FastQCFastQC Report
Thu 26 May 2016
SRR936542_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936542_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291552
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10850.3721463066622764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10290.35293875535067504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.19481944901767095No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3840.1317089232795522No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC3160.10838546811546483No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.10598452420151465No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCCG702.054137E-433.915778
GTCCTAC902.2402735E-532.000461
GTATCAA17900.028.6931511
CTACACT1004.9533337E-528.4892484
GGTAAGG1056.898118E-527.137273
TAGTACT908.951172E-426.3789334
GTGTAGC1551.2819437E-624.7745511
GGTATCA13100.024.1835541
GTTGTGC1201.7109246E-423.7410418
TCAACGC21950.023.3624824
ATCAACG22300.022.9997483
CAACGCA22300.022.9958045
TATCAAC22400.022.897072
AACGCAG22850.022.4422956
GTACATG13000.021.7849311
GTACTGG1553.590899E-521.6777321
GTACAGG2004.8431866E-721.6003131
CTTAATC1100.002875704821.5864663
GAGTACT11850.021.43705712-13
TAAGGTG1353.7962382E-421.1031465
Produced by FastQC (version 0.11.5)