FastQCFastQC Report
Thu 26 May 2016
SRR936550_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936550_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943547
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103890.5345381408322001No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38000.19551881173956687No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT37860.19479847927526323No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30370.156260692435017No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT28980.14910882011085916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28740.14787396445776715No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25910.13331295821505731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25440.13089469922775215No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24500.1260581812531418No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23660.1217361864673198No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG21640.11134281805379546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG81850.026.5315191
TACATGG85650.024.7345712
ACATGGG85800.024.2471873
GTAACGC1100.002878806621.5899623
CCACGTC6350.020.9401919
ATTCGCG4100.020.8516780-81
GAGTACT45550.019.70419112-13
TAAGGTG7850.019.358725
CATGGGG39950.019.0229454
CGCGGAA4500.019.00112282-83
AATTCGC4650.018.89750778-79
TCTATAC5050.018.8110563
TCGCGGA4700.018.69791282-83
GTGTTAC5950.018.5717681
GTACTTT48050.018.53075214-15
GCACCGT5700.018.3292276
AGTACTT47200.018.31110612-13
ACCGTAC1300.00756687318.2684293
CATGGGA45450.017.7659974
CAATTCG4950.017.75220378-79
Produced by FastQC (version 0.11.5)