FastQCFastQC Report
Thu 26 May 2016
SRR936549_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936549_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2035406
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT82000.40286802731248705No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54360.267072023959839No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT43470.21356918472285139No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC39390.19352404385169347No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26370.12955646195402784No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25240.12400474401667283No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22650.11128000998326625No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20960.10297699820085034No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20530.10086439756982145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG86400.029.5907141
TACATGG88150.028.4648692
ACATGGG90100.027.11043
CATGGGG43950.022.9248544
CATGGGA47650.019.548924
GATGTGC11850.019.2471778
GTGTAGC10250.019.008461
TACCTGG16700.018.4967082
CGCGAAA650.007576423718.26501572-73
GTATAGT5750.018.1820031
GAGTACT34900.017.90641212-13
TACGGCA1350.00937344217.6021564
ATGGGAG21650.017.3398535
GTATTAG6600.017.2804161
GTGTAGG10200.016.7721671
GTACCTG19100.016.6697221
CATGGGC20850.016.6397344
AGTACAT62450.016.5890522
AGAGTAC86200.016.53786810-11
GAGTACA72850.016.5035691
Produced by FastQC (version 0.11.5)