FastQCFastQC Report
Thu 26 May 2016
SRR936550_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936550_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943547
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT78760.4052384634896918No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53040.2729030993333323No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39400.20272213638260358No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38150.19629059652274938No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24570.12641834748529363No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23970.12333120835256364No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22360.11504738501307146No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC19760.1016697821045748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG80750.028.7260381
TACATGG82150.027.6557352
ACATGGG83700.025.8943083
TACCGTA1151.2774418E-424.7961797
AATACCG1252.2451981E-422.8171865
GTATAGG4800.020.7958431
CATGGGA42350.020.650974
CATGGGG42400.020.5145174
GTGTAAG8550.019.4580971
GAGTACT32550.018.32085812-13
GTACCGT1851.513267E-417.9865196
TATGCCG1600.00118541117.8259265
TACCTGG14750.017.7229332
ATACCGT1901.8557916E-417.513196
AGTACAT59550.017.479432
TGCACCG7750.017.1742255
ATGGGAG22200.016.9158925
GTACTTT35000.016.83258214-15
GTGTAGC10750.016.8024861
GCACCGT8900.016.5574136
Produced by FastQC (version 0.11.5)