##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936551_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1777661 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.075406390757294 31.0 31.0 34.0 31.0 34.0 2 32.193447457079834 33.0 31.0 34.0 30.0 34.0 3 32.335391843551726 34.0 31.0 34.0 30.0 34.0 4 36.01674616251355 37.0 35.0 37.0 35.0 37.0 5 35.89848345663206 37.0 35.0 37.0 35.0 37.0 6 35.95446375883816 37.0 35.0 37.0 35.0 37.0 7 35.886990826709926 37.0 35.0 37.0 35.0 37.0 8 35.852963529041816 37.0 35.0 37.0 35.0 37.0 9 37.501853277987195 39.0 37.0 39.0 35.0 39.0 10-11 37.39375870877518 39.0 37.0 39.0 34.0 39.0 12-13 37.28598112913542 39.0 37.0 39.0 34.0 39.0 14-15 38.43529474967387 40.0 38.0 41.0 34.0 41.0 16-17 38.41990627009312 40.0 38.0 41.0 33.5 41.0 18-19 38.52873607510093 40.0 38.0 41.0 34.0 41.0 20-21 38.52422452874873 40.0 38.0 41.0 34.0 41.0 22-23 38.432211765910374 40.0 38.0 41.0 34.0 41.0 24-25 38.312381550813114 40.0 38.0 41.0 34.0 41.0 26-27 38.1322020902748 40.0 38.0 41.0 33.5 41.0 28-29 38.185570252145936 40.0 38.0 41.0 34.0 41.0 30-31 38.12944059637917 40.0 38.0 41.0 33.5 41.0 32-33 37.9964017323888 40.0 37.0 41.0 33.0 41.0 34-35 37.834004064892014 40.0 37.0 41.0 33.0 41.0 36-37 37.681231967174845 40.0 37.0 41.0 33.0 41.0 38-39 37.504326752963586 40.0 37.0 41.0 32.5 41.0 40-41 37.32467242066963 39.5 36.5 41.0 32.0 41.0 42-43 37.10190159991134 39.0 36.0 41.0 31.0 41.0 44-45 36.876915227369 39.0 36.0 40.5 31.0 41.0 46-47 36.592096862112626 39.0 35.5 40.0 30.5 41.0 48-49 36.267954632519924 39.0 35.0 40.0 30.0 41.0 50-51 36.00576009711638 38.5 35.0 40.0 29.5 41.0 52-53 35.76328726343212 38.0 35.0 40.0 29.0 41.0 54-55 35.4278546359514 38.0 34.0 40.0 28.0 41.0 56-57 35.07945693807762 38.0 34.0 40.0 27.5 41.0 58-59 34.71005214155005 37.5 34.0 40.0 26.5 41.0 60-61 34.31326979665977 37.0 33.0 39.5 26.0 41.0 62-63 34.0042055262505 36.0 33.0 39.0 26.0 40.0 64-65 33.68271312696852 36.0 32.5 39.0 26.0 40.0 66-67 33.223811514118836 35.5 32.0 39.0 25.0 40.0 68-69 32.75709738808468 35.0 31.5 38.0 23.5 40.0 70-71 32.24533699057356 35.0 31.0 37.5 22.5 39.5 72-73 31.674791762883924 34.5 31.0 37.0 21.5 39.0 74-75 31.134859233565905 34.0 30.0 36.0 20.0 39.0 76-77 29.760196966688248 32.5 28.5 35.0 19.5 36.5 78-79 30.551561574450922 34.0 30.0 35.0 20.0 37.0 80-81 30.3869807010448 34.0 30.0 35.0 18.5 37.0 82-83 29.977850388797414 34.0 29.5 35.0 17.5 36.5 84-85 29.53444920038185 34.0 29.0 35.0 13.0 36.0 86-87 29.119644296634736 33.5 29.0 35.0 7.5 35.5 88-89 28.635191974172805 33.0 29.0 35.0 4.5 35.0 90-91 28.073835787588298 33.0 27.0 34.5 2.0 35.0 92-93 27.487683815980663 32.5 26.5 34.0 2.0 35.0 94-95 26.73892153790852 32.0 25.0 34.0 2.0 35.0 96-97 25.896024888884888 32.0 24.5 34.0 2.0 35.0 98-99 24.723713632689247 31.5 18.5 34.0 2.0 35.0 100-101 20.833310175562158 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22843872441708868 1101 2 0.36319062687946513 1101 3 0.2056634612559023 1101 4 0.15524030074803363 1101 5 0.2571652742326904 1101 6 0.2171566600974586 1101 7 0.188053692709083 1101 8 0.17109984448158144 1101 9 0.3186491046278235 1101 10-11 0.24262112609149256 1101 12-13 0.3509177456653987 1101 14-15 0.305612799242553 1101 16-17 0.37708959789644325 1101 18-19 0.34310514301017747 1101 20-21 0.3612822549332435 1101 22-23 0.3608818046958575 1101 24-25 0.28874225495372485 1101 26-27 0.31111413180585856 1101 28-29 0.21856059840003894 1101 