##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936544_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 380568 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.988343738832484 31.0 31.0 34.0 30.0 34.0 2 32.15597212587501 33.0 31.0 34.0 30.0 34.0 3 32.23552689663871 34.0 31.0 34.0 30.0 34.0 4 35.91945985999874 37.0 35.0 37.0 35.0 37.0 5 35.7516212608522 37.0 35.0 37.0 35.0 37.0 6 35.84237245380589 37.0 35.0 37.0 35.0 37.0 7 35.78910207899771 37.0 35.0 37.0 33.0 37.0 8 35.743638456202305 37.0 35.0 37.0 33.0 37.0 9 37.40482121460553 39.0 37.0 39.0 34.0 39.0 10-11 37.30089103655588 39.0 37.0 39.0 34.0 39.0 12-13 37.205557219734715 39.0 37.0 39.0 33.0 39.0 14-15 38.37763553425406 40.0 38.0 41.0 33.5 41.0 16-17 38.274848384519984 40.0 38.0 41.0 33.0 41.0 18-19 38.385592062390955 40.0 38.0 41.0 33.5 41.0 20-21 38.38800030480755 40.0 38.0 41.0 34.0 41.0 22-23 38.305978169473 40.0 38.0 41.0 34.0 41.0 24-25 38.16627646044859 40.0 37.5 41.0 33.0 41.0 26-27 37.958410849046686 40.0 37.0 41.0 33.0 41.0 28-29 37.885895556116125 40.0 37.0 41.0 33.0 41.0 30-31 37.888729478043345 40.0 37.0 41.0 33.0 41.0 32-33 37.7565954047634 40.0 37.0 41.0 33.0 41.0 34-35 37.59042536419247 40.0 37.0 41.0 33.0 41.0 36-37 37.38501792058187 40.0 36.0 41.0 32.0 41.0 38-39 37.19949785583654 39.0 36.0 41.0 31.5 41.0 40-41 37.03207048411848 39.0 36.0 41.0 31.0 41.0 42-43 36.77904999894894 39.0 36.0 41.0 31.0 41.0 44-45 36.48719019991171 39.0 35.0 40.5 30.0 41.0 46-47 36.13735521641336 39.0 35.0 40.0 30.0 41.0 48-49 35.750990624540165 39.0 35.0 40.0 28.5 41.0 50-51 35.479248123856976 38.5 35.0 40.0 28.0 41.0 52-53 35.151865106892856 38.0 34.0 40.0 27.0 41.0 54-55 34.95534832145635 38.0 34.0 40.0 26.5 41.0 56-57 34.62556100355258 38.0 34.0 40.0 26.0 41.0 58-59 34.243267957368985 37.5 33.5 40.0 25.5 41.0 60-61 33.80617918479746 37.0 33.0 40.0 24.0 41.0 62-63 33.48474648420256 36.5 32.5 39.0 23.0 40.5 64-65 33.0561200626432 36.0 32.0 39.0 22.5 40.0 66-67 32.63924449769818 35.5 31.5 39.0 22.0 40.0 68-69 32.26439558764794 35.0 31.0 38.0 21.0 40.0 70-71 31.884961426078913 35.0 31.0 38.0 20.0 40.0 72-73 31.44366709760148 35.0 31.0 37.0 18.0 39.0 74-75 30.949903827962416 34.0 30.0 36.5 17.5 39.0 76-77 29.44616862163923 32.5 28.5 35.0 13.5 37.5 78-79 30.213378423829646 34.0 30.0 36.0 13.5 37.5 80-81 30.009498959450084 34.0 30.0 35.0 8.0 37.0 82-83 29.687733860965714 34.0 29.0 35.0 7.0 37.0 84-85 29.33625790923041 34.0 29.0 35.0 4.5 36.0 86-87 28.94346739610267 34.0 29.0 35.0 2.0 36.0 88-89 28.480318891761787 33.5 29.0 35.0 2.0 35.0 90-91 27.99706097202077 33.0 27.0 35.0 2.0 35.0 92-93 27.236459712850266 33.0 25.5 34.5 2.0 35.0 94-95 26.758382207647514 32.0 25.0 34.0 2.0 35.0 96-97 26.17567425532362 32.0 25.0 34.0 2.0 35.0 98-99 25.342112316327174 32.0 22.0 34.0 2.0 35.0 100-101 22.277502312333144 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.383393081995532 1101 2 0.35822924162500414 1101 3 0.30106239884902664 1101 4 0.36390756185882367 1101 5 0.3724387011089476 1101 6 0.3037737801687399 1101 7 0.21947425959216105 1101 8 0.2377527313647363 1101 9 0.3218669568905952 1101 10-11 0.32695230111303175 1101 12-13 0.2804975265915033 1101 14-15 0.5859915519407011 1101 16-17 0.45880821823821805 1101 18-19 0.29237059248078623 1101 20-21 0.3335455517097472 1101 22-23 0.4006422313244329 1101 24-25 0.4419202139976477 1101 26-27 0.4734421352335332 