FastQCFastQC Report
Thu 26 May 2016
SRR936546_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936546_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303460
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8210.2705463652540697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.2217755223093653No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4940.1627891649640809No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4100.13510841626573517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4080.13444935082053647No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3620.11929084558096618No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT3530.11632505107757202No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGA250.005008355257.026912
GCCGGTC459.5837546E-442.2491268
GTGTAGG902.399396E-531.681621
GTACATG9250.027.2290671
TATGGGT700.008362723527.1556724
TCCTATA1109.423796E-525.9213242
GTATCAA15950.025.6233771
TAAGGCG950.00122110825.0118035
TACATGG9850.024.1230582
TATGCCC1000.001646319523.7612134
ACATGGG10050.023.6433
GAGTACT9300.023.50959412-13
GGTATCA11800.023.358481
GAAGTTA1252.2409006E-422.8145286
CTAGGAG1703.1235031E-622.3634933
TCAACGC18250.022.1337344
TATCAAC18300.022.073262
CCAGTAC1302.9252472E-421.933433
ATCAACG18550.021.7757743
ATCTAGG1100.002864678421.6011031
Produced by FastQC (version 0.11.5)