##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936548_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2123955 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23303506901041 31.0 31.0 34.0 28.0 34.0 2 31.296995934471305 31.0 31.0 34.0 28.0 34.0 3 31.24717049090023 31.0 31.0 34.0 27.0 34.0 4 34.90117540155041 37.0 35.0 37.0 32.0 37.0 5 34.93241570560581 37.0 35.0 37.0 32.0 37.0 6 34.98439044141708 37.0 35.0 37.0 32.0 37.0 7 34.84417183979887 37.0 35.0 37.0 32.0 37.0 8 34.8218653408382 37.0 35.0 37.0 32.0 37.0 9 36.293762815125554 39.0 37.0 39.0 32.0 39.0 10-11 36.14900080274771 39.0 35.0 39.0 31.5 39.0 12-13 36.051777697738416 39.0 35.0 39.0 30.5 39.0 14-15 37.02700010122625 39.0 36.0 41.0 31.0 41.0 16-17 36.93250704464077 39.0 36.0 41.0 31.0 41.0 18-19 36.76314893677126 39.0 36.0 41.0 30.0 41.0 20-21 36.723011080743234 39.0 36.0 41.0 30.0 41.0 22-23 36.72081188160766 39.0 36.0 40.5 30.0 41.0 24-25 36.84134315463369 39.0 36.0 41.0 30.0 41.0 26-27 36.768515575894966 39.0 36.0 41.0 30.0 41.0 28-29 36.65576365789294 39.0 36.0 41.0 30.0 41.0 30-31 36.49892912043805 39.0 36.0 41.0 30.0 41.0 32-33 36.37069264650145 39.0 35.0 40.0 30.0 41.0 34-35 36.182239501307706 39.0 35.0 40.0 29.5 41.0 36-37 35.98548933475521 38.5 35.0 40.0 29.0 41.0 38-39 35.800081451819835 38.0 35.0 40.0 28.0 41.0 40-41 35.599997410491284 38.0 35.0 40.0 27.0 41.0 42-43 35.34887768337841 38.0 34.0 40.0 26.5 41.0 44-45 35.26325981482658 38.0 34.0 40.0 26.0 41.0 46-47 35.21275474292064 38.0 34.0 40.0 26.0 41.0 48-49 35.039176912881864 38.0 34.0 40.0 26.0 41.0 50-51 34.081882619923675 37.5 33.0 39.5 24.0 40.5 52-53 34.20696719092448 37.5 33.0 39.5 24.0 40.5 54-55 34.65282362385267 38.0 34.0 40.0 25.0 41.0 56-57 34.48030019468398 38.0 33.5 40.0 24.5 41.0 58-59 34.245092292444994 38.0 33.0 40.0 24.0 41.0 60-61 33.928043908651546 37.0 33.0 40.0 23.0 41.0 62-63 33.59051274626816 37.0 33.0 40.0 22.5 41.0 64-65 33.21774943442776 36.0 32.0 39.0 22.0 41.0 66-67 32.83053383899376 36.0 32.0 39.0 21.5 40.5 68-69 32.379227667252835 35.0 31.0 39.0 20.0 40.0 70-71 31.955075554802246 35.0 31.0 38.0 19.5 40.0 72-73 31.529435887295165 35.0 31.0 37.0 18.0 39.5 74-75 31.102653775621423 35.0 30.5 37.0 16.5 39.0 76-77 30.629720262434937 34.0 30.0 36.0 12.5 39.0 78-79 30.15093281166503 34.0 29.5 36.0 8.0 37.5 80-81 29.67084778161496 34.0 29.0 35.0 7.0 37.0 82-83 29.266004694073086 34.0 29.0 35.0 4.5 37.0 84-85 28.889063327612874 34.0 29.0 35.0 2.0 36.0 86-87 28.53938689849832 34.0 28.5 35.0 2.0 36.0 88-89 28.16307030987003 34.0 27.0 35.0 2.0 35.5 90-91 27.782740453540683 33.0 27.0 35.0 2.0 35.0 92-93 27.320914049497283 33.0 26.0 35.0 2.0 35.0 94-95 26.802156119126817 33.0 25.0 35.0 2.0 35.0 96-97 26.1626213832214 33.0 24.5 35.0 2.0 35.0 98-99 25.36845201522631 32.0 21.0 35.0 2.0 35.0 100-101 22.729158809861794 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5063268589303576 1101 2 0.4860955750457663 1101 3 0.5129349839879325 1101 4 0.654263391875844 1101 5 0.5683034947195225 1101 6 0.5874194361556775 1101 7 0.5917635178611604 1101 8 0.5417309543992204 1101 9 0.7123924465577716 1101 10-11 0.7835970171268727 1101 12-13 0.8399411403692483 1101 14-15 1.001816911164589 1101 16-17 1.0344648380744346 1101 18-19 1.1859986907284181 1101 20-21 1.0333353411785993 1101 22-23 0.9162599433413092 1101 24-25 0.7014836262653503 1101 26-27 0.7323304239239121 1101 28-29 0.8096310188326967 1101 30-31 0.6441327682994924 1101 32-33 0.5927095181329562 1101 34-35 0.6940333399834273 1101 36-37 0.6549722367785122 1101 38-39 0.7265255344564849 1101 40-41 0.7061282276651681 1101 42-43 0.6980057014299064 1101 44-45 0.7093521641203964 1101 46-47 0.8110811068638526 1101 48-49 0.7271416887692155 1101 50-51 0.7185062055131866 1101 52-53 0.8568103990657718 1101 54-55 0.808543530239497 1101 56-57 0.8846053052268985 1101 58-59 0.7992313317392501 1101 60-61 0.7479834336762252 1101 62-63 0.8660464398357135 1101 64-65 0.8157147463417118 1101 66-67 0.7560707728951996 1101 68-69 0.8094216828505054 1101 70-71 0.7748298399270404 1101 72-73 1.0341690516449127 1101 74-75 0.9134494867598413 1101 76-77 0.8255306034961905 1101 78-79 0.893657289775664 1101 80-81 0.9128367040473613 1101 82-83 0.934251808127545 1101 84-85 0.9017825347280279 1101 86-87 0.9128117312349673 1101 88-89 0.9534306433011999 1101 90-91 0.8586494574860346 1101 92-93 0.7907867770581625 1101 94-95 0.9249826463512676 1101 96-97 0.7270505426631004 1101 98-99 0.7657678328063398 1101 100-101 1.1930053596740322 1102 1 0.28017790196410886 1102 2 0.438188162312354 1102 3 0.44701796167271013 1102 4 0.3311221950821732 1102 5 0.3659478211837808 1102 6 0.4079529108301614 1102 7 