##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936550_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1943547 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.247332840420118 31.0 31.0 34.0 28.0 34.0 2 31.302529344543764 31.0 31.0 34.0 28.0 34.0 3 31.361774631639985 31.0 31.0 34.0 28.0 34.0 4 34.98802447278095 37.0 35.0 37.0 32.0 37.0 5 34.89843054991724 37.0 35.0 37.0 32.0 37.0 6 35.00861466174988 37.0 35.0 37.0 32.0 37.0 7 34.82782819247489 37.0 35.0 37.0 32.0 37.0 8 34.80858502521421 37.0 35.0 37.0 32.0 37.0 9 36.30387019197374 39.0 37.0 39.0 32.0 39.0 10-11 36.15726401265316 39.0 35.0 39.0 32.0 39.0 12-13 36.066093590738994 39.0 35.0 39.0 30.5 39.0 14-15 37.137525102300074 39.0 36.0 41.0 31.0 41.0 16-17 37.07003175122598 39.0 36.0 41.0 31.0 41.0 18-19 36.98457253670737 39.0 36.0 41.0 31.0 41.0 20-21 36.86038413272229 39.0 36.0 41.0 30.0 41.0 22-23 36.74553329556733 39.0 36.0 40.5 30.0 41.0 24-25 36.58558501543827 39.0 36.0 40.5 30.0 41.0 26-27 36.38960158925923 39.0 35.5 40.0 29.5 41.0 28-29 36.52247848907179 39.0 36.0 40.5 30.0 41.0 30-31 36.49660183159965 39.0 36.0 40.0 30.0 41.0 32-33 36.394067650537906 39.0 35.5 40.0 30.0 41.0 34-35 36.232386970832195 39.0 35.0 40.0 29.5 41.0 36-37 36.003211396482826 38.5 35.0 40.0 28.5 41.0 38-39 35.83041444328334 38.0 35.0 40.0 28.0 41.0 40-41 35.706472753167276 38.0 35.0 40.0 27.5 41.0 42-43 35.47211310042927 38.0 34.5 40.0 27.0 41.0 44-45 35.252827176291596 38.0 34.0 40.0 26.5 41.0 46-47 34.98733321087681 38.0 34.0 40.0 25.5 41.0 48-49 34.81273105306946 38.0 33.5 40.0 25.0 41.0 50-51 34.16325254804747 37.5 33.0 39.5 24.5 40.5 52-53 34.378984660520175 37.5 33.5 39.5 25.0 40.5 54-55 34.7321343399465 38.0 34.0 40.0 25.5 41.0 56-57 34.64653517512054 38.0 34.0 40.0 25.5 41.0 58-59 34.39796670726255 38.0 33.5 40.0 25.0 41.0 60-61 34.05584377429514 37.0 33.0 40.0 23.5 41.0 62-63 33.739268718482236 37.0 33.0 40.0 23.0 41.0 64-65 33.41349192996105 36.0 32.5 39.0 22.5 41.0 66-67 33.02756892424006 36.0 32.0 39.0 22.0 41.0 68-69 32.63126026795339 35.0 31.5 39.0 21.5 40.0 70-71 32.20627800614032 35.0 31.0 38.0 20.0 40.0 72-73 31.778236389446718 35.0 31.0 37.0 19.5 39.5 74-75 31.338103220554995 35.0 31.0 37.0 18.0 39.0 76-77 30.91328431985437 34.5 30.0 36.0 16.5 39.0 78-79 30.450162512149177 34.0 30.0 36.0 12.0 38.0 80-81 30.00713437853574 34.0 29.5 35.0 7.5 37.0 82-83 29.594902515864035 34.0 29.0 35.0 7.0 37.0 84-85 29.204516021480316 34.0 29.0 35.0 4.0 36.0 86-87 28.86484916495459 34.0 29.0 35.0 2.0 36.0 88-89 28.47080878414569 34.0 28.5 35.0 2.0 35.5 90-91 28.09638923061804 34.0 27.0 35.0 2.0 35.0 92-93 27.69942584357363 33.0 27.0 35.0 2.0 35.0 94-95 27.248278791302706 33.0 26.0 35.0 2.0 35.0 96-97 26.641918101285945 33.0 25.0 35.0 2.0 35.0 98-99 25.925333938412606 33.0 24.5 35.0 2.0 35.0 100-101 23.773920054415974 30.5 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22951987854318645 1101 2 0.30605111596022994 1101 3 0.27365908030580144 1101 4 0.3071547075351191 1101 5 0.3644669651197887 1101 6 0.23050028479276818 1101 7 0.3229572791538331 1101 8 0.21242787995028323 1101 9 0.5125923221669879 1101 10-11 0.33218136432132184 1101 12-13 0.5823300714838737 1101 14-15 0.5217159148689277 1101 16-17 0.6484749195799537 1101 18-19 0.6685477171159349 1101 20-21 0.6176191541889793 1101 22-23 