30-31 0.32023781101607796 1101 32-33 0.3487030186177833 1101 34-35 0.3262742050610399 1101 36-37 0.32776905379347454 1101 38-39 0.37500092716400957 1101 40-41 0.28611926774292584 1101 42-43 0.3656139258612896 1101 44-45 0.4306620577579352 1101 46-47 0.3463470650139797 1101 48-49 0.30518042673159584 1101 50-51 0.2554537066616902 1101 52-53 0.28987779168593164 1101 54-55 0.25609338732767384 1101 56-57 0.35522379993494013 1101 58-59 0.23538047410207952 1101 60-61 0.1544715437875439 1101 62-63 0.24621090311893568 1101 64-65 0.31552242508504236 1101 66-67 0.2100314392512601 1101 68-69 0.12841561096239928 1101 70-71 -0.002519241964257901 1101 72-73 0.017651583305294594 1101 74-75 0.18081199028189943 1101 76-77 0.05299375093488479 1101 78-79 0.04412053875891431 1101 80-81 0.3902746654681799 1101 82-83 0.24170943939676093 1101 84-85 0.12978953998704768 1101 86-87 0.26309645931766923 1101 88-89 -0.07109154029677711 1101 90-91 -0.02261276347659802 1101 92-93 0.03626103497029476 1101 94-95 -0.015524794627442162 1101 96-97 0.07993198939758273 1101 98-99 -0.5570367680320167 1101 100-101 -0.0427903800254974 1102 1 0.25958192654857726 1102 2 0.3310977915112545 1102 3 0.2947106714047507 1102 4 0.20015119813157867 1102 5 0.11936492678436394 1102 6 0.12886659592768268 1102 7 0.19983713571453166 1102 8 0.12415546932567878 1102 9 0.20553149535488302 1102 10-11 0.3591248113836585 1102 12-13 0.1968521145742912 1102 14-15 0.32772598704786304 1102 16-17 0.3637601864174087 1102 18-19 0.32017498807118017 1102 20-21 0.14735143114517513 1102 22-23 0.2451860361043927 1102 24-25 0.14267610291086896 1102 26-27 0.2475403586361935 1102 28-29 0.4616077688152771 1102 30-31 0.39501701356255836 1102 32-33 0.27006457900072434 1102 34-35 0.3885325750559758 1102 36-37 0.41876067220955804 1102 38-39 0.3034350698120818 1102 40-41 0.34975827025276374 1102 42-43 0.3960434682404923 1102 44-45 0.5637221857443038 1102 46-47 0.3221140892095349 1102 48-49 0.4401220514388342 1102 50-51 0.455886140764612 1102 52-53 0.4262043533822961 1102 54-55 0.5458623143215888 1102 56-57 0.3866252705199358 1102 58-59 0.3304857350525552 1102 60-61 0.28121634585898647 1102 62-63 0.36383077168898836 1102 64-65 0.3792012715742459 1102 66-67 0.2875315109925012 1102 68-69 0.23213324186807682 1102 70-71 0.11742945753267264 1102 72-73 -0.0746302736669584 1102 74-75 0.08227186927098629 1102 76-77 0.06439884169306964 1102 78-79 0.08340041125818232 1102 80-81 0.19566784055873754 1102 82-83 0.27621421515572564 1102 84-85 0.16498843980758338 1102 86-87 0.24131427454314647 1102 88-89 -0.024637544089131325 1102 90-91 0.08584928099413602 1102 92-93 0.41297570572216813 1102 94-95 0.18325659768234104 1102 96-97 0.11406702185773199 1102 98-99 -0.07726393389996744 1102 100-101 -0.0819126425454435 1103 1 0.1303944031912323 1103 2 0.17244825571260947 1103 3 0.11264648956079526 1103 4 0.1280471868353672 1103 5 0.10414652294483062 1103 6 0.0244295746957377 1103 7 0.06698331449879191 1103 8 0.05129297712680625 1103 9 0.025708468095594128 1103 10-11 0.10378081175921494 1103 12-13 0.035593344638854774 1103 14-15 0.13927075214891005 1103 16-17 0.1566626829609774 1103 18-19 0.030075948322732415 1103 20-21 0.0959342040556237 1103 22-23 0.0913699750871757 1103 24-25 0.12943030171018677 1103 26-27 -0.029869889324466214 1103 28-29 -0.0011570551600854628 1103 30-31 0.23389712203269397 1103 32-33 0.1826211198859724 1103 34-35 0.2707244513941447 1103 