##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936545_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 349224 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.997623301949464 31.0 31.0 34.0 30.0 34.0 2 32.142198130712664 33.0 31.0 34.0 30.0 34.0 3 32.245776349849955 34.0 31.0 34.0 30.0 34.0 4 35.96212173275605 37.0 35.0 37.0 35.0 37.0 5 35.747007651249625 37.0 35.0 37.0 35.0 37.0 6 35.85765296772272 37.0 35.0 37.0 35.0 37.0 7 35.806803083407786 37.0 35.0 37.0 35.0 37.0 8 35.76903076535404 37.0 35.0 37.0 33.0 37.0 9 37.44281607220581 39.0 37.0 39.0 34.0 39.0 10-11 37.30625042952374 39.0 37.0 39.0 34.0 39.0 12-13 37.21585715758367 39.0 37.0 39.0 33.0 39.0 14-15 38.36608165532724 40.0 38.0 41.0 33.5 41.0 16-17 38.290455409708386 40.0 38.0 41.0 33.0 41.0 18-19 38.41260623553937 40.0 38.0 41.0 34.0 41.0 20-21 38.41320613703526 40.0 38.0 41.0 34.0 41.0 22-23 38.327645007216 40.0 38.0 41.0 34.0 41.0 24-25 38.18481834009117 40.0 38.0 41.0 33.0 41.0 26-27 37.96119252972304 40.0 37.0 41.0 33.0 41.0 28-29 37.89994244381829 40.0 37.0 41.0 33.0 41.0 30-31 37.905456096946374 40.0 37.0 41.0 33.0 41.0 32-33 37.77067440954802 40.0 37.0 41.0 33.0 41.0 34-35 37.600438400568116 40.0 37.0 41.0 33.0 41.0 36-37 37.38439082079124 40.0 36.5 41.0 32.0 41.0 38-39 37.188105628479136 39.0 36.0 41.0 31.0 41.0 40-41 36.95322200077887 39.0 36.0 41.0 31.0 41.0 42-43 36.73243247886744 39.0 36.0 41.0 31.0 41.0 44-45 36.480754472773924 39.0 35.0 40.5 30.0 41.0 46-47 36.13900247405677 39.0 35.0 40.0 30.0 41.0 48-49 35.77246266006918 39.0 35.0 40.0 28.5 41.0 50-51 35.45343962614253 38.5 35.0 40.0 27.5 41.0 52-53 35.17993035988363 38.0 34.0 40.0 27.0 41.0 54-55 34.928301033147775 38.0 34.0 40.0 26.5 41.0 56-57 34.57810316587634 38.0 34.0 40.0 26.0 41.0 58-59 34.19973569972281 37.5 33.0 40.0 25.0 41.0 60-61 33.844273303094866 37.0 33.0 40.0 24.0 41.0 62-63 33.44660303988272 36.5 32.5 39.0 23.0 40.5 64-65 33.039245584495916 36.0 32.0 39.0 22.0 40.0 66-67 32.54745951022839 35.5 31.5 38.5 21.5 40.0 68-69 32.23720591940989 35.0 31.0 38.0 20.5 40.0 70-71 31.795125764552264 35.0 31.0 38.0 19.5 40.0 72-73 31.342036916134056 35.0 30.5 37.0 18.0 39.0 74-75 30.811948777861772 34.0 30.0 36.5 15.5 39.0 76-77 29.3938575241106 32.5 28.5 35.0 13.0 37.0 78-79 30.163322108446152 34.0 30.0 35.5 12.0 37.5 80-81 29.962990802464894 34.0 30.0 35.0 7.5 37.0 82-83 29.626302888690354 34.0 29.0 35.0 7.0 37.0 84-85 29.197698325430096 34.0 29.0 35.0 3.5 36.0 86-87 28.843452912743683 34.0 29.0 35.0 2.0 36.0 88-89 28.418107002955125 33.0 28.5 35.0 2.0 35.0 90-91 27.928992566375737 33.0 27.0 35.0 2.0 35.0 92-93 27.466406947976083 33.0 26.5 35.0 2.0 35.0 94-95 26.87886428195084 32.5 25.5 34.0 2.0 35.0 96-97 26.122208095663527 32.0 24.5 34.0 2.0 35.0 98-99 25.18283966737681 32.0 21.0 34.0 2.0 35.0 100-101 22.120356848326573 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32897130987998935 1101 2 0.42650856505130363 1101 3 0.4382414404720123 1101 4 0.2654745232801474 1101 5 0.1861366566719198 1101 6 0.23492271550705368 1101 7 0.27316399607462927 1101 8 0.19901744427909307 1101 9 0.2046295466522139 1101 10-11 0.1405700055450012 1101 12-13 0.28538233192278284 1101 14-15 0.5651904643031074 1101 16-17 0.43950971784845905 1101 18-19 0.48587356592978637 1101 20-21 