FastQCFastQC Report
Thu 26 May 2016
SRR936551_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936551_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777661
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103990.5849821760166871No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT37390.21033256622044358No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35280.19846303654071276No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30670.17253008306983164No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT28450.1600417627432902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26640.1498598439184974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24560.13815907532426036No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23960.1347838536143843No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23950.13472759991921968No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22900.1288209619269366No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG22720.12780839541397376No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA19090.10738830406922355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG74400.025.4834941
ACATGGG76200.023.3769933
TACATGG77900.023.3599242
AATTCGC4800.022.26658678-79
ATTCGCG4600.022.20142280-81
CGTATAG1100.002871625621.5991331
CGCGGAA4650.020.94117782-83
CCGTCTA1404.8545204E-420.3563069
CTCGCGC4700.020.21193360-61
CGATCGT5300.019.7161724-25
GTATAGG6050.019.6355741
GTGTAGG10650.019.6318871
CAATTCG5600.019.50977178-79
CGCGATC5300.019.26807422-23
GAGTACT43950.019.2369812-13
GTACTTT44800.019.08403614-15
GTAAGGT6250.018.9997524
GAGTGCA12850.018.8513649
TCGCGCT5200.018.72519160-61
CCACGTC6400.018.5539259
Produced by FastQC (version 0.11.5)