##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936546_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 303460 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.66029789758123 31.0 31.0 34.0 30.0 34.0 2 31.89289527450076 31.0 31.0 34.0 30.0 34.0 3 32.12485994859289 33.0 31.0 34.0 30.0 34.0 4 35.892773347393394 37.0 35.0 37.0 35.0 37.0 5 35.72849139919594 37.0 35.0 37.0 33.0 37.0 6 35.809582811573186 37.0 35.0 37.0 35.0 37.0 7 35.747930534502075 37.0 35.0 37.0 33.0 37.0 8 35.71567257628683 37.0 35.0 37.0 33.0 37.0 9 37.38888815659395 39.0 37.0 39.0 34.0 39.0 10-11 37.2643890463323 39.0 37.0 39.0 33.5 39.0 12-13 37.155038555328545 39.0 37.0 39.0 33.0 39.0 14-15 38.32971561326039 40.0 38.0 41.0 33.0 41.0 16-17 38.24446879325117 40.0 38.0 41.0 33.0 41.0 18-19 38.275514071047255 40.0 38.0 41.0 33.5 41.0 20-21 38.31473505569103 40.0 38.0 41.0 34.0 41.0 22-23 38.23257595729256 40.0 38.0 41.0 33.5 41.0 24-25 38.13123146378435 40.0 37.5 41.0 33.0 41.0 26-27 37.90808508534897 40.0 37.0 41.0 33.0 41.0 28-29 37.83837738087392 40.0 37.0 41.0 33.0 41.0 30-31 37.861228827522574 40.0 37.0 41.0 33.0 41.0 32-33 37.70123739537336 40.0 37.0 41.0 33.0 41.0 34-35 37.54607196994662 40.0 37.0 41.0 32.5 41.0 36-37 37.324579845778686 40.0 36.0 41.0 31.5 41.0 38-39 37.13016707309036 39.0 36.0 41.0 31.0 41.0 40-41 36.90896823304554 39.0 36.0 41.0 31.0 41.0 42-43 36.699772622421406 39.0 35.5 41.0 30.5 41.0 44-45 36.42514334673433 39.0 35.0 40.5 30.0 41.0 46-47 36.05447175904567 39.0 35.0 40.0 29.5 41.0 48-49 35.48014565346339 38.5 35.0 40.0 27.0 41.0 50-51 35.40915606669742 38.0 35.0 40.0 27.5 41.0 52-53 35.16563303236011 38.0 34.0 40.0 27.0 41.0 54-55 34.91628385948725 38.0 34.0 40.0 26.0 41.0 56-57 34.62313978778093 38.0 34.0 40.0 26.0 41.0 58-59 34.221933698016215 37.5 33.5 40.0 25.0 41.0 60-61 33.832719304026895 37.0 33.0 40.0 23.5 41.0 62-63 33.544645093257756 36.5 33.0 39.0 23.0 41.0 64-65 33.11002438542147 36.0 32.0 39.0 22.5 40.0 66-67 32.7060469254597 35.5 32.0 39.0 22.0 40.0 68-69 32.224108613985365 35.0 31.0 38.0 20.5 40.0 70-71 31.735004613458116 35.0 31.0 37.5 19.5 39.5 72-73 31.294676398866407 35.0 30.0 37.0 18.0 39.0 74-75 30.923220523297964 34.0 30.0 36.5 17.0 39.0 76-77 29.51644038753048 33.0 28.5 35.0 13.5 37.5 78-79 30.3198658801819 34.0 30.0 36.0 14.0 38.0 80-81 30.151548803796217 34.0 30.0 35.0 10.5 37.0 82-83 29.815287023001382 34.0 29.5 35.0 7.5 37.0 84-85 29.381831213339485 34.0 29.0 35.0 4.5 36.0 86-87 28.996167534436168 34.0 29.0 35.0 2.0 36.0 88-89 28.582475449812165 34.0 29.0 35.0 2.0 35.5 90-91 28.139664206155672 33.0 27.5 35.0 2.0 35.0 92-93 27.68939728465037 33.0 27.0 35.0 2.0 35.0 94-95 27.163517432281026 33.0 26.0 34.5 2.0 35.0 96-97 26.481681275950702 32.5 25.0 34.0 2.0 35.0 98-99 25.653684175838663 32.0 23.5 34.0 2.0 35.0 100-101 22.9555526263758 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4870080030733597 1101 2 0.4389540530831937 1101 3 0.2916406243598928 1101 4 0.20461135489340876 1101 5 0.14465858952058852 1101 6 0.1854779619057112 1101 7 0.17510135128935644 1101 8 0.23482052500017403 1101 9 0.3765582749626546 1101 10-11 0.2958965638540292 1101 12-13 0.29119537005954754 1101 14-15 0.5096365589638552 1101 16-17 0.20786130017499005 1101 18-19 