FastQCFastQC Report
Thu 26 May 2016
SRR936546_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936546_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303460
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10660.3512818822909115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8630.2843867396032426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7900.2603308508534898No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC5320.1753114084228564No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA5090.16773215580307124No Hit
CCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAA4370.14400579977591776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.1400514071047255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAG1800.036.930092
TAAGGTG2050.034.748325
GTAAGGT2200.032.3791164
GGTATAT600.003793854531.9226231
CCTAGAC902.4108404E-531.6595823
GGTATAC600.003953924431.6543642
GATAGGG804.4918162E-429.6808577
TATAGGT650.0058437729.2242283
GTATAGG1004.6772613E-528.730361
GGTAAGG2350.028.291543
AAGGTAA2400.027.9322951
TACTACC700.00839116327.1367844
GGTGTGC3200.026.7127728
CCATAAC908.943992E-426.3829863
AAGTCGG651.0041131E-525.56698866-67
AAGGTGT2850.024.9944066
GTATAAT1201.6160608E-423.9419671
GTACATG10400.023.4815431
GTATGGT1050.002195377822.6139853
AGGTGTG3600.022.4255377
Produced by FastQC (version 0.11.5)