FastQCFastQC Report
Thu 26 May 2016
SRR936543_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936543_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220691
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.32715425640375007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.2841076437190461No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.17445206193274762No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9900.038.8705941
GGTATCA6000.035.631381
TGCTAGC550.00257286634.551642
ATCCGGA300.003958033431.65080644-45
CAACGCA12500.031.1655795
TCAACGC12700.030.674784
CTAGGAC1252.1372034E-730.4054433
TATCAAC12850.030.3167112
ATCAACG12900.030.1992033
AACGCAG13050.029.8520856
TAGGACT804.4773048E-429.6928144
TCTATAC650.00582886929.2363
TACATGG8950.028.1335262
GCTACAC1205.3483964E-627.7132933
ACATGGG8750.027.1477173
GTACATG9600.026.7235341
ACGCAGA15000.026.288047
CGCAGAG15300.025.7725878
CATGGGG3550.025.4270864
TACCTGG1951.5521437E-824.3633352
Produced by FastQC (version 0.11.5)