0.30054279856202726 1101 22-23 0.40143171688492174 1101 24-25 0.46815085507109444 1101 26-27 0.32733468167062085 1101 28-29 0.24588355678265827 1101 30-31 0.305252436006775 1101 32-33 0.36837675477030274 1101 34-35 0.37219988844904606 1101 36-37 0.4770933938794144 1101 38-39 0.5278993795290816 1101 40-41 0.4515520495198828 1101 42-43 0.5762842839975235 1101 44-45 0.4757635314155024 1101 46-47 0.6470043649169668 1101 48-49 0.6245652460770401 1101 50-51 0.71342940681361 1101 52-53 0.7731028828492086 1101 54-55 0.5885632770595208 1101 56-57 0.7714723934026253 1101 58-59 0.7629948604714869 1101 60-61 0.9174052716649896 1101 62-63 0.9761151834781785 1101 64-65 0.9125047033611722 1101 66-67 0.8433811345542139 1101 68-69 0.7344058088383356 1101 70-71 0.7554355275428719 1101 72-73 1.129452586810391 1101 74-75 0.9083217114380062 1101 76-77 0.65797122386358 1101 78-79 0.5310449403125048 1101 80-81 0.7787169511818774 1101 82-83 0.8787341793760852 1101 84-85 0.7949337376930359 1101 86-87 0.7637093780772339 1101 88-89 0.5259244326168862 1101 90-91 0.604125827934638 1101 92-93 0.7528217213805029 1101 94-95 0.5832745561892807 1101 96-97 0.5240092615690664 1101 98-99 0.44587396351047914 1101 100-101 0.7095238574852587 1102 1 0.2884421911937025 1102 2 0.3321000264509344 1102 3 0.28304557885885373 1102 4 0.25238318898085055 1102 5 0.11174324301117622 1102 6 0.27876438662223535 1102 7 0.26733644580644267 1102 8 0.2830450631814543 1102 9 0.3139167679847361 1102 10-11 0.3515462314277116 1102 12-13 0.2643257786296189 1102 14-15 0.49240804736317045 1102 16-17 0.5051626004840131 1102 18-19 0.23334188617178597 1102 20-21 0.1774278553758748 1102 22-23 0.200368522599355 1102 24-25 0.47432713687794603 1102 26-27 0.19062000285316572 1102 28-29 0.16846515519645777 1102 30-31 0.2839806065245867 1102 32-33 0.45927573542563493 1102 34-35 0.4299550741426401 1102 36-37 0.6285533000793677 1102 38-39 0.7118477195987296 1102 40-41 0.39310257825726325 1102 42-43 0.47262840179411114 1102 44-45 0.5922327159397653 1102 46-47 0.5498210671717345 1102 48-49 0.5754560605359771 1102 50-51 0.39483256271487477 1102 52-53 0.2853188136049454 1102 54-55 0.5328401079398191 1102 56-57 0.5109897934382133 1102 58-59 0.3442040294895108 1102 60-61 0.7114606302150293 1102 62-63 0.5004453647232552 1102 64-65 0.8073776348032524 1102 66-67 0.4873611861569529 1102 68-69 0.713527990388453 1102 70-71 0.6998887101352125 1102 72-73 0.5721144490844701 1102 74-75 0.633887466795283 1102 76-77 0.32475784146952336 1102 78-79 0.5237946606917703 1102 80-81 0.8960043440998433 1102 82-83 0.9434608183348807 1102 84-85 0.7638791544550259 1102 86-87 0.5569242856752936 1102 88-89 0.582168713101904 1102 90-91 0.8491363454871923 1102 92-93 1.0190754708403276 1102 94-95 1.4360192878804767 1102 96-97 1.6144880167484068 1102 98-99 1.333887448855812 1102 100-101 1.8550092536569949 1103 1 0.12856639201122277 1103 2 0.18928437120864672 1103 3 0.03510685824955573 1103 4 0.20675554687485231 1103 5 0.007537272288331565 1103 6 0.055205098637046035 1103 7 0.014646301516890503 1103 8 0.05608379077814618 1103 9 0.2726475089972027 1103 10-11 0.18426052587624042 1103 12-13 0.09879347069543343 1103 14-15 0.10165367151158478 1103 16-17 0.09251040644425501 1103 18-19 -0.09183476372515287 1103 20-21 0.11967163660076352 