##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936545_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 349224 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.510274207958215 31.0 31.0 34.0 30.0 34.0 2 31.597759604150916 33.0 31.0 34.0 30.0 34.0 3 31.630495040432503 33.0 31.0 34.0 30.0 34.0 4 35.25238242503379 37.0 35.0 37.0 33.0 37.0 5 35.214730373628385 37.0 35.0 37.0 33.0 37.0 6 35.31474354568987 37.0 35.0 37.0 33.0 37.0 7 35.17728449362014 37.0 35.0 37.0 32.0 37.0 8 35.12671523148466 37.0 35.0 37.0 32.0 37.0 9 36.66215953084553 39.0 37.0 39.0 32.0 39.0 10-11 36.56427822830046 39.0 37.0 39.0 32.0 39.0 12-13 36.48096350766271 39.0 37.0 39.0 32.0 39.0 14-15 37.589305145121756 40.0 37.0 41.0 32.0 41.0 16-17 37.457007536709966 40.0 37.0 41.0 32.0 41.0 18-19 37.428345417268 40.0 37.0 41.0 32.0 41.0 20-21 37.339495567314955 40.0 37.0 41.0 31.5 41.0 22-23 37.194738906833436 39.0 37.0 41.0 31.0 41.0 24-25 37.29473346619935 40.0 37.0 41.0 31.5 41.0 26-27 37.27488087874831 40.0 37.0 41.0 31.0 41.0 28-29 37.16185313724142 39.5 37.0 41.0 31.0 41.0 30-31 36.99004793484984 39.0 36.0 41.0 31.0 41.0 32-33 36.830119063981854 39.0 36.0 41.0 30.0 41.0 34-35 36.597938572377615 39.0 36.0 41.0 30.0 41.0 36-37 36.389178578791835 39.0 35.0 40.5 30.0 41.0 38-39 36.19538319244954 39.0 35.0 40.0 30.0 41.0 40-41 35.99538261975122 39.0 35.0 40.0 29.0 41.0 42-43 35.78438910269627 38.0 35.0 40.0 28.5 41.0 44-45 35.513256534487894 38.0 35.0 40.0 27.5 41.0 46-47 35.284158591620276 38.0 35.0 40.0 27.0 41.0 48-49 35.241116017226766 38.5 35.0 40.0 26.0 41.0 50-51 34.345337090234345 37.5 34.0 39.5 25.0 40.5 52-53 34.50431957711956 38.0 34.0 39.5 25.5 40.5 54-55 34.90953227727762 38.0 34.0 40.0 26.0 41.0 56-57 34.80555746454998 38.0 34.0 40.0 25.5 41.0 58-59 34.535508727922476 38.0 34.0 40.0 24.5 41.0 60-61 34.195251471834695 38.0 34.0 40.0 23.5 41.0 62-63 33.88980568345818 37.0 33.0 40.0 23.0 41.0 64-65 33.54847748150184 37.0 33.0 40.0 22.0 41.0 66-67 33.16618416832749 36.0 32.5 39.0 21.5 41.0 68-69 32.71887241426706 36.0 32.0 39.0 19.5 41.0 70-71 32.27265880924564 35.0 31.5 39.0 18.5 40.0 72-73 31.888737601081253 35.0 31.0 38.0 17.5 40.0 74-75 31.45393501019403 35.0 31.0 37.0 14.0 39.5 76-77 30.996068425995922 35.0 31.0 37.0 10.0 39.0 78-79 30.520053031864933 34.5 30.0 36.0 7.0 39.0 80-81 30.055727842301792 34.0 30.0 36.0 6.0 37.5 82-83 29.582986850846446 34.0 29.0 35.0 2.0 37.0 84-85 29.199631182278424 34.0 29.0 35.0 2.0 37.0 86-87 28.864310585755845 34.0 29.0 35.0 2.0 36.0 88-89 28.420778640643256 34.0 29.0 35.0 2.0 36.0 90-91 27.977173676494168 34.0 27.0 35.0 2.0 35.5 92-93 27.588307218289692 34.0 27.0 35.0 2.0 35.0 94-95 27.110056868943715 33.0 26.0 35.0 2.0 35.0 96-97 26.498377831993217 33.0 25.0 35.0 2.0 35.0 98-99 25.73373250406616 33.0 23.5 35.0 2.0 35.0 100-101 23.388882207408425 30.5 9.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4787499933221824 1101 2 0.4796871630533275 1101 3 0.34149828712970987 1101 4 0.512883631373164 1101 5 0.630001307354874 1101 6 0.3610674405286929 1101 7 0.36451158141073137 1101 8 0.5745592634368393 1101 9 0.6160639405282637 1101 10-11 0.6319445876861423 1101 12-13 0.6935570895058021 1101 14-15 0.7109015478147072 1101 16-17 0.8966763800523054 1101 18-19 1.0173531998918932 1101 20-21 0.7915284922940558 1101 22-23 0.8947136542049137 1101 24-25 0.8522109708517789 1101 26-27 0.6744499066061778 1101 28-29 0.858026529181096 1101 30-31 0.8338424677102765 1101 32-33 0.5502926285096734 1101 34-35 0.5179369304312829 1101 36-37 0.5247746984137152 1101 38-39 0.6415887034663825 1101 40-41 0.7228765832083823 1101 42-43 0.8000031808864279 1101 44-45 0.690700320371171 1101 46-47 0.916162811945533 1101 48-49 0.9316759142112119 1101 50-51 0.7115243731507306 1101 52-53 0.8132524116450384 1101 54-55 0.85443792124385 1101 56-57 0.7004210494765459 1101 58-59 0.6576971418613269 1101 60-61 0.7292777399700157 1101 62-63 0.8046122446810173 1101 64-65 0.8250167830098647 1101 66-67 0.7731626709916242 1101 68-69 0.740122027543606 1101 70-71 0.7194836683830808 1101 72-73 0.846909628318226 1101 74-75 0.6569264867744238 1101 76-77 0.6999911428133281 