1101 28-29 0.37239440089950904 1101 30-31 0.5408850335308628 1101 32-33 0.5629342041676537 1101 34-35 0.7049555288682399 1101 36-37 0.654369018301054 1101 38-39 0.41895749766661083 1101 40-41 0.4325890338063587 1101 42-43 0.7011238402267495 1101 44-45 0.6585561384219432 1101 46-47 0.5290121350457895 1101 48-49 0.44668908405704144 1101 50-51 0.4100476739590775 1101 52-53 0.4039194221941216 1101 54-55 0.15800221947845472 1101 56-57 0.2999281166100545 1101 58-59 0.12901134459053054 1101 60-61 0.42182199462791914 1101 62-63 0.34481355960424054 1101 64-65 0.277345090888403 1101 66-67 0.056911319298926344 1101 68-69 0.13505578656867812 1101 70-71 0.35547803348825013 1101 72-73 0.4308235458624168 1101 74-75 0.40496945721523403 1101 76-77 0.34354154358180367 1101 78-79 0.5457496814942502 1101 80-81 0.5117969953774022 1101 82-83 0.5863344675566609 1101 84-85 0.42760911055849604 1101 86-87 0.3671985020863424 1101 88-89 0.3767338843209487 1101 90-91 0.3288932851128372 1101 92-93 0.1452302089590809 1101 94-95 0.05444725558691843 1101 96-97 0.027965197719087342 1101 98-99 -0.20290366595128972 1101 100-101 0.5555445150244473 1102 1 0.22646261750204033 1102 2 0.3399403876218656 1102 3 0.3289735633148112 1102 4 0.27989241733947523 1102 5 0.22511207815207257 1102 6 0.17878070601599916 1102 7 0.2812989894841138 1102 8 0.2296448728367153 1102 9 0.3836547489304891 1102 10-11 0.3320543419293571 1102 12-13 0.42549475625260413 1102 14-15 0.5415368469817992 1102 16-17 0.5273856492106077 1102 18-19 0.3915371821935949 1102 20-21 0.4971317554219965 1102 22-23 0.49205648933533297 1102 24-25 0.5305295038674416 1102 26-27 0.453945875191053 1102 28-29 0.5958360852655673 1102 30-31 0.7452621690004335 1102 32-33 0.6025754452794843 1102 34-35 0.681259896769248 1102 36-37 0.6419971464754042 1102 38-39 0.7013958576259753 1102 40-41 0.5026393616297966 1102 42-43 0.6450544894094961 1102 44-45 0.6031216569370415 1102 46-47 0.4252213879777358 1102 48-49 0.5770797018426208 1102 50-51 0.6893670940347931 1102 52-53 0.7453313715892733 1102 54-55 0.7679200327576154 1102 56-57 0.3780424392621953 1102 58-59 0.5733690876877731 1102 60-61 0.6838221793171826 1102 62-63 0.6661797984074482 1102 64-65 0.5523658684301864 1102 66-67 0.5218873096950816 1102 68-69 0.37786906572649315 1102 70-71 0.3108663426580769 1102 72-73 0.5691073209108986 1102 74-75 0.5329475715378855 1102 76-77 0.5306040608964224 1102 78-79 0.8750437067966779 1102 80-81 0.9948586815903475 1102 82-83 0.7840027656451269 1102 84-85 0.8325911033556146 1102 86-87 0.8990527822599539 1102 88-89 0.6703159325248471 1102 90-91 0.6678119294936664 1102 92-93 0.7939281496738282 1102 94-95 0.7989158122403488 1102 96-97 0.7220689930833899 1102 98-99 0.4162670792698755 1102 100-101 1.0402176150336864 1103 1 0.18278441747709095 1103 2 0.20387908050793158 1103 3 0.24118055137319772 1103 4 0.25690791989677564 1103 5 0.261339524596238 1103 6 0.21297535553572544 1103 7 0.12683193950623206 1103 8 0.07463064042310208 1103 9 0.13432667762099726 1103 10-11 0.2744102316536683 1103 12-13 0.2394484753920807 1103 14-15 0.2563102057180089 1103 16-17 0.20205025189378034 1103 18-19 0.1587776816322375 1103 20-21 0.10205611382932744 1103 22-23 0.007373344822461547 1103 24-25 0.1305972637649262 1103 26-27 0.05941599846303092 1103 28-29 -0.017132989003819432 1103 30-31 0.10816036471597101 1103 32-33 0.021330194142592518 