FastQCFastQC Report
Thu 26 May 2016
SRR936549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936549_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2035406
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT108000.5306066701188853No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40880.20084445068944476No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38750.19037970802876675No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32760.16095068993606187No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30620.15043681702815065No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29420.14454118736016303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28350.1392842509062074No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC26520.1302934156625263No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC25350.12454517673623838No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24300.1193865007767492No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23370.11481738778405881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG85550.027.3838711
TACATGG90700.025.8175772
ACATGGG90050.024.790773
CGCGGAA4250.024.02931482-83
ATTCGCG4850.022.52485380-81
TCGCGCT5000.021.85071860-61
CAATTCG5250.021.71339678-79
AATTCGC5050.021.6327878-79
CTCGCGC5250.021.26260260-61
CATGGGA46300.020.9273574
CGATCGT5200.020.5525524-25
CGCGCTT5450.020.48081462-63
TAAGGTG8600.020.4342085
TCGCGGA5200.020.09607382-83
GAGTACT47250.019.90590512-13
CGCGATC5550.019.25644322-23
GCGCCCT5800.019.24608654-55
CCGCGAT6250.018.61969622-23
GTACTTT50000.018.52606214-15
CCCGCGA5950.018.36059420-21
Produced by FastQC (version 0.11.5)