FastQCFastQC Report
Thu 26 May 2016
SRR936547_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936547_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215117
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8150.37886359515984325No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.300301696286207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5640.2621829051167504No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC4890.22731815709590594No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA4490.20872362481812223No Hit
CCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAA4060.18873450261950472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.1501508481431035No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG2570.11946986988476038No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA2520.11714555335003742No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT2230.10366451744864423No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT2220.10319965414169964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT1600.053.4347574
GGTAAGG1750.046.1404843
AAGGTAA1650.046.068951
CTATACA1006.311893E-1042.7478034
TAAGGTG2000.042.7478035
AGGTAAG1900.039.9979442
CCTATAC500.001613303837.9980473
TTATACT905.671591E-736.9425474
AAGGTGT2600.032.8829276
GGTGTGC2900.032.756948
TACTATG804.4829867E-429.6859762
GTATGAT650.00582849229.2360651
GCTATAC650.005835119629.2292673
GACCCTA650.005835119629.2292677
AGGTGTG3100.029.1114067
ATTGGTC1850.028.2417920-21
GTGTGCA3250.027.7678039
GAATAGA700.00836928127.1477721
TATACTA908.926502E-426.3875335
CTACAGG950.001222023525.0045261
Produced by FastQC (version 0.11.5)