FastQCFastQC Report
Thu 26 May 2016
SRR936548_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936548_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123955
Sequences flagged as poor quality0
Sequence length101
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT108500.5108394481050682No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42300.19915676179580077No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38030.1790527577090852No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32880.1548055396653884No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30390.14308212744620297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30220.14228173384087706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27390.12895753441103977No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27070.1274509111539557No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC25660.12081235242742902No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24000.11299674428130539No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG22820.10744107102080788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG87750.027.615381
TACATGG93600.025.6313742
ACATGGG90650.025.3095573
CATGGGG43450.021.8622234
TACACCG2205.6503268E-821.5889455
GAGTACT49400.020.0933812-13
AATACCG1200.00476731319.7898675
TAAGGTG8200.019.693335
GTACTTT51250.019.2744814-15
CATGGGA45950.018.605494
TACACTG16200.017.5909925
ATACCGA1350.00941297617.5893366
CTTACAC8400.017.5302313
CTCGCGC4750.017.50067360-61
AGTACTT51950.017.37007512-13
ATTCGCG4650.017.36646580-81
CGCGGAA4800.017.32184282-83
ATGGGGA27300.017.223715
ATGGGTA8850.017.173585
TGAACCG3903.0195224E-1017.0497325
Produced by FastQC (version 0.11.5)