0.43850975136569303 1102 8 0.383099208692812 1102 9 0.5315182030800187 1102 10-11 0.443123439803756 1102 12-13 0.41465295759839904 1102 14-15 0.6339613333132732 1102 16-17 0.3798997724782609 1102 18-19 0.4875010648409486 1102 20-21 0.5971887203082318 1102 22-23 0.24519473137183212 1102 24-25 0.3762458201532226 1102 26-27 0.43855176680438746 1102 28-29 0.1215693319437463 1102 30-31 0.15904089839064994 1102 32-33 0.34074532431192495 1102 34-35 0.06439719058936078 1102 36-37 0.17469920740945355 1102 38-39 0.02098077783469421 1102 40-41 0.2767841086212499 1102 42-43 0.05041926312762968 1102 44-45 0.20135443336572223 1102 46-47 0.17630370998724487 1102 48-49 0.4493726346199125 1102 50-51 0.30468573741980975 1102 52-53 0.12580348297311872 1102 54-55 0.3646611629515988 1102 56-57 0.345721613784022 1102 58-59 0.14244651592167656 1102 60-61 0.3206072733877221 1102 62-63 0.2295608646767846 1102 64-65 0.40616169364108146 1102 66-67 0.3673045657709082 1102 68-69 0.2967014387799125 1102 70-71 0.07181107879982207 1102 72-73 0.3498539336151367 1102 74-75 0.28673566107593373 1102 76-77 0.3031127148787469 1102 78-79 0.03662571464832354 1102 80-81 0.4134983223961477 1102 82-83 0.22520538703745174 1102 84-85 0.2835600135149221 1102 86-87 0.1365919935021367 1102 88-89 0.07678963695811092 1102 90-91 0.3429555580388026 1102 92-93 0.20574847941089658 1102 94-95 0.4573057100455067 1102 96-97 0.4424721914399008 1102 98-99 0.6442172014176606 1102 100-101 1.3668010673549773 1103 1 0.2827348838927506 1103 2 0.4979565109601829 1103 3 0.486263768254787 1103 4 0.42590877801926297 1103 5 0.5149906516674321 1103 6 0.44428018577267636 1103 7 0.5860043704750595 1103 8 0.3938718040177491 1103 9 0.5515195079752857 1103 10-11 0.40603990235524634 1103 12-13 0.3971017557790404 1103 14-15 0.7033057928613431 1103 16-17 0.8864051439967184 1103 18-19 0.9626379436684473 1103 20-21 0.8882850810562601 1103 22-23 0.7079193346674444 1103 24-25 0.5765651770489271 1103 26-27 0.5760756523355184 1103 28-29 0.6096323338733285 1103 30-31 0.5768079846784673 1103 32-33 0.6280402764219062 1103 34-35 0.6724390943089986 1103 36-37 0.6135762188676566 1103 38-39 0.5129178227659992 1103 40-41 0.624762097705549 1103 42-43 0.7319784976227197 1103 44-45 0.6133070757606234 1103 46-47 0.7776682988064536 1103 48-49 0.9641456170878158 1103 50-51 0.9041767922185642 1103 52-53 1.0031263857955466 1103 54-55 0.9729352983773794 1103 56-57 0.9819071706120326 1103 58-59 0.7531229022557469 1103 60-61 0.8145564524031315 1103 62-63 0.9365839407763303 1103 64-65 1.0587050318443403 1103 66-67 0.9741361070360597 1103 68-69 1.0373147539744778 1103 70-71 0.9107902469759459 1103 72-73 0.8900720133355868 1103 74-75 0.9842392197992886 1103 76-77 0.8931943753687861 1103 78-79 0.8584171231746254 1103 80-81 0.8788562367842019 1103 82-83 0.8771487257453678 1103 84-85 0.9709419826739705 1103 86-87 0.7648716693257782 1103 88-89 0.8221503197427573 1103 90-91 1.0830415982187631 1103 92-93 0.9752024131834993 1103 94-95 1.057569608892333 1103 96-97 1.0304855749071642 1103 98-99 1.4938415393725606 1103 100-101 1.9652436099686383 1104 1 0.31040673074103253 1104 2 0.354831622815464 1104 3 0.386142593174835 1104 4 0.38487407158056897 1104 5 0.3913241611396714 1104 6 0.2807511260361153 1104 7 0.27307061072994543 1104 8 0.3499400016074077 1104 9 0.503208283965499 1104 10-11 0.5102428667235444 1104 12-13 0.5499264862476068 1104 14-15 0.5395786652766432 1104 16-17 0.5446371267195431 1104 18-19 0.43132854541978816 1104 20-21 0.5785405673588073 1104 22-23 0.40519402718479824 1104 24-25 0.6116723494394023 1104 26-27 0.2826303540771704 1104 28-29 0.10555867951445919 1104 30-31 0.17605341691556475 1104 32-33 0.2709419845448835 1104 34-35 0.3012480981904204 1104 36-37 0.4676186068019277 1104 38-39 0.16831991110849742 1104 40-41 0.3711411698567204 1104 42-43 0.4776871454771765 1104 44-45 0.6130389767804516 1104 46-47 0.6024344903225014 1104 48-49 0.627356707189584 1104 50-51 0.4673489407666196 1104 52-53 0.4566193476667806 1104 54-55 0.6246206081802512 1104 56-57 0.8195074513024707 1104 58-59 0.5602150752510369 1104 60-61 0.6751750465882864 1104 62-63 0.5484183532257418 1104 64-65 0.6160078287745705 1104 66-67 0.6417446570591565 1104 68-69 0.3262396841378816 1104 70-71 0.4053884769567695 1104 72-73 0.5313101283804649 1104 74-75 0.43060987406690643 1104 76-77 0.49313198399662284 1104 78-79 0.3623666314358225 1104 80-81 0.5758398403033027 1104 82-83 0.4981170723810777 1104 84-85 0.5402057786301349 1104 86-87 0.38515721242428214 1104 88-89 0.35551166552890123 1104 90-91 0.5938309905536769 1104 92-93 0.5252253324630693 1104 94-95 0.5464885970202893 1104 96-97 