60-61 0.16866757091815288 1102 62-63 0.1943951971115112 1102 64-65 0.21236072393369199 1102 66-67 0.19557890654794363 1102 68-69 0.22923238020673864 1102 70-71 0.1530159551161674 1102 72-73 -0.22290044422224398 1102 74-75 -0.01927383216554901 1102 76-77 0.12648402797848135 1102 78-79 0.3329586004700822 1102 80-81 0.46429822336103754 1102 82-83 0.5031745520263868 1102 84-85 0.12330408447867569 1102 86-87 0.28212697682425514 1102 88-89 0.06427450519450062 1102 90-91 0.1833256028475958 1102 92-93 0.09295055532353302 1102 94-95 -0.21986064368113034 1102 96-97 -0.2599150218640567 1102 98-99 0.0036940302392522995 1102 100-101 0.23199099478055274 1103 1 0.21790287520873974 1103 2 -0.00956204041471409 1103 3 0.16206888565713484 1103 4 0.3389624426946938 1103 5 0.3556875200666596 1103 6 0.3674187895950709 1103 7 0.4727821957118863 1103 8 0.2768381240100837 1103 9 0.15911638480049817 1103 10-11 0.20505509105217357 1103 12-13 -0.024062555435172328 1103 14-15 0.3421170404197582 1103 16-17 0.36573470534364105 1103 18-19 0.5907681767729187 1103 20-21 0.4684554057163197 1103 22-23 0.17282936052555442 1103 24-25 0.31246335630304856 1103 26-27 0.18853246391562095 1103 28-29 0.32929456916553335 1103 30-31 0.20153638303712285 1103 32-33 0.15037797756362892 1103 34-35 0.5640494580014987 1103 36-37 0.4905767331726736 1103 38-39 0.45122299256711784 1103 40-41 0.295113197579461 1103 42-43 0.23778941468924586 1103 44-45 0.46394586717492103 1103 46-47 0.5858129752066006 1103 48-49 0.6550669906370317 1103 50-51 0.6542571949298761 1103 52-53 0.6913268870586577 1103 54-55 0.6778772839786029 1103 56-57 0.7702147428995758 1103 58-59 0.6410554813713603 1103 60-61 0.6815139960246128 1103 62-63 0.7890806990804222 1103 64-65 0.8384335509871548 1103 66-67 0.8331851997302664 1103 68-69 0.6404650313368236 1103 70-71 0.7499267332770643 1103 72-73 0.6259500291839792 1103 74-75 0.4870753387522342 1103 76-77 0.4489121655949013 1103 78-79 0.2098756287345438 1103 80-81 0.49238525904462094 1103 82-83 0.17393458394885997 1103 84-85 0.49291555631823414 1103 86-87 0.486280534228559 1103 88-89 0.3277916198005961 1103 90-91 0.2276998422955998 1103 92-93 0.26212815583606286 1103 94-95 0.3460955531262542 1103 96-97 0.4331028346541821 1103 98-99 0.4029044368917951 1103 100-101 0.5184302951371507 1104 1 0.38651063764340776 1104 2 0.4653635339776372 1104 3 0.330874931616588 1104 4 0.49033163200481766 1104 5 0.45164540837483713 1104 6 0.5445053772641018 1104 7 0.6459162142109562 1104 8 0.5626226313901626 1104 9 0.8219887899821714 1104 10-11 0.7660539814706695 1104 12-13 0.677595190572454 1104 14-15 0.45508624424211774 1104 16-17 0.4743487261807928 1104 18-19 0.4787478112660821 1104 20-21 0.36217795591959856 1104 22-23 0.4018205030216251 1104 24-25 0.4476740760092568 1104 26-27 0.42785450558589844 1104 28-29 0.39220574923216844 1104 30-31 0.40161415956075786 1104 32-33 0.3599159190027237 1104 34-35 0.4042704773652588 1104 36-37 0.2929433616775299 1104 38-39 0.25577174763884614 1104 40-41 -0.02809915173009614 1104 42-43 0.3619749909359413 1104 44-45 0.2614716818254337 1104 46-47 0.14217097296123882 1104 48-49 0.417814454522734 1104 50-51 0.30598755251543963 1104 52-53 0.32062034264415473 1104 54-55 0.17586131749380485 1104 56-57 0.1734405412238189 1104 58-59 0.2208099272558357 1104 60-61 0.21961932765947978 1104 62-63 0.2942931370758828 1104 64-65 0.3318881790808277 1104 66-67 0.3232755585654985 1104 68-69 0.23334007693951264 1104 70-71 0.2705659822284616 