1103 22-23 0.16473721211477965 1103 24-25 0.0018891062731896113 1103 26-27 0.012313876660940082 1103 28-29 -0.013999558495335407 1103 30-31 0.028401614105902695 1103 32-33 0.15083933812276484 1103 34-35 0.0034656687693370714 1103 36-37 0.039755611157872295 1103 38-39 -0.013092669249395783 1103 40-41 -0.1656799130958433 1103 42-43 -0.0983689167958346 1103 44-45 -0.0884141851537592 1103 46-47 -0.2102767999281525 1103 48-49 -0.2280487694958211 1103 50-51 -0.2774010867977452 1103 52-53 -0.16993788555307532 1103 54-55 -0.12379201482858804 1103 56-57 -0.41244153800852246 1103 58-59 -0.06591851182341912 1103 60-61 -0.06553579748297267 1103 62-63 -0.35822161637586447 1103 64-65 -0.1526293962556835 1103 66-67 -0.2924105446189955 1103 68-69 -0.08121531045352626 1103 70-71 -0.3325840827236739 1103 72-73 -0.24760657945900633 1103 74-75 -0.2617526215951713 1103 76-77 -0.4142577666078999 1103 78-79 -0.47255988809766336 1103 80-81 -0.08464386712888938 1103 82-83 -0.1863528367676608 1103 84-85 0.0499613698063186 1103 86-87 -0.0051990504529939585 1103 88-89 -0.1845475203467224 1103 90-91 -0.061690393498935236 1103 92-93 -0.09654941998173783 1103 94-95 0.02384638497023417 1103 96-97 0.2956720732763749 1103 98-99 0.17208356736190922 1103 100-101 0.5050197347386458 1104 1 0.03644162632832604 1104 2 0.036004658347000174 1104 3 -0.20965824339684147 1104 4 -0.17037147115848228 1104 5 -0.22142141524527403 1104 6 -0.18366029799459938 1104 7 -0.05953231713838392 1104 8 -0.49247979089498983 1104 9 -0.38397440128659355 1104 10-11 -0.39068098566887954 1104 12-13 -0.23664508373152415 1104 14-15 -0.322939815562826 1104 16-17 -0.2126812613122624 1104 18-19 -0.10393569808730518 1104 20-21 -0.4660302975143509 1104 22-23 -0.1495655764403736 1104 24-25 -0.18474718999081574 1104 26-27 -0.17459488134943513 1104 28-29 -0.37805386193780066 1104 30-31 -0.39636443207454164 1104 32-33 -0.5626598699217595 1104 34-35 -0.3546017418995433 1104 36-37 -0.0760515295195816 1104 38-39 -0.03979392039374119 1104 40-41 0.04831699173871584 1104 42-43 -0.15772016721360416 1104 44-45 -0.25048169754169436 1104 46-47 -0.3838131354266494 1104 48-49 -0.182516778853973 1104 50-51 0.12772773311593966 1104 52-53 0.1905744151865676 1104 54-55 0.25981595994508666 1104 56-57 -0.34675827660508673 1104 58-59 -0.1415684239090993 1104 60-61 -0.013232224237995638 1104 62-63 0.07350128639524911 1104 64-65 -0.11896039005082315 1104 66-67 -0.04794878371689748 1104 68-69 -0.12978070853830204 1104 70-71 -0.4346845811470317 1104 72-73 0.03170303880391856 1104 74-75 -0.019950087337647915 1104 76-77 -0.3815768883365429 1104 78-79 -0.11735695858896733 1104 80-81 0.21038134466471092 1104 82-83 -0.17886195515410463 1104 84-85 -0.34414546426084414 1104 86-87 -0.6826315889245933 1104 88-89 -0.12129461772091688 1104 90-91 -0.3161515004928468 1104 92-93 -0.8634384154334462 1104 94-95 -0.7227078821440003 1104 96-97 -0.12616228451312494 1104 98-99 0.05532644339545101 1104 100-101 0.8051363483660907 1105 1 -0.19056116826878622 1105 2 -0.16901979437773207 1105 3 -0.23935083771449328 1105 4 -0.11057873711096988 1105 5 -0.333525513987702 1105 6 -0.19512979030945843 1105 7 -0.00561988118123935 1105 8 -0.11547117422055919 1105 9 -0.07683071241839912 1105 10-11 -0.42459458604149347 1105 12-13 -0.5654614554129367 