0.5759932546342874 1104 98-99 0.4005172747171315 1104 100-101 0.8617229185986659 1105 1 0.21596823854585168 1105 2 0.21987358880652508 1105 3 0.33135714278973083 1105 4 0.405994548266186 1105 5 0.5341560201061313 1105 6 0.2933330221382917 1105 7 0.4170549937134638 1105 8 0.3479631174044684 1105 9 0.3925875749841339 1105 10-11 0.4896931211953728 1105 12-13 0.49034157398796907 1105 14-15 0.7576962058704595 1105 16-17 0.7426239495985172 1105 18-19 0.46459872170118643 1105 20-21 0.4348253524615373 1105 22-23 0.46518585206014507 1105 24-25 0.40787286544146895 1105 26-27 0.4207499279171927 1105 28-29 0.5365673697983837 1105 30-31 0.3402733404107252 1105 32-33 0.34919167560734365 1105 34-35 0.33895823102566425 1105 36-37 0.2786641904963503 1105 38-39 0.17138847996653794 1105 40-41 0.25475547042528746 1105 42-43 0.09256002544928776 1105 44-45 0.10294412005057296 1105 46-47 0.3383205484691274 1105 48-49 0.378150516007544 1105 50-51 0.3684143933851445 1105 52-53 0.5645657584604749 1105 54-55 0.4235354551969124 1105 56-57 0.25079189401918 1105 58-59 0.31559712010675867 1105 60-61 0.2611523901152424 1105 62-63 0.4535610965910948 1105 64-65 0.5683087155016864 1105 66-67 0.44990498010299973 1105 68-69 0.5912130149006813 1105 70-71 0.5957242891648882 1105 72-73 0.49008263762752335 1105 74-75 0.454715166722675 1105 76-77 0.506743408522631 1105 78-79 0.49061864018826284 1105 80-81 0.5005504714418869 1105 82-83 0.5702664980680368 1105 84-85 0.5150045873374864 1105 86-87 0.6756322440554783 1105 88-89 0.546177485185229 1105 90-91 0.5712996200930576 1105 92-93 0.5444979781377874 1105 94-95 0.5702393884587416 1105 96-97 0.8577445538575432 1105 98-99 0.8183330415406616 1105 100-101 1.3547772231283801 1106 1 0.11002324318831924 1106 2 0.09663843944410644 1106 3 0.24629543148590471 1106 4 0.3256975813017604 1106 5 0.4132874418317911 1106 6 0.273216270100896 1106 7 0.34692961392638466 1106 8 0.5184930587483407 1106 9 0.478124036967543 1106 10-11 0.6176455218734489 1106 12-13 0.5945119103660659 1106 14-15 0.4835708720501657 1106 16-17 0.255859240811553 1106 18-19 0.49884774567016876 1106 20-21 0.254516067835759 1106 22-23 0.1967557308173653 1106 24-25 -0.08802765166688431 1106 26-27 0.008191587361146446 1106 28-29 0.19233335506136484 1106 30-31 0.15680231322011906 1106 32-33 0.25965816842619915 1106 34-35 0.202337590395949 1106 36-37 0.028541417887744558 1106 38-39 0.14408259490755881 1106 40-41 0.24667231938796164 1106 42-43 0.1753085840943669 1106 44-45 -0.08020693523723565 1106 46-47 0.2274854774657129 1106 48-49 0.3572965896452587 1106 50-51 0.18391279451319065 1106 52-53 0.2810003557187386 1106 54-55 0.34985985966222444 1106 56-57 0.5818842569075429 1106 58-59 0.4531342234704354 1106 60-61 0.5184791990383317 1106 62-63 0.49575519169097504 1106 64-65 0.11172936956231894 1106 66-67 0.1713761721276228 1106 68-69 0.17411644210142185 1106 70-71 0.025846160393616202 1106 72-73 0.12164899705293308 1106 74-75 0.14075835862016461 1106 76-77 -0.0030740907802382367 1106 78-79 0.07643215977325113 1106 80-81 0.2879141489568937 1106 82-83 0.22488923916425918 1106 84-85 0.6465124109378024 1106 86-87 0.7206299537964327 1106 88-89 0.6508265225133094 1106 90-91 0.7736996248333803 1106 92-93 0.7251977110815524 1106 94-95 0.897910098945399 1106 96-97 1.1038068973841924 1106 98-99 1.3453310748446725 1106 100-101 1.8799555284587868 1107 1 0.08740160686680198 1107 2 0.23193357019578897 1107 3 0.30048523939642635 1107 4 0.21627851171135148 1107 5 0.2681158889924191 1107 6 0.22189274790301994 1107 7 0.2421768073474979 1107 8 0.302032092690105 1107 9 0.3149340919193051 1107 10-11 0.426878721518392 1107 12-13 0.3131838192980254 1107 14-15 0.026871569232540082 1107 16-17 0.18962414021584806 1107 18-19 0.3903030633015234 1107 20-21 0.19721371712341096 1107 22-23 0.29177587717159525 1107 24-25 0.17140378896305464 1107 26-27 0.1412973484701041 1107 28-29 0.24492602775373484 1107 30-31 0.14625273471271072 1107 32-33 0.2600939297893845 1107 34-35 0.2649166997025887 1107 36-37 0.3897426511962365 1107 38-39 0.4712788661938916 1107 40-41 0.20737887224344576 1107 42-43 0.09159495915779559 1107 44-45 0.23380943463786963 1107 46-47 0.23853070319331948 1107 48-49 0.25345639339867887 1107 50-51 0.21004460140466819 1107 52-53 0.2629740861475156 1107 54-55 0.2046414902261091 1107 56-57 0.3139110700167578 1107 58-59 0.3366870248932301 1107 60-61 0.4295986510030687 1107 62-63 0.3219773555093184 1107 64-65 0.4508413151168611 1107 66-67 0.2104903197629966 1107 68-69 0.4931570473979434 1107 70-71 0.47876012205162866 1107 72-73 0.5089815001600151 1107 74-75 0.5611287880044458 1107 76-77 0.5575648599837422 1107 78-79 