1103 34-35 0.13775180527354536 1103 36-37 0.17434753602393016 1103 38-39 0.12444442928136112 1103 40-41 0.29188906961015704 1103 42-43 0.5197865684759435 1103 44-45 0.6948611867570662 1103 46-47 0.6991159660518775 1103 48-49 0.6100438996674953 1103 50-51 0.7288804702354739 1103 52-53 0.6484772453233774 1103 54-55 0.3157984958837545 1103 56-57 0.08986725015821406 1103 58-59 0.7423393073545839 1103 60-61 0.8209537525942636 1103 62-63 0.79357832866976 1103 64-65 0.9249999423026551 1103 66-67 0.8504845380601154 1103 68-69 0.9558935953764092 1103 70-71 0.6652435186296763 1103 72-73 0.41102404711554996 1103 74-75 0.271734584318704 1103 76-77 0.5043095349888915 1103 78-79 0.35172891529303385 1103 80-81 0.59317007092341 1103 82-83 0.5711984131348942 1103 84-85 0.8098504281381587 1103 86-87 0.7432815668912127 1103 88-89 0.6620543282880114 1103 90-91 0.4870744523165591 1103 92-93 0.4925270224213065 1103 94-95 0.26750668988695026 1103 96-97 0.32134149560666714 1103 98-99 0.26035268922235844 1103 100-101 0.5710923130910466 1104 1 0.07034739519958322 1104 2 0.12828930078776324 1104 3 0.10448816154063678 1104 4 0.11625614404425733 1104 5 0.11541835270604395 1104 6 0.0796754146146128 1104 7 0.10273986451640837 1104 8 0.10517735860948818 1104 9 0.23280310294492068 1104 10-11 0.2061392614022708 1104 12-13 0.08911198262282483 1104 14-15 0.09887140373500358 1104 16-17 0.20249976513515833 1104 18-19 0.12528435827992723 1104 20-21 0.1427471532874165 1104 22-23 0.17712055325349496 1104 24-25 0.17205064098542522 1104 26-27 0.06638070158246734 1104 28-29 -0.04214977694731914 1104 30-31 0.28531536789008527 1104 32-33 0.3035428634439512 1104 34-35 0.27131044565194884 1104 36-37 0.29427399932714593 1104 38-39 0.39115623074695804 1104 40-41 0.5741531118533914 1104 42-43 0.37560840711353194 1104 44-45 0.3081990702652533 1104 46-47 0.2298562501442447 1104 48-49 0.3531342389857741 1104 50-51 0.38753295797018694 1104 52-53 0.3946774823728134 1104 54-55 0.25781770936401216 1104 56-57 0.08077790894279957 1104 58-59 0.11379411567846631 1104 60-61 0.2566137297700024 1104 62-63 0.35040414137134945 1104 64-65 0.21039406450420728 1104 66-67 0.2121702310627498 1104 68-69 0.32550906890649856 1104 70-71 0.4399291345729033 1104 72-73 0.7807294232939164 1104 74-75 0.44805294243948524 1104 76-77 0.15617712490071156 1104 78-79 0.2791407831765049 1104 80-81 0.41960842155841505 1104 82-83 0.21710582441565762 1104 84-85 0.6743790630490096 1104 86-87 0.5571124471665705 1104 88-89 0.40295673070693994 1104 90-91 0.5444772932402451 1104 92-93 0.4136438701775944 1104 94-95 0.7115624735628323 1104 96-97 1.0386802304378868 1104 98-99 0.9173080351273839 1104 100-101 1.1270649680079892 1105 1 -0.07382240206285928 1105 2 0.09555221351838838 1105 3 0.16003908560914226 1105 4 0.22369767007293007 1105 5 0.25086850719508647 1105 6 0.19811614344857276 1105 7 0.21059561916801783 1105 8 0.21173050752858558 1105 9 0.06836997090759667 1105 10-11 -0.03716273992117891 1105 12-13 0.03315204035430952 1105 14-15 0.10729665761286356 1105 16-17 0.08987256568538271 1105 18-19 0.24366516725019238 1105 20-21 0.16658353558656813 1105 22-23 0.13866242160695208 1105 24-25 0.1309807848452067 1105 26-27 0.03269661994556827 1105 28-29 0.060309282154499044 1105 30-31 0.07379955165127683 1105 32-33 0.08133786456419756 1105 34-35 0.049635607305312135 1105 36-37 0.1224785396896877 1105 