1105 14-15 -0.6445950321441032 1105 16-17 -0.0026061565148651766 1105 18-19 -0.36138795705330296 1105 20-21 -0.30899431707653235 1105 22-23 -0.4498974348474505 1105 24-25 -0.47174649134154123 1105 26-27 -0.48802641372017774 1105 28-29 -0.5654374203914543 1105 30-31 -0.5945188335649974 1105 32-33 -0.5840123384825375 1105 34-35 -0.6406986212660968 1105 36-37 -0.7083727917459441 1105 38-39 -0.42784992877753325 1105 40-41 -0.07358275208988374 1105 42-43 -0.04273414019916544 1105 44-45 0.0441154147079601 1105 46-47 -0.17389813775547935 1105 48-49 0.1497491736378791 1105 50-51 0.2832354182579948 1105 52-53 -0.02816102962014355 1105 54-55 0.022551754076431507 1105 56-57 0.18431706107539014 1105 58-59 0.2659915434872744 1105 60-61 0.2813794432049548 1105 62-63 0.32967268835147223 1105 64-65 0.5168541185658455 1105 66-67 0.6885439969072351 1105 68-69 0.29345520203458975 1105 70-71 0.458742293561869 1105 72-73 0.3383518511001462 1105 74-75 0.22718253958363732 1105 76-77 0.07787234314924874 1105 78-79 0.2040508197063282 1105 80-81 0.5288780843014109 1105 82-83 0.0827385339003861 1105 84-85 -0.15818831474989992 1105 86-87 -0.3793159158924553 1105 88-89 -0.02428425442329285 1105 90-91 0.125977050974317 1105 92-93 -0.19008606331420808 1105 94-95 -0.3616080917387805 1105 96-97 -0.3414190381228117 1105 98-99 -0.38531747835583374 1105 100-101 -0.5090374988626003 1106 1 0.14964846598634196 1106 2 0.19499670874447972 1106 3 0.15538600439077044 1106 4 -0.1717963999120471 1106 5 -0.2195090108654938 1106 6 -0.17338581171890866 1106 7 -0.02368410954876765 1106 8 0.05641776422725542 1106 9 0.09015189239872257 1106 10-11 0.38935501253121174 1106 12-13 0.014517358146385106 1106 14-15 0.10264515518863959 1106 16-17 -0.027890500850283217 1106 18-19 0.15667627131423245 1106 20-21 0.021876557142917363 1106 22-23 -0.2327364178983018 1106 24-25 -0.3009726287188812 1106 26-27 -0.458755673309831 1106 28-29 0.027169626887761922 1106 30-31 0.028864306488522118 1106 32-33 0.36438752770835947 1106 34-35 0.178659095058606 1106 36-37 0.14123135633612804 1106 38-39 0.5067787509726926 1106 40-41 0.5744156868039667 1106 42-43 0.23882250565274887 1106 44-45 0.6535043531557747 1106 46-47 0.8282762601035785 1106 48-49 0.7021577374202153 1106 50-51 0.46577333083605055 1106 52-53 0.3797337072219662 1106 54-55 0.35046767735921946 1106 56-57 0.5316353037158876 1106 58-59 0.3700559948699649 1106 60-61 0.31720125408808997 1106 62-63 0.4401767026244414 1106 64-65 0.6234274905551374 1106 66-67 -0.19582264002407612 1106 68-69 -0.25800554729636715 1106 70-71 0.011318118717976944 1106 72-73 -0.054823884849898974 1106 74-75 -0.1550933301755073 1106 76-77 0.029533806128736018 1106 78-79 -0.22898887699304993 1106 80-81 -0.4513293198020669 1106 82-83 0.1912632885837091 1106 84-85 0.15157974947846853 1106 86-87 0.34344835707810617 1106 88-89 0.585132560920151 1106 90-91 0.7877745532044678 1106 92-93 0.5915263363289682 1106 94-95 0.64499873252527 1106 96-97 0.7424067870332927 1106 98-99 0.8960719061223124 1106 100-101 0.939529628702072 1107 1 0.03483537102465206 1107 2 -0.021143682866075153 1107 3 0.024107465577984044 1107 4 -0.022949561432049848 1107 5 -0.17423387736189255 1107 6 -0.06538333513041295 1107 7 -0.060774891995947655 1107 8 -0.39648835758993783 1107 9 0.11613672329416858 