1101 78-79 0.6583156173566636 1101 80-81 0.5964775049341071 1101 82-83 0.6352975472465658 1101 84-85 0.5502945820439322 1101 86-87 0.46087457800500076 1101 88-89 0.6178743881719164 1101 90-91 0.47010477049625266 1101 92-93 0.661104796636554 1101 94-95 0.4435666544425665 1101 96-97 0.15291698201832205 1101 98-99 0.4308234602207257 1101 100-101 0.6202547477268929 1102 1 0.4559275397773561 1102 2 0.6027043978584814 1102 3 0.5385482884007153 1102 4 0.601526581117696 1102 5 0.5941320046273049 1102 6 0.42680373275777583 1102 7 0.5038755077942128 1102 8 0.6247956700562227 1102 9 1.044141039590265 1102 10-11 0.7635212355419654 1102 12-13 0.5787854888042077 1102 14-15 0.5640585612110769 1102 16-17 0.4306995504430162 1102 18-19 0.5901626256525887 1102 20-21 0.6637616769200037 1102 22-23 0.5735771865773671 1102 24-25 0.5411456158605432 1102 26-27 0.4679412574285138 1102 28-29 0.6401932403328843 1102 30-31 0.4637715774995499 1102 32-33 0.2601178020016235 1102 34-35 0.2247053409145252 1102 36-37 0.245528911789755 1102 38-39 0.29703320510597564 1102 40-41 0.13749614013662637 1102 42-43 0.38419364709045567 1102 44-45 0.24563387867208775 1102 46-47 0.36870354760225865 1102 48-49 0.3801605604909781 1102 50-51 0.5805188824163494 1102 52-53 0.6235257726862429 1102 54-55 0.7436262902923758 1102 56-57 0.7991517937760975 1102 58-59 0.8079289410936497 1102 60-61 0.6765279242654998 1102 62-63 0.6060966176950728 1102 64-65 0.6544457529887708 1102 66-67 0.6856453535712532 1102 68-69 0.5019232934629159 1102 70-71 0.6287300587336269 1102 72-73 0.9039157164241765 1102 74-75 0.7504929856686537 1102 76-77 0.8834414084530238 1102 78-79 0.6514919182285723 1102 80-81 0.6442426044536695 1102 82-83 0.8011070810425416 1102 84-85 0.7356036900537966 1102 86-87 0.7149989838109434 1102 88-89 0.8929409569713265 1102 90-91 0.9483904729797956 1102 92-93 0.9101912629930915 1102 94-95 1.127282242027409 1102 96-97 0.6557958045608352 1102 98-99 0.8722047866397986 1102 100-101 0.931675983650532 1103 1 0.12729540827471908 1103 2 0.17672266987748841 1103 3 0.1447129112564589 1103 4 0.2981123687709797 1103 5 0.4870087995125374 1103 6 0.34651864733696414 1103 7 0.0656374649615259 1103 8 0.30252861209584125 1103 9 0.3327332556402922 1103 10-11 0.5350085446989326 1103 12-13 0.26873762519801403 1103 14-15 0.24739123947681207 1103 16-17 0.25461082808686797 1103 18-19 0.3187462821526097 1103 20-21 0.4476013775104377 1103 22-23 0.3275490798100833 1103 24-25 0.5521332831211438 1103 26-27 0.13144503935214402 1103 28-29 0.14787827509513107 1103 30-31 0.090726954294432 1103 32-33 0.47480310752515464 1103 34-35 0.0784833341367559 1103 36-37 0.27813380994857795 1103 38-39 0.07798187400860712 1103 40-41 0.05312889176992286 1103 42-43 0.2672389690343451 1103 44-45 0.4935147349764648 1103 46-47 0.6386263369674552 1103 48-49 0.6778341970769475 1103 50-51 0.5813267991266855 1103 52-53 0.5926251420745032 1103 54-55 0.5996457064961263 1103 56-57 0.7878638871974672 1103 58-59 0.2466113381170203 1103 60-61 0.23644914146844087 1103 62-63 0.23729464899856367 1103 64-65 0.5846144547321188 1103 66-67 0.48211912466443607 1103 68-69 0.5087185280644633 1103 70-71 0.6605587017829215 1103 72-73 0.7375700328829673 1103 74-75 0.6235649033572237 1103 76-77 0.5710265672414074 1103 78-79 0.44552774605934076 1103 80-81 0.4348164000027026 1103 82-83 0.1754509216602287 1103 84-85 0.6416276917307719 1103 86-87 0.7577093915250188 1103 88-89 0.6685420906749933 1103 90-91 0.7032150458757194 1103 92-93 0.7353995744908417 1103 94-95 0.8583573389774131 1103 96-97 0.5154803240032457 1103 98-99 0.3683966603078268 1103 100-101 0.42559695629491046 1104 1 0.11871467967567995 1104 2 0.08681755922169287 1104 3 0.07757107130412422 1104 4 -0.04922186550253116 1104 5 0.08953070543694253 1104 6 -0.07107802209065284 1104 7 0.104715745037673 1104 8 0.01728296058531953 1104 9 -0.047343614900412945 1104 10-11 0.3938933406069225 1104 12-13 0.01933340821674534 1104 14-15 0.053002498322733516 1104 16-17 0.04999205656238814 1104 18-19 0.36475780546806646 1104 20-21 0.7222664312035647 1104 22-23 0.2996998565077007 1104 24-25 0.2949584571333759 1104 26-27 -0.2665543648648381 1104 28-29 0.21091320832383786 1104 30-31 0.1486414231030082 1104 32-33 -0.3297546325681324 1104 34-35 -0.3184480200344453 1104 36-37 -0.09969797580203732 1104 38-39 0.10421994018411596 1104 40-41 0.2068863208935383 1104 42-43 0.07999599128175561 1104 44-45 0.38192530213283504 1104 46-47 0.10088675227181199 1104 48-49 0.05297406077341549 1104 50-51 0.006521518454754016 1104 52-53 0.09098128953805684 1104 54-55 0.1862394987276943 1104 56-57 0.20990894873749255 1104 58-59 -0.011873120664972703 1104 60-61 -0.3277791742093683 1104 62-63 -0.025534666432214692 1104 64-65 0.14194222258017675 1104 66-67 0.16864643622988496 1104 68-69 0.08211064047855388 1104 70-71 -0.018152590484977082 1104 72-73 -0.29365771607441005 1104 74-75 -0.46301435758120846 1104 76-77 -0.2517261682745229 1104 78-79 -0.15481809871738506 1104 80-81 -0.41194384819178254 1104 82-83 -0.5606244666885836 1104 84-85 -0.5364260595225545 1104 86-87 -0.588992731496905 1104 88-89 -0.5412934150866668 1104 90-91 -0.07392759329774279 1104 92-93 -0.11490994205303551 1104 94-95 -0.8089232748244122 1104 96-97 -0.3637155868908657 1104 98-99 -0.36976941784146433 1104 100-101 0.13893450370325766 1105 1 0.0721089507515984 1105 2 -0.2286225898234875 1105 3 0.19168378672098285 1105 4 0.31681213542900366 1105 5 0.37268743575832275 1105 6 0.19598066444870454 1105 7 0.1496621819263524 1105 8 0.40643060846608137 1105 9 0.31254809446148357 1105 10-11 0.13593164987566553 1105 12-13 -0.26986372732120856 1105 14-15 -0.3454459180722367 1105 16-17 -0.3680476500049181 1105 18-19 -0.3277055154448405 1105 20-21 0.03967153134329493 1105 22-23 -0.4779695659422245 1105 24-25 -0.5266385386747245 1105 26-27 -0.1273484962109066 1105 28-29 -0.17440642372087467 1105 30-31 -0.5206209197009031 1105 32-33 -0.35004457201852546 1105 34-35 -0.12733833209779988 1105 36-37 -0.09713626178248091 1105 38-39 0.10095957688648838 1105 40-41 -0.39389383746696893 1105 42-43 -0.20458714798233046 1105 44-45 -0.33852765548244435 1105 46-47 -0.11061476146829108 1105 48-49 -0.38646177993454955 1105 50-51 0.16181087569741948 1105 52-53 -0.528909837616105 1105 54-55 -0.3016214700659745 1105 56-57 0.07431276802021358 1105 58-59 -0.06115700515495348 1105 60-61 0.30222781228341944 1105 62-63 -0.023802598430350486 1105 64-65 -0.10577261207281907 1105 66-67 -0.13420347061687465 1105 68-69 0.18935330466113243 1105 70-71 -0.06720359188227931 1105 72-73 0.14828161787994532 1105 74-75 0.39187036528791097 1105 76-77 -0.09479207418975477 1105 78-79 -0.25412818255529857 1105 80-81 -0.4251162981219174 1105 82-83 -0.18083580809519262 1105 84-85 -0.482586399531872 1105 86-87 -0.36926753354627806 1105 88-89 -0.464281887373577 1105 90-91 -0.37513276330239975 1105 92-93 -0.46606853543915605 1105 94-95 0.006691536540284204 1105 96-97 0.09567542382175631 1105 98-99 0.19625293893543372 1105 100-101 0.06892402396408315 1106 1 -0.3405248583474183 1106 2 -0.1826814658270557 1106 3 0.010149442385568364 1106 4 0.1489932237430054 1106 5 0.354567453599536 1106 6 0.4159851247520052 1106 7 0.1099654825507983 1106 8 -0.10293602846522987 1106 9 0.3171756591358843 1106 10-11 -0.012680080722013543 1106 12-13 0.2659023297706682 1106 14-15 0.404247436075849 1106 16-17 0.23350788077117812 1106 18-19 -0.22754382945019103 1106 20-21 -0.03740808507062354 1106 22-23 -0.14804650617416115 1106 24-25 -0.014344827702380769 1106 26-27 -0.3250514400110873 1106 28-29 0.0016361721756439351 1106 30-31 0.008454682440046213 1106 32-33 -0.18306196720140377 1106 34-35 -0.4789774935607696 1106 36-37 -0.28223884875838934 1106 38-39 -0.23356317066033938 1106 40-41 -0.023577155932620997 1106 42-43 -0.6018886644854504 1106 44-45 -0.44674576431383883 1106 46-47 0.15390910115436895 1106 48-49 0.37360177938748507 1106 50-51 0.062262481406612835 1106 52-53 0.2697910990475876 1106 54-55 0.11669922574134972 1106 56-57 0.027368075781140533 1106 58-59 -0.14082917286236096 1106 60-61 0.04174609952694652 1106 62-63 0.12637023832255068 1106 64-65 0.2785103941715974 1106 66-67 0.3185451912921309 1106 68-69 0.203960797970673 1106 70-71 0.2586773180195969 1106 72-73 -0.08736178087116642 1106 74-75 -0.34513900134902187 1106 76-77 -0.17903605278029744 1106 78-79 0.11175607257404607 1106 80-81 0.38618499536604034 1106 82-83 0.5583758332964202 1106 84-85 0.15955789127990627 1106 86-87 0.7647645806374861 1106 88-89 0.912808879798586 1106 90-91 0.559022009222133 1106 92-93 0.6369087170140126 1106 94-95 0.9656567461745418 1106 96-97 1.3776223462124797 1106 98-99 1.8072029835384669 1106 100-101 2.3272313388614982 1107 1 0.0870542763944755 1107 2 0.25221134432695536 1107 3 0.22569003521893904 1107 4 0.11130264251864475 1107 5 0.23640709449420427 1107 6 0.42155744887552515 1107 7 0.27997941336110443 1107 8 0.4497495221887462 1107 9 0.2628454989315685 1107 10-11 0.39065557441303866 1107 12-13 0.2885882673915887 1107 14-15 -0.3829594364572344 1107 16-17 -0.06401166105994349 1107 18-19 0.5440876232237528 1107 20-21 0.23150492475834028 1107 22-23 0.964164236028445 1107 24-25 0.49334614048893144 1107 26-27 0.5623895475285821 1107 28-29 0.3230025524867699 1107 30-31 0.4180882247355342 1107 32-33 0.7105027723698427 1107 34-35 0.6427139389908874 1107 36-37 0.4583191575878658 1107 38-39 0.33128281649235447 1107 40-41 0.43655557373227083 1107 42-43 0.7767875215682594 1107 44-45 0.8341922435802829 1107 46-47 1.0744744598727394 1107 48-49 0.4387379917549481 1107 50-51 0.22235070987189687 1107 52-53 0.7826964469308777 1107 54-55 1.225340197802332 1107 56-57 0.9648438903465788 1107 58-59 1.8126864219058447 1107 60-61 1.558085779118315 1107 62-63 1.5711790766477591 1107 64-65 1.2029647707678421 1107 66-67 1.055572124192068 1107 68-69 1.6804912522698743 1107 70-71 1.2382067662047227 1107 72-73 0.7920496423932839 1107 74-75 0.7221545517815393 1107 76-77 0.48693407337092864 1107 78-79 0.34774786887464515 1107 80-81 0.5159059921929092 1107 82-83 0.7932164029117743 1107 84-85 -0.0348349575708049 1107 86-87 0.3386752686647263 1107 88-89 0.023694260187102856 1107 90-91 -0.07455898296113261 1107 92-93 -0.2902544282533803 1107 94-95 0.052443759563598036 1107 96-97 0.11759022795537888 1107 98-99 -0.27643992335728385 1107 100-101 0.35123296412269767 1108 1 -0.26961466076337715 1108 2 -0.1468400949121751 1108 3 -0.2423086529417091 1108 4 0.02257689262586382 1108 5 -0.23764623682091468 1108 6 -0.35290709362050166 1108 7 -0.46890426180728895 1108 8 -0.18480230807942633 1108 9 -0.4499373664283084 1108 10-11 -0.248891336457568 1108 12-13 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0.7333361550455137 2311 70-71 0.7917186434814205 2311 72-73 0.26649938842991716 2311 74-75 0.05564180230742721 2311 76-77 0.32134482705211553 2311 78-79 1.2913885235357725 2311 80-81 0.9817620283121471 2311 82-83 0.2640747477888965 2311 84-85 0.9559300108761697 2311 86-87 1.2518146663354486 2311 88-89 1.4592286093739055 2311 90-91 0.670143700328591 2311 92-93 0.37969580518769064 2311 94-95 -0.12658758823795324 2311 96-97 -0.0914549723935032 2311 98-99 0.12728487270243605 2311 100-101 -0.08804670341616472 2312 1 0.26205693116775564 2312 2 0.23129173086194754 2312 3 0.2724671404541539 2312 4 0.18446819418053195 2312 5 0.4827700550973688 2312 6 0.2805889875821208 2312 7 -0.28883231011771215 2312 8 0.10685900503890622 2312 9 0.16345078723994533 2312 10-11 0.5284117600347926 2312 12-13 0.16277856154047754 2312 14-15 -0.011105342796831508 2312 16-17 0.22050039161074864 2312 18-19 0.12888108186238156 2312 20-21 0.04452924248160173 2312 22-23 -0.15548639580759271 2312 24-25 -0.3976223208044729 2312 26-27 -0.347666733665001 2312 28-29 -1.0076760075642 2312 30-31 -0.8725563542174299 2312 32-33 0.09847133985417855 2312 34-35 0.1948005907908268 2312 36-37 0.14582579452106614 2312 38-39 -0.07850798737299414 2312 40-41 -0.33147033685497007 2312 42-43 -0.3450400243593279 2312 44-45 -0.21192116833434227 2312 46-47 -0.14255325596032975 2312 48-49 -0.09067689621362263 2312 50-51 -0.3413638489047841 2312 52-53 -0.3571304618511064 2312 54-55 0.05264719578470789 2312 56-57 -0.42538123442776765 2312 58-59 -0.2290731618257098 2312 60-61 -1.262796667520746 2312 62-63 -0.8411603095686573 2312 64-65 -1.3809791604204378 2312 66-67 -1.1193243396499248 2312 68-69 -0.939318665963107 2312 70-71 -1.1797341916374755 2312 72-73 -1.1727480638826009 2312 74-75 -1.2561559505015616 2312 76-77 -1.1810068844712696 2312 78-79 -2.117156028332534 2312 80-81 -1.2261031402271811 2312 82-83 -0.9812871544844235 2312 84-85 -0.7955071461658996 2312 86-87 -0.976366676753134 2312 88-89 -0.5744792557946319 2312 90-91 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0.46706925836389956 2313 48-49 0.3857302490509582 2313 50-51 0.20497925861122468 2313 52-53 0.094588446136747 2313 54-55 0.12443688233677364 2313 56-57 0.32621970928642696 2313 58-59 0.6487495547229969 2313 60-61 0.837220184518344 2313 62-63 0.6474645640410515 2313 64-65 0.19140034750002144 2313 66-67 -0.23577192980833672 2313 68-69 0.07770189352459766 2313 70-71 -0.43967689470455085 2313 72-73 0.05753505037422357 2313 74-75 0.19674260022307877 2313 76-77 -0.2923988629006615 2313 78-79 -0.08395751131197215 2313 80-81 0.17244758441049868 2313 82-83 0.30865217817482815 2313 84-85 0.6674183678212948 2313 86-87 0.5611291102370934 2313 88-89 -0.020131429707674897 2313 90-91 0.06694389573650383 2313 92-93 0.30409736845100355 2313 94-95 0.1204405830696551 2313 96-97 0.4814973155074931 2313 98-99 0.14409809530461715 2313 100-101 0.3298412627131313 2314 1 -0.04949714782818404 2314 2 -0.0029890615583809677 2314 3 -0.29188624948063335 2314 4 -0.17187485983694017 2314 5 -0.11142214374599746 2314 6 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-0.8527366203809024 2315 24-25 -0.4322814684239944 2315 26-27 -0.2667205314492165 2315 28-29 -0.0897201237434615 2315 30-31 -0.19903604280910514 2315 32-33 -0.34827983291756226 2315 34-35 -0.15962599868917238 2315 36-37 -0.6499372556835468 2315 38-39 -0.9782983856163341 2315 40-41 -0.9725023759806319 2315 42-43 -0.991237249631304 2315 44-45 -0.9702054282405186 2315 46-47 -1.4129145694646752 2315 48-49 -1.0499542692049388 2315 50-51 -1.5590003395725134 2315 52-53 -1.911102914191666 2315 54-55 -2.144503218428291 2315 56-57 -2.083356082216966 2315 58-59 -2.050836810892477 2315 60-61 -2.6625621131740047 2315 62-63 -2.502703377739138 2315 64-65 -1.9476025759309685 2315 66-67 -2.364558394944865 2315 68-69 -1.8043501062266074 2315 70-71 -1.3814259772448203 2315 72-73 -1.183228151715717 2315 74-75 -1.4203190406303143 2315 76-77 -1.2206815026966424 2315 78-79 -1.4769723772695542 2315 80-81 -1.3891932689825452 2315 82-83 -1.208655155282905 2315 84-85 -1.724616837751885 2315 86-87 -2.314998276713208 2315 88-89 -2.020831187125104 2315 90-91 -1.506810536060474 2315 92-93 -0.5960239228346822 2315 94-95 -1.105658813583954 2315 96-97 -0.7603077476261753 2315 98-99 -0.15491602424505047 2315 100-101 -0.44043615222746624 2316 1 -4.213456064728277 2316 2 -4.399830020164046 2316 3 -4.3871087829384585 2316 4 -4.615463406366661 2316 5 -1.9967785085141259 2316 6 -2.7057995213098067 2316 7 -6.391978684946711 2316 8 -7.593414592772309 2316 9 -7.059394629996717 2316 10-11 -6.048298226285322 2316 12-13 -7.926345925463629 2316 14-15 -9.235558147174402 2316 16-17 -7.1822697862278275 2316 18-19 -7.285619602132144 2316 20-21 -5.926640388161349 2316 22-23 -5.4082907733725705 2316 24-25 -6.831069653225811 2316 26-27 -5.377967691257346 2316 28-29 -4.2647895506558555 2316 30-31 -4.477412715366533 2316 32-33 -2.564866553442677 2316 34-35 -3.1260612423145133 2316 36-37 -4.157662577571955 2316 38-39 -2.9086379463333216 2316 40-41 -2.570287026321445 2316 42-43 -2.0521877671773794 2316 44-45 -2.5555942189499348 2316 46-47 -2.4830799317469214 2316 48-49 -1.7978588387580743 2316 50-51 -1.4691682264697619 2316 52-53 -1.6199895589783395 2316 54-55 -1.8196168261031218 2316 56-57 -1.6995262412677121 2316 58-59 -1.6449418348763203 2316 60-61 -1.118747419914726 2316 62-63 -1.1288142083374666 2316 64-65 -1.9468136337446538 2316 66-67 -1.6334488266540284 2316 68-69 -2.5216374074131735 2316 70-71 -1.9790843968358338 2316 72-73 -1.5780148217485355 2316 74-75 -1.1340971269721472 2316 76-77 -1.0685923837872764 2316 78-79 -1.5769029503571588 2316 80-81 -1.2319854931683594 2316 82-83 -1.1154526811602103 2316 84-85 -1.0729695264668528 2316 86-87 -1.7197319298311093 2316 88-89 -1.8038910205005152 2316 90-91 -1.865728738534596 2316 92-93 -2.24551899983544 2316 94-95 -2.6935406616021282 2316 96-97 -3.791669777416633 2316 98-99 -5.010867804098623 2316 100-101 -5.408436657150467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1060.0 3 357.0 4 313.0 5 360.0 6 418.0 7 410.0 8 548.0 9 662.0 10 776.0 11 1031.0 12 1344.0 13 1585.0 14 1822.0 15 2148.0 16 2674.0 17 3239.0 18 2726.0 19 2109.0 20 2303.0 21 2387.0 22 2605.0 23 3046.0 24 3399.0 25 3942.0 26 4403.0 27 5351.0 28 6239.0 29 7488.0 30 9046.0 31 11339.0 32 14091.0 33 18343.0 34 24528.0 35 34428.0 36 48622.0 37 64354.0 38 51509.0 39 8218.