36-37 0.19231511714697547 1103 38-39 0.21141748299639573 1103 40-41 0.27928328999720264 1103 42-43 0.23620638975428676 1103 44-45 0.20725526158687302 1103 46-47 0.19405864922617866 1103 48-49 0.07040460858724629 1103 50-51 0.19838521107320872 1103 52-53 0.21784877495776556 1103 54-55 0.092709210717139 1103 56-57 0.07001081078109195 1103 58-59 0.019846268456092275 1103 60-61 -0.10265127621133985 1103 62-63 0.15424599856130072 1103 64-65 0.1302485551264354 1103 66-67 0.05256604764719697 1103 68-69 0.20892743120479196 1103 70-71 0.020140359406958908 1103 72-73 0.03861097112539014 1103 74-75 0.06136221226267935 1103 76-77 -0.07153699855465234 1103 78-79 -0.17193162204134183 1103 80-81 0.11615322231211778 1103 82-83 0.04815999481702349 1103 84-85 0.19531812586644648 1103 86-87 0.08095654586207601 1103 88-89 0.06598838215044367 1103 90-91 0.0302441853647899 1103 92-93 0.13233984283047562 1103 94-95 -0.0466765657905448 1103 96-97 0.22487162373809966 1103 98-99 0.4354491357201127 1103 100-101 0.3198927307026409 1104 1 0.27118024085975634 1104 2 0.35509484442975037 1104 3 0.28407809157074126 1104 4 0.24063342883096084 1104 5 0.069791823700875 1104 6 0.19899516497093117 1104 7 0.2665084418336008 1104 8 0.23543059748263317 1104 9 0.31417899918302794 1104 10-11 0.31226460038040926 1104 12-13 0.3421047790727627 1104 14-15 0.4013709601576494 1104 16-17 0.38179787856377345 1104 18-19 0.34170521406397114 1104 20-21 0.2575584560203197 1104 22-23 0.22771647894803948 1104 24-25 0.2096940616294063 1104 26-27 0.13767036701808166 1104 28-29 0.17806114338748102 1104 30-31 0.2280400736202708 1104 32-33 0.3944211815629828 1104 34-35 0.4144650159201859 1104 36-37 0.33696194068018315 1104 38-39 0.4144958410173274 1104 40-41 0.38048221246355496 1104 42-43 0.3942258875502489 1104 44-45 0.32308685311576113 1104 46-47 0.23926162403333961 1104 48-49 0.3243759479256809 1104 50-51 0.2959468780052603 1104 52-53 0.3305385678157293 1104 54-55 0.34206787563159224 1104 56-57 0.3015291234109725 1104 58-59 0.3568097187809016 1104 60-61 0.17533731253401896 1104 62-63 0.19398370498277018 1104 64-65 0.18705716289895236 1104 66-67 0.39886910549660115 1104 68-69 0.21695866980412148 1104 70-71 0.2349016986043111 1104 72-73 0.37673124331046637 1104 74-75 0.6017814395666505 1104 76-77 0.45217614892797897 1104 78-79 0.47027860686905143 1104 80-81 0.4593694062789204 1104 82-83 0.4591153233277261 1104 84-85 0.5391816916844867 1104 86-87 0.4226494487731891 1104 88-89 0.40063037834904236 1104 90-91 0.3555129389046705 1104 92-93 0.3581575671100552 1104 94-95 0.3955070321559724 1104 96-97 0.5884783834174634 1104 98-99 0.3944321604332437 1104 100-101 0.12306307514657178 1105 1 0.14159534239980331 1105 2 0.23504355687924772 1105 3 0.18528192435336877 1105 4 0.10790811938655764 1105 5 0.19043431702056068 1105 6 0.11583547743822464 1105 7 0.15468928959541728 1105 8 0.11879410828313297 1105 9 0.16336521048496166 1105 10-11 0.17619238224271072 1105 12-13 0.16330849356280908 1105 14-15 0.15589314426051004 1105 16-17 0.22732357724221686 1105 18-19 0.2793039465846334 1105 20-21 0.29682632263669007 1105 22-23 0.132176315143667 1105 24-25 0.0859879189268824 1105 26-27 0.22309702908676599 1105 28-29 0.20441428841444775 1105 30-31 0.22735410520387234 1105 32-33 0.2949524181345211 1105 34-35 0.0821324121435083 1105 36-37 0.24094873511312898 1105 38-39 0.11097005336228705 1105 40-41 0.32209556389050675 1105 42-43 