0.6167795962466158 1101 24-25 0.6272975501747808 1101 26-27 0.6750638515126042 1101 28-29 0.5571652056123284 1101 30-31 0.29804257620534713 1101 32-33 0.3005254301607323 1101 34-35 0.20311023230037506 1101 36-37 0.44585643671018005 1101 38-39 0.45454224348985406 1101 40-41 0.3964557874089678 1101 42-43 0.2584863544702998 1101 44-45 0.21791371505334212 1101 46-47 0.3151649052104304 1101 48-49 0.1770479943952381 1101 50-51 0.3502629065496805 1101 52-53 0.28096788717346044 1101 54-55 0.4646682846394228 1101 56-57 0.2710536955281384 1101 58-59 0.4030501503421746 1101 60-61 0.42859235439996723 1101 62-63 0.5069995704504535 1101 64-65 0.5964456846382262 1101 66-67 0.4854516532173392 1101 68-69 0.41677116197581654 1101 70-71 0.3609310853687546 1101 72-73 0.25552348952052384 1101 74-75 0.13213331469461664 1101 76-77 0.10715247298884734 1101 78-79 0.19432399037859227 1101 80-81 0.13256360286943902 1101 82-83 0.22142619952094833 1101 84-85 0.1897553024867129 1101 86-87 0.28324686268594945 1101 88-89 0.1541336585232287 1101 90-91 0.23954356575647395 1101 92-93 -0.001141855771084721 1101 94-95 -0.20233678667481314 1101 96-97 -0.11367485331852123 1101 98-99 -0.0916130697414026 1101 100-101 0.18645132971513334 1102 1 0.18150977551158576 1102 2 0.13356117413547253 1102 3 0.24417465236236424 1102 4 0.26515113896278564 1102 5 0.14316909054429772 1102 6 0.2760892947554794 1102 7 -0.03199873478001081 1102 8 -0.02068600228682982 1102 9 0.28640740509757023 1102 10-11 0.34496695937347255 1102 12-13 0.39580239051147714 1102 14-15 0.4596349096571615 1102 16-17 0.2579846204379024 1102 18-19 0.24383105976558994 1102 20-21 -0.006496082940124381 1102 22-23 -0.10209986897361034 1102 24-25 -0.0598032953483596 1102 26-27 0.08774865394910591 1102 28-29 0.06868773002070583 1102 30-31 0.011942602505740751 1102 32-33 -0.022147042528651184 1102 34-35 -0.13245537567961208 1102 36-37 -0.23181249731110398 1102 38-39 -0.21848731646546327 1102 40-41 -0.4096664151071323 1102 42-43 -0.011336129375656867 1102 44-45 -0.05539951343112648 1102 46-47 0.1482227745105078 1102 48-49 0.26852812379624424 1102 50-51 0.2682733469624239 1102 52-53 0.02701075144732812 1102 54-55 -0.06582253590985232 1102 56-57 0.14086713142533824 1102 58-59 0.08103633765158236 1102 60-61 -0.34145651985885195 1102 62-63 0.00683873932734258 1102 64-65 0.20275524870345407 1102 66-67 0.11953937844800322 1102 68-69 0.18553722112640259 1102 70-71 0.2425173978158952 1102 72-73 0.05448861121159965 1102 74-75 -0.09132034868948935 1102 76-77 0.11457294113585093 1102 78-79 -0.10621072038992097 1102 80-81 -0.09141741024011907 1102 82-83 -0.14131105034735825 1102 84-85 -0.3183276595356723 1102 86-87 -0.2725352749762884 1102 88-89 -0.40820325795117185 1102 90-91 -0.5158480148971698 1102 92-93 -0.27465195381864405 1102 94-95 -0.267138885798925 1102 96-97 -0.33507509239906597 1102 98-99 -0.5436294204582985 1102 100-101 -0.13497589985663083 1103 1 0.19842144613773627 1103 2 0.3389408870468529 1103 3 0.2925443472572198 1103 4 0.2633581318633773 1103 5 0.253842756336077 1103 6 0.14441306653696984 1103 7 0.291133304545113 1103 8 0.03538480506778541 1103 9 0.33157493109358427 1103 10-11 0.3307387477813464 1103 12-13 0.46968310107482836 1103 14-15 0.47670997389072767 1103 16-17 0.5252101105322708 1103 18-19 0.3147940273393033 1103 20-21 0.2494414159792271 