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.895990811261356 18.34310543848443 14.434454332713686 23.326449417540523 2 15.351414824149387 19.243899644861955 40.9285141482415 24.476171382747165 3 19.6095727984053 23.180183070031735 30.23462291926818 26.975621212294794 4 12.621901607783368 15.853225307404054 37.64997765375932 33.874895431053254 5 13.619003687393812 36.60977517498647 35.30213994905838 14.469081188561345 6 32.566874181709835 35.06278452145962 17.91457408802725 14.455767208803296 7 28.709399236188503 31.907609178343964 21.130589991376322 18.252401594091204 8 26.299791437477083 33.728971855518886 20.506738173817382 19.464498533186653 9 25.713974681078415 16.500843677574792 20.936954796643626 36.84822684470316 10-11 24.73239355910836 25.71099077416767 28.179760472179243 21.376855194544724 12-13 25.787238309423365 23.083920040681562 28.404609621761768 22.724232028133304 14-15 22.59063928771958 24.880112067286206 26.660240176924223 25.869008468069993 16-17 22.097868640407736 27.95907170759448 27.52303040113222 22.420029250865564 18-19 21.872734826020313 26.642886816508373 29.73612961450857 21.74824874296274 20-21 22.410677465175954 25.736431222507335 29.637526347140764 22.215364965175954 22-23 22.141994835978593 25.59097685355574 29.829025924647677 22.43800238581799 24-25 22.082171146455593 25.842215591154588 29.85584109874627 22.21977216364355 26-27 21.766337461641672 25.919418514238586 29.93317095034145 22.381073073778296 28-29 21.614365571197837 26.320175488837215 29.869328400096222 22.196130539868726 30-31 21.743177066926314 25.880895787393683 29.716916291990035 22.659010853689967 32-33 21.48549583345265 25.99868274104407 29.727814209215087 22.788007216288193 34-35 22.413891856132746 26.078589163848182 29.29366549917951 22.21385348083956 36-37 22.16257471053694 25.770016123443142 29.658543849751272 22.40886531626864 38-39 21.84119374603543 26.04353261079681 29.5662919385486 22.548981704619166 40-41 22.017175616237513 26.124089388802346 29.526281728214055 22.332453266746082 42-43 21.83831519988317 26.129063202912807 29.18576298657137 22.84685861063266 44-45 21.92290180604037 26.02321765549787 29.284370463064512 22.769510075397246 46-47 22.237685143425754 26.366571455161107 28.865694507083596 22.530048894329546 48-49 22.192393928200193 26.354801114480512 28.745837163286286 22.706967794033005 50-51 21.87985900126853 26.569412667550534 29.023718369065037 22.527009962115898 52-53 22.202336123432538 26.381185014217767 28.710149451191402 22.706329411158297 54-55 21.70773614352601 26.390945146833523 28.892341174954538 23.00897753468593 56-57 22.03858011653366 26.40007217125207 28.528408122702587 23.032939589511685 58-59 22.308119671581867 26.25927506307472 28.480851344114505 22.951753921228903 60-61 21.90787118576113 26.210175122069963 29.213310995604047 22.66864269656486 62-63 21.72975511554085 26.169834094823823 29.3199849372361 22.780425852399233 64-65 21.404456738368495 26.36459693491856 29.006883833871672 23.22406249284127 66-67 21.47093271326176 26.397666713439307 28.79380997517232 23.33759059812661 68-69 21.652239286865 26.04074034296352 29.043067339705726 23.263953030465757 70-71 21.67682075586515 25.907890121061563 28.94710089418031 23.468188228892977 72-73 21.521687773379057 25.90763635842992 29.00928504442074 23.561390823770285 74-75 21.408107074898307 26.384718803826313 28.828209676888207 23.378964444387172 76-77 21.498341952887408 26.09748973385337 28.768549223094674 23.635619090164546 78-79 21.49227744774194 26.176065886894357 28.65756311845924 23.674093546904466 80-81 