0.34014483192778755 1105 44-45 0.32385729786759754 1105 46-47 0.2918170910408975 1105 48-49 0.2724917913402507 1105 50-51 0.44307263819585074 1105 52-53 0.42883559175428587 1105 54-55 0.40748148951379193 1105 56-57 0.4996172580731937 1105 58-59 0.5072486999937311 1105 60-61 0.4767463058049728 1105 62-63 0.44396216801749233 1105 64-65 0.2532941960386168 1105 66-67 0.42229695203408113 1105 68-69 0.48883617628899145 1105 70-71 0.39921588576838474 1105 72-73 0.4662675440904529 1105 74-75 0.5387713510092169 1105 76-77 0.240304613647087 1105 78-79 0.30130793494402397 1105 80-81 0.49281856348282105 1105 82-83 0.3714693894939103 1105 84-85 0.4835376309880459 1105 86-87 0.42012480212728676 1105 88-89 0.4002586567573303 1105 90-91 0.43226399704470353 1105 92-93 0.4596175033400627 1105 94-95 0.640721904208398 1105 96-97 0.6410587947310695 1105 98-99 0.4619737550635854 1105 100-101 0.18746310158239154 1106 1 0.09232205417981021 1106 2 0.29275321799542553 1106 3 0.2175295684683718 1106 4 0.16384582805800108 1106 5 0.19314033915689777 1106 6 0.018408116646575934 1106 7 0.1448384683090751 1106 8 0.05716191045606678 1106 9 0.1587307236311517 1106 10-11 0.13894565829755834 1106 12-13 0.12805737059420608 1106 14-15 0.18305705847955522 1106 16-17 0.11438065168033518 1106 18-19 0.18535891436201268 1106 20-21 0.15399219395413155 1106 22-23 0.060270184699724894 1106 24-25 0.2252539569868901 1106 26-27 0.03379501822376341 1106 28-29 0.1187379969380018 1106 30-31 0.17778600123747168 1106 32-33 0.22890910398854203 1106 34-35 0.18854004682886227 1106 36-37 0.14592118283719913 1106 38-39 0.12346573745992373 1106 40-41 0.16811292424292645 1106 42-43 0.1885699237084708 1106 44-45 0.20191853058383202 1106 46-47 0.1085993210322016 1106 48-49 0.04042996037461677 1106 50-51 0.12032278706320909 1106 52-53 0.06466002779581714 1106 54-55 0.1579285085804898 1106 56-57 0.1350740431721178 1106 58-59 -0.07196680378397247 1106 60-61 0.002614476323842041 1106 62-63 0.10137139784923477 1106 64-65 0.05518732734602594 1106 66-67 -0.05079614191053139 1106 68-69 -0.010754195231662322 1106 70-71 0.04062361219271793 1106 72-73 0.11276358736011716 1106 74-75 0.28942561678874057 1106 76-77 -0.016131849320110803 1106 78-79 0.10576822892021909 1106 80-81 0.013244249064957359 1106 82-83 0.006233128241291297 1106 84-85 -0.02543444199615763 1106 86-87 0.23670770831833465 1106 88-89 0.15691626628395028 1106 90-91 0.47107848468801805 1106 92-93 0.21545001797243657 1106 94-95 0.39529703871721367 1106 96-97 0.5364902487495407 1106 98-99 0.7146952060296492 1106 100-101 0.6178843614533669 1107 1 0.025520330256867396 1107 2 0.10952318819042262 1107 3 0.024854907015310346 1107 4 -0.045970336706432136 1107 5 -0.009832389755580095 1107 6 0.06691969327281555 1107 7 0.03161395408312018 1107 8 0.03949088835721426 1107 9 0.07564006224305331 1107 10-11 0.033963048900893966 1107 12-13 -0.05763263066435087 1107 14-15 0.08871136796792456 1107 16-17 0.1050380959431223 1107 18-19 0.03515609648689377 1107 20-21 -0.02263117551368765 1107 22-23 0.02075288776264017 1107 24-25 0.07238730582493247 1107 26-27 0.01663205577204252 1107 28-29 0.13584658656790083 1107 30-31 0.042721736911893515 1107 32-33 -0.02792128238618119 1107 34-35 0.07296851162455908 1107 36-37 0.23588239399107636 1107 38-39 0.2808221337626833 1107 40-41 0.24440176311203743 1107 42-43 0.09277124640348688 1107 44-45 0.23995674177309212 1107 46-47 0.13070513547327778 