0.41487828917754754 1107 80-81 0.539505465668757 1107 82-83 0.4245391978260109 1107 84-85 0.46255478470217426 1107 86-87 0.6191142060539256 1107 88-89 0.5481144003626319 1107 90-91 0.540343506826197 1107 92-93 0.6315063325323962 1107 94-95 0.6398114543172078 1107 96-97 0.8260991704436442 1107 98-99 0.9631149185893122 1107 100-101 1.4368503151845715 1108 1 0.13145072384353895 1108 2 0.09015239639472128 1108 3 0.3334152157387571 1108 4 0.3152077124289008 1108 5 0.2486641527448441 1108 6 0.240808139980615 1108 7 0.20842453538544703 1108 8 0.22370361966714114 1108 9 -0.18366263467740396 1108 10-11 0.16389413659803864 1108 12-13 0.055521468695623355 1108 14-15 0.4420418677665907 1108 16-17 0.573194951536415 1108 18-19 0.3725751846279124 1108 20-21 0.2970940212542601 1108 22-23 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0.8042186980397403 2311 26-27 0.864891274080108 2311 28-29 1.0371601356591356 2311 30-31 1.0373365903067295 2311 32-33 0.8087275105360234 2311 34-35 0.8747100345511853 2311 36-37 0.9729795913011756 2311 38-39 0.8418636582137395 2311 40-41 1.0507536293300959 2311 42-43 1.1694413582976608 2311 44-45 1.2240429547738003 2311 46-47 1.0159488461602777 2311 48-49 1.0705144720680977 2311 50-51 1.152668673604765 2311 52-53 1.1313675307323905 2311 54-55 1.059270488494164 2311 56-57 0.8982784288326471 2311 58-59 1.0242681267052305 2311 60-61 1.0272512647675853 2311 62-63 1.0256520246235823 2311 64-65 1.2240348046771885 2311 66-67 1.2800102259901252 2311 68-69 1.203541964017198 2311 70-71 1.1812259549349697 2311 72-73 1.1731565406113909 2311 74-75 1.0228817887413264 2311 76-77 1.2467377168087914 2311 78-79 1.2302336218825793 2311 80-81 1.426348019213446 2311 82-83 1.235356327686869 2311 84-85 1.384380117759477 2311 86-87 1.4496686637642 2311 88-89 1.4341625241088565 2311 90-91 1.3183138297760877 2311 92-93 1.5084801015100417 2311 94-95 1.544264773212067 2311 96-97 1.6018664436517334 2311 98-99 1.792910174063941 2311 100-101 1.485129448740949 2312 1 0.2498523470442322 2312 2 0.29480930079623846 2312 3 0.37292799559491385 2312 4 0.4269839654706473 2312 5 0.3508610123173952 2312 6 0.2894479245935955 2312 7 0.3214234876431874 2312 8 0.49254623438392287 2312 9 0.5609634373088852 2312 10-11 0.41177790719046925 2312 12-13 0.2014133292979139 2312 14-15 0.49378001518233106 2312 16-17 0.5341608039367216 2312 18-19 0.4912736098153161 2312 20-21 0.3384026093415926 2312 22-23 0.5158111147755733 2312 24-25 0.30016417666961814 2312 26-27 0.38768796416573537 2312 28-29 0.31300272325881195 2312 30-31 0.367557110160746 2312 32-33 0.548795129851591 2312 34-35 0.38143158859062964 2312 36-37 0.693839560770563 2312 38-39 0.6592278464823806 2312 40-41 0.7019342158200388 2312 42-43 0.30304144374169084 2312 44-45 0.11615716062619441 2312 46-47 0.11270054006222807 2312 48-49 0.3439171505302099 2312 50-51 0.37041566678226445 2312 52-53 0.2585471025744184 2312 54-55 0.1339581488645365 2312 56-57 0.41764400686936654 2312 58-59 0.5249674066829328 2312 60-61 0.3187875185676319 2312 62-63 0.3966631167181873 2312 64-65 0.38908116067143084 2312 66-67 0.2718349185348643 2312 68-69 0.30246867100928654 2312 70-71 0.22789487492756777 2312 72-73 0.16650222816235072 2312 74-75 0.07376234423176342 2312 76-77 0.10804029549936445 2312 78-79 0.10291403123244791 2312 80-81 -0.12363201996577189 2312 82-83 0.0561231358050982 2312 84-85 -0.11741016923349079 2312 86-87 -0.24658853580753615 2312 88-89 0.07153106924180008 2312 90-91 0.27243455073618605 2312 92-93 0.24348945997642346 2312 94-95 0.08455274305657312 2312 96-97 0.21550114956013644 2312 98-99 0.3147632025079474 2312 100-101 0.14898141173951274 2313 1 -0.06439102313733613 2313 2 -0.20073979518100415 2313 3 0.02363823100358431 2313 4 -0.05196433244637433 2313 5 -0.2093825106029712 2313 6 -0.022332532477257416 2313 7 0.09632468473351707 2313 8 -0.026083125831441123 2313 9 -0.07186203952254289 2313 10-11 0.05107807711238621 2313 12-13 0.1549986339163567 2313 14-15 0.05388989165482627 2313 16-17 0.24819917001342873 2313 18-19 -0.009245463442027813 2313 20-21 0.051625970740680316 2313 22-23 0.18046950490865044 2313 24-25 0.016707220372005338 2313 26-27 -0.07745895146517512 2313 28-29 0.052432721158652384 2313 30-31 -0.11864628064056149 2313 32-33 0.06650089765043532 2313 34-35 0.03292103052124418 2313 36-37 0.017170889407175594 2313 38-39 0.10748877207949192 2313 40-41 0.21245529377410577 2313 42-43 0.4020973270492547 2313 44-45 0.5798005537185773 2313 46-47 0.44449216615765863 2313 48-49 0.42635314342785335 2313 50-51 0.383737353782287 2313 52-53 0.4360021400908849 2313 54-55 0.2779073059373047 2313 56-57 0.5964868195543218 2313 58-59 