FastQCFastQC Report
Thu 26 May 2016
SRR936547_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936547_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215117
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.2691558547209193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.2449829627598005No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4110.1910588191542277No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2930.13620494893476573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.13574008562782114No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2720.12644281948892927No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2480.11528610012225904No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAC550.002574859434.545791
CATATAG600.003939194631.6743412
TCTAAAC1102.7147053E-630.23463
TACATGG6500.028.5069082
GTGCTAG1205.3546228E-627.7086051
GTCCATA700.008376270527.1431241
GTACATG6850.027.044061
GTAAGGT908.9111883E-426.3952854
GTCTAAG1109.4409144E-525.9093441
ACATGGG6800.025.8518543
TAGGCAG950.001221585225.006065
GTATCAA9650.024.1194081
CTAAACT1201.7017213E-423.7557564
GTGTAAG1000.001649197823.7502331
GGAGTGT1452.3185938E-522.9365926
CTACACA1503.0127943E-522.172044
GTACAGC1302.9259533E-421.928396
GTAGGGC1100.002865810621.5961423
TAAGGTG1100.002865810621.5961425
GGTATCA7050.021.5604951
Produced by FastQC (version 0.11.5)