1104 72-73 0.2550357531733134 1104 74-75 0.3573290056117244 1104 76-77 0.24602062743880282 1104 78-79 0.13107757962271194 1104 80-81 0.28046451400246397 1104 82-83 0.017761869172783662 1104 84-85 0.03721441368107392 1104 86-87 0.14712021834551692 1104 88-89 0.3413013540855552 1104 90-91 0.10846253596846367 1104 92-93 0.025877493211321934 1104 94-95 0.12350214067434706 1104 96-97 0.23336770818608343 1104 98-99 0.2096941485215389 1104 100-101 0.23312524588624228 1105 1 0.34407240115955773 1105 2 0.30651742157345296 1105 3 0.35826172239789145 1105 4 0.345928272803981 1105 5 0.356536883928257 1105 6 0.38090596018939493 1105 7 0.42180242497286 1105 8 0.5297046336104643 1105 9 0.6340069566373998 1105 10-11 0.6291349427162203 1105 12-13 0.38567004817775086 1105 14-15 0.5450429980158447 1105 16-17 0.5242301378639382 1105 18-19 0.5333791845684246 1105 20-21 0.6250378386093516 1105 22-23 0.25249154342805014 1105 24-25 0.4456916465727119 1105 26-27 0.2021540527567396 1105 28-29 0.3704949452874686 1105 30-31 0.3654291005829009 1105 32-33 0.23160459451921867 1105 34-35 0.32334561964423614 1105 36-37 0.3587664993472117 1105 38-39 0.15607154219548391 1105 40-41 0.21830880390916008 1105 42-43 0.27478358350361276 1105 44-45 0.48897506795069035 1105 46-47 0.4468719717363925 1105 48-49 0.564889040312238 1105 50-51 0.49892314503142643 1105 52-53 0.5657381376292179 1105 54-55 0.49786234972518884 1105 56-57 0.41839696969292817 1105 58-59 0.613490522102957 1105 60-61 0.5051955012697888 1105 62-63 0.5425333419997855 1105 64-65 0.4243603218198686 1105 66-67 0.6324692325866224 1105 68-69 0.762824503849231 1105 70-71 0.6072250087370605 1105 72-73 0.2995896268311746 1105 74-75 0.6125807463771231 1105 76-77 0.5146800612853433 1105 78-79 0.6468803118214161 1105 80-81 0.7813082487256828 1105 82-83 0.4640038037400096 1105 84-85 0.46855109675183826 1105 86-87 0.26133813848928256 1105 88-89 0.30817004391545666 1105 90-91 0.2568259966231956 1105 92-93 0.20686244870887904 1105 94-95 0.3571239536213682 1105 96-97 0.3621513029588854 1105 98-99 0.564629893789256 1105 100-101 0.4112669362307173 1106 1 0.43774819335351367 1106 2 0.37858395624717645 1106 3 0.47487420746250564 1106 4 0.40903774632783296 1106 5 0.36703154610916755 1106 6 0.42027949966301037 1106 7 0.5999703807112979 1106 8 0.6252824808133397 1106 9 0.6499153310725774 1106 10-11 0.7235571566726691 1106 12-13 0.5358434348642547 1106 14-15 0.5538638406690239 1106 16-17 0.5838385180258499 1106 18-19 0.6560531147389312 1106 20-21 0.40393853866122953 1106 22-23 0.7130749144299671 1106 24-25 0.6217213889084761 1106 26-27 0.40878333439599146 1106 28-29 0.7248638894718056 1106 30-31 0.7941540231746558 1106 32-33 0.757514881573286 1106 34-35 0.5770472877926238 1106 36-37 0.4505931949477997 1106 38-39 0.5480993407767016 1106 40-41 0.5483851251868757 1106 42-43 0.6255658112709526 1106 44-45 0.502990561275702 1106 46-47 0.5997196889089835 1106 48-49 0.609307668108265 1106 50-51 0.4333700784750718 1106 52-53 0.4267999969505354 1106 54-55 0.6049200802360772 1106 56-57 0.5880680882024691 1106 58-59 0.5984212814070915 1106 60-61 0.6932276221472975 1106 62-63 0.7743280179137386 1106 64-65 0.7100148183368447 1106 66-67 0.8842090763857016 1106 68-69 1.03283541800878 1106 70-71 0.7858024539563715 1106 72-73 0.5261468405457599 1106 74-75 0.8576513047784395 1106 76-77 0.8876841128126323 1106 78-79 0.7837033128427748 1106 80-81 0.8984371130915356 1106 82-83 0.7862927359593535 1106 