21.67997388196481 26.076366614736067 28.59982129765396 23.643838205645164 82-83 21.712392415267402 25.787403547808356 28.960356699498714 23.539847337425527 84-85 21.536888591042263 25.935997342291646 28.692796980293434 23.834317086372657 86-87 21.64369832122275 25.858610444929663 28.678956222930164 23.818735010917422 88-89 21.37611591961799 25.66711768790762 28.61164207527043 24.345124317203954 90-91 21.197490933197027 25.690014847650506 28.957500211188858 24.15499400796361 92-93 21.277945480894257 25.75466402414925 29.101964131262854 23.865426363693643 94-95 20.993864415759216 25.988101119010288 28.902825805250686 24.11520865997981 96-97 20.928577565222373 25.97038861364873 28.910192158996534 24.190841662132364 98-99 20.992339628424002 26.366343056902142 28.240405918063093 24.400911396610763 100-101 21.210289523553065 25.932504186081157 28.31925081680506 24.53795547356073 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 18.0 1 14.5 2 21.0 3 25.0 4 21.5 5 29.0 6 44.0 7 93.5 8 161.5 9 189.0 10 179.0 11 177.5 12 199.5 13 225.5 14 251.0 15 285.5 16 288.0 17 299.5 18 346.0 19 394.0 20 466.5 21 567.5 22 692.5 23 831.5 24 1018.5 25 1261.5 26 1584.0 27 2028.0 28 2497.5 29 3129.5 30 3938.5 31 4934.0 32 6020.0 33 6605.0 34 7090.0 35 7697.0 36 8441.0 37 9256.0 38 9702.0 39 10071.0 40 10452.0 41 11112.0 42 11670.0 43 12024.0 44 12479.5 45 13111.5 46 13585.5 47 14158.0 48 15198.5 49 14959.0 50 13615.0 51 12761.5 52 12419.5 53 12234.0 54 11717.0 55 10727.0 56 9571.0 57 8475.0 58 7787.5 59 6695.5 60 5317.5 61 4322.5 62 3469.0 63 2579.0 64 1829.5 65 1408.0 66 1064.0 67 823.5 68 609.0 69 441.5 70 361.5 71 285.0 72 207.5 73 165.5 74 126.0 75 95.0 76 72.5 77 56.5 78 39.0 79 26.5 80 23.0 81 20.5 82 14.5 83 6.5 84 6.0 85 4.0 86 1.0 87 2.0 88 2.0 89 2.0 90 2.0 91 1.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02892126543421987 2 0.018326346413763087 3 0.01947174306462328 4 0.0492520559869883 5 0.0564107850548645 6 0.04953840514970334 7 0.052974595102283924 8 0.0481066593361281 9 0.044956818546262566 10-11 0.058415229193869836 12-13 0.04910888140563077 14-15 0.04237967608182714 16-17 0.049967928893775916 18-19 0.054119991753144116 20-21 0.011453966508601929 22-23 0.022192060110416238 24-25 0.0074450782305912535 26-27 0.014174283554394887 28-29 0.008017776556021351 30-31 0.00973587153231164 32-33 0.004008888278010676 34-35 0.011740315671316977 36-37 0.012313013996747075 38-39 0.010738093601814309 40-41 0.002290793301720386 42-43 0.0021476187203628614 44-45 0.002004444139005338 46-47 4.2952374407257234E-4 48-49 8.590474881451447E-4 50-51 2.8634916271504823E-4 52-53 0.0037225391152956267 54-55 0.0054406340915859165 56-57 0.015606029367970129 58-59 0.010022220695026687 60-61 0.01116761734588688 62-63 0.0052974595102283925 64-65 0.0 66-67 0.00486793576615582 68-69 0.017610473506975467 70-71 0.0061565069983735374 72-73 0.0018612695576478134 74-75 0.0030066662085080063 76-77 0.005154284928870868 78-79 0.00558380867294344 80-81 0.011453966508601929 82-83 0.006442856161088585 84-85 0.014603807298467459 86-87 0.0032930153712230543 88-89 0.004724761184798296 90-91 0.0030066662085080063 92-93 0.01775364808833299 94-95 0.00901999862552402 96-97 0.00973587153231164 98-99 0.025628250062996818 100-101 0.04309554898861476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 349224.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.0555690991216 #Duplication Level Percentage of deduplicated Percentage of total 1 90.17807831217328 71.29079301234013 2 6.3628865975506415 10.060432421650791 3 1.5628906446540716 3.70665628058462 4 0.6399471708863248 2.023655511511649 5 0.328307366667982 1.2977262855685652 6 0.21935821276044984 1.0404893007806135 7 0.1516422328191088 0.8391714110483299 8 0.09762892228800651 0.6174488009609818 9 0.06878702227078987 0.489419747302612 >10 0.3546455730008033 4.756109986249748 >50 0.020628210717417002 1.177591165547684 >100 0.014114038910315914 2.0596401360731504 >500 0.0010856953007935318 0.6408659403811442 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 923 0.26430027718598953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 779 0.22306599775502256 