0.48853204383282645 2313 60-61 0.4035670973904004 2313 62-63 0.3209965550594376 2313 64-65 0.22080238372734584 2313 66-67 0.405492105849909 2313 68-69 0.22905381334275177 2313 70-71 -0.04574264726728572 2313 72-73 0.275475393864852 2313 74-75 0.19664547995927606 2313 76-77 0.33773558159822414 2313 78-79 0.3344672416110477 2313 80-81 0.32422319320031434 2313 82-83 0.21566119640421633 2313 84-85 0.04982568810847354 2313 86-87 0.20743111976647555 2313 88-89 0.30019536845710704 2313 90-91 0.25902575489067914 2313 92-93 0.36832001537287695 2313 94-95 0.2643704588478535 2313 96-97 0.1944566071082967 2313 98-99 0.3449577535513484 2313 100-101 0.2616751990889199 2314 1 -0.453154421273954 2314 2 -0.23588102963457658 2314 3 -0.38482895333850564 2314 4 -0.11029406791465135 2314 5 -0.1687785177027621 2314 6 -0.011623408917181166 2314 7 -0.2957245027746964 2314 8 -0.22589838328214995 2314 9 -0.4770465630713545 2314 10-11 -0.34615490394303094 2314 12-13 -0.7162023595115485 2314 14-15 -1.0315037408540206 2314 16-17 -1.2320327775682784 2314 18-19 -1.453032732631165 2314 20-21 -1.3019782036170753 2314 22-23 -1.2459074866921682 2314 24-25 -1.0761595428634436 2314 26-27 -0.8390839663259158 2314 28-29 -0.8183283159441999 2314 30-31 -1.0191852564691644 2314 32-33 -0.8839803559083279 2314 34-35 -1.159625105580254 2314 36-37 -0.8834233579715942 2314 38-39 -0.9526276423194773 2314 40-41 -0.9991855986215725 2314 42-43 -0.9113773554521885 2314 44-45 -0.8470306031671058 2314 46-47 -1.0726962430854101 2314 48-49 -1.363239015103467 2314 50-51 -0.8644197832348439 2314 52-53 -0.8322429464768746 2314 54-55 -1.025653433324898 2314 56-57 -0.8687927397749959 2314 58-59 -1.0521101437942733 2314 60-61 -1.1783963097148131 2314 62-63 -0.9839549676196242 2314 64-65 -0.8168202043994839 2314 66-67 -0.8391887895909207 2314 68-69 -0.6447655394169693 2314 70-71 -0.9278097146005528 2314 72-73 -0.756192075803579 2314 74-75 -0.6758146358815509 2314 76-77 -0.680782473427211 2314 78-79 -0.5262187304695196 2314 80-81 -0.4596215062580278 2314 82-83 -0.5248115004928593 2314 84-85 -0.7482041157713795 2314 86-87 -0.5360529641127982 2314 88-89 -0.5022122289824651 2314 90-91 -0.5475395927673716 2314 92-93 -0.5748995472101619 2314 94-95 -0.7591247731987991 2314 96-97 -0.7735953940346754 2314 98-99 -0.9602520071958409 2314 100-101 -1.5202455550653085 2315 1 -0.9724175052239445 2315 2 -1.3903327193211332 2315 3 -1.2070715596412462 2315 4 -0.9422089403119855 2315 5 -0.926474992720145 2315 6 -0.5893366645733593 2315 7 -0.6324852257486455 2315 8 -0.35266687772232075 2315 9 -0.8449839330834195 2315 10-11 -1.080455404216785 2315 12-13 -0.9929569553275357 2315 14-15 -0.968124352398597 2315 16-17 -1.1835777240822338 2315 18-19 -1.2366302591619345 2315 20-21 -1.423622107823725 2315 22-23 -1.1731065699711323 2315 24-25 -1.279956079280268 2315 26-27 -1.4075968953708156 2315 28-29 -1.3945992856692442 2315 30-31 -1.3050084808016535 2315 32-33 -1.4571862123052384 2315 34-35 -1.1550747446123992 2315 36-37 -1.6352232106231668 2315 38-39 -1.6104571963436953 2315 40-41 -1.6270277521897398 2315 42-43 -1.460689469452106 2315 44-45 -1.4497208500153675 2315 46-47 -1.9985752767231517 2315 48-49 -2.4040164532507546 2315 50-51 -2.4016939241988595 2315 52-53 -2.047799355583841 2315 54-55 -2.252406737732386 2315 56-57 -2.455855792986906 2315 58-59 -2.634513641695669 2315 60-61 -2.8437252391320484 2315 62-63 -2.991666338586562 2315 64-65 -2.2021340511883167 2315 66-67 -2.1817152040092758 2315 68-69 -2.1789927234892232 2315 70-71 -1.9459858417508435 2315 72-73 -1.8150934733121566 2315 74-75 -1.9381008534329958 2315 76-77 -1.8612598802994 2315 78-79 -1.9552427913278443 2315 80-81 -2.1095811401526667 2315 82-83 -2.2155526641260828 2315 84-85 -2.1668169947198663 2315 86-87 -2.228069681331924 2315 88-89 -2.6318714326718506 2315 90-91 -2.3081894295256618 2315 92-93 -2.3123095290051374 2315 94-95 -2.4730339332232347 2315 96-97 -2.1068829953111496 2315 98-99 -2.2290398911757023 2315 100-101 -2.727446806887663 2316 1 -5.339189513232128 2316 2 -5.465039430003429 2316 3 -6.165206350934316 2316 4 -7.961545040474604 2316 5 -8.66786248259972 2316 6 -9.403526833436555 2316 7 -12.049848046151425 2316 8 -11.867692083088638 2316 9 -11.688184341844167 2316 10-11 -11.183015175616987 2316 12-13 -10.21236906016701 2316 14-15 -10.309823661541895 2316 16-17 -9.154297944516092 2316 18-19 -9.437642040611806 2316 20-21 -10.993416932894071 2316 22-23 -10.316329278463474 2316 24-25 -10.251716035573956 2316 26-27 -8.268556328998233 2316 28-29 -6.594161004598664 2316 30-31 -8.72961508917777 2316 32-33 -6.433648106152713 2316 34-35 -4.994919029269482 2316 36-37 -5.356326064292851 