84-85 0.9559255880869522 1106 86-87 1.0346205205449124 1106 88-89 1.1508210004194979 1106 90-91 0.8743842272505624 1106 92-93 0.8947309683918974 1106 94-95 1.0101791955037207 1106 96-97 1.1329731341691556 1106 98-99 1.2018103988652022 1106 100-101 1.0238819644112667 1107 1 0.18612092354194232 1107 2 0.1747392322668162 1107 3 0.1253256903761013 1107 4 0.27817323876000444 1107 5 0.2726011789896745 1107 6 0.30336575322769477 1107 7 0.5084062587700231 1107 8 0.48688697493730615 1107 9 0.5079454283868756 1107 10-11 0.44590255951582947 1107 12-13 0.36047151026355806 1107 14-15 0.12444556316522437 1107 16-17 0.07688111673934372 1107 18-19 0.18182177244650433 1107 20-21 0.25409922122427986 1107 22-23 0.14043079455341 1107 24-25 0.2136075429491484 1107 26-27 0.2832377937503452 1107 28-29 0.32987375777648253 1107 30-31 0.3480370546204128 1107 32-33 0.018982472181605203 1107 34-35 0.24950598909705235 1107 36-37 0.17698437071729245 1107 38-39 0.00968907449196621 1107 40-41 0.1878489482661223 1107 42-43 0.34777274466119223 1107 44-45 0.30134655913443 1107 46-47 0.3740507292075179 1107 48-49 0.39127497249804577 1107 50-51 0.2575805706027552 1107 52-53 0.41535241554064584 1107 54-55 0.3165364928825838 1107 56-57 0.11926054363269856 1107 58-59 0.3927735546550295 1107 60-61 0.6147498059686214 1107 62-63 0.3439411792104963 1107 64-65 0.09970570232002984 1107 66-67 0.25424817261951205 1107 68-69 0.3358445694621679 1107 70-71 0.3099306473809378 1107 72-73 0.1806485912056317 1107 74-75 0.3501114618245502 1107 76-77 0.26361664295547627 1107 78-79 0.2489292108932979 1107 80-81 0.15324947030850566 1107 82-83 0.3954610376771832 1107 84-85 0.3198781181485977 1107 86-87 0.33389636155472147 1107 88-89 0.1964245735403587 1107 90-91 0.1289473394522851 1107 92-93 0.07445025383732329 1107 94-95 0.25251741243475223 1107 96-97 0.36598517970660893 1107 98-99 0.3992772633426114 1107 100-101 0.3382541656608815 1108 1 0.271374159636693 1108 2 0.2569840153160641 1108 3 0.22249361860074757 1108 4 0.3380774889276381 1108 5 0.28418179758995166 1108 6 0.23313686957066437 1108 7 0.23295860322580353 1108 8 0.27882842877514946 1108 9 0.40719225528543745 1108 10-11 0.37537139753106885 1108 12-13 0.3233521794376131 1108 14-15 0.3698276459569456 1108 16-17 0.404772494776239 1108 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2312 32-33 0.23902362070882788 2312 34-35 0.3824080884463186 2312 36-37 0.4000325526787023 2312 38-39 0.37036335893215977 2312 40-41 0.2258842996841608 2312 42-43 0.3399317272431617 2312 44-45 0.048314158340836855 2312 46-47 -0.07079629226395667 2312 48-49 0.14839452218736682 2312 50-51 0.43029123317148077 2312 52-53 0.35301229666119127 2312 54-55 0.058481354924204254 2312 56-57 0.1613209606720929 2312 58-59 0.2837868671782289 2312 60-61 0.38240031451741885 2312 62-63 0.2553935740227402 2312 64-65 0.11646017565359301 2312 66-67 0.23996751750077294 2312 68-69 0.31158669823756924 2312 70-71 0.42274880747301324 2312 72-73 0.34124717410482575 2312 74-75 0.3767620424700553 2312 76-77 0.33126554935526187 2312 78-79 0.29659514680199806 2312 80-81 0.24792663060618736 2312 82-83 0.3010989666859629 2312 84-85 0.03882529930091749 2312 86-87 -0.18038446045453682 2312 88-89 -0.18789325811116342 2312 90-91 0.021322311748459555 2312 92-93 0.21091529895116778 2312 94-95 0.07463731593197309 2312 96-97 0.4385626970164971 2312 98-99 0.20404205451583834 2312 100-101 0.3169056969148052 2313 1 -0.2762081058940815 2313 2 -0.31279092318369806 