38-39 0.2585809271095556 1105 40-41 0.2910083089897384 1105 42-43 0.4240757612086625 1105 44-45 0.5540242188925646 1105 46-47 0.41613538292145336 1105 48-49 0.0393065580929175 1105 50-51 0.2695872193145803 1105 52-53 0.4918088840286856 1105 54-55 0.41845504829682056 1105 56-57 0.22201033027844375 1105 58-59 0.375258348730263 1105 60-61 0.4379143034375659 1105 62-63 0.43566619959261743 1105 64-65 0.9182013622225611 1105 66-67 0.7474734516251615 1105 68-69 0.629521615604915 1105 70-71 0.6656694054106858 1105 72-73 0.7928215123087803 1105 74-75 0.651456130176232 1105 76-77 0.24074359166082004 1105 78-79 0.5938868011107807 1105 80-81 0.8233719100407768 1105 82-83 1.0284090553989174 1105 84-85 1.0641694998387656 1105 86-87 1.209067918892213 1105 88-89 0.7706041726498007 1105 90-91 1.2622508274180149 1105 92-93 0.9835990084361654 1105 94-95 0.7455813248729939 1105 96-97 0.8590313608391433 1105 98-99 0.5564198143305035 1105 100-101 1.1396846123445172 1106 1 0.11584512062682961 1106 2 0.18226455168050393 1106 3 0.02484206060666594 1106 4 0.061440665035853215 1106 5 0.11210133275690026 1106 6 0.06411551857716091 1106 7 0.20606911166574804 1106 8 0.18919703499625484 1106 9 0.13464063167100448 1106 10-11 0.19822136548396685 1106 12-13 0.1719496963244893 1106 14-15 0.21634814854080275 1106 16-17 0.17340529904056012 1106 18-19 0.4192387915805469 1106 20-21 0.2257996267873139 1106 22-23 0.3407549787788753 1106 24-25 0.3994640238127971 1106 26-27 0.28141639560148946 1106 28-29 0.03950754165902737 1106 30-31 -0.05022408677678669 1106 32-33 -0.008142577722836108 1106 34-35 -0.45416696381190746 1106 36-37 -0.35457648709595446 1106 38-39 -0.06250581571013214 1106 40-41 -0.13883844510812082 1106 42-43 -0.08254635161872415 1106 44-45 -0.5610435644142271 1106 46-47 -0.30292205190522026 1106 48-49 -0.38746727656000957 1106 50-51 -0.07830612580495 1106 52-53 -0.22026446977807979 1106 54-55 -0.33707163447425614 1106 56-57 -0.6150830270336911 1106 58-59 -0.46160068180461167 1106 60-61 -0.5326081500209412 1106 62-63 -0.42576285083146104 1106 64-65 -0.6631500364987417 1106 66-67 -0.6031441804170186 1106 68-69 -0.549290671500632 1106 70-71 -0.08146533058114258 1106 72-73 0.16670607874709376 1106 74-75 -0.18261193508472573 1106 76-77 -0.1605324414726006 1106 78-79 0.1362142184867814 1106 80-81 0.05348842569685175 1106 82-83 0.025797169125510777 1106 84-85 0.20555999112485424 1106 86-87 0.2972186043456624 1106 88-89 0.4020976915140615 1106 90-91 0.40298977595756824 1106 92-93 0.50248231751128 1106 94-95 0.633264499829366 1106 96-97 0.8847977808594116 1106 98-99 1.163552873838075 1106 100-101 1.1973823422324195 1107 1 -0.09192579555548974 1107 2 -0.19955137061988992 1107 3 -0.05193505013056665 1107 4 -0.09310774426362656 1107 5 -0.13476793154411126 1107 6 -0.1248614239128969 1107 7 0.06832630040848642 1107 8 0.017025690548415184 1107 9 -0.15632181861960248 1107 10-11 0.03741148478649592 1107 12-13 -0.19456624891869723 1107 14-15 0.11960124581825227 1107 16-17 -0.011217291965863296 1107 18-19 -0.34474675445799363 1107 20-21 -0.24014705033106765 1107 22-23 -0.2759521564306695 1107 24-25 0.07910133644662665 1107 26-27 -0.05573516068483997 1107 28-29 0.21777067640972092 1107 30-31 -0.04942108891811614 1107 32-33 -0.22211043868948366 1107 34-35 -0.09730850174399563 1107 36-37 -0.35149832863771735 1107 38-39 -0.20712668600685902 1107 40-41 