1107 10-11 -0.0016944418453874732 1107 12-13 -0.2237091723079132 1107 14-15 0.0697239416602713 1107 16-17 -0.07661931440960501 1107 18-19 -0.18751148505137394 1107 20-21 0.008845444999892038 1107 22-23 0.07949754232149786 1107 24-25 0.07963839209994461 1107 26-27 -0.1323877451308988 1107 28-29 -0.363067196887485 1107 30-31 -0.3318566129449536 1107 32-33 -0.2808024730655774 1107 34-35 0.011518393874489163 1107 36-37 -0.12100647355319438 1107 38-39 -0.7972998807121598 1107 40-41 -0.6561875946757922 1107 42-43 -1.230249160626954 1107 44-45 -0.906136928478773 1107 46-47 -0.8330696109590434 1107 48-49 -0.8995178294863706 1107 50-51 -0.5941678501870911 1107 52-53 -0.6396726854720995 1107 54-55 -0.12135145428693761 1107 56-57 -0.9151857627650344 1107 58-59 -0.4448577114995089 1107 60-61 -0.4192258800346522 1107 62-63 -0.31485386776484603 1107 64-65 -0.7100219328640982 1107 66-67 -0.6008996464477292 1107 68-69 -0.5110238895299375 1107 70-71 -0.552628170268946 1107 72-73 -0.08110861513393175 1107 74-75 -0.438440207352194 1107 76-77 -0.43301746699254196 1107 78-79 -0.42721269867867306 1107 80-81 -0.3091866837831816 1107 82-83 -0.7208468422111238 1107 84-85 -0.5365829314285833 1107 86-87 -0.37547819659420867 1107 88-89 -0.43511548489673757 1107 90-91 -0.18861078848735247 1107 92-93 -0.22398716881768976 1107 94-95 -0.5696034654470239 1107 96-97 -0.015032652638556243 1107 98-99 0.01940794822431613 1107 100-101 0.26642963644141204 1108 1 -0.37226369807396154 1108 2 -0.6413409633221931 1108 3 -0.5918515646700016 1108 4 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74-75 0.2715517346300764 2316 76-77 0.2515178539851668 2316 78-79 0.5069532964865147 2316 80-81 -0.3074609249865148 2316 82-83 0.2737408558818153 2316 84-85 0.09173428586929333 2316 86-87 0.03579631320971188 2316 88-89 0.27979856291416993 2316 90-91 -0.20908486853032926 2316 92-93 -0.11744674442875791 2316 94-95 -0.5124788347728355 2316 96-97 -0.02057925484108125 2316 98-99 0.23221348139648867 2316 100-101 -0.45112341217529206 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 7.0 5 11.0 6 14.0 7 56.0 8 150.0 9 258.0 10 440.0 11 657.0 12 998.0 13 1311.0 14 1661.0 15 2160.0 16 2737.0 17 3529.0 18 3162.0 19 2284.0 20 2181.0 21 2133.0 22 2327.0 23 2575.0 24 3081.0 25 3721.0 26 4246.0 27 5322.0 28 6454.0 29 7956.0 30 9956.0 31 12516.0 32 15557.0 33 20258.0 34 26903.0 35 37023.0 36 53200.0 37 69590.0 38 42454.0 39 2334.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.496132913205386 19.6078487539387 14.338012030936694 22.55800630191922 2 16.204723191862847 20.774140710683735 39.02460675862582 23.996529338827592 3 20.2618459221163 24.09646834453471 29.971106459381524 25.670579273967466 4 13.553192220465949 16.909490756649028 36.69392710695714 32.84338991592789 5 13.796016310448309 37.8424735986072 34.04720179598195 14.314308294962547 6 32.91383180995579 36.38438366206217 16.678979680663414 14.022804847318627 7 29.29609648821387 32.83021785444299 20.03212837605663 17.84155728128651 8 27.045678418436303 34.807172473827684 18.923098068861247 19.224051038874762 9 26.630433226332052 17.08021087175023 19.67057732011901 36.61877858179871 10-11 25.357077405905663 26.614150230224727 26.840652417932333 21.188119945937277 12-13 26.463661003068278 23.928285586244716 