2313 3 -0.23503946039956958 2313 4 -0.1276749721062771 2313 5 -0.10872925005173073 2313 6 -0.5142443335529023 2313 7 -0.25459041598029586 2313 8 -0.07716950050060234 2313 9 -0.48739681659026957 2313 10-11 -0.008652678053095997 2313 12-13 -0.058323208584148745 2313 14-15 -0.20524601090656347 2313 16-17 -0.41107237027838295 2313 18-19 -0.30847103689888655 2313 20-21 -0.4736056854971835 2313 22-23 -0.6640454105726477 2313 24-25 -0.3624009673605286 2313 26-27 -0.5005549848936113 2313 28-29 -0.5922952697351676 2313 30-31 -0.04986170156022496 2313 32-33 -0.23965016843885678 2313 34-35 -0.3272626189760999 2313 36-37 -0.06455738544245548 2313 38-39 -0.2642357266292308 2313 40-41 -0.09138450983528656 2313 42-43 -0.09978335824492746 2313 44-45 -0.19738471220227183 2313 46-47 -0.25999788413235336 2313 48-49 -0.3275777942071656 2313 50-51 -0.12490875827665349 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2314 9 -0.24267405753422366 2314 10-11 -0.386600133868896 2314 12-13 -0.7271683888820846 2314 14-15 -0.6604329141111691 2314 16-17 -0.8493228180556116 2314 18-19 -0.9983355575000346 2314 20-21 -1.3011711446448615 2314 22-23 -0.8930838918646415 2314 24-25 -0.6932945695030526 2314 26-27 -0.5405074963575984 2314 28-29 -0.5833046919341669 2314 30-31 -0.7206697549520058 2314 32-33 -0.5298332860296071 2314 34-35 -0.8149852236144781 2314 36-37 -0.8834849785199168 2314 38-39 -0.795144090284893 2314 40-41 -0.8199275961071493 2314 42-43 -1.1722788838738367 2314 44-45 -0.7727471685724794 2314 46-47 -1.0045385051634952 2314 48-49 -0.9814760921434811 2314 50-51 -0.6019091984010316 2314 52-53 -0.5560477490212179 2314 54-55 -0.9709700974722608 2314 56-57 -0.9523298156706161 2314 58-59 -0.9989248730862812 2314 60-61 -1.0136436878011352 2314 62-63 -1.0647690619555945 2314 64-65 -0.9991533373868364 2314 66-67 -0.7716975302907088 2314 68-69 -0.9492176794687239 2314 70-71 -0.8522360917932588 2314 72-73 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-1.2790869687513933 2315 30-31 -1.5822308291276812 2315 32-33 -1.472921239588672 2315 34-35 -1.447422969841007 2315 36-37 -1.4797236158619569 2315 38-39 -1.6930498688629712 2315 40-41 -1.54690875124232 2315 42-43 -1.7186837064875533 2315 44-45 -1.7071264785948372 2315 46-47 -2.1004311314518915 2315 48-49 -2.301808320799715 2315 50-51 -2.0761621708552624 2315 52-53 -2.2048234729114924 2315 54-55 -2.1374673595348384 2315 56-57 -2.1756676969156246 2315 58-59 -2.5934723128785855 2315 60-61 -2.560132030324347 2315 62-63 -2.79000774670083 2315 64-65 -2.2837688733153954 2315 66-67 -2.3595346774311885 2315 68-69 -2.0471210717636765 2315 70-71 -1.5769610807244518 2315 72-73 -1.6618047007087604 2315 74-75 -1.5123788718725812 2315 76-77 -1.5443995541221724 2315 78-79 -1.4355370066347497 2315 80-81 -1.5948144933843054 2315 82-83 -1.323697792169778 2315 84-85 -1.3576373636901558 2315 86-87 -1.1865099277462825 2315 88-89 -1.4282502530813517 2315 90-91 -1.3164865496378262 2315 92-93 -1.2732662780987631 2315 94-95 -1.2253695975245797 2315 96-97 -1.1635517710020693 2315 98-99 -1.3259905798947855 2315 100-101 -1.0699664454024997 2316 1 -4.524695379694684 2316 2 -2.974016884176365 2316 3 -2.5399339669045773 2316 4 -1.5540175520849289 2316 5 -1.7183252698505598 2316 6 -5.142628100553338 2316 7 -6.126122923983839 2316 8 -6.462269005529777 2316 9 -6.164784539951533 2316 10-11 -6.5283748813544165 2316 12-13 -4.4002429659803255 2316 14-15 -3.087589686405508 2316 16-17 -3.3436570744311425 