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 538 0.15405584954069595 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 477 0.136588550615078 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 423 0.12112569582846541 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 411 0.11768950587588481 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 381 0.10909903099443337 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.8634916271504823E-4 2 0.0 0.0 0.0 0.0 2.8634916271504823E-4 3 0.0 0.0 0.0 0.0 2.8634916271504823E-4 4 0.0 0.0 0.0 0.0 2.8634916271504823E-4 5 0.0 0.0 0.0 0.0 2.8634916271504823E-4 6 0.0 0.0 0.0 0.0 2.8634916271504823E-4 7 0.0 0.0 0.0 0.0 2.8634916271504823E-4 8 0.0 0.0 0.0 0.0 2.8634916271504823E-4 9 0.0 0.0 0.0 0.0 2.8634916271504823E-4 10-11 0.0 0.0 0.0 0.0 2.8634916271504823E-4 12-13 0.0 0.0 0.0 0.0 2.8634916271504823E-4 14-15 0.0 0.0 0.0 0.0 2.8634916271504823E-4 16-17 0.0 0.0 0.0 0.0 5.726983254300965E-4 18-19 0.0 0.0 0.0 0.0 8.590474881451447E-4 20-21 0.0 0.0 0.0 2.8634916271504823E-4 8.590474881451447E-4 22-23 0.0 0.0 0.0 5.726983254300965E-4 8.590474881451447E-4 24-25 0.0 0.0 0.0 0.001145396650860193 8.590474881451447E-4 26-27 0.0 0.0 0.0 0.002290793301720386 8.590474881451447E-4 28-29 0.0 0.0 0.0 0.004581586603440772 8.590474881451447E-4 30-31 0.0 0.0 0.0 0.010594919020456785 8.590474881451447E-4 32-33 0.0 0.0 0.0 0.017610473506975463 8.590474881451447E-4 34-35 0.0 0.0 0.0 0.024339678830779102 8.590474881451447E-4 36-37 0.0 0.0 0.0 0.033932375781733214 8.590474881451447E-4 38-39 0.0 0.0 0.0 0.05798570544979727 8.590474881451447E-4 40-41 0.0 0.0 0.0 0.09334982704510572 8.590474881451447E-4 42-43 0.0 0.0 0.0 0.14890156461182508 8.590474881451447E-4 44-45 0.0 0.0 0.0 0.21504822119900122 8.590474881451447E-4 46-47 0.0 0.0 0.0 0.3552161363480173 8.590474881451447E-4 48-49 0.0 0.0 0.0 0.47218976931711454 8.590474881451447E-4 50-51 0.0 0.0 0.0 0.5596694385265617 8.590474881451447E-4 52-53 0.0 1.4317458135752411E-4 0.0 0.6636141845921243 0.001145396650860193 54-55 0.0 2.8634916271504823E-4 0.0 0.7642659152864637 0.0014317458135752411 56-57 0.0 2.8634916271504823E-4 0.0 1.0010766728518086 0.0015749203949327654 58-59 0.0 2.8634916271504823E-4 0.0 1.2796944081735506 0.0017180949762902894 60-61 0.0 2.8634916271504823E-4 0.0 1.5545896043799967 0.0017180949762902894 62-63 0.0 2.8634916271504823E-4 0.0 1.850531464045999 0.0017180949762902894 64-65 0.0 2.8634916271504823E-4 0.0 2.2050317274872286 0.0017180949762902894 66-67 0.0 2.8634916271504823E-4 0.0 2.6574634045770047 0.0017180949762902894 68-69 0.0 4.2952374407257234E-4 0.0 3.1345211096602754 0.0017180949762902894 70-71 0.0 5.726983254300965E-4 0.0 3.5407073969715714 0.0017180949762902894 72-73 0.0 5.726983254300965E-4 0.0 3.9079501981536207 0.0017180949762902894 74-75 0.0 5.726983254300965E-4 0.0 4.316140929603922 0.0017180949762902894 76-77 0.0 5.726983254300965E-4 0.0 4.828849105445215 0.0017180949762902894 78-79 0.0 5.726983254300965E-4 0.0 5.275696973862049 0.0017180949762902894 80-81 0.0 5.726983254300965E-4 0.0 5.854981330034591 0.0017180949762902894 82-83 0.0 5.726983254300965E-4 0.0 6.573717728449362 0.0020044441390053374 84-85 0.0 5.726983254300965E-4 0.0 7.593120747714933 0.0020044441390053374 86-87 0.0 5.726983254300965E-4 0.0 8.62727074886033 0.0020044441390053374 88-89 0.0 5.726983254300965E-4 0.0 9.617036629784895 0.0020044441390053374 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTATAC 90 5.671627E-7 36.95375 3 GTATAGG 85 6.383406E-4 27.952217 1 CGCCGGG 35 0.008402505 27.134144 60-61 GGTATCA 1250 0.0 25.08991 1 GTATCAA 1820 0.0 24.803755 1 CCTACAC 160 1.8493265E-6 23.755981 3 CGACAGC 105 0.0021845063 22.634474 8 AGGCGAC 105 0.0021863272 22.63123 5 GGCGACA 105 0.0021863272 22.63123 6 ACATGGG 1115 0.0 22.584162 3 GTACATG 1185 0.0 22.45613 1 TACATGG 1185 0.0 21.651022 2 GAGTACT 1115 0.0 21.31803 12-13 CAACGCA 2090 0.0 21.147747 5 TGGACCC 180 5.1277802E-6 21.12248 5 TATACTG 180 5.1277802E-6 21.12248 5 ATCAACG 2120 0.0 21.066624 3 TCAACGC 2100 0.0 21.047043 4 TATCAAC 2130 0.0 20.967722 2 GCGACAG 115 0.0037035018 20.666258 7 >>END_MODULE