FastQCFastQC Report
Thu 26 May 2016
SRR936545_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936545_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349224
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11950.3421872494444826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10160.290930749318489No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9810.28090852862346233No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA6350.18183171832405562No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC6230.17839552837147504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5300.15176505623897557No Hit
CCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAA4710.13487045563878772No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT250.00501892656.9983676
ACCGTGC502.8233992E-547.498648
GTATACC550.002577538434.5444683
TAAGGTG2600.032.8836755
GGTAAGG2250.029.5547083
TACCGTG650.00583939329.2299337
AGGTAAG2300.028.9122142
CCTATTC856.399251E-427.9403783
AGCGTGC700.00838488427.1420788
AGGGACG700.00838488427.1420785
AAGGTAA2550.026.0851551
GTATAGG1309.975056E-625.5835151
GTAAGGT2600.025.576194
GGTGTGC3500.024.4278728
GTACATG11750.023.8572121
GATTGTA1050.00219354722.61849
GGCGACA1302.9364042E-421.922456
GTGTGCA3700.021.82379
ACATGGG12050.021.6798763
GTATAGA1100.002869102421.5964761