2316 18-19 -3.908299613517187 2316 20-21 -4.2349504224587875 2316 22-23 -4.719115881476085 2316 24-25 -3.801903626693445 2316 26-27 -3.9859410706507106 2316 28-29 -2.9984750258825628 2316 30-31 -2.600059093881363 2316 32-33 -2.763490506290502 2316 34-35 -2.583076539462887 2316 36-37 -2.781556255864544 2316 38-39 -2.464922413503423 2316 40-41 -2.242283560195169 2316 42-43 -2.613404279844275 2316 44-45 -2.6680923161347962 2316 46-47 -2.6041399015925677 2316 48-49 -2.81992298810858 2316 50-51 -2.5112613001107746 2316 52-53 -2.412647122934633 2316 54-55 -2.4310595774577592 2316 56-57 -2.6671302772361543 2316 58-59 -2.611769114049622 2316 60-61 -2.7758071129320605 2316 62-63 -2.7072207588955592 2316 64-65 -2.686541512403103 2316 66-67 -2.658653304259591 2316 68-69 -2.8391277328514093 2316 70-71 -2.5539176995665436 2316 72-73 -2.513130998484005 2316 74-75 -2.2304849286055095 2316 76-77 -2.3601433195270296 2316 78-79 -3.2319626422028875 2316 80-81 -3.1591629082310924 2316 82-83 -3.211290591306568 2316 84-85 -2.7282034216070414 2316 86-87 -2.861883478747803 2316 88-89 -3.548550762181655 2316 90-91 -3.6422545726495876 2316 92-93 -4.40339966130168 2316 94-95 -4.33612464412932 2316 96-97 -4.732625407571188 2316 98-99 -5.724092822411659 2316 100-101 -6.199458576143332 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4569.0 3 1855.0 4 1859.0 5 2080.0 6 2260.0 7 2542.0 8 3106.0 9 3769.0 10 4886.0 11 5780.0 12 6715.0 13 7500.0 14 8343.0 15 9544.0 16 10936.0 17 12820.0 18 11588.0 19 10784.0 20 11709.0 21 12826.0 22 14316.0 23 16386.0 24 18925.0 25 21694.0 26 25640.0 27 29534.0 28 35765.0 29 42517.0 30 51392.0 31 63614.0 32 79819.0 33 102097.0 34 136297.0 35 183862.0 36 254579.0 37 317831.0 38 222928.0 39 24993.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.02028769595686 17.871063421835547 13.46487516822022 23.643773713987372 2 15.792482106549436 19.47935994274996 39.639970609845705 25.0881873408549 3 20.57984566255055 22.985995305512986 28.871488980558162 27.5626700513783 4 12.840137441886244 15.38777482891501 36.83023946553629 34.941848263662465 5 14.294725635352401 36.931018882193925 33.92346285346436 14.850792628989312 6 33.596086061179776 34.43899215962819 17.35608772706884 14.608834052123193 7 30.428462290570362 31.38803284941319 19.863509990627634 18.319994869388818 8 27.282395182086887 33.240415687364575 19.42655895422071 20.050630176327832 9 26.411104258298028 16.917269539145817 20.133389756495042 36.538236446061106 10-11 25.75560993686684 25.33460967152581 27.182570667801187 21.727209723806155 12-13 26.890261212622363 22.60790027434908 27.396896825220416 23.104941687808136 14-15 23.25857832311023 24.449395475873896 25.780800288996687 26.51122591201919 16-17 23.11768207866104 27.98155354884016 26.117720342302032 22.783044030196763 18-19 22.859966650621228 27.02863899705415 27.66912487280169 22.442269479522935 20-21 23.678559950740606 25.72117562898312 27.371157027258704 23.22910739301757 22-23 23.097648818473683 25.77908328647249 27.615981654364695 23.507286240689133 24-25 23.60599814583127 25.87541403767743 27.49134233715268 23.02724547933862 26-27 23.192054179729404 25.938956109761317 27.84884895077122 23.020140759738055 28-29 22.628055244519413 26.754264945415617 27.70655589185899 22.911123918205977 30-31 22.78869956826948 25.93207128546222 27.71724562172823 23.561983524540064 32-33 22.70780594277742 26.13623834115278 27.792469706686468 23.363486009383333 34-35 23.695497537353166 