0.22764643176554245 1107 42-43 0.36847077900225855 1107 44-45 0.2415599649525504 1107 46-47 0.13430550239272776 1107 48-49 0.2690742881386683 1107 50-51 0.1426688474284603 1107 52-53 0.26715284056147226 1107 54-55 0.30730068886621353 1107 56-57 0.20525337171209657 1107 58-59 0.4036605792844128 1107 60-61 0.48951969870955025 1107 62-63 0.3587803963389362 1107 64-65 0.21063590721492886 1107 66-67 0.4843347886866809 1107 68-69 0.5118733886094873 1107 70-71 0.2636731865494788 1107 72-73 0.4569396172909883 1107 74-75 0.27486741639890866 1107 76-77 -0.11299544621411783 1107 78-79 -0.2290589919751369 1107 80-81 0.135776587214135 1107 82-83 -0.04673313448415328 1107 84-85 0.3189356411707429 1107 86-87 0.7684421245353228 1107 88-89 0.46499919044339677 1107 90-91 0.46731803001079797 1107 92-93 0.6453896477345928 1107 94-95 0.7854421535461 1107 96-97 1.4057713201680677 1107 98-99 1.3020389360895273 1107 100-101 1.207840433391798 1108 1 -0.15747474778622106 1108 2 0.05657469968204509 1108 3 0.06445324414613651 1108 4 0.018330403550706365 1108 5 -0.05917785718629176 1108 6 -0.21635111521528216 1108 7 -0.11093519125442697 1108 8 -0.08440654243941736 1108 9 -0.08811823592469636 1108 10-11 0.05760639240254051 1108 12-13 -0.00974904522334441 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-1.4562260194065537 2316 92-93 -1.436926520802313 2316 94-95 -1.438864600877725 2316 96-97 -2.250060574523342 2316 98-99 -2.847787752516428 2316 100-101 -3.492283649473979 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 5.0 5 12.0 6 30.0 7 64.0 8 151.0 9 276.0 10 462.0 11 694.0 12 984.0 13 1394.0 14 1780.0 15 2463.0 16 3000.0 17 3821.0 18 3302.0 19 2479.0 20 2324.0 21 2325.0 22 2390.0 23 2799.0 24 3213.0 25 3815.0 26 4590.0 27 5828.0 28 7196.0 29 9023.0 30 11002.0 31 13725.0 32 17104.0 33 21866.0 34 29547.0 35 40182.0 36 58031.0 37 75588.0 38 46460.0 39 2640.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.599966359875744 19.634531587551052 14.453426824845334 22.312075227727874 2 16.422177955545276 20.832435443942796 38.928104025522835 23.817282574989093 3 20.36779603560232 23.948262586818103 29.883559285956423 25.800382091623153 4 13.320878266170567 17.0048453889975 36.92506989552459 32.74920644930735 5 13.964567632621744 37.86486088775371 33.94191656418503 14.228654915439515 6 32.778636143869164 36.555096592461794 16.534233041138506 14.132034222530535 7 29.452817893254295 32.79151163523996 19.99222215215152 17.76344831935423 8 26.976519307981757 34.89205608458935 18.917775535515336 19.21364907191356 9 26.619158731159743 17.111002501524037 19.618570137268502 36.65126863004772 10-11 25.42423430241113 26.561613167686193 26.985716087532317 21.028436442370353 12-13 26.487695220377148 23.863034684093456 27.32750250615206 22.32176758937733 14-15 22.80042305241514 25.930354010786523 25.724477260932687 25.54474567586565 16-17 22.52448971011751 28.77527800550756 26.51707447814845 22.183157806226482 18-19 22.25009065635199 27.53167716878899 29.00044145701838 21.217790717840646 20-21 22.978153707090453 26.350744150848204 29.107150364718 21.56395177734334 22-23 22.466609033363376 26.259239503162384 29.14243746830387 22.13171399517036 24-25 23.04581572806962 26.555175422000797 29.013868743562256 21.385140106367327 26-27 22.574580292881862 26.816584901842795 28.86355215824975 21.74528264702559 28-29 22.173813646838713 27.491413901202712 