1107 48-49 -0.049944339230940216 1107 50-51 -0.011690799472518165 1107 52-53 -0.03982583150066432 1107 54-55 -0.09399237446893238 1107 56-57 -0.018506414394757087 1107 58-59 0.0035690802485106587 1107 60-61 -0.25002833280522196 1107 62-63 -0.3191777299527132 1107 64-65 -0.27111992578541333 1107 66-67 -0.2046516534587326 1107 68-69 -0.2983439393865055 1107 70-71 -0.11367326167451353 1107 72-73 -0.18751793336216238 1107 74-75 -0.13084874702024507 1107 76-77 -0.21717311060718103 1107 78-79 -0.08655438663580384 1107 80-81 -0.14698085731728483 1107 82-83 -0.19020529243557505 1107 84-85 -0.11393077683298358 1107 86-87 0.14366667554211077 1107 88-89 0.027889415099608073 1107 90-91 0.0040031857244962055 1107 92-93 0.01712406291019164 1107 94-95 0.06607119750441015 1107 96-97 0.17142474327369328 1107 98-99 0.357750535233162 1107 100-101 -0.03246069572364263 1108 1 0.03225762904399687 1108 2 0.1650029792795209 1108 3 -0.0043429403450332416 1108 4 0.06412077371337688 1108 5 0.057781226591870904 1108 6 0.022084278970886828 1108 7 0.036520267207045265 1108 8 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-1.4325384213600394 2316 82-83 -1.7410615634647293 2316 84-85 -1.4560296163216329 2316 86-87 -1.7114578306760713 2316 88-89 -1.6263090020691138 2316 90-91 -2.0979148402896186 2316 92-93 -2.0625700591010627 2316 94-95 -2.0408455940551455 2316 96-97 -1.8559696023041106 2316 98-99 -1.3361979390058174 2316 100-101 -1.2770197907381835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 22.0 5 60.0 6 113.0 7 212.0 8 513.0 9 873.0 10 1459.0 11 2274.0 12 3245.0 13 4653.0 14 6173.0 15 7851.0 16 9821.0 17 12044.0 18 11305.0 19 8909.0 20 9385.0 21 10072.0 22 11224.0 23 12539.0 24 14825.0 25 17810.0 26 21958.0 27 27012.0 28 34208.0 29 42433.0 30 53566.0 31 66928.0 32 84650.0 33 111923.0 34 150226.0 35 207977.0 36 293939.0 37 357619.0 38 174355.0 39 5472.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.5477560802714 18.754409357119954 13.832469746324833 22.86536481628382 2 16.196572565332627 20.45692752833926 38.654365363117805 24.692134543210305 3 21.072688517083144 23.432481740532445 28.950010998154223 26.544818744230188 4 13.54771277714836 16.202160370554967 36.44311785506549 33.807008997231186 5 14.422489477255008 37.43695072733799 33.53624988608095 14.60430990932606 6 33.704401457870766 35.15096522902848 16.7546005678248 14.390032745275954 7 30.636168844720892 31.961677646475106 19.446664907797174 17.95548860100683 8 27.52572529235588 33.74524928725245 18.884941056283992 19.844084364107676 9 26.64003613741443 17.212299996793536 19.63244919301171 36.515214672780324 10-11 26.000964188335125 25.734996717596886 26.719942666233887 21.544096427834102 12-13 27.288161241091522 22.84279736125167 27.17157545786289 22.697465939793922 14-15 23.329892470698756 24.970705789368576 25.488213404738264 26.2111883351944 16-17 23.212744203184926 28.358186704770638 25.908384514448613 22.52068457759582 18-19 22.96265481269464 27.247231147954132 27.63606303216864 22.15405100718259 20-21 23.84078764616902 25.81319392839202 27.615319317458827 22.730699107980136 22-23 23.334161202775228 25.97145509914604 27.491434645239192 23.202949052839543 24-25 23.850830280150287 26.1205364601528 27.622432499481057 22.406200760215857 26-27 23.538921953579425 26.213702282776236 27.848266822677004 22.399108940967338 28-29 22.7881814328439 27.033733381599017 27.790205160096654 22.38788002546043 30-31 