25.955631010533224 27.285834387387244 23.063037064726363 36-37 23.429145860938746 25.559395507172226 27.69639561771108 23.315063014177944 38-39 23.09247938723975 25.985016265755956 27.74744453526291 23.175059811741384 40-41 23.150118879893476 26.16004583619411 27.660297997141708 23.029537286770704 42-43 23.009608761043353 26.14741309402893 27.09051238568483 23.752465759242888 44-45 23.078662645580152 25.967032491031595 27.209333331083148 23.744971532305108 46-47 23.53549715423658 26.273083592454615 26.954716710474685 23.23670254283412 48-49 23.506008480840478 26.27299800748627 26.914264530667946 23.306728981005303 50-51 22.765622367417706 26.41784289916171 27.400036059784988 23.4164986736356 52-53 23.472255936597534 26.524017120870795 26.696497208449728 23.307229734081947 54-55 22.82800029478495 25.987065466449366 27.154306982521614 24.03062725624407 56-57 23.286365828995905 26.29115164845674 26.800335837110563 23.622146685436793 58-59 23.875219859149503 25.892621818072374 26.726687194823683 23.50547112795444 60-61 23.29862098509262 26.00877751834697 27.660236964305152 23.032364532255258 62-63 23.05587971975337 26.23083247334892 27.406627350682655 23.30666045621505 64-65 22.770518035191976 26.659698663881713 27.061240137608678 23.508543163317626 66-67 22.96722769238473 26.446076631786468 26.618466222133325 23.968229453695475 68-69 22.74364543596644 26.250843096652854 27.225754059737252 23.779757407643455 70-71 23.11583186950925 26.15958224590106 27.00044857460775 23.724137309981938 72-73 23.054535266071213 25.746936763868987 27.594963344125254 23.603564625934542 74-75 22.587940370063485 26.805139192320194 27.051238585863985 23.555681851752336 76-77 22.928333010727034 26.211180333920026 26.8864028197291 23.974083835623837 78-79 23.043373032847782 26.179587770448254 27.146109597676567 23.6309295990274 80-81 23.17497161841069 26.483055916727444 26.86687240093467 23.47510006392719 82-83 23.03636236238433 26.272727707619808 27.2411131437647 23.449796786231158 84-85 22.88820741566769 26.49943722664842 26.874263126994357 23.73809223068953 86-87 23.013662173749633 26.39232415619957 26.98813829392185 23.60587537612895 88-89 22.841550992643057 26.353309415037018 26.53392040344331 24.271219188876614 90-91 22.872824301141286 26.057626211566603 26.98222524481756 24.087324242474548 92-93 23.190147630803423 25.96656327587056 27.26214138515533 23.581147708170686 94-95 22.7696805960946 25.843124590702566 27.28773356677821 24.099461246424625 96-97 22.420001007117055 25.853679395257156 27.66325494042959 24.063064657196204 98-99 22.575826733851837 26.303579338140153 26.80743997149902 24.31315395650899 100-101 22.37686046249803 25.90743284321452 27.165201188894645 24.55050550539281 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 37.0 1 30.0 2 44.0 3 66.0 4 55.0 5 57.5 6 109.5 7 187.5 8 298.0 9 412.5 10 426.0 11 397.0 12 427.5 13 491.5 14 571.0 15 651.5 16 718.0 17 761.0 18 829.0 19 920.0 20 1051.5 21 1227.5 22 1467.5 23 1803.5 24 2308.5 25 3077.5 26 4110.0 27 5428.0 28 7152.5 29 9466.0 30 12581.0 31 16366.0 32 20803.0 33 24482.5 34 28514.5 35 32637.0 36 36399.0 37 40606.5 38 44210.0 39 46874.0 40 49421.5 41 53621.5 42 58391.0 43 62183.0 44 64667.5 45 68098.5 46 69896.0 47 75949.0 48 92057.0 49 93023.0 50 79188.0 51 71371.0 52 67119.5 53 64928.0 54 63205.0 55 58933.5 56 53166.0 57 47564.5 58 43168.0 59 38218.0 60 32382.0 61 26999.0 62 22025.0 63 17354.0 64 13274.0 65 10451.0 66 7910.5 67 6281.0 68 4762.5 69 