1103 22-23 0.11972121761981214 1103 24-25 0.19092981459392888 1103 26-27 0.36923520726037395 1103 28-29 0.3159264645069584 1103 30-31 0.06189003990611042 1103 32-33 0.2066679820754942 1103 34-35 0.14470167929326294 1103 36-37 0.2447426813152802 1103 38-39 0.2129115748786603 1103 40-41 0.15638955714535285 1103 42-43 0.2606900205176643 1103 44-45 0.23646555975882677 1103 46-47 0.30524489575720537 1103 48-49 0.39832402875476447 1103 50-51 -0.27208660310310506 1103 52-53 0.07858866685612753 1103 54-55 0.045422575249681074 1103 56-57 0.20188729540682004 1103 58-59 0.21372405010649942 1103 60-61 0.4061020857107138 1103 62-63 0.22618471534912032 1103 64-65 0.12366150399861908 1103 66-67 0.03484529623595023 1103 68-69 0.158372385505551 1103 70-71 0.42823109071890286 1103 72-73 0.41761491014755237 1103 74-75 0.1155627583134411 1103 76-77 0.1706333742931676 1103 78-79 0.3746722275419785 1103 80-81 0.6381194855925685 1103 82-83 0.5345670390021446 1103 84-85 0.361405308625919 1103 86-87 0.27408896122343407 1103 88-89 0.25102770280324194 1103 90-91 0.3713645766118958 1103 92-93 0.21731668844099516 1103 94-95 0.0994515305176158 1103 96-97 0.27396290828562897 1103 98-99 0.2602256312570752 1103 100-101 0.4010882680117156 1104 1 0.289067424555455 1104 2 0.26197793454042184 1104 3 0.2911656534872229 1104 4 0.2878295541608864 1104 5 0.16951735951067803 1104 6 0.2797087641967977 1104 7 0.3039881731065037 1104 8 0.34320132429734684 1104 9 0.5367515027103735 1104 10-11 0.48312215091203115 1104 12-13 0.4837693243322505 1104 14-15 0.4238946506270338 1104 16-17 0.1651886027526075 1104 18-19 0.48494232710717 1104 20-21 0.3701361349076464 1104 22-23 0.34457593717561963 1104 24-25 0.41657504798372 1104 26-27 0.402776658261061 1104 28-29 0.3596986192845435 1104 30-31 0.22003260688019566 1104 32-33 0.1925376453228722 1104 34-35 0.17781170031089033 1104 36-37 0.47993564354628404 1104 38-39 0.375961229028853 1104 40-41 0.20944740660265637 1104 42-43 0.29042790793217677 1104 44-45 0.23391256474660338 1104 46-47 0.33318363877747004 1104 48-49 0.18606694706834048 1104 50-51 0.16287245957334306 1104 52-53 0.32013446254594413 1104 54-55 0.5675125550162861 1104 56-57 0.6055930250636408 1104 58-59 0.6359954833833612 1104 60-61 0.2293660726295883 1104 62-63 0.029593512168240466 1104 64-65 0.37111154791605117 1104 66-67 0.3694884082567782 1104 68-69 0.36880610939036274 1104 70-71 0.484830077480936 1104 72-73 0.11498339311386374 1104 74-75 -0.2042245950336934 1104 76-77 -0.06745176671316955 1104 78-79 0.15369214331461123 1104 80-81 0.29951282504297083 1104 82-83 0.16418611406959016 1104 84-85 0.2765925066935502 1104 86-87 0.08121054585860321 1104 88-89 0.1377376791191942 1104 90-91 -0.12321799208752182 1104 92-93 0.04408711004837684 1104 94-95 0.021522492466292675 1104 96-97 0.2203742264360784 1104 98-99 0.20046213840934257 1104 100-101 0.01445034373791998 1105 1 0.25832108755911776 1105 2 0.30588819095067876 1105 3 0.20535979268136018 1105 4 0.35889182522315366 1105 5 0.36525911775243713 1105 6 0.44853889240192757 1105 7 0.3671520925204206 1105 8 0.23875993235595416 1105 9 0.46815031223417947 1105 10-11 0.4772899622673421 1105 12-13 0.47580801480844315 1105 14-15 0.6580349894548689 1105 16-17 0.6249814141079213 1105 18-19 0.4262655871804313 1105 20-21 0.014057838204344364 1105 