28.74155514154253 21.593217310416048 30-31 22.36874605844019 26.81758461215051 28.73344544881228 22.080223880597014 32-33 22.334681885730877 26.91051857730569 28.70051493765956 22.05428459930387 34-35 22.802568859411707 26.84991512463278 28.67749462631189 21.67002138964363 36-37 23.010624977829046 26.691200332137306 28.59717972333278 21.700994966700872 38-39 22.51291497401239 26.892050261978213 28.843485755427444 21.751549008581954 40-41 22.54333665294153 26.823134953897735 28.7020419531909 21.931486439969834 42-43 22.51673553583277 27.153415100413213 28.281643137280664 22.04820622647335 44-45 22.922069902433 26.872837063372568 28.222693458832925 21.98239957536151 46-47 23.316262313863263 27.378884664198424 27.54994521332643 21.754907808611886 48-49 23.19439992011898 27.429276393582192 27.32325010642044 22.05307357987839 50-51 22.711871087257844 27.676824741997148 27.63425673500759 21.97704743573742 52-53 23.142937849395967 27.6527817010123 27.344557798550852 21.85972265104088 54-55 22.658307012334518 27.951718266335224 27.228175468653927 22.161799252676335 56-57 23.154967851637902 27.612637532309336 27.284780743308502 21.94761387274426 58-59 23.26955831484655 27.161376467990706 27.025879743042484 22.543185474120257 60-61 22.767521565205506 27.462980387621755 27.797825869836046 21.97167217733669 62-63 22.49922798441514 27.5769223188063 27.907542099488168 22.01630759729039 64-65 22.160181767290034 27.862223758267373 27.673386572191127 22.30420790225146 66-67 22.441214379467965 27.43469103799304 27.386078019952357 22.738016562586633 68-69 22.5823150022795 27.401853294636535 27.304498174427266 22.711333528656695 70-71 22.822202602425847 27.493903848983624 27.19592819154527 22.48796535704526 72-73 22.579801769985917 27.175826659099027 27.571682327468416 22.672689243446637 74-75 22.463153081897513 27.76742756089037 26.96177485344005 22.80764450377206 76-77 22.46012069230557 27.303053833288004 27.254177785310645 22.98264768909578 78-79 22.57879287191576 27.3614209916352 27.065906660044202 22.993879476404842 80-81 22.715983516079184 27.440918600128256 26.775464398725862 23.0676334850667 82-83 22.48269626775546 27.588200740377655 26.881596082858167 23.047506909008717 84-85 22.12490339675413 27.57569225754557 27.125271409119346 23.174132936580953 86-87 22.281193947477348 27.579056642203405 26.756460207225967 23.383289203093277 88-89 21.856889165802407 27.537997836394062 26.53848024427979 24.066632753523745 90-91 22.04702362486263 27.397847313874678 26.7978925339545 23.757236527308194 92-93 21.908227989516877 27.33918474532163 27.086044145827913 23.66654311933358 94-95 21.615074837943126 27.74642370840803 26.736098332903985 23.902403120744857 96-97 21.699424077781707 27.881024276547834 26.724973149483432 23.69457849618703 98-99 21.438750933434303 28.2508492937452 26.052940186581687 24.25745958623881 100-101 20.93692272293988 28.154488380501597 26.00598231601091 24.902606580547612 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 48.0 1 38.0 2 32.0 3 34.5 4 37.0 5 38.5 6 47.5 7 94.0 8 151.5 9 188.5 10 200.0 11 200.5 12 224.0 13 251.0 14 281.0 15 315.5 16 347.0 17 366.5 18 399.0 19 463.5 20 534.5 21 586.5 22 665.0 23 822.5 24 1033.0 25 1297.0 26 1637.0 27 2177.0 28 2778.0 29 3406.5 30 4256.5 31 5287.0 32 6347.5 33 6942.0 34 7466.0 35 8227.0 36 9222.0 37 10226.5 38 10687.5 39 10912.0 40 11386.5 41 11991.5 42 12375.0 43 12905.5 44 13397.5 45 13823.5 46 14276.5 47 15087.0 48 16508.0 49 16193.5 50 14657.0 51 13868.5 52 13532.0 53 13586.5 54 13332.0 55 12172.0 56 10573.0 57 9558.0 58 8951.0 59 7692.5 60 6053.5 61 4874.0 62 3928.0 63 2963.5 64 2074.5 65 1486.5 66 1143.0 67 891.0 68 709.5 69 510.5 70 407.0 71 317.5 72 220.0 73 175.5 74 157.0 75 143.5 76 109.5 77 74.0 78 51.5 79 36.0 80 32.5 81 37.5 82 24.0 83 12.5 84 10.5 85 7.0 86 3.5 87 1.