27.29666383653787 22.311389574149135 14-15 22.83010682484934 25.98431059242619 25.619637101912424 25.565945480812047 16-17 22.454139868390154 28.861672212454252 26.43278906343961 22.25139885571598 18-19 22.30817469589719 27.36452821111951 29.017049229148057 21.310247863835247 20-21 22.95689872402813 26.383925503401827 29.075178109179205 21.583997663390832 22-23 22.4378293943225 26.189327373393027 29.21463659779423 22.158206634490245 24-25 22.96460683809633 26.495475631407135 28.97586048908997 21.564057041406564 26-27 22.54105754499506 26.835383227617015 28.890336621755132 21.733222605632793 28-29 22.11278394650745 27.407773315292484 28.7374984786982 21.74194425950187 30-31 22.38374090830995 26.943330851612163 28.607754424145238 22.065173815932653 32-33 22.307888346734476 27.005303186493485 28.590102627539913 22.096705839232126 34-35 22.79563056542339 26.89816823382689 28.662494254700405 21.64370694604931 36-37 23.11268747118306 26.523753747991762 28.662435886788458 21.701122894036722 38-39 22.52854405965687 26.827979621818056 28.863506845706006 21.77996947281907 40-41 22.589212592562895 26.92255928893369 28.528128239343452 21.960099879159962 42-43 22.506027684395598 27.2117735996014 28.263826448505537 22.018372267497465 44-45 22.8651648392556 26.962880026573583 28.147809827071264 22.02414530709956 46-47 23.32585962018647 27.303679014042565 27.578001511923578 21.792459853847387 48-49 23.265539877871557 27.453414370494457 27.229488363435916 22.051557388198066 50-51 22.744082893186395 27.62724410508547 27.776290338052895 21.852382663675243 52-53 23.04914629344597 27.731968357375525 27.341804775029534 21.877080574148977 54-55 22.707077822688607 27.86585741264939 27.239301223760453 22.187763540901546 56-57 23.216389926535125 27.661795257642506 27.17470208138551 21.947112734436857 58-59 23.09603170512402 27.203637842264715 27.10169578887686 22.5986346637344 60-61 22.664969495301516 27.455803624482222 27.811751432167313 22.067475448048945 62-63 22.551213707962663 27.518445812338825 27.903756745805037 22.026583733893474 64-65 22.23275474464679 27.900699633143656 27.597706646658054 22.268838975551503 66-67 22.390523699342513 27.496506380151658 27.395277313234516 22.71769260727131 68-69 22.559666904823608 27.285340175453417 27.412484482810463 22.742508436912512 70-71 22.770264272399015 27.428319440586673 27.288580649040874 22.512835637973446 72-73 22.49965279418355 27.180273555230066 27.659632348414238 22.66044130217215 74-75 22.434406840818102 27.616710290436945 27.030509911282426 22.91837295746253 76-77 22.48770807501439 27.2585785752535 27.18885035780455 23.064862991927562 78-79 22.498417852919026 27.336351583149348 27.01734491379088 23.147885650140747 80-81 22.66181178801954 27.438559916609872 26.80167925727802 23.097949038092565 82-83 22.444093802709613 27.564210869957417 26.895541536249894 23.096153791083076 84-85 22.092463831200813 27.432015257906734 27.07476603397519 23.40075487691726 86-87 22.109632506020855 27.565198466221652 26.781413676285716 23.54375535147178 88-89 21.80545333222598 27.42932748345414 26.639622430773723 24.125596753546155 90-91 21.964026685721077 27.276684647118714 26.84509652023123 23.914192146928983 92-93 21.786690453316076 27.36719876408693 27.182928592803012 23.66318218979398 94-95 21.508343093430618 