2316 38-39 -4.260657052975851 2316 40-41 -3.9801392172096044 2316 42-43 -4.108266715258363 2316 44-45 -4.489149182331822 2316 46-47 -4.474527814542803 2316 48-49 -5.2119756015343945 2316 50-51 -5.173078032870762 2316 52-53 -5.137336112733095 2316 54-55 -4.337459479932406 2316 56-57 -4.793042233822149 2316 58-59 -5.105223277731572 2316 60-61 -5.291025956667237 2316 62-63 -4.965722239286926 2316 64-65 -5.5269471858172174 2316 66-67 -4.894720993239986 2316 68-69 -5.272446867817493 2316 70-71 -5.056460277964913 2316 72-73 -4.165398477963496 2316 74-75 -4.6267010508537645 2316 76-77 -4.923805117726296 2316 78-79 -5.359212868509854 2316 80-81 -5.793430682790682 2316 82-83 -5.2366540388798875 2316 84-85 -5.129421156795125 2316 86-87 -5.794702236272201 2316 88-89 -7.23763740670325 2316 90-91 -6.39971532853253 2316 92-93 -5.706154687092546 2316 94-95 -5.34703117026039 2316 96-97 -6.752120799184869 2316 98-99 -8.282402655901645 2316 100-101 -8.549227149021807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6247.0 3 2411.0 4 2490.0 5 2736.0 6 3003.0 7 3365.0 8 4025.0 9 5060.0 10 6096.0 11 7295.0 12 8402.0 13 9633.0 14 10430.0 15 11889.0 16 13402.0 17 15056.0 18 13921.0 19 13461.0 20 14591.0 21 16235.0 22 17971.0 23 20871.0 24 23879.0 25 27081.0 26 32213.0 27 37807.0 28 44436.0 29 53800.0 30 64931.0 31 79520.0 32 98577.0 33 124884.0 34 162562.0 35 218021.0 36 294924.0 37 365283.0 38 257673.0 39 29772.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.030743063375475 17.867161696734403 13.444542340619007 23.65755289927112 2 15.912683013209353 19.416401675847155 39.48936206285934 25.181553248084153 3 20.564048666419925 23.002219729695877 28.761368227446482 27.672363376437715 4 12.904972738891415 15.388516711262055 36.54712088976039 35.159389660086134 5 14.409564180099773 37.05926704194124 33.61172032138674 14.919448456572242 6 33.67300851550626 34.313237441853566 17.324376219503293 14.689377823136876 7 30.582067969888538 31.367223886757372 19.73726677726572 18.31344136608837 8 27.45871425226832 33.24590275137823 19.29544894614587 19.999934050207578 9 26.45689309529093 16.826550462925663 19.96179938647214 36.754757055311266 10-11 25.84470727681129 25.326213744082978 27.027759143124193 21.80131983598155 12-13 27.000300030285633 22.544379283497534 27.275131539965493 23.18018914625134 14-15 23.296280455694344 24.39180462382984 25.52823784433552 26.783677076140293 16-17 23.240816026576546 27.916137092314113 25.872207780950774 22.970839100158567 18-19 23.032144887762104 26.84627367740738 27.453709788271187 22.66787164655933 20-21 23.860627939020016 25.702751294746328 27.14724460840222 23.289376157831438 22-23 23.364342297146425 25.710074036333978 27.329097932039893 23.596485734479707 24-25 23.712789611006084 25.903259993506055 27.268754445750716 23.115195949737146 26-27 23.31253486327943 25.96345092133243 27.63505172068312 23.08896249470502 28-29 22.811400944938686 26.710138209466194 27.4451506856797 23.03331015991542 30-31 22.981351106649786 25.916377776511784 27.448723665012032 23.653547451826398 32-33 22.876254621546437 26.123979869551377 27.45888401072067 23.540881498181516 34-35 23.792810128060456 25.964726575752444 27.038642271463303 23.20382102472379 36-37 23.606533526439396 25.569006853507453 27.423446566560383 23.401013053492765 38-39 23.247859183187717 25.9731879071307 27.484007881228777 23.294945028452798 40-41 23.38579190358716 26.108538366229705 27.347999509385357 23.157670220797776 42-43 23.166596159962406 26.157328118426783 26.850600884264715 23.825474837346093 44-45 23.233374969573216 25.975081346038586 26.964605115654738 23.82693856873346 46-47 23.62060994017739 26.229221458549322 26.72384326155185 23.426325339721444 48-49 23.66793490773511 26.285524207616135 26.67190536074718 23.37463552390158 50-51 23.005330669097372 26.376289116056228 27.112092831422412 23.50628738342398 52-53 23.637858276524952 26.53479149491538 26.4560703556058 23.371279872953874 54-55 23.051739622237314 25.968447976119997 26.88610032474832 24.093712076894374 56-57 23.45441152270931 26.254316696058687 26.591930647535598 23.699341133696397 58-59 24.02715310286704 25.84248422405665 26.527738704942482 23.60262396813383 60-61 23.470496877959686 25.97917038926686 27.41083828130168 23.139494451471773 62-63 23.189676359832045 26.198737041539584 27.165707268979457 23.445879329648918 64-65 22.995594049942888 26.584032739062103 26.795808368009354 23.624564842985652 66-67 23.090866631386454 26.435510662219603 26.37903564494046 24.09458706145348 68-69 22.932728469072707 26.14296627536469 27.046966830948367 23.87733842461424 70-71 23.343707257587994 26.079198084669112 26.77720153584301 