22-23 -0.01289887967419645 1105 24-25 0.18840426043793457 1105 26-27 0.15354932309622882 1105 28-29 0.34993442514534934 1105 30-31 0.26260357757616504 1105 32-33 0.19563402810675967 1105 34-35 0.015506297380483147 1105 36-37 -0.051188440702794935 1105 38-39 -0.21985493397480838 1105 40-41 -0.18997452563177575 1105 42-43 -0.13073166166488903 1105 44-45 0.08671132848286334 1105 46-47 0.1046236570924961 1105 48-49 0.2015316906580722 1105 50-51 0.25353180023268607 1105 52-53 0.24176335448733255 1105 54-55 0.251618553184791 1105 56-57 0.46489934374496045 1105 58-59 0.3694089449218225 1105 60-61 0.46425045907647444 1105 62-63 0.35991059092282285 1105 64-65 0.2738359068904117 1105 66-67 -0.07526319430732542 1105 68-69 0.19422964418890132 1105 70-71 0.3821057185065726 1105 72-73 0.3263158106962756 1105 74-75 0.273144910460811 1105 76-77 0.4374646710157677 1105 78-79 0.194002353937325 1105 80-81 0.4060318268744716 1105 82-83 0.4752380829340588 1105 84-85 0.23014104149208237 1105 86-87 0.3744511594117199 1105 88-89 0.3394833293023467 1105 90-91 0.38398782293079137 1105 92-93 0.3036796008908631 1105 94-95 0.2318990950303963 1105 96-97 0.3098430909049412 1105 98-99 0.10416520617491187 1105 100-101 0.3534269920895703 1106 1 0.035774099028522244 1106 2 0.047955686296862865 1106 3 0.03255909611017316 1106 4 0.2460262754723601 1106 5 0.4040899590422953 1106 6 0.36810893984082327 1106 7 0.4694594845819111 1106 8 0.4251978114167798 1106 9 0.6486147931085 1106 10-11 0.4750974143780766 1106 12-13 0.4267252669552022 1106 14-15 0.4110982513296122 1106 16-17 0.32917059923973113 1106 18-19 0.3888211327277773 1106 20-21 0.377798605633636 1106 22-23 0.23079519947070537 1106 24-25 0.1360495795996428 1106 26-27 0.16699284678566073 1106 28-29 -0.034468535282201174 1106 30-31 0.0868724780745751 1106 32-33 0.11065459497158514 1106 34-35 0.18714278930386286 1106 36-37 0.29676087773832194 1106 38-39 0.3289801107618757 1106 40-41 0.16172711971741194 1106 42-43 0.22659959996965284 1106 44-45 0.11788138793160385 1106 46-47 0.13976297133016402 1106 48-49 0.19997941779433148 1106 50-51 0.289541077840326 1106 52-53 0.1994999484944202 1106 54-55 0.3132861205900568 1106 56-57 0.271540039115159 1106 58-59 0.3807042070367572 1106 60-61 0.3425576709902458 1106 62-63 0.37650144542350716 1106 64-65 0.22742009592541734 1106 66-67 0.33186765298746224 1106 68-69 0.4248292358540695 1106 70-71 0.35559998380411884 1106 72-73 0.5615729715682001 1106 74-75 0.4914794447789532 1106 76-77 0.44080716185825963 1106 78-79 0.4913714465933019 1106 80-81 0.5305176552537425 1106 82-83 0.6721230285894997 1106 84-85 0.677251171798936 1106 86-87 0.8952071793480698 1106 88-89 0.976595261086409 1106 90-91 0.7939695079124789 1106 92-93 0.7587387493926379 1106 94-95 0.6365656716231989 1106 96-97 0.8731845308857302 1106 98-99 0.9257731747091107 1106 100-101 0.9342322524030244 1107 1 0.51979477647183 1107 2 0.5500005826240439 1107 3 0.5592841203861774 1107 4 0.39845673186123065 1107 5 0.5902926208347168 1107 6 0.5870585028727575 1107 7 0.6625465354758404 1107 8 0.822600279001179 1107 9 0.8052292901668139 1107 10-11 0.8720757658946141 1107 12-13 0.8204799079559493 1107 14-15 0.9549214363413228 1107 16-17 1.026256764773521 1107 18-19 1.234136577978255 1107 20-21 1.1342367446637454 1107 22-23 1.131128637524057 1107 24-25 