27.645177271048162 26.78538190377737 24.061097731743843 96-97 21.674613060721907 27.859198488037457 26.772045874318113 23.694142576922523 98-99 21.242289563060922 28.30023883024725 25.98324179128413 24.4742298154077 100-101 20.90466263079822 28.257856484212574 25.875281355776885 24.962199529212317 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 55.0 1 39.0 2 26.5 3 32.5 4 32.5 5 31.0 6 50.0 7 98.0 8 159.5 9 198.5 10 188.5 11 184.5 12 217.5 13 235.0 14 236.0 15 277.0 16 320.5 17 341.0 18 383.0 19 452.0 20 512.0 21 574.5 22 661.5 23 768.5 24 947.0 25 1186.0 26 1490.5 27 1975.0 28 2491.0 29 3152.5 30 4006.0 31 4840.0 32 5721.0 33 6348.5 34 6836.0 35 7432.0 36 8332.5 37 9250.0 38 9726.0 39 10070.0 40 10410.5 41 10976.5 42 11357.5 43 11737.5 44 12270.0 45 12638.0 46 12985.5 47 13757.0 48 15209.5 49 14910.0 50 13482.5 51 12875.5 52 12535.5 53 12546.0 54 12277.5 55 11128.0 56 9612.0 57 8636.5 58 8140.5 59 7167.0 60 5690.5 61 4505.0 62 3670.0 63 2807.5 64 1956.5 65 1375.5 66 1029.0 67 830.5 68 676.0 69 498.5 70 368.5 71 275.0 72 211.5 73 180.0 74 147.0 75 116.0 76 94.0 77 74.0 78 49.0 79 34.5 80 30.5 81 31.0 82 26.5 83 19.0 84 12.0 85 4.5 86 4.0 87 2.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03550729617666598 2 0.0031498407898655303 3 0.0031498407898655303 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.002004444139005338 10-11 0.0 12-13 0.0015749203949327651 14-15 0.0021476187203628614 16-17 0.0028634916271504823 18-19 0.0 20-21 0.0 22-23 0.001002222069502669 24-25 0.001145396650860193 26-27 0.0054406340915859165 28-29 0.004724761184798296 30-31 0.001145396650860193 32-33 0.0 34-35 0.0073019036492337295 36-37 0.010594919020456785 38-39 0.007731427393306302 40-41 5.726983254300965E-4 42-43 5.726983254300965E-4 44-45 0.0014317458135752411 46-47 0.0 48-49 4.2952374407257234E-4 50-51 0.001002222069502669 52-53 0.0032930153712230543 54-55 0.003865713696653151 56-57 0.0027203170457929583 58-59 0.0017180949762902894 60-61 0.004438412022083248 62-63 0.0073019036492337295 64-65 0.011740315671316977 66-67 0.004581586603440772 68-69 0.004152062859368199 70-71 2.8634916271504823E-4 72-73 0.0021476187203628614 74-75 0.0074450782305912535 76-77 0.0031498407898655303 78-79 0.004295237440725723 80-81 0.006299681579731061 82-83 0.006586030742446109 84-85 0.008017776556021351 86-87 0.006013332417016013 88-89 0.012313013996747075 90-91 0.013887934391679839 92-93 0.00243396788307791 94-95 0.0037225391152956267 96-97 0.002577142464435434 98-99 0.006299681579731061 100-101 0.006299681579731061 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 349224.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.36585399503262 #Duplication Level Percentage of deduplicated Percentage of total 1 88.53173400477647 66.72269738931048 2 7.313147692375281 11.023232424553305 3 1.8390718430636248 4.158096600321262 4 0.7613569999854717 2.2952128199600446 5 0.426383020537915 1.6067360235910748 6 0.2684993208892303 1.2141408369541886 7 0.17076497710047944 0.9008893832133877 8 0.11554708661456563 0.6966643887475793 9 0.08883562109598953 0.6025655204170549 >10 0.44123167405322766 5.872319057973826 >50 0.025904277601702046 1.3373241324334504 >100 0.015237810353119718 2.1403502376291637 >500 0.0015237810353119718 0.7949872311194036 >1k 