23.79989312189989 72-73 23.24731631421021 25.811274588288036 27.273645536859593 23.667763560642165 74-75 22.832384096137048 26.73121793528549 26.76903045956579 23.667367509011672 76-77 23.08775052742616 26.17260492013261 26.67867503013864 24.060969522302592 78-79 23.237341435367224 26.151309438486482 26.927497972764886 23.68385115338141 80-81 23.316316152290113 26.542235004727516 26.66581439500955 23.47563444797282 82-83 23.24966953131713 26.296220003272317 26.941549124184423 23.512561341226128 84-85 23.064025854421978 26.434724552849204 26.644433897768817 23.856815694960005 86-87 23.219101589131082 26.315863814592582 26.720114806151624 23.74491979012471 88-89 23.077136759933566 26.327090560036915 26.325513289679826 24.270259390349693 90-91 23.06906709941066 26.05840960932738 26.77530468217165 24.097218609090305 92-93 23.365231447714198 25.97006885491216 26.993286846183224 23.671412851190425 94-95 23.004568493499008 25.859217696108068 27.077765158150303 24.05844865224262 96-97 22.66051326386034 25.896606069681116 27.369340251438175 24.073540415020368 98-99 22.763699861477356 26.243978338382828 26.596042015004144 24.39627978513567 100-101 22.525749711275957 25.800310091391022 27.05115330687628 24.622786890456744 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 41.0 1 33.5 2 42.5 3 64.0 4 64.5 5 65.5 6 118.0 7 231.5 8 366.5 9 459.5 10 463.5 11 463.5 12 492.0 13 542.5 14 631.5 15 685.0 16 761.5 17 861.0 18 935.0 19 1038.0 20 1189.5 21 1384.0 22 1667.5 23 2089.5 24 2689.5 25 3503.5 26 4601.0 27 6206.0 28 8228.5 29 10764.5 30 14265.0 31 18619.0 32 23699.5 33 28042.0 34 32736.5 35 37538.0 36 42332.0 37 47435.0 38 51414.5 39 54472.5 40 57708.0 41 63089.0 42 68649.5 43 73374.0 44 76801.0 45 80486.5 46 83082.0 47 90044.0 48 107468.0 49 109066.5 50 94574.0 51 85886.0 52 81044.0 53 78857.5 54 77394.0 55 72560.5 56 65523.5 57 58939.0 58 53710.0 59 47638.5 60 40320.0 61 33634.5 62 27807.0 63 21997.0 64 16883.5 65 13369.0 66 10095.5 67 8077.5 68 6102.0 69 3968.0 70 3039.5 71 2492.5 72 2073.0 73 1814.0 74 1416.0 75 1169.5 76 902.5 77 564.5 78 353.0 79 236.5 80 165.5 81 113.0 82 76.0 83 51.5 84 31.5 85 19.0 86 14.0 87 12.0 88 9.0 89 9.5 90 7.5 91 2.5 92 4.5 93 5.0 94 2.0 95 0.0 96 1.0 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0718000145954128 2 0.028767087814948997 3 0.012853379661998489 4 0.019633184318876812 5 0.025047611649022694 6 0.030697448863087964 7 0.07410703145782278 8 0.05310846981221353 9 0.06949299773300281 10-11 0.05965286458517248 12-13 0.03936053259132138 14-15 0.04486912387503502 16-17 0.05560381458175903 18-19 0.051696010508697224 20-21 0.017561577340386214 22-23 0.012617969778079103 24-25 0.020645446819730174 26-27 0.022669971821436895 28-29 0.005343804364970068 30-31 0.0010358034892452995 32-33 0.00974596919426259 34-35 0.010899477625467583 36-37 0.003719476165926303 38-39 0.00821580494878658 40-41 0.0041667549453731365 42-43 0.00204806599009866 44-45 1.88327907135509E-4 46-47 4.237377910548953E-4 48-49 7.53311628542036E-4 50-51 8.945575588936676E-4 52-53 9.41639535677545E-5 54-55 1.1770494195969312E-4 56-57 0.003743017154318241 58-59 0.0034134433168311004 60-61 0.004872984597131295 62-63 0.002495344769545494 64-65 4.237377910548953E-4 66-67 3.295738374871407E-4 68-69 0.002942623548992328 70-71 0.0018832790713550899 72-73 0.018032397108224987 74-75 0.015066232570840719 76-77 0.020480659900986603 78-79 0.017561577340386214 80-81 0.010310952915669117 82-83 0.003648853200750487 84-85 0.0029661645373842667 86-87 0.0012712133731646856 88-89 0.0015301642454760105 90-91 0.0067562636684863845 92-93 0.01007554303174973 94-95 0.013135871522701752 96-97 0.011864658149537067 98-99 0.02238747996073363 100-101 0.018244266003752434 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2123955.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.2060762016657 #Duplication Level Percentage of deduplicated Percentage of total 1 78.05627805254517 43.091808341872145 2 12.605407875556251 13.917902154620707 3 4.063076696644959 6.729195651845813 4 1.7575517613259275 3.8811014585652366 5 0.9258389997792869 2.5555969186144627 6 0.547579244912538 1.8137820912655276 7 0.3662349556685243 1.4152876409245145 8 0.2673241802787968 1.180633525361526 9 0.18867614786236214 0.9374462816693678 >10 1.0588471581840677 10.868422678678629 >50 0.09678892219220786 3.6928845614083485 >100 0.06090835254492803 6.081279426865987 >500 0.003429782815653822 1.3095563290219114 >1k 0.0018863805486095998 1.8725259086733677 >5k 1.7148914078269088E-4 0.6525770306125447 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8234 0.38767299683844525 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5552 