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018393559101133043 2 0.01208719598074457 3 0.008145719030501776 4 0.0 5 0.003153181560194236 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0011824430850728383 14-15 1.3138256500809317E-4 16-17 0.0 18-19 0.002627651300161863 20-21 0.0 22-23 2.6276513001618633E-4 24-25 0.0 26-27 0.0013138256500809316 28-29 0.0028904164301780497 30-31 0.0021021210401294906 32-33 0.0095909272455908 34-35 0.002627651300161863 36-37 6.569128250404658E-4 38-39 5.255302600323727E-4 40-41 2.6276513001618633E-4 42-43 0.004072859515250888 44-45 0.0023648861701456767 46-47 2.6276513001618633E-4 48-49 5.255302600323727E-4 50-51 1.3138256500809317E-4 52-53 1.3138256500809317E-4 54-55 0.002627651300161863 56-57 0.01747388114607639 58-59 0.030480755081877614 60-61 0.024699922221521516 62-63 0.018524941666141135 64-65 0.021415358096319184 66-67 0.0030217989951861427 68-69 6.569128250404658E-4 70-71 0.0 72-73 0.0 74-75 0.0018393559101133044 76-77 0.0035473292552185153 78-79 0.011824430850728386 80-81 0.01997014988123016 82-83 0.02272918374640012 84-85 0.0383637089823632 86-87 0.017211116016060207 88-89 0.04848016648798638 90-91 0.05412961678333438 92-93 0.03862647411237939 94-95 0.0383637089823632 96-97 0.05820247629858528 98-99 0.06621681276407895 100-101 0.07370561896954027 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 380568.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.68995840345585 #Duplication Level Percentage of deduplicated Percentage of total 1 88.03256855050027 65.7514888318624 2 7.660829010191883 11.443740002144393 3 1.9025983078258508 4.263149654099948 4 0.8157486097153333 2.437129189092607 5 0.41678993709895473 1.5565011532449955 6 0.2683516026283842 1.2025902022688837 7 0.17830475253630415 0.9322302185052531 8 0.12938848878343787 0.7731216676096789 9 0.08913837669426486 0.5991967482701599 >10 0.4623184443430333 6.067021810902457 >50 0.026378351813335383 1.3699443423340185 >100 0.015123588367241009 2.070809443421561 >500 0.0017585567868884894 0.8897414163223172 >1k 7.034227147553958E-4 0.6433353199212739 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1388 0.36471800046246666 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1061 0.2787938029471737 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 983 0.2582981228059112 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 669 0.17578987198082865 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 644 0.169220743730424 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 552 0.14504635176893485 No Hit CCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAA 539 0.14163040507872443 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.3138256500809317E-4 1.3138256500809317E-4 20-21 0.0 0.0 0.0 5.255302600323727E-4 2.6276513001618633E-4 22-23 0.0 0.0 0.0 7.88295390048559E-4 2.6276513001618633E-4 24-25 0.0 0.0 0.0 0.001576590780097118 2.6276513001618633E-4 