1.0650179922345941 1107 26-27 1.0012577122680355 1107 28-29 1.0355805879256579 1107 30-31 0.916602728831414 1107 32-33 1.1465609467165976 1107 34-35 0.8654750278370216 1107 36-37 0.9242191801316508 1107 38-39 1.1910145826873944 1107 40-41 1.1896902201915083 1107 42-43 1.41072951943044 1107 44-45 1.2373914410532194 1107 46-47 1.227859161425556 1107 48-49 1.1765395080439447 1107 50-51 1.0057296024304918 1107 52-53 1.0551355513961127 1107 54-55 1.307457023657399 1107 56-57 1.2679079205340216 1107 58-59 1.3337415635227359 1107 60-61 1.2102948618281957 1107 62-63 1.1281943396943817 1107 64-65 1.0779713040136158 1107 66-67 1.037013451810786 1107 68-69 1.2373056738502939 1107 70-71 1.1747037708607238 1107 72-73 1.1549441945075962 1107 74-75 1.0469622516562005 1107 76-77 1.196120750708431 1107 78-79 1.097578249586391 1107 80-81 1.1435731473426216 1107 82-83 1.1798927778326558 1107 84-85 1.3520498237667198 1107 86-87 1.3452186033498954 1107 88-89 1.256024917795152 1107 90-91 1.389352783173802 1107 92-93 1.3509468661459358 1107 94-95 1.3495440516997412 1107 96-97 1.307003146296708 1107 98-99 1.7843961540887854 1107 100-101 2.035606702622939 1108 1 0.09089613762708737 1108 2 0.11445162940080067 1108 3 0.003620125360956905 1108 4 -0.0742303406185556 1108 5 0.15123022879436832 1108 6 0.27088010171197396 1108 7 0.34590693069452527 1108 8 0.13699432308326465 1108 9 0.5711416787524612 1108 10-11 0.19339822841102006 1108 12-13 0.2511654949109712 1108 14-15 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2316 94-95 -4.000499129851793 2316 96-97 -4.3120034172991275 2316 98-99 -4.854820679220648 2316 100-101 -5.388552968089218 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5468.0 3 2177.0 4 2148.0 5 2342.0 6 2575.0 7 2982.0 8 3481.0 9 4544.0 10 5409.0 11 6443.0 12 7308.0 13 8232.0 14 9200.0 15 10319.0 16 11686.0 17 13210.0 18 12604.0 19 11521.0 20 12565.0 21 13838.0 22 15700.0 23 18095.0 24 20859.0 25 24046.0 26 28343.0 27 33800.0 28 39943.0 29 47561.0 30 58165.0 31 71678.0 32 88517.0 33 113127.0 34 148706.0 35 199032.0 36 272241.0 37 338625.0 38 246124.0 39 30929.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.996500725584845 17.861325813324 13.517954365344833 23.624219095746323 2 15.799467016843153 19.47415896153187 39.570062997806424 25.15631102381855 3 20.51650828952169 23.018930644240868 28.826715832448695 27.63784523378875 4 12.885584712442116 15.40094736576626 36.67333895734178 35.04012896444985 5 14.368834682627234 37.06667435387551 33.70904566562392 14.855445297873338 6 33.56957406136527 34.43521802499937 17.34505647485107 14.65015143878429 7 30.54545248681311 31.372769011682276 19.838664271094256 18.243114230410352 8 27.385390060044866 33.29981209924412 19.33252257922737 19.98227526148364 9 26.38449405765069 16.89701042837919 20.029023468355927 36.68947204561419 10-11 25.7633758228182 25.368423084595044 27.13442420420691 21.733776888379843 12-13 26.911357565297333 22.566729611400984 27.375863452564573 23.146049370737106 14-15 23.259285867202422 24.499425680126475 25.650741469600156 26.590546983070944 16-17 23.212894808959362 27.977608032494345 25.99454739380091 22.81494976474539 18-19 22.975755021078676 27.008238465145173 27.537019566547727 22.478986947228428 20-21 23.780717198234356 25.752037091455925 27.21355017706343 23.25369553324629 