0.26139913510408647 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4120 0.19397774434957427 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4046 0.19049367806756734 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2634 0.12401392684873266 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2544 0.11977654893818371 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2326 0.10951267799929848 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.708197678387725E-5 2 0.0 0.0 0.0 0.0 4.708197678387725E-5 3 0.0 0.0 0.0 0.0 4.708197678387725E-5 4 0.0 0.0 0.0 4.708197678387725E-5 4.708197678387725E-5 5 0.0 0.0 0.0 4.708197678387725E-5 4.708197678387725E-5 6 0.0 0.0 0.0 4.708197678387725E-5 4.708197678387725E-5 7 0.0 0.0 0.0 4.708197678387725E-5 4.708197678387725E-5 8 0.0 0.0 0.0 4.708197678387725E-5 4.708197678387725E-5 9 0.0 0.0 0.0 9.41639535677545E-5 4.708197678387725E-5 10-11 0.0 4.708197678387725E-5 0.0 1.4124593035163175E-4 4.708197678387725E-5 12-13 0.0 4.708197678387725E-5 0.0 1.88327907135509E-4 1.88327907135509E-4 14-15 0.0 4.708197678387725E-5 0.0 1.88327907135509E-4 3.295738374871407E-4 16-17 0.0 4.708197678387725E-5 0.0 2.3540988391938623E-4 4.2373779105489524E-4 18-19 0.0 4.708197678387725E-5 0.0 3.531148258790794E-4 4.2373779105489524E-4 20-21 0.0 4.708197678387725E-5 0.0 3.76655814271018E-4 4.2373779105489524E-4 22-23 0.0 4.708197678387725E-5 0.0 4.001968026629566E-4 4.4727877944683385E-4 24-25 0.0 4.708197678387725E-5 0.0 4.4727877944683385E-4 4.7081976783877247E-4 26-27 0.0 4.708197678387725E-5 0.0 6.356066865823428E-4 5.179017446226498E-4 28-29 0.0 4.708197678387725E-5 0.0 0.0013889183151243787 5.414427330145883E-4 30-31 0.0 4.708197678387725E-5 0.0 0.0031074104677358983 5.64983721406527E-4 32-33 0.0 4.708197678387725E-5 0.0 0.0057675421560249625 5.64983721406527E-4 34-35 0.0 4.708197678387725E-5 0.0 0.009651805240694835 6.120656981904042E-4 36-37 0.0 4.708197678387725E-5 0.0 0.01353606832536471 6.120656981904042E-4 38-39 0.0 4.708197678387725E-5 0.0 0.017538036351994274 6.120656981904042E-4 40-41 0.0 4.708197678387725E-5 0.0 0.020810233738473745 6.120656981904042E-4 42-43 0.0 4.708197678387725E-5 0.0 0.02533010350972596 6.120656981904042E-4 44-45 0.0 4.708197678387725E-5 0.0 0.029473317466707158 6.120656981904042E-4 46-47 0.0 4.708197678387725E-5 0.0 0.037171220670871086 6.120656981904042E-4 48-49 0.0 4.708197678387725E-5 0.0 0.04562243550357705 6.591476749742814E-4 50-51 0.0 4.708197678387725E-5 0.0 0.05310846981221354 6.591476749742814E-4 52-53 0.0 4.708197678387725E-5 0.0 0.06527916081084581 7.297706401500973E-4 54-55 0.0 4.708197678387725E-5 0.0 0.09065634629735564 7.768526169339746E-4 56-57 0.0 4.708197678387725E-5 0.0 0.13931556930349276 8.239345937178519E-4 58-59 0.0 4.708197678387725E-5 0.0 0.18879872690334776 8.474755821097905E-4 60-61 0.0 4.708197678387725E-5 0.0 0.2340445065926538 8.945575588936677E-4 62-63 0.0 4.708197678387725E-5 0.0 0.29054287873330653 8.945575588936677E-4 64-65 0.0 4.708197678387725E-5 0.0 0.35848217123244136 8.945575588936677E-4 66-67 0.0 4.708197678387725E-5 0.0 0.4725853419681678 8.945575588936677E-4 68-69 0.0 4.708197678387725E-5 0.0 0.6166091089500484 9.416395356775449E-4 70-71 0.0 4.708197678387725E-5 0.0 0.7340551000374302 9.416395356775449E-4 72-73 0.0 4.708197678387725E-5 0.0 0.8540435178711414 9.416395356775449E-4 74-75 0.0 4.708197678387725E-5 0.0 0.9873090531579058 9.416395356775449E-4 76-77 0.0 4.708197678387725E-5 0.0 1.1378301329359615 9.416395356775449E-4 78-79 0.0 4.708197678387725E-5 0.0 1.254240320534098 9.416395356775449E-4 80-81 0.0 4.708197678387725E-5 0.0 1.3559844723640566 9.416395356775449E-4 82-83 0.0 4.708197678387725E-5 0.0 1.490474139047202 9.887215124614222E-4 84-85 0.0 4.708197678387725E-5 0.0 1.7166324145285565 9.887215124614222E-4 86-87 0.0 4.708197678387725E-5 0.0 2.031728544154655 9.887215124614222E-4 88-89 0.0 4.708197678387725E-5 0.0 2.4050415380740175 9.887215124614222E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8660 0.0 28.157394 1 TACATGG 8925 0.0 26.520603 2 ACATGGG 9130 0.0 24.783342 3 ACCGTCG 185 2.5200097E-7 23.130585 8 CATGGGG 4355 0.0 21.93923 4 ATACCGT 230 8.961797E-8 20.6669 6 GTGTAGC 1300 0.0 20.11001 1 TACGAAC 120 0.004743901 19.806715 3 GAGTACT 3580 0.0 19.054056 12-13 CATGGGA 4655 0.0 18.58511 4 TAGCCCT 1365 0.0 18.45681 4 TACCGTC 285 4.242429E-8 18.346407 7 GTAGCCC 1100 0.0 17.718008 3 TAAGGTG 795 0.0 17.340218 5 GTACTTT 3940 0.0 17.007847 14-15 AGTACAT 6590 0.0 16.732655 2 ATGGGGA 2785 0.0 16.727251 5 GAGTACA 7690 0.0 16.56538 1 AGTACTT 3960 0.0 16.445381 12-13 CTCGCGC 435 0.0 16.374592 60-61 >>END_MODULE