23.031616986901824 26.214354665592595 27.658000553539164 23.09602779396642 32-33 23.07947620608036 26.418458517979925 27.670928481118583 22.83113679482113 34-35 23.950172390612543 26.121860952302733 27.34718721187366 22.580779445211064 36-37 23.74812638402876 25.79853519725952 27.629468305126075 22.823870113585638 38-39 23.261995609192848 26.210699674135164 27.859010366636618 22.66829435003537 40-41 23.335424096846417 26.46585961319937 27.502756432613662 22.695959857340547 42-43 23.302181701787024 26.538816155495265 26.95098976232091 23.208012380396806 44-45 23.529360472148323 26.13017607676067 27.053736631380808 23.286726819710204 46-47 24.025615682630153 26.700703902487593 26.668076759292127 22.605603655590127 48-49 24.04364560573706 26.660998089597644 26.609750252582643 22.685606052082655 50-51 23.276845788129656 26.707135859535786 27.114527984581876 22.90149036775268 52-53 23.845404372159535 27.133383999275452 26.22319716345729 22.798014465107723 54-55 23.172885471036494 26.499338034083582 26.732819192876928 23.594957302003 56-57 23.693707353203035 26.594273243866894 26.647663932339537 23.064355470590534 58-59 24.492575931837354 26.254809421868043 26.23587972866127 23.016734917633332 60-61 23.61932241214279 26.44251607983621 27.39961335823937 22.538548149781633 62-63 23.33678918533961 26.599109729020327 27.19202367605522 22.872077409584843 64-65 23.162154149586158 27.07591818457469 26.718697172778523 23.04323049306063 66-67 23.416311069895546 26.800169100082105 26.418259433615983 23.36526039640637 68-69 23.16622038207961 26.613037510407057 26.884380414482123 23.33636169303121 70-71 23.625225480657395 26.592977070378655 26.684927330041802 23.096870118922148 72-73 23.547330508414984 26.222714762471995 27.224271608445505 23.005683120667516 74-75 23.17044184605414 27.338164505315877 26.52811856295746 22.963275085672528 76-77 23.462623193081413 26.61687799008408 26.48330250136909 23.43719631546542 78-79 23.51508271082336 26.643351784775493 26.58023114418538 23.26133436021577 80-81 23.745200406497545 26.91575189248348 26.434182247150623 22.90486545386835 82-83 23.53031515497569 26.79102787860718 26.589100298573793 23.089556667843336 84-85 23.343928880728228 26.932126335377905 26.510217094806098 23.21372768908777 86-87 23.45680297155188 26.885810929780042 26.354071679744745 23.303314418923335 88-89 23.150145461388107 27.048223762171826 25.870384522672037 23.93124625376803 90-91 23.410549576788828 26.56074502943815 26.25681383912209 23.771891554650935 92-93 23.731672542010372 26.51001208634097 26.520419221923163 23.23789614972549 94-95 23.334442472121285 26.45343825054967 26.576352954776674 23.635766322552374 96-97 22.99237367796636 26.52361898429339 26.9967974735312 23.487209864209053 98-99 23.221759440521677 27.026995099164292 25.972544744314984 23.778700715999047 100-101 22.64759712978118 27.2108242723687 25.951425897730946 24.190152700119175 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 82.0 1 65.5 2 53.5 3 65.5 4 65.0 5 70.5 6 120.0 7 235.0 8 373.0 9 457.0 10 461.0 11 456.0 12 484.0 13 560.0 14 685.0 15 788.0 16 916.5 17 1008.5 18 1091.5 19 1205.5 20 1336.5 21 1486.0 22 1681.5 23 2066.5 24 2555.5 25 3283.0 26 4339.0 27 5743.0 28 7534.0 29 9899.0 30 12995.5 31 16644.5 32 20930.0 33 24632.5 34 28302.5 35 32309.0 36 36812.5 37 40699.5 38 44001.0 39 46784.0 40 49412.5 41 54049.5 42 58703.0 43 62160.5 44 64439.0 45 67129.5 46 68815.0 47 75956.0 48 93665.0 49 93882.0 50 78872.5 51 70978.0 52 66336.0 53 64322.5 54 62138.0 55 57743.5 56 51992.5 57 46609.5 58 42957.0 59 38410.5 60 32656.0 61 26798.5 62 21667.0 63 17322.0 64 13320.0 65 10391.5 66 7865.5 67 6495.0 68 4854.5 69 3016.5 70 2248.5 71 1830.0 72 1582.5 73 1381.5 74 1134.0 75 970.5 76 741.5 77 474.5 78 351.0 79 249.0 80 156.5 81 108.5 82 88.0 83 56.5 84 36.5 85 24.0 86 12.0 87 8.5 88 5.5 89 3.5 90 2.