3122.0 70 2394.5 71 1876.0 72 1495.5 73 1309.5 74 1094.0 75 861.0 76 617.0 77 395.5 78 268.0 79 184.0 80 121.0 81 89.0 82 59.5 83 39.0 84 25.5 85 14.5 86 13.0 87 12.0 88 8.0 89 5.0 90 2.5 91 2.5 92 3.0 93 1.0 94 0.5 95 0.5 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012882096192693657 2 0.01096947055709722 3 0.014457199657302489 4 0.01845121201398917 5 0.030208234303390803 6 0.04680307437694814 7 0.005344101040637107 8 0.025876699775716522 9 0.03448351513590049 10-11 0.039518220853132294 12-13 0.030686390712289915 14-15 0.02688926628867934 16-17 0.02908316040009878 18-19 0.02342966403605637 20-21 0.05310348823538347 22-23 0.013247745211263564 24-25 0.0025314162824070507 26-27 0.0012375812936212247 28-29 2.8126847582300564E-5 30-31 0.0026720505203185535 32-33 0.002306401501748646 34-35 2.8126847582300564E-5 36-37 3.093953234053062E-4 38-39 0.0 40-41 0.0014907229218619298 42-43 0.0036283633381167723 44-45 0.001800118245267236 46-47 0.0010406933605451208 48-49 3.9377586615220786E-4 50-51 0.0032345874719645652 52-53 0.0036283633381167723 54-55 0.005287847345472506 56-57 7.594248847221152E-4 58-59 0.005766003754371616 60-61 0.0028970653009769576 62-63 7.03171189557514E-4 64-65 6.469174943929129E-4 66-67 0.016397952140481228 68-69 0.04103707062257652 70-71 0.051865906941762234 72-73 0.014147804333897182 74-75 0.030011346370314704 76-77 0.02638298303219793 78-79 0.05518487495647371 80-81 0.0799646276764805 82-83 0.047112469700353446 84-85 0.01701674278729184 86-87 0.019407524831787388 88-89 0.020279457106838705 90-91 0.037943117388523455 92-93 0.023823439902208577 94-95 0.012657081412035254 96-97 0.017466772348608647 98-99 0.04950325174484899 100-101 0.06868576179597798 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1777661.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.112474909540126 #Duplication Level Percentage of deduplicated Percentage of total 1 79.48883772990797 45.39804250437876 2 12.046338239440601 13.759923809037703 3 3.677713964803137 6.301300396178536 4 1.5449900867419886 3.529528302581601 5 0.8498419869669369 2.4268289578861455 6 0.501025294581294 1.716887673949146 7 0.3357162094399387 1.3421508511855094 8 0.23686778536718933 1.0822484358929547 9 0.17810515658356474 0.9154823657974702 >10 0.9909728785956976 10.68034195806666 >50 0.09067825034292232 3.584639577045143 >100 0.05356635777545958 5.445419626344864 >500 0.003465038534539992 1.3220080100938547 >1k 0.0016830187167765675 1.7751919714685709 >5k 1.9800220197371384E-4 0.7200055600930304 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7543 0.4243216226265863 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5191 0.2920129315994444 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3921 0.22057073874040103 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3808 0.2142140711868011 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2443 0.13742777728712055 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2334 0.13129612451417902 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2054 0.11554508986809071 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1937 0.10896340753383238 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1798 0.10114414390595282 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1786 0.1004690995639776 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.625369516460113E-5 2 0.0 0.0 0.0 0.0 5.625369516460113E-5 3 0.0 0.0 0.0 0.0 5.625369516460113E-5 4 0.0 0.0 0.0 