22-23 23.25739841824702 25.78800763860408 27.403903798049388 23.550690145099516 24-25 23.649442704736934 25.924186027844698 27.374872266135114 23.051499001283258 26-27 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26.44277621416921 27.21781724817469 23.495325467839116 52-53 23.505014072332305 26.571007393762898 26.566222286252334 23.357756247652468 54-55 22.87422983722935 26.055369651116813 27.032214793762698 24.038185717891135 56-57 23.41789704683034 26.269369000851555 26.728668050763954 23.58406590155415 58-59 23.94116934267021 25.90764539730735 26.592629239406808 23.558556020615633 60-61 23.381344010833917 26.038241834686026 27.519467195190593 23.060946959289456 62-63 23.085052685523586 26.278445268953693 27.295829768054038 23.340672277468688 64-65 22.87805543192185 26.651856349355402 26.919445423509043 23.5506427952137 66-67 23.04671792630272 26.504054917289878 26.477968021170007 23.971259135237403 68-69 22.845484424948385 26.252326770564665 27.080354788291217 23.82183401619574 70-71 23.1851963915677 26.19838967713783 26.891089913895534 23.72532401739894 72-73 23.15785384367411 25.828804651941795 27.42614731097563 23.58719419340846 74-75 22.736230398603745 26.795461140920118 26.88416781409057 23.584140646385563 76-77 22.986475093626744 26.267713958688205 26.806178683331545 23.939632264353506 78-79 23.1126283728226 26.242808484026504 27.00427598435785 23.640287158793047 80-81 23.21607455732361 26.570515962802656 26.75470660088503 23.458702878988706 82-83 23.129333432812015 26.36911392795857 27.07797670655247 23.423575932676947 84-85 22.984407839844025 26.50731547424707 26.75080870678696 23.757467979121945 86-87 23.124620992945097 26.418280740382645 26.82674003634351 23.630358230328746 88-89 22.93830466333614 26.3873230776197 26.43479052832688 24.23958173071728 90-91 23.021068516095667 26.085851276371024 26.838899244970865 24.05418096256244 92-93 23.329476413481363 26.057623257406814 27.049898868422428 23.563001460689392 94-95 22.933814821666047 25.880169402524466 27.172171099534147 24.013844676275337 96-97 22.552233998433728 25.909483366315644 27.5093827213854 24.028899913865228 98-99 22.657147812428754 26.313821637792568 26.718537628963336 24.310492920815342 100-101 22.361500322235926 25.93283697332957 27.20389298061025 24.501769723824253 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 42.0 1 35.0 2 38.5 3 57.0 4 67.5 5 73.0 6 111.0 7 217.0 8 342.0 9 434.5 10 440.0 11 432.0 12 480.0 13 570.0 14 644.5 15 686.0 16 738.0 17 806.5 18 900.5 19 1000.5 20 1140.5 21 1310.5 22 1600.0 23 1987.0 24 2509.5 25 3298.5 26 4421.0 27 5961.5 28 7853.0 29 10310.0 30 13456.0 31 17337.5 32 22264.5 33 26289.0 34 30527.5 35 34948.5 36 39426.5 37 44108.0 38 47923.0 39 50658.0 40 53240.0 41 58101.0 42 63235.5 43 67536.0 44 70472.5 45 74223.0 46 76249.5 47 82507.5 48 99779.0 49 101048.5 50 86400.5 51 78182.0 52 73615.0 53 71396.5 54 69643.0 55 64920.5 56 58880.0 57 52891.5 58 48209.5 59 42793.5 60 36168.0 61 30064.0 62 24792.5 63 19562.5 64 14932.5 65 11834.5 66 8823.5 67 7058.5 68 5396.0 69 3537.5 70 2667.0 71 2132.5 72 1733.0 73 1543.0 74 1271.0 75 1017.5 76 757.5 77 489.5 78 314.0 79 210.5 80 142.0 81 103.5 82 73.5 83 48.0 84 34.0 85 20.5 86 17.5 87 14.5 88 10.0 89 6.0 90 3.0 91 4.5 92 5.0 93 3.0 94 2.0 95 2.0 96 1.0 97 1.5 