0.4914729163341107 1101 20-21 0.18912472380309708 1101 22-23 0.07650222427879783 1101 24-25 0.15849039730180436 1101 26-27 0.16727905553616296 1101 28-29 0.32623507396319695 1101 30-31 0.14166899609858064 1101 32-33 0.3156712707544358 1101 34-35 0.48084990085600765 1101 36-37 0.45715660875590913 1101 38-39 0.38686738686545397 1101 40-41 0.42380856975054115 1101 42-43 0.352960573667886 1101 44-45 0.6627775501316577 1101 46-47 0.7906323327623639 1101 48-49 0.6474601889182239 1101 50-51 0.5335130036869415 1101 52-53 0.49770148536347136 1101 54-55 0.5639526517513502 1101 56-57 0.6412288538972746 1101 58-59 0.7592526546108971 1101 60-61 0.7066642882197058 1101 62-63 0.5082926571602471 1101 64-65 0.6943901019828189 1101 66-67 0.524190967754798 1101 68-69 0.48759592421229314 1101 70-71 0.5483766453366492 1101 72-73 0.6563364990978755 1101 74-75 0.3794783167851783 1101 76-77 0.7646863606041343 1101 78-79 0.9305772879936534 1101 80-81 0.7052793766044019 1101 82-83 0.8841702702459457 1101 84-85 1.0288066311206698 1101 86-87 0.9632129028244414 1101 88-89 0.6594292270867683 1101 90-91 0.5846421533961674 1101 92-93 0.7995623147906805 1101 94-95 0.3436445252721043 1101 96-97 0.5560684215318084 1101 98-99 0.8147182374684867 1101 100-101 0.4818054352730421 1102 1 0.48542377990649044 1102 2 0.46609068716335145 1102 3 0.19125950875106668 1102 4 0.1458184465002148 1102 5 0.07480618725193722 1102 6 0.24771181053441182 1102 7 0.10314604780631953 1102 8 -0.02674355241064319 1102 9 0.167600554740595 1102 10-11 0.27475479561291394 1102 12-13 0.36126858635204684 1102 14-15 0.4040169830019522 1102 16-17 0.3813215217031427 1102 18-19 0.40951990555839046 1102 20-21 0.19685697317448358 1102 22-23 0.07761099256877912 1102 24-25 0.018120331790512978 1102 26-27 -0.030258351299110586 1102 28-29 0.07999548980767912 1102 30-31 0.32024458590692717 1102 32-33 0.28715055558009084 1102 34-35 0.3123330945597118 1102 36-37 0.21900821421480288 1102 38-39 0.2199949403212429 1102 40-41 0.22274941414336524 1102 42-43 0.2220451069140239 1102 44-45 0.1907476396975838 1102 46-47 0.0127693614888571 1102 48-49 0.5384297410134238 1102 50-51 0.33262486149397574 1102 52-53 0.03587036392891463 1102 54-55 0.20446460201839045 1102 56-57 0.2940293395001987 1102 58-59 0.5058577564484423 1102 60-61 0.614855454907044 1102 62-63 0.19717603352881952 1102 64-65 0.23763582382856896 1102 66-67 0.012967977538274056 1102 68-69 -0.13792980109130326 1102 70-71 0.01811760699581555 1102 72-73 0.23352744403155512 1102 74-75 -0.1374379167101445 1102 76-77 0.1053328664806088 1102 78-79 0.37076675582245855 1102 80-81 0.39559945357923 1102 82-83 0.42479817136636555 1102 84-85 0.3719253144301362 1102 86-87 0.6323620828996894 1102 88-89 0.502356225030848 1102 90-91 0.34148175033070416 1102 92-93 0.6120169774432291 1102 94-95 0.09397903502738814 1102 96-97 0.06676051845694886 1102 98-99 -0.003935191632358226 1102 100-101 -0.3034891024457522 1103 1 0.4098701529530615 1103 2 0.4961069525594368 1103 3 0.2167007722792249 1103 4 0.2639523515172044 1103 5 -0.13276440629132935 1103 6 0.005311428901087822 1103 7 0.13779478996818284 1103 8 0.02937206409384885 1103 9 -0.10879335521922684 1103 10-11 -0.17048743234403219 1103 12-13 -7.126308513321078E-4 1103 14-15 -0.13056997364473233 1103 16-17 -0.017406271433209497 1103 18-19 0.20210449423928623 1103 20-21 0.12378626180767327 1103 22-23 -0.06883792086827611 1103 24-25 0.027813473310956738 1103 26-27 0.0845348252810183 1103 28-29 -0.07794409257971324 1103 30-31 -0.2715744371347526 1103 32-33 -0.14766572590896487 1103 34-35 -0.1083472358060007 1103 36-37 0.13307318383932198 1103 38-39 -0.0719028233304897 1103 40-41 -0.10695102668909584 1103 42-43 0.14437352997524755 1103 44-45 0.006529097146049878 1103 46-47 0.3440575463741098 1103 48-49 0.2875058320306678 1103 50-51 -0.07561215348972183 1103 52-53 -0.19799212163051294 1103 54-55 0.25215870911711846 1103 56-57 0.1760750435389582 1103 58-59 0.019111467889970868 1103 60-61 0.2548643060413127 1103 62-63 -0.07233525741485636 1103 64-65 -0.2925902249446324 1103 66-67 0.18867448771995043 1103 68-69 0.09042658925521607 1103 70-71 0.12092295675930487 1103 72-73 -0.0910620415106358 1103 74-75 0.10542657472322148 1103 76-77 -0.03763737876478146 1103 78-79 -0.17674343160318173 1103 80-81 -0.16268727719110387 1103 82-83 -0.02271431505519317 1103 84-85 -0.03795260888685448 1103 86-87 -0.28388118535290374 1103 88-89 0.05918021965119635 1103 90-91 -0.08751950836955658 1103 92-93 -0.24273677135698435 1103 94-95 -0.4986144622626725 1103 96-97 -0.7898712226301328 1103 98-99 -0.43910350687067634 1103 100-101 -0.5211178031107373 1104 1 0.16610669722610893 1104 2 0.18488216044424988 1104 3 -0.11127331371891813 1104 4 -0.07834159068983126 1104 5 -0.16686541559430168 1104 6 0.013212039223240879 1104 7 -0.05150383335047337 1104 8 -0.043876469355360825 1104 9 -0.02222241761430155 1104 10-11 -0.19998633613435146 1104 12-13 -0.03629006670825419 1104 14-15 -0.012850879844741314 1104 16-17 -0.15695573912958594 1104 18-19 -0.12923722690867834 1104 20-21 -0.5166915276015516 1104 22-23 -0.190408562200318 1104 24-25 -0.6160862679440555 1104 26-27 -0.20936294082141416 1104 28-29 -0.1933720093862874 1104 30-31 -0.15348994010735595 1104 32-33 -0.22879018027306586 1104 34-35 0.037917750664199446 1104 36-37 -0.17361863310353698 1104 38-39 -0.05983957894486025 1104 40-41 0.45925114992903815 1104 42-43 0.7113904570328629 1104 44-45 0.4380258677715787 1104 46-47 0.5367929351828948 1104 48-49 0.5999601566272474 1104 50-51 0.35794371370101175 1104 52-53 0.30702970380549743 1104 54-55 0.5197721322540545 1104 56-57 0.6294071259423291 1104 58-59 0.7335587214403105 1104 60-61 0.6954270270115117 1104 62-63 0.5317552835794359 1104 64-65 0.5547897351572857 1104 66-67 0.5853048774175491 1104 68-69 0.49423172070162025 1104 70-71 0.8738955613930095 1104 72-73 1.0113693713160075 1104 74-75 0.886550041511029 1104 76-77 0.7136020294795031 1104 78-79 0.6917015307701604 1104 80-81 0.5790022683262848 1104 82-83 0.507767227143944 1104 84-85 0.5691994186072691 1104 86-87 0.44904362058835545 1104 88-89 0.5404854004764168 1104 90-91 0.3400121434505472 1104 92-93 0.08600268626611651 1104 94-95 -0.031925410533425236 1104 96-97 -0.08877600052422352 1104 98-99 -0.10035002566425533 1104 100-101 -0.8076186227990583 1105 1 0.2325952536425291 1105 2 0.3205932656183528 1105 3 -0.030135189219855363 1105 4 -0.027972011920574857 1105 5 0.05683215320078716 1105 6 0.10217810922056714 1105 7 -0.14418350206540254 1105 8 -0.059514549326863175 1105 9 0.08328118911826721 1105 10-11 0.007507652644711982 1105 12-13 -0.03543513750707916 1105 14-15 0.196076896448794 1105 16-17 0.25591272645061025 1105 18-19 0.0579922681487588 1105 20-21 0.03173219668377669 1105 22-23 0.2838277901017534 1105 24-25 0.21212639569473168 1105 26-27 0.13875354328224176 1105 28-29 -0.1577276955560265 1105 30-31 0.28939900815161224 1105 32-33 0.1899648290777236 1105 34-35 0.11909149721331147 1105 36-37 -0.18232377158425805 1105 38-39 -0.13760696695803887 1105 40-41 -0.013569225883586 1105 42-43 0.2181942685922138 1105 44-45 0.04482522657467314 1105 46-47 0.43580442329403724 1105 48-49 0.38069308030700455 1105 50-51 -0.12148900515195038 1105 52-53 -0.012068167777009364 1105 54-55 -0.14054045123512537 1105 56-57 0.12820488175317024 1105 58-59 0.39373772224180215 1105 60-61 0.7151482844698762 1105 62-63 0.25432185228546444 1105 64-65 0.228687677983892 1105 66-67 0.21886084856369337 1105 68-69 0.1705207936377633 1105 70-71 -0.015591481086278947 1105 72-73 0.4147267495331235 1105 74-75 0.6876676833313162 1105 76-77 0.06816165188577372 1105 78-79 0.7547570121131102 1105 80-81 0.5091162588864435 1105 82-83 0.6392660248997544 1105 84-85 0.5663719183846325 1105 86-87 -0.08439657889512375 1105 88-89 0.09380715664348571 1105 90-91 0.03550595411934765 1105 92-93 0.10794586909628734 1105 94-95 0.42084420385788235 1105 96-97 0.5408749033852942 1105 98-99 0.7401032649226202 1105 100-101 0.8127616425852153 1106 1 0.16863358736229017 1106 2 0.19365919034205348 1106 3 -0.040151419222173246 1106 4 -0.10043510798673339 1106 5 -0.22135008653953747 1106 6 -0.5463264119943645 1106 7 -0.06989646288154461 1106 8 0.022918405306420198 1106 9 0.0565248710102253 1106 10-11 -0.2735959875129552 1106 12-13 -0.22154689266590566 1106 14-15 -0.5827931862469384 1106 16-17 -0.10714469684331362 1106 18-19 -0.2643587636156681 1106 20-21 -0.1370598274293684 1106 22-23 -0.4343652696401108 1106 24-25 -0.5048919210221641 1106 26-27 -0.34420495428325637 1106 28-29 -0.5145945322512873 1106 30-31 -0.34258182498850687 1106 32-33 -0.19222853980704002 1106 34-35 -0.6676771866107813 1106 36-37 -0.45154859575922757 1106 38-39 -0.38109564459927725 1106 40-41 -0.5193038267028101 1106 42-43 -0.22742749506803506 1106 44-45 -0.14819587242833876 1106 46-47 -0.3057907540767033 1106 48-49 -0.5312089213932794 1106 50-51 -0.17602954151488603 1106 52-53 0.20671999204986946 1106 54-55 -0.32004427687852655 1106 56-57 -0.5594835022023048 1106 58-59 -0.5359631820011828 1106 60-61 -0.09196200226016771 1106 62-63 -0.4412651327983994 1106 64-65 -0.42048922904137953 1106 66-67 -0.19869073965796247 1106 68-69 -0.010235060755931613 1106 70-71 0.1497458711047699 1106 72-73 0.488627360090355 1106 74-75 0.3606717794747567 1106 76-77 0.1209400737112638 1106 78-79 0.21110603444868659 1106 80-81 0.12720884718538628 1106 82-83 0.28621710303562153 1106 84-85 0.6043578523826234 1106 86-87 0.16025138462601163 1106 88-89 -0.012808501444467879 1106 90-91 -0.1762337475577489 1106 92-93 0.3751303501226424 1106 94-95 -0.18928022615989448 1106 96-97 -0.0665986262537821 1106 98-99 -0.25191311965115304 1106 100-101 -0.36294745394465266 1107 1 1.0074490548121204 1107 2 0.9012950200043193 1107 3 0.6102156879731311 1107 4 