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010744455776438816 2 0.010125665129628203 3 0.0050628325648141015 4 1.6876108549380335E-4 5 0.0046690566986618935 6 0.0 7 2.250147806584045E-4 8 0.0018563719404318371 9 1.1250739032920225E-4 10-11 0.0 12-13 0.0 14-15 3.375221709876067E-4 16-17 0.0038533781187751774 18-19 0.0016313571597734325 20-21 5.625369516460113E-4 22-23 3.093953234053062E-4 24-25 4.50029561316809E-4 26-27 5.625369516460113E-5 28-29 2.8126847582300564E-5 30-31 5.062832564814101E-4 32-33 0.00300957269130616 34-35 6.750443419752134E-4 36-37 8.719322750513175E-4 38-39 0.004697183546244194 40-41 5.625369516460113E-5 42-43 6.750443419752134E-4 44-45 0.004725310393826495 46-47 0.0 48-49 0.001406342379115028 50-51 7.03171189557514E-4 52-53 2.250147806584045E-4 54-55 8.438054274690168E-5 56-57 0.011110104795008723 58-59 0.0017157377025203344 60-61 0.002615796825153952 62-63 0.0 64-65 0.002812684758230056 66-67 8.719322750513175E-4 68-69 0.0011813275984566237 70-71 0.003909631813939778 72-73 0.001603230312191132 74-75 0.0060472722301946205 76-77 8.156785798867162E-4 78-79 0.006328540706017627 80-81 0.0020532598735079413 82-83 0.002447035739660149 84-85 0.001546976617026531 86-87 2.250147806584045E-4 88-89 0.0023345283493309468 90-91 0.006778570267334436 92-93 0.0019407524831787386 94-95 3.093953234053062E-4 96-97 1.1250739032920225E-4 98-99 2.8126847582300566E-4 100-101 0.0014907229218619298 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1777661.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.70354677291414 #Duplication Level Percentage of deduplicated Percentage of total 1 77.25119800275276 41.48663325204484 2 13.120972316505103 14.092855010110869 3 4.155721210899078 6.695309052741302 4 1.774883679727003 3.812701948428042 5 0.95282260990851 2.5584976798755896 6 0.5896138213629026 1.899861206011157 7 0.3865682857417669 1.4532061609980753 8 0.277612683452076 1.1927028584418216 9 0.19387908231148918 0.9370794928684254 >10 1.1265101613035715 11.39288921967129 >50 0.10108924085876403 3.763537385800233 >100 0.062195568970622575 6.0337891264854155 >500 0.004832325234754379 1.8114007697806245 >1k 0.001995960423050722 2.282868199488382 >5k 0.0 0.0 >10k+ 1.0505054858161695E-4 0.5866686372539153 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10399 0.5849821760166871 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3739 0.21033256622044358 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3528 0.19846303654071276 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3067 0.17253008306983164 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2845 0.1600417627432902 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2664 0.1498598439184974 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2456 0.13815907532426036 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2396 0.1347838536143843 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2395 0.13472759991921968 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2290 