0.0 5.625369516460113E-5 5 0.0 0.0 0.0 0.0 5.625369516460113E-5 6 0.0 0.0 0.0 0.0 5.625369516460113E-5 7 0.0 0.0 0.0 0.0 5.625369516460113E-5 8 0.0 0.0 0.0 0.0 5.625369516460113E-5 9 0.0 0.0 0.0 0.0 1.1250739032920225E-4 10-11 0.0 0.0 0.0 5.625369516460113E-5 1.1250739032920225E-4 12-13 0.0 0.0 0.0 1.1250739032920225E-4 1.1250739032920225E-4 14-15 0.0 0.0 0.0 1.4063423791150283E-4 1.9688793307610393E-4 16-17 0.0 0.0 0.0 1.6876108549380338E-4 2.250147806584045E-4 18-19 0.0 0.0 0.0 1.6876108549380338E-4 2.250147806584045E-4 20-21 0.0 0.0 0.0 2.5314162824070503E-4 2.250147806584045E-4 22-23 0.0 0.0 0.0 4.2190271373450844E-4 2.250147806584045E-4 24-25 0.0 0.0 0.0 5.344101040637107E-4 2.250147806584045E-4 26-27 0.0 0.0 0.0 9.844396653805197E-4 2.250147806584045E-4 28-29 0.0 0.0 0.0 0.0016313571597734327 2.250147806584045E-4 30-31 0.0 0.0 0.0 0.0036846170332813736 2.250147806584045E-4 32-33 0.0 0.0 0.0 0.006806697114916736 2.250147806584045E-4 34-35 0.0 0.0 0.0 0.010660075233691913 2.812684758230056E-4 36-37 0.0 0.0 0.0 0.014850975523454697 2.812684758230056E-4 38-39 0.0 0.0 0.0 0.019407524831787388 2.812684758230056E-4 40-41 0.0 0.0 0.0 0.023851566749790876 2.812684758230056E-4 42-43 0.0 0.0 0.0 0.02953318996141559 2.812684758230056E-4 44-45 0.0 0.0 0.0 0.035102305782711105 2.812684758230056E-4 46-47 0.0 0.0 0.0 0.04356848690498357 2.812684758230056E-4 48-49 0.0 0.0 0.0 0.053215995625712664 2.812684758230056E-4 50-51 0.0 0.0 0.0 0.060135200130958605 2.812684758230056E-4 52-53 0.0 0.0 0.0 0.07357983327529827 2.812684758230056E-4 54-55 0.0 0.0 0.0 0.10367556018835988 2.812684758230056E-4 56-57 0.0 0.0 0.0 0.1602949043715309 2.812684758230056E-4 58-59 0.0 0.0 0.0 0.21803932245799396 2.812684758230056E-4 60-61 0.0 0.0 0.0 0.27156471340711197 2.812684758230056E-4 62-63 0.0 0.0 0.0 0.33347190493575546 2.812684758230056E-4 64-65 0.0 0.0 0.0 0.41329589837432446 3.3752217098760676E-4 66-67 0.0 2.8126847582300564E-5 0.0 0.5514268468510025 3.3752217098760676E-4 68-69 0.0 5.625369516460113E-5 0.0 0.7190066047463493 3.3752217098760676E-4 70-71 0.0 5.625369516460113E-5 0.0 0.8621160052450946 3.6564901856990734E-4 72-73 0.0 5.625369516460113E-5 0.0 1.0053097862865867 4.50029561316809E-4 74-75 0.0 5.625369516460113E-5 0.0 1.1584885982197957 4.50029561316809E-4 76-77 0.0 5.625369516460113E-5 0.0 1.3374034756908095 4.781564088991096E-4 78-79 0.0 5.625369516460113E-5 0.0 1.476800132308691 5.062832564814102E-4 80-81 0.0 5.625369516460113E-5 0.0 1.5849478612626366 5.062832564814102E-4 82-83 0.0 5.625369516460113E-5 0.0 1.7248508011369996 5.062832564814102E-4 84-85 0.0 5.625369516460113E-5 0.0 1.96705108566819 5.062832564814102E-4 86-87 0.0 5.625369516460113E-5 0.0 2.308145366298749 5.344101040637107E-4 88-89 0.0 5.625369516460113E-5 0.0 2.7326357500108287 5.625369516460112E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7570 0.0 28.301376 1 TACATGG 7860 0.0 27.316082 2 ACATGGG 7745 0.0 26.80171 3 CATGGGG 3685 0.0 23.460453 4 CATGGGA 3935 0.0 20.159243 4 GAGTACT 3330 0.0 19.688688 12-13 GTACTTT 3585 0.0 18.289267 14-15 GTACCTG 1530 0.0 18.007917 1 AGAGTAC 7885 0.0 17.264006 10-11 ATGGGGA 2185 0.0 17.176197 5 AGTACTT 3660 0.0 17.134632 12-13 TACCTGG 1250 0.0 17.10035 2 ATGGGTA 755 0.0 16.989016 5 ATGGGAG 1860 0.0 16.85708 5 GTGTAGC 965 0.0 16.737036 1 GTCCTAC 455 9.094947E-12 16.704578 1 GAGTACA 6580 0.0 16.315842 1 CATGGGC 1880 0.0 16.170544 4 AGTACAT 5675 0.0 16.154514 2 TATAAGG 565 0.0 15.973777 2 >>END_MODULE