0.22115079509696756 1107 5 0.33753390758674584 1107 6 0.21270442501003828 1107 7 0.2815474920866379 1107 8 0.48376030447430907 1107 9 0.49029873297791937 1107 10-11 0.568033968434186 1107 12-13 0.28415550576777804 1107 14-15 0.4162523350452787 1107 16-17 0.5921700363921261 1107 18-19 0.18167647867507952 1107 20-21 0.26974389259020626 1107 22-23 0.4969856848385916 1107 24-25 0.541950957098237 1107 26-27 0.7647769350951066 1107 28-29 0.41215534537964515 1107 30-31 0.5879370198475158 1107 32-33 0.6423040103642776 1107 34-35 0.48224939195015537 1107 36-37 0.7372668716906006 1107 38-39 0.20654507982558812 1107 40-41 0.11040738230354918 1107 42-43 0.3965568416916341 1107 44-45 0.2606147002588912 1107 46-47 0.1945763531185989 1107 48-49 0.29355857738303115 1107 50-51 0.3481016381229054 1107 52-53 0.406352884854563 1107 54-55 -0.3820609190713853 1107 56-57 -0.08875418257431278 1107 58-59 -0.15304590348919334 1107 60-61 -0.39303885003305084 1107 62-63 -1.2085436447905593 1107 64-65 -0.9637099828348177 1107 66-67 -0.5969286251205119 1107 68-69 -0.28678914788270404 1107 70-71 -0.18050376178803518 1107 72-73 0.08928815304189186 1107 74-75 0.03796008099213566 1107 76-77 0.031904341944255066 1107 78-79 0.22464005304878398 1107 80-81 0.30765427058234707 1107 82-83 0.08692684361320602 1107 84-85 0.4880093452852172 1107 86-87 -0.3472620759711482 1107 88-89 0.4955615426083959 1107 90-91 0.43375156815443816 1107 92-93 0.13484645389161187 1107 94-95 0.74131204011519 1107 96-97 0.4540327981221388 1107 98-99 0.06671145205712037 1107 100-101 0.9876154437548088 1108 1 0.19675331234067528 1108 2 0.3065909701600802 1108 3 0.3332686474746822 1108 4 -0.10595200864135279 1108 5 -0.15654708929798744 1108 6 -0.3508469768591169 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-0.4469195828302439 2316 98-99 -0.9078917225460508 2316 100-101 -1.4741305879912225 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 4.0 5 12.0 6 28.0 7 61.0 8 147.0 9 225.0 10 370.0 11 591.0 12 807.0 13 1069.0 14 1493.0 15 1986.0 16 2416.0 17 2972.0 18 2711.0 19 1939.0 20 1817.0 21 1852.0 22 1988.0 23 2283.0 24 2664.0 25 3056.0 26 3698.0 27 4506.0 28 5644.0 29 7018.0 30 8683.0 31 10752.0 32 13380.0 33 17365.0 34 23027.0 35 32013.0 36 45413.0 37 60218.0 38 38871.0 39 2379.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.331283551330415 19.66427894763084 14.456946268011766 22.547491233026975 2 16.14118344950319 20.98077018142271 39.070311598859725 23.807734770214378 3 20.40079222538219 23.973715517269788 29.917383151699294 25.708109105648724 4 13.526687869749354 17.09770446418272 36.782624458250254 32.592983207817674 5 13.870691359652012 37.82343636723127 33.98470968167139 14.32116259144533 6 32.667237856719176 36.66578791273973 16.52507744018981 14.141896790351282 7 29.129374546892507 32.92328478217887 20.038225795821525 17.9091148751071 8 26.81144137612865 34.99505700916101 19.003163514136954 19.19033810057339 9 26.58524448076879 17.096681705383947 19.737080599395597 36.58099321445167 10-11 25.23693558910953 26.659208193555617 26.99368611142234 21.110170105912516 12-13 26.433554450518855 23.875218728065406 27.36926569981447 22.321961121601273 14-15 22.759214985854783 26.00758915110583 25.737536639979965 25.49565922305942 16-17 