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014766815518225184 2 0.007512038556309675 3 0.009775940586978345 4 0.03529629075087971 5 0.04764484728179972 6 0.060610831639265735 7 0.0266523011792357 8 0.025726159439416696 9 0.01502407711261935 10-11 0.04679588402029897 12-13 0.04309131706102297 14-15 0.019500428855077856 16-17 0.022613294147247276 18-19 0.030253963500754037 20-21 0.023565162046505692 22-23 0.01337760290849668 24-25 0.0027269729005781696 26-27 0.002469711306184003 28-29 0.001337760290849668 30-31 0.006714527613687757 32-33 0.0015178434069255849 34-35 0.001672200363562085 36-37 0.006431539859854174 38-39 0.00676597993256659 40-41 0.004064733191427837 42-43 8.48963261500751E-4 44-45 0.001852283479638002 46-47 0.004039007031988421 48-49 5.402493482277506E-4 50-51 0.0019037357985168355 52-53 8.746894209401677E-4 54-55 0.002341080508986919 56-57 0.0021609973929110023 58-59 0.0036273884809577537 60-61 0.0010804986964555012 62-63 0.0017493788418803354 64-65 0.0042190901480643386 66-67 0.002186723552350419 68-69 0.006920336889203091 70-71 0.010804986964555011 72-73 0.005170958047322756 74-75 0.0032414960893665037 76-77 0.0030099606544117534 78-79 0.03701994343332063 80-81 0.023230721973793274 82-83 0.0042190901480643386 84-85 0.00856681109332576 86-87 0.007537764715749092 88-89 0.005659755076671673 90-91 0.012580091965874764 92-93 0.014741089358785766 94-95 0.00661162297593009 96-97 0.004167637829185505 98-99 0.023462257408748025 100-101 0.044814969743463884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1943547.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.49915087129788 #Duplication Level Percentage of deduplicated Percentage of total 1 79.249939908693 44.77554311442536 2 11.992322827615535 13.55112113469519 3 3.799356756045 6.43981291721014 4 1.6363877721996298 3.698180785018156 5 0.8732286467508805 2.4668338528958635 6 0.530926752299837 1.7998146407878013 7 0.34953063707895765 1.3823728938925424 8 0.2244478423512857 1.0144890006194058 9 0.16674991754998977 0.8479105874490019 >10 1.0226639513924594 10.788202050472409 >50 0.09168833543096791 3.629245685517367 >100 0.0574442009057328 5.839758519425473 >500 0.0033889777999742137 1.3074594346029498 >1k 0.0017402858972840557 1.7771926342028301 >5k 1.8318798918779533E-4 0.6820627487855484 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7876 0.4052384634896918 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5304 0.2729030993333323 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3940 0.20272213638260358 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3815 0.19629059652274938 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2457 0.12641834748529363 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2397 0.12333120835256364 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2236 0.11504738501307146 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1976 0.1016697821045748 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 5.145231887883339E-5 3 0.0 0.0 0.0 0.0 5.145231887883339E-5 4 0.0 0.0 0.0 5.145231887883339E-5 5.145231887883339E-5 5 0.0 0.0 0.0 5.145231887883339E-5 5.145231887883339E-5 6 0.0 0.0 0.0 5.145231887883339E-5 5.145231887883339E-5 7 0.0 0.0 0.0 5.145231887883339E-5 5.145231887883339E-5 8 0.0 0.0 0.0 5.145231887883339E-5 5.145231887883339E-5 9 0.0 0.0 0.0 