FastQCFastQC Report
Thu 26 May 2016
SRR936545_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936545_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349224
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9230.26430027718598953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7790.22306599775502256No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT5380.15405584954069595No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4770.136588550615078No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4230.12112569582846541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.11768950587588481No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT3810.10909903099443337No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC905.671627E-736.953753
GTATAGG856.383406E-427.9522171
CGCCGGG350.00840250527.13414460-61
GGTATCA12500.025.089911
GTATCAA18200.024.8037551
CCTACAC1601.8493265E-623.7559813
CGACAGC1050.002184506322.6344748
AGGCGAC1050.002186327222.631235
GGCGACA1050.002186327222.631236
ACATGGG11150.022.5841623
GTACATG11850.022.456131
TACATGG11850.021.6510222
GAGTACT11150.021.3180312-13
CAACGCA20900.021.1477475
TGGACCC1805.1277802E-621.122485
TATACTG1805.1277802E-621.122485
ATCAACG21200.021.0666243
TCAACGC21000.021.0470434
TATCAAC21300.020.9677222
GCGACAG1150.003703501820.6662587
Produced by FastQC (version 0.11.5)