FastQCFastQC Report
Thu 26 May 2016
SRR936544_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936544_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380568
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13880.36471800046246666No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10610.2787938029471737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9830.2582981228059112No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA6690.17578987198082865No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC6440.169220743730424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5520.14504635176893485No Hit
CCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAA5390.14163040507872443No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA205.390628E-447.4935610-11
TCGCGTA405.387209E-447.493569
GTAAGGT2300.041.298754
GGTAAGG2350.040.425363
TAAGGTG2400.039.577975
AAGGTAA2500.036.114091
CGTTCGT352.0543174E-433.92397338-39
AGGTAAG2850.033.3419572
GGGTCGC856.409242E-427.9337186
GTATGGG1056.875606E-527.153451
GCGTAAC350.00839536827.13917710-11
TCGGGGG908.9230103E-426.3957182
GGTGTGC4250.025.7023968
AGGCGAC1301.0008625E-525.5734565
ATTGGTC2600.025.57009720-21
CTCGGGG950.001222262325.0097581
GTACATG13200.024.8392351
ACATGGG12800.024.1209933
AAGGTGT4150.024.029736
CGGGGGT1000.001651659523.7499033
Produced by FastQC (version 0.11.5)