22.392737098793912 28.884202201278587 26.53595202003559 22.187108679891914 18-19 22.186815703578237 27.503175876846832 29.057749057122944 21.25225936245199 20-21 22.855071508600805 26.312364067751925 29.211592961181044 21.620971462466223 22-23 22.313997841542886 26.21388497565557 29.208703030902182 22.26341415189936 24-25 22.901370856126015 26.541883609042376 29.128056416002106 21.4286891188295 26-27 22.4476042971067 26.820173993277535 29.080603703947798 21.651618005667963 28-29 21.94885652145258 27.445297568048506 28.967573980096223 21.638271930402688 30-31 22.272659119256442 26.951042890402178 28.783412613544808 21.992885376796576 32-33 22.236649573550928 26.96614772143055 28.75400413925836 22.04319856576016 34-35 22.698108793008565 26.950441083909748 28.67737438912268 21.67407573395901 36-37 23.03593319536533 26.71691074700447 28.643080289469175 21.604075768161028 38-39 22.413040290384714 26.878710338611906 28.88260212647816 21.825647244525218 40-41 22.560138674452823 26.99488703865122 28.66405553569104 21.78091875120492 42-43 22.5189594312665 27.30289936752458 28.20662401824588 21.971517182963044 44-45 22.752969594711494 27.092557105689398 28.24628728573523 21.908186013863876 46-47 23.31553797989784 27.403361344537814 27.670621189652334 21.610479485912013 48-49 23.211837369649192 27.490672748605853 27.205911433957787 22.091578447787168 50-51 22.68811056536561 27.759809001889877 27.71779337207559 21.83428706066892 52-53 23.020647451075376 27.6604333806226 27.466497941186645 21.85242122711537 54-55 22.59382853376179 27.83141762452107 27.31627734519837 22.258476496518767 56-57 22.973367101622888 27.63853210102145 27.39746342363659 21.990637373719064 58-59 23.134846030775723 27.167259563656376 27.122442153410653 22.57545225215725 60-61 22.584068404912525 27.470722097627615 27.882680763342933 22.062528734116935 62-63 22.307723995880536 27.599093717816686 28.078599382080327 22.01458290422245 64-65 22.167230319930912 27.920778698521314 27.64406120417433 22.26792977737344 66-67 22.289797855302098 27.477376534869467 27.47852995261101 22.75429565721743 68-69 22.412509062149873 27.286462795755618 27.480722335727936 22.820305806366573 70-71 22.712637996375022 27.454934915142527 27.37057175811501 22.46185533036744 72-73 22.39339616423911 27.148553351347786 27.700520661701706 22.757529822711394 74-75 22.134712976998618 27.61698411652277 27.19765372701509 23.05064917946352 76-77 22.14752604617212 27.306340551949816 27.40207248934344 23.144060912534623 78-79 22.467967996835938 27.27470481118627 27.078598914001795 23.178728277975992 80-81 22.513724061893832 27.421260718447016 26.97332315942655 23.091692060232603 82-83 22.277099637940896 27.543848373535575 26.98897672571345 23.190075262810087 84-85 22.012411756563466 27.394190269657443 27.316575594386695 23.2768223793924 86-87 22.025842961410238 27.431243912929553 26.86698999207342 23.675923133586785 88-89 21.630513615085384 27.361376673040155 26.668095206698755 24.34001450517571 90-91 21.819083848679437 27.280653753345902 26.96666622713907 23.933596170835585 92-93 21.599746908860826 27.381297619361472 27.208121325564843 23.810834146212862 94-95 21.309770795878315 27.696473034857682 26.889174888232297 24.104581281031706 96-97 21.39205753328038 