7.618905176559859E-4 0.6347839537757318 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1195 0.3421872494444826 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1016 0.290930749318489 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 981 0.28090852862346233 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 635 0.18183171832405562 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 623 0.17839552837147504 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 530 0.15176505623897557 No Hit CCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAA 471 0.13487045563878772 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.8634916271504823E-4 0.0 22-23 0.0 0.0 0.0 5.726983254300965E-4 0.0 24-25 0.0 0.0 0.0 0.001145396650860193 0.0 26-27 0.0 0.0 0.0 0.00243396788307791 0.0 28-29 0.0 0.0 0.0 0.005440634091585916 0.0 30-31 0.0 0.0 0.0 0.012026664834032025 0.0 32-33 0.0 0.0 0.0 0.019328568483265755 0.0 34-35 0.0 0.0 0.0 0.025914599225711862 2.8634916271504823E-4 36-37 0.0 0.0 0.0 0.0362231690834536 2.8634916271504823E-4 38-39 0.0 0.0 0.0 0.06041967333287518 2.8634916271504823E-4 40-41 0.0 0.0 0.0 0.09607014409089867 2.8634916271504823E-4 42-43 0.0 0.0 0.0 0.1520514054016906 2.8634916271504823E-4 44-45 0.0 0.0 0.0 0.21705266533800655 2.8634916271504823E-4 46-47 0.0 0.0 0.0 0.3579364533938103 2.8634916271504823E-4 48-49 0.0 0.0 0.0 0.4769145305019128 2.8634916271504823E-4 50-51 0.0 0.0 0.0 0.5635351522232149 2.8634916271504823E-4 52-53 0.0 0.0 0.0 0.6676230728701349 2.8634916271504823E-4 54-55 0.0 0.0 0.0 0.768990676471262 2.8634916271504823E-4 56-57 0.0 0.0 0.0 1.0113852427095504 2.8634916271504823E-4 58-59 0.0 0.0 0.0 1.29243694591437 2.8634916271504823E-4 60-61 0.0 0.0 0.0 1.570481982910682 2.8634916271504823E-4 62-63 0.0 0.0 0.0 1.8659943188326118 2.8634916271504823E-4 64-65 0.0 0.0 0.0 2.2220695026687745 2.8634916271504823E-4 66-67 0.0 0.0 0.0 2.6756465764094104 2.8634916271504823E-4 68-69 0.0 0.0 0.0 3.1528474560740385 2.8634916271504823E-4 70-71 0.0 0.0 0.0 3.5554543788513966 2.8634916271504823E-4 72-73 0.0 0.0 0.0 3.924844798753808 2.8634916271504823E-4 74-75 0.0 0.0 0.0 4.33647172015669 2.8634916271504823E-4 76-77 0.0 0.0 0.0 4.8513275147183474 2.8634916271504823E-4 78-79 0.0 0.0 0.0 5.296457288158889 2.8634916271504823E-4 80-81 0.0 0.0 0.0 5.87531212058736 2.8634916271504823E-4 82-83 0.0 0.0 0.0 6.600205026000504 2.8634916271504823E-4 84-85 0.0 0.0 0.0 7.615312807825349 2.8634916271504823E-4 86-87 0.0 0.0 0.0 8.652183126016538 2.8634916271504823E-4 88-89 0.0 0.0 0.0 9.642951229010606 2.8634916271504823E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATACGT 25 0.005018926 56.998367 6 ACCGTGC 50 2.8233992E-5 47.49864 8 GTATACC 55 0.0025775384 34.544468 3 TAAGGTG 260 0.0 32.883675 5 GGTAAGG 225 0.0 29.554708 3 TACCGTG 65 0.005839393 29.229933 7 AGGTAAG 230 0.0 28.912214 2 CCTATTC 85 6.399251E-4 27.940378 3 AGCGTGC 70 0.008384884 27.142078 8 AGGGACG 70 0.008384884 27.142078 5 AAGGTAA 255 0.0 26.085155 1 GTATAGG 130 9.975056E-6 25.583515 1 GTAAGGT 260 0.0 25.57619 4 GGTGTGC 350 0.0 24.427872 8 GTACATG 1175 0.0 23.857212 1 GATTGTA 105 0.002193547 22.6184 9 GGCGACA 130 2.9364042E-4 21.92245 6 GTGTGCA 370 0.0 21.8237 9 ACATGGG 1205 0.0 21.679876 3 GTATAGA 110 0.0028691024 21.596476 1 >>END_MODULE