FastQCFastQC Report
Thu 26 May 2016
SRR936548_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936548_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123955
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT82340.38767299683844525No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55520.26139913510408647No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41200.19397774434957427No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC40460.19049367806756734No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26340.12401392684873266No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25440.11977654893818371No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23260.10951267799929848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG86600.028.1573941
TACATGG89250.026.5206032
ACATGGG91300.024.7833423
ACCGTCG1852.5200097E-723.1305858
CATGGGG43550.021.939234
ATACCGT2308.961797E-820.66696
GTGTAGC13000.020.110011
TACGAAC1200.00474390119.8067153
GAGTACT35800.019.05405612-13
CATGGGA46550.018.585114
TAGCCCT13650.018.456814
TACCGTC2854.242429E-818.3464077
GTAGCCC11000.017.7180083
TAAGGTG7950.017.3402185
GTACTTT39400.017.00784714-15
AGTACAT65900.016.7326552
ATGGGGA27850.016.7272515
GAGTACA76900.016.565381
AGTACTT39600.016.44538112-13
CTCGCGC4350.016.37459260-61
Produced by FastQC (version 0.11.5)