FastQCFastQC Report
Thu 26 May 2016
SRR936542_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936542_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291552
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10030.34402096367028867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7690.26376083854681154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.14268466688618153No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAC851.6216924E-533.5485573
GTACATG12400.032.9623571
GTATCAA13950.031.6847481
TACATGG12950.031.1954082
GGTATCA9250.030.8284021
ACATGGG12550.029.5387693
CTACTAG700.008358167527.1583541
GTATAGG908.901739E-426.4039551
ATCAACG16950.026.3572243
GTAGACA1457.8146695E-726.2218594
CATGGGA5850.025.997744
TCAACGC17200.025.9741234
TACCTGG2207.6397555E-1125.9238832
TATCAAC17200.025.6978022
CAACGCA17450.025.5976075
AACGCAG17650.025.307556
CATGGGG6300.024.8951594
ACAGATC2301.3278623E-1024.7882488
GGAAGCG500.001652133223.7513362-63
ACGCAGA19500.022.6590027
Produced by FastQC (version 0.11.5)