FastQCFastQC Report
Thu 26 May 2016
SRR936543_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936543_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220691
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8310.376544580431463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.3498103683430679No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4500.2039050074538608No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3760.17037396178367037No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC2770.125514860143821No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2520.11418680417416206No Hit
CCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAA2270.10285874820450314No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA2260.10240562596571677No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG2250.1019525037269304No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAG200.002072300571.2460868
GGCTCGA250.005017004456.996876
GGGCGTA451.5124348E-552.774889
AGCGTAG205.386007E-447.4973948-49
TGCGTAA308.285124E-539.5811660-61
AACGTTG250.001614273838.0022220-21
GATACAC651.3251368E-436.5364533
CGCGAGG352.0519513E-433.9267148-49
ACGATCG352.0519513E-433.9267182-83
TTGCGTA352.0519513E-433.9267160-61
GCTTATA1051.8865976E-631.6792871
TAGGGGG600.003945155531.664935
TTATAGG902.405103E-531.6649283
TAGTGAT1252.1416054E-730.3983337
GCGCGAG404.4906477E-429.68587148-49
CTTGCGT404.4906477E-429.68587158-59
AGCGCGA404.4906477E-429.68587146-47
AGGGCGT804.4834506E-429.6858718
GCGTAAG404.4906477E-429.68587162-63
CGCGAAT504.9405004E-528.50489428-29