5.145231887883339E-5 1.0290463775766678E-4 10-11 0.0 0.0 0.0 7.717847831825008E-5 1.0290463775766678E-4 12-13 0.0 0.0 0.0 1.0290463775766678E-4 1.0290463775766678E-4 14-15 0.0 0.0 0.0 1.2863079719708349E-4 1.8008311607591688E-4 16-17 0.0 0.0 0.0 2.0580927551533355E-4 2.0580927551533355E-4 18-19 0.0 0.0 0.0 2.0580927551533355E-4 2.0580927551533355E-4 20-21 0.0 0.0 0.0 2.3153543495475027E-4 2.8298775383358367E-4 22-23 0.0 0.0 0.0 2.5726159439416697E-4 3.3444007271241706E-4 24-25 0.0 0.0 0.0 3.0871391327300037E-4 3.6016623215183376E-4 26-27 0.0 0.0 0.0 5.145231887883339E-4 4.116185510306671E-4 28-29 0.0 0.0 0.0 0.001260581812531418 4.116185510306671E-4 30-31 0.0 0.0 0.0 0.0027269729005781696 4.116185510306671E-4 32-33 0.0 0.0 0.0 0.005299588844519839 4.116185510306671E-4 34-35 0.0 0.0 0.0 0.008335275658371008 4.116185510306671E-4 36-37 0.0 0.0 0.0 0.011859759501571096 4.116185510306671E-4 38-39 0.0 0.0 0.0 0.0160273973307566 4.116185510306671E-4 40-41 0.0 0.0 0.0 0.019731964290032607 4.116185510306671E-4 42-43 0.0 0.0 0.0 0.024465577626885275 4.373447104700838E-4 44-45 0.0 0.0 0.0 0.029482178717571535 4.630708699095005E-4 46-47 0.0 0.0 0.0 0.03619670633125929 4.630708699095005E-4 48-49 0.0 0.0 0.0 0.04507223133785805 4.630708699095005E-4 50-51 0.0 0.0 0.0 0.05232700829977356 4.630708699095005E-4 52-53 0.0 0.0 0.0 0.06521581417892132 4.630708699095005E-4 54-55 0.0 0.0 0.0 0.08785483448560802 5.145231887883339E-4 56-57 0.0 0.0 0.0 0.13454781386814932 5.402493482277507E-4 58-59 0.0 0.0 0.0 0.18059763926470518 5.659755076671673E-4 60-61 0.0 0.0 0.0 0.2257213229214421 5.659755076671673E-4 62-63 0.0 0.0 0.0 0.2828591230363866 5.659755076671673E-4 64-65 0.0 5.145231887883339E-5 0.0 0.35167659953682623 5.659755076671673E-4 66-67 0.0 5.145231887883339E-5 0.0 0.4704028253497343 5.659755076671673E-4 68-69 0.0 5.145231887883339E-5 0.0 0.6209780365486401 5.659755076671673E-4 70-71 0.0 5.145231887883339E-5 0.0 0.7418395335950199 5.91701667106584E-4 72-73 0.0 5.145231887883339E-5 0.0 0.8661483360062814 6.431539859854175E-4 74-75 0.0 5.145231887883339E-5 0.0 1.0060729171972687 6.946063048642508E-4 76-77 0.0 5.145231887883339E-5 0.0 1.1647518686195908 7.203324643036675E-4 78-79 0.0 5.145231887883339E-5 0.0 1.279541992038268 7.203324643036675E-4 80-81 0.0 5.145231887883339E-5 0.0 1.3786648843583407 7.203324643036675E-4 82-83 0.0 5.145231887883339E-5 0.0 1.5103570945287148 8.232371020613342E-4 84-85 0.0 5.145231887883339E-5 0.0 1.7255049659205566 8.232371020613342E-4 86-87 0.0 5.145231887883339E-5 0.0 2.047545029783175 8.489632615007508E-4 88-89 0.0 5.145231887883339E-5 0.0 2.444808383846647 8.746894209401676E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8075 0.0 28.726038 1 TACATGG 8215 0.0 27.655735 2 ACATGGG 8370 0.0 25.894308 3 TACCGTA 115 1.2774418E-4 24.796179 7 AATACCG 125 2.2451981E-4 22.817186 5 GTATAGG 480 0.0 20.795843 1 CATGGGA 4235 0.0 20.65097 4 CATGGGG 4240 0.0 20.514517 4 GTGTAAG 855 0.0 19.458097 1 GAGTACT 3255 0.0 18.320858 12-13 GTACCGT 185 1.513267E-4 17.986519 6 TATGCCG 160 0.001185411 17.825926 5 TACCTGG 1475 0.0 17.722933 2 ATACCGT 190 1.8557916E-4 17.51319 6 AGTACAT 5955 0.0 17.47943 2 TGCACCG 775 0.0 17.174225 5 ATGGGAG 2220 0.0 16.915892 5 GTACTTT 3500 0.0 16.832582 14-15 GTGTAGC 1075 0.0 16.802486 1 GCACCGT 890 0.0 16.557413 6 >>END_MODULE