27.962275095828375 26.877263873279126 23.768403497612116 98-99 21.103501237611525 28.43111727811158 25.93974430385588 24.52563718042102 100-101 20.64817225706724 28.18441894880474 25.918485730388554 25.248923063739465 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 39.0 1 25.0 2 17.5 3 27.5 4 35.0 5 41.5 6 54.0 7 88.5 8 134.5 9 171.0 10 180.5 11 179.0 12 191.5 13 202.5 14 233.5 15 260.5 16 291.5 17 321.0 18 329.0 19 384.0 20 435.0 21 454.0 22 545.5 23 683.0 24 837.5 25 1080.5 26 1381.5 27 1748.0 28 2213.0 29 2803.0 30 3437.0 31 4145.5 32 5021.0 33 5584.0 34 5959.5 35 6524.5 36 7383.0 37 8121.5 38 8426.0 39 8720.0 40 9203.0 41 9772.5 42 10034.5 43 10361.0 44 10706.0 45 11061.0 46 11439.5 47 12119.0 48 13201.5 49 12887.0 50 11743.0 51 11022.5 52 10715.0 53 10813.5 54 10586.0 55 9569.0 56 8326.0 57 7531.5 58 7014.0 59 6098.0 60 4762.5 61 3784.0 62 3032.0 63 2246.5 64 1592.0 65 1150.5 66 894.0 67 713.0 68 560.0 69 407.0 70 310.5 71 242.0 72 182.0 73 147.0 74 120.5 75 105.5 76 79.0 77 52.0 78 36.5 79 33.5 80 27.5 81 21.5 82 24.0 83 18.5 84 10.0 85 5.0 86 3.0 87 3.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.8617280695973111 2 0.008238318064983853 3 0.0036248599485928954 4 0.014828972516970934 5 0.0 6 0.0 7 0.0 8 0.0 9 0.006920187174586437 10-11 6.590654451987082E-4 12-13 3.295327225993541E-4 14-15 1.6476636129967705E-4 16-17 0.0 18-19 1.6476636129967705E-4 20-21 0.0 22-23 8.238318064983852E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 1.6476636129967705E-4 32-33 0.007908785342384499 34-35 9.885981677980624E-4 36-37 0.002636261780794833 38-39 1.6476636129967705E-4 40-41 0.005107757200289989 42-43 0.01614710340736835 44-45 0.003130560864693864 46-47 0.003295327225993541 48-49 0.015817570684768998 50-51 4.942990838990312E-4 52-53 0.00280102814209451 54-55 0.01565280432346932 56-57 0.006425888090687405 58-59 0.0021419626968958018 60-61 0.010380280761879655 62-63 0.007414486258485467 64-65 0.02702168325314704 66-67 0.004613458116390958 68-69 0.0 70-71 0.003295327225993541 72-73 0.0 74-75 0.0 76-77 0.00280102814209451 78-79 0.017300467936466092 80-81 0.022902524220655113 82-83 0.01861859882686351 84-85 0.012522243458775456 86-87 0.01664140249126738 88-89 0.03954392671192249 90-91 0.03427140315033283 92-93 0.0039543926711922495 94-95 0.01334607526527384 96-97 0.01746523429776577 98-99 0.01746523429776577 100-101 0.01746523429776577 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 303460.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.93600454113215 #Duplication Level Percentage of deduplicated Percentage of total 1 89.34330979594165 68.73717288180345 2 6.851437717132729 10.542444866372156 3 1.6760731999552616 3.868511259690837 4 0.7207212749286835 2.2179766112321504 5 0.37528675384033344 1.443653169884332 6 0.24966832744372905 1.1525090144392565 7 0.15738902674850855 0.8476218013653338 8 0.10251901408495165 0.6309922666553786 9 0.07482247936355209 0.518088835088371 >10 0.40960861630046597 5.538588261632286 >50 0.022379311005812016 1.1777601669549607 >100 0.014632626426858709 2.0797052596674455 >500 0.0017214854619833776 0.8920117767145516 >1k 4.303713654958444E-4 0.3529638284995219 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1066 0.3512818822909115 