0.1288209619269366 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2272 0.12780839541397376 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1909 0.10738830406922355 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.1250739032920225E-4 0.0 7 0.0 0.0 0.0 1.1250739032920225E-4 0.0 8 0.0 0.0 0.0 1.1250739032920225E-4 0.0 9 0.0 0.0 0.0 2.812684758230056E-4 0.0 10-11 0.0 0.0 0.0 3.093953234053062E-4 0.0 12-13 0.0 0.0 0.0 3.3752217098760676E-4 5.625369516460113E-5 14-15 0.0 0.0 0.0 3.6564901856990734E-4 1.1250739032920225E-4 16-17 0.0 0.0 0.0 5.062832564814102E-4 1.9688793307610393E-4 18-19 0.0 0.0 0.0 7.03171189557514E-4 2.250147806584045E-4 20-21 0.0 0.0 0.0 9.281859702159186E-4 2.250147806584045E-4 22-23 0.0 0.0 0.0 0.001096947055709722 2.5314162824070503E-4 24-25 0.0 0.0 0.0 0.0012094544460389241 3.093953234053062E-4 26-27 0.0 0.0 0.0 0.0015469766170265308 3.3752217098760676E-4 28-29 0.0 0.0 0.0 0.002193894111419444 3.3752217098760676E-4 30-31 0.0 0.0 0.0 0.004331534527674286 3.3752217098760676E-4 32-33 0.0 0.0 0.0 0.00748174145689195 3.3752217098760676E-4 34-35 0.0 0.0 0.0 0.01150388066116093 3.9377586615220786E-4 36-37 0.0 0.0 0.0 0.015582273560594512 3.9377586615220786E-4 38-39 0.0 0.0 0.0 0.020223203411674107 3.9377586615220786E-4 40-41 0.0 0.0 0.0 0.024498484244183792 3.9377586615220786E-4 42-43 0.0 0.0 0.0 0.030180107455808503 4.50029561316809E-4 44-45 0.0 0.0 0.0 0.035974238057762425 4.50029561316809E-4 46-47 0.0 0.0 0.0 0.044187277551794184 4.50029561316809E-4 48-49 0.0 0.0 0.0 0.05383478627252328 4.50029561316809E-4 50-51 0.0 0.0 0.0 0.06058522969227541 5.625369516460112E-4 52-53 0.0 0.0 0.0 0.07394548229386819 7.03171189557514E-4 54-55 0.0 0.0 0.0 0.10395682866418288 7.312980371398146E-4 56-57 0.0 0.0 0.0 0.1603792849142778 7.594248847221151E-4 58-59 0.0 0.0 0.0 0.21798306876282936 7.875517323044157E-4 60-61 0.0 0.0 0.0 0.27190223557809956 7.875517323044157E-4 62-63 0.0 0.0 0.0 0.33372504656399615 7.875517323044157E-4 64-65 0.0 0.0 0.0 0.41312713728883066 7.875517323044157E-4 66-67 0.0 0.0 0.0 0.5515674810889141 7.875517323044157E-4 68-69 0.0 0.0 0.0 0.7196253953931598 7.875517323044157E-4 70-71 0.0 0.0 0.0 0.8626504153491583 7.875517323044157E-4 72-73 0.0 0.0 0.0 1.0058441963906504 7.875517323044157E-4 74-75 0.0 0.0 0.0 1.160119955379569 7.875517323044157E-4 76-77 0.0 0.0 0.0 1.340356794686951 7.875517323044157E-4 78-79 0.0 0.0 0.0 1.4797815781524148 7.875517323044157E-4 80-81 0.0 0.0 0.0 1.5873386433071324 7.875517323044157E-4 82-83 0.0 0.0 0.0 1.7279166275234705 7.875517323044157E-4 84-85 0.0 0.0 0.0 1.9708763369393827 8.156785798867164E-4 86-87 0.0 0.0 0.0 2.3127862961498282 8.719322750513174E-4 88-89 0.0 0.0 0.0 2.7355609421593883 9.00059122633618E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7440 0.0 25.483494 1 ACATGGG 7620 0.0 23.376993 3 TACATGG 7790 0.0 23.359924 2 AATTCGC 480 0.0 22.266586 78-79 ATTCGCG 460 0.0 22.201422 80-81 CGTATAG 110 0.0028716256 21.599133 1 CGCGGAA 465 0.0 20.941177 82-83 CCGTCTA 140 4.8545204E-4 20.356306 9 CTCGCGC 470 0.0 20.211933 60-61 CGATCGT 530 0.0 19.71617 24-25 GTATAGG 605 0.0 19.635574 1 GTGTAGG 1065 0.0 19.631887 1 CAATTCG 560 0.0 19.509771 78-79 CGCGATC 530 0.0 19.268074 22-23 GAGTACT 4395 0.0 19.23698 12-13 GTACTTT 4480 0.0 19.084036 14-15 GTAAGGT 625 0.0 18.999752 4 GAGTGCA 1285 0.0 18.851364 9 TCGCGCT 520 0.0 18.725191 60-61 CCACGTC 640 0.0 18.553925 9 >>END_MODULE