26-27 0.0 0.0 0.0 0.0021021210401294906 2.6276513001618633E-4 28-29 0.0 0.0 0.0 0.004072859515250889 2.6276513001618633E-4 30-31 0.0 0.0 0.0 0.009196779550566522 2.6276513001618633E-4 32-33 0.0 0.0 0.0 0.016554203191019738 2.6276513001618633E-4 34-35 0.0 0.0 0.0 0.023780244266464863 2.6276513001618633E-4 36-37 0.0 0.0 0.0 0.03402808433709613 2.6276513001618633E-4 38-39 0.0 0.0 0.0 0.05504929473839104 2.6276513001618633E-4 40-41 2.6276513001618633E-4 0.0 0.0 0.09170503037564903 2.6276513001618633E-4 42-43 2.6276513001618633E-4 0.0 0.0 0.14609741228899958 2.6276513001618633E-4 44-45 2.6276513001618633E-4 0.0 0.0 0.20377435832755247 2.6276513001618633E-4 46-47 2.6276513001618633E-4 0.0 0.0 0.33371171512055664 2.6276513001618633E-4 48-49 2.6276513001618633E-4 0.0 0.0 0.4591820647032856 2.6276513001618633E-4 50-51 2.6276513001618633E-4 0.0 0.0 0.5444493493935381 2.6276513001618633E-4 52-53 2.6276513001618633E-4 0.0 0.0 0.6393075613293813 2.6276513001618633E-4 54-55 2.6276513001618633E-4 1.3138256500809317E-4 0.0 0.7314067394000547 2.6276513001618633E-4 56-57 2.6276513001618633E-4 2.6276513001618633E-4 0.0 0.9523922137436673 2.6276513001618633E-4 58-59 2.6276513001618633E-4 2.6276513001618633E-4 0.0 1.2159456391499022 2.6276513001618633E-4 60-61 2.6276513001618633E-4 2.6276513001618633E-4 0.0 1.4713533455256353 2.6276513001618633E-4 62-63 2.6276513001618633E-4 2.6276513001618633E-4 0.0 1.748833322822728 5.255302600323727E-4 64-65 2.6276513001618633E-4 2.6276513001618633E-4 0.0 2.0680929557923946 5.255302600323727E-4 66-67 2.6276513001618633E-4 2.6276513001618633E-4 0.0 2.4726198734523135 5.255302600323727E-4 68-69 2.6276513001618633E-4 2.6276513001618633E-4 0.0 2.9294370519854533 5.255302600323727E-4 70-71 2.6276513001618633E-4 2.6276513001618633E-4 0.0 3.3258182506148706 5.255302600323727E-4 72-73 2.6276513001618633E-4 2.6276513001618633E-4 0.0 3.6576906098253135 5.255302600323727E-4 74-75 2.6276513001618633E-4 2.6276513001618633E-4 0.0 4.03961972630384 5.255302600323727E-4 76-77 2.6276513001618633E-4 2.6276513001618633E-4 0.0 4.531253284564125 5.255302600323727E-4 78-79 2.6276513001618633E-4 2.6276513001618633E-4 0.0 4.940641357129343 5.255302600323727E-4 80-81 2.6276513001618633E-4 2.6276513001618633E-4 0.0 5.483514115742784 5.255302600323727E-4 82-83 2.6276513001618633E-4 2.6276513001618633E-4 0.0 6.1676231317399255 5.255302600323727E-4 84-85 2.6276513001618633E-4 2.6276513001618633E-4 0.0 7.124088204998843 5.255302600323727E-4 86-87 2.6276513001618633E-4 2.6276513001618633E-4 0.0 8.125748880620545 5.255302600323727E-4 88-89 2.6276513001618633E-4 2.6276513001618633E-4 0.0 9.021777973975741 5.255302600323727E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTAA 20 5.390628E-4 47.49356 10-11 TCGCGTA 40 5.387209E-4 47.49356 9 GTAAGGT 230 0.0 41.29875 4 GGTAAGG 235 0.0 40.42536 3 TAAGGTG 240 0.0 39.57797 5 AAGGTAA 250 0.0 36.11409 1 CGTTCGT 35 2.0543174E-4 33.923973 38-39 AGGTAAG 285 0.0 33.341957 2 GGGTCGC 85 6.409242E-4 27.933718 6 GTATGGG 105 6.875606E-5 27.15345 1 GCGTAAC 35 0.008395368 27.139177 10-11 TCGGGGG 90 8.9230103E-4 26.395718 2 GGTGTGC 425 0.0 25.702396 8 AGGCGAC 130 1.0008625E-5 25.573456 5 ATTGGTC 260 0.0 25.570097 20-21 CTCGGGG 95 0.0012222623 25.009758 1 GTACATG 1320 0.0 24.839235 1 ACATGGG 1280 0.0 24.120993 3 AAGGTGT 415 0.0 24.02973 6 CGGGGGT 100 0.0016516595 23.749903 3 >>END_MODULE