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 863 0.2843867396032426 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 790 0.2603308508534898 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 532 0.1753114084228564 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 509 0.16773215580307124 No Hit CCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAA 437 0.14400579977591776 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 425 0.1400514071047255 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.295327225993541E-4 0.0 22-23 0.0 0.0 0.0 6.590654451987082E-4 3.295327225993541E-4 24-25 0.0 0.0 0.0 6.590654451987082E-4 3.295327225993541E-4 26-27 0.0 0.0 0.0 0.002471495419495156 3.295327225993541E-4 28-29 0.0 0.0 0.0 0.006261121729387729 3.295327225993541E-4 30-31 0.0 0.0 0.0 0.013346075265273842 3.295327225993541E-4 32-33 0.0 0.0 0.0 0.019277664272062213 3.295327225993541E-4 34-35 0.0 0.0 0.0 0.028998879588743164 3.295327225993541E-4 36-37 0.0 0.0 0.0 0.04250972121531668 3.295327225993541E-4 38-39 0.0 0.0 0.0 0.06310551637777631 3.295327225993541E-4 40-41 0.0 0.0 0.0 0.10363804125749687 3.295327225993541E-4 42-43 0.0 0.0 0.0 0.16328346404797994 3.295327225993541E-4 44-45 0.0 0.0 0.0 0.2384169248006327 3.295327225993541E-4 46-47 0.0 0.0 0.0 0.3845646872734463 3.295327225993541E-4 48-49 0.0 0.0 0.0 0.5157187108679893 3.295327225993541E-4 50-51 0.0 0.0 0.0 0.6051868450537139 3.295327225993541E-4 52-53 0.0 0.0 0.0 0.7073419890595136 3.295327225993541E-4 54-55 0.0 0.0 0.0 0.8045541422263232 3.295327225993541E-4 56-57 0.0 0.0 0.0 1.0609306004086205 3.295327225993541E-4 58-59 0.0 0.0 0.0 1.3444935082053648 3.295327225993541E-4 60-61 0.0 0.0 0.0 1.6189942661306267 3.295327225993541E-4 62-63 0.0 0.0 0.0 1.9481974560073816 3.295327225993541E-4 64-65 0.0 0.0 0.0 2.3233704606867462 3.295327225993541E-4 66-67 0.0 0.0 0.0 2.815362815527582 3.295327225993541E-4 68-69 0.0 0.0 0.0 3.3543135833388256 3.295327225993541E-4 70-71 0.0 0.0 0.0 3.7942397680089632 3.295327225993541E-4 72-73 0.0 0.0 0.0 4.180616885256706 6.590654451987082E-4 74-75 0.0 0.0 0.0 4.599123442957886 6.590654451987082E-4 76-77 0.0 0.0 0.0 5.153397482369999 6.590654451987082E-4 78-79 0.0 0.0 0.0 5.631384696500362 6.590654451987082E-4 80-81 0.0 0.0 0.0 6.238383971528373 6.590654451987082E-4 82-83 0.0 0.0 0.0 7.048540170038885 6.590654451987082E-4 84-85 0.0 0.0 0.0 8.184933763922757 6.590654451987082E-4 86-87 0.0 0.0 0.0 9.342911751136889 6.590654451987082E-4 88-89 0.0 0.0 0.0 10.425426744875766 6.590654451987082E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 180 0.0 36.93009 2 TAAGGTG 205 0.0 34.74832 5 GTAAGGT 220 0.0 32.379116 4 GGTATAT 60 0.0037938545 31.922623 1 CCTAGAC 90 2.4108404E-5 31.659582 3 GGTATAC 60 0.0039539244 31.654364 2 GATAGGG 80 4.4918162E-4 29.680857 7 TATAGGT 65 0.00584377 29.224228 3 GTATAGG 100 4.6772613E-5 28.73036 1 GGTAAGG 235 0.0 28.29154 3 AAGGTAA 240 0.0 27.932295 1 TACTACC 70 0.008391163 27.136784 4 GGTGTGC 320 0.0 26.712772 8 CCATAAC 90 8.943992E-4 26.382986 3 AAGTCGG 65 1.0041131E-5 25.566988 66-67 AAGGTGT 285 0.0 24.994406 6 GTATAAT 120 1.6160608E-4 23.941967 1 GTACATG 1040 0.0 23.481543 1 GTATGGT 105 0.0021953778 22.613985 3 AGGTGTG 360 0.0 22.425537 7 >>END_MODULE