FastQCFastQC Report
Thu 26 May 2016
SRR936544_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936544_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380568
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9210.2420066847449076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7940.20863551323285195No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6320.16606756217022978No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4990.13111979987807698No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4640.12192302032751046No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4420.11614218746715436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.11220071051691155No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16200.032.870481
GTACATG12600.030.9418561
ACCGAGC650.005808640329.2621888
TGTACCG650.005812377729.2583395
ACATGGG11950.029.0441073
TACATGG13000.028.8926122
ATCAACG18550.028.4499913
GGTATCA10450.028.20841
GCGACAG1354.1779822E-728.1746987
TCAACGC18900.027.923144
TATCAAC19250.027.662432
CAACGCA19150.027.5586055
AACGCAG19850.026.5867656
AGGCGAC1501.0483363E-625.3572275
AAGGCGA1501.0483363E-625.3572274
ACGCAGA21250.024.6114277
CGCAGAG21400.024.4421318
CGACAGC1801.9238178E-723.775538
GTATAGA1000.001642578423.77241
GATACTC1201.6970004E-423.7723985