##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936542_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 291552 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.649078037537045 31.0 31.0 34.0 30.0 34.0 2 31.901708786082757 33.0 31.0 34.0 30.0 34.0 3 32.16244100537811 33.0 31.0 34.0 30.0 34.0 4 35.930657309845245 37.0 35.0 37.0 35.0 37.0 5 35.77578270771595 37.0 35.0 37.0 35.0 37.0 6 35.86607534847986 37.0 35.0 37.0 35.0 37.0 7 35.81971312150148 37.0 35.0 37.0 35.0 37.0 8 35.79143343211503 37.0 35.0 37.0 33.0 37.0 9 37.469247338382175 39.0 37.0 39.0 35.0 39.0 10-11 37.347879623531995 39.0 37.0 39.0 34.0 39.0 12-13 37.23624602129294 39.0 37.0 39.0 33.5 39.0 14-15 38.42404613928218 40.0 38.0 41.0 34.0 41.0 16-17 38.33860169026451 40.0 38.0 41.0 33.0 41.0 18-19 38.38088059762924 40.0 38.0 41.0 33.5 41.0 20-21 38.4472015283723 40.0 38.0 41.0 34.0 41.0 22-23 38.37170041707826 40.0 38.0 41.0 34.0 41.0 24-25 38.27996206508616 40.0 38.0 41.0 34.0 41.0 26-27 38.0907951240259 40.0 37.5 41.0 33.0 41.0 28-29 38.0044280265613 40.0 37.0 41.0 33.0 41.0 30-31 38.015330026890574 40.0 37.0 41.0 33.0 41.0 32-33 37.83792599604873 40.0 37.0 41.0 33.0 41.0 34-35 37.6974999314016 40.0 37.0 41.0 33.0 41.0 36-37 37.470655320491716 40.0 36.5 41.0 32.5 41.0 38-39 37.30260468115465 40.0 36.0 41.0 32.0 41.0 40-41 37.08273138239491 39.0 36.0 41.0 31.5 41.0 42-43 36.85728274887499 39.0 36.0 41.0 31.0 41.0 44-45 36.56791241356602 39.0 35.5 41.0 30.5 41.0 46-47 36.16626879596093 39.0 35.0 40.5 30.0 41.0 48-49 35.589200211283064 39.0 35.0 40.0 27.5 41.0 50-51 35.49119368071562 39.0 35.0 40.0 27.5 41.0 52-53 35.24341626879596 38.0 35.0 40.0 27.0 41.0 54-55 34.998211296784106 38.0 34.0 40.0 26.5 41.0 56-57 34.73453449127428 38.0 34.0 40.0 26.0 41.0 58-59 34.34775100153661 38.0 34.0 40.0 25.0 41.0 60-61 33.99989195752387 37.0 33.0 40.0 24.0 41.0 62-63 33.70748099824388 37.0 33.0 39.5 23.5 41.0 64-65 33.294926805509824 36.0 32.5 39.0 22.5 40.0 66-67 32.91771107726923 36.0 32.0 39.0 22.0 40.0 68-69 32.45895243387115 35.0 31.5 38.5 21.0 40.0 70-71 31.984112611129405 35.0 31.0 38.0 19.5 40.0 72-73 31.564907803753705 35.0 31.0 37.0 18.0 39.0 74-75 31.174065003841513 34.5 30.5 37.0 17.5 39.0 76-77 29.768907433322358 33.5 28.5 35.5 14.0 37.5 78-79 30.55269557403139 34.0 30.0 36.0 15.5 38.0 80-81 30.383742865766656 34.0 30.0 36.0 12.0 37.0 82-83 30.053825732630884 34.0 30.0 35.0 7.5 37.0 84-85 29.612732891559652 34.0 29.5 35.0 6.5 36.5 86-87 29.23018192295028 34.0 29.0 35.0 2.0 36.0 88-89 28.84230943365163 34.0 29.0 35.0 2.0 36.0 90-91 28.412487309296456 33.5 29.0 35.0 2.0 35.0 92-93 27.962006777521676 33.0 27.5 35.0 2.0 35.0 94-95 27.464141902645153 33.0 27.0 35.0 2.0 35.0 96-97 26.81336433980902 33.0 25.5 34.5 2.0 35.0 98-99 26.005757120513664 32.0 24.5 34.0 2.0 35.0 100-101 23.304587174843597 29.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3256502948525437 1101 2 0.41350257613841634 1101 3 0.32423484118255885 1101 4 0.20506882967935525 1101 5 0.31261554013216397 1101 6 0.14973560713285394 1101 7 0.05669967837702927 1101 8 0.1796871616193556 1101 9 0.1331019157813671 1101 10-11 0.19247000621277266 1101 12-13 0.20809795008724308 1101 14-15 0.44820778754061763 1101 16-17 0.3381773219035864 1101 18-19 0.18501422408974832 1101 20-21 0.025427249201520397 1101 22-23 -0.08222532489853762 1101 24-25 0.05049241383579073 1101 26-27 -0.060164794190974646 1101 28-29 0.143629271592026 1101 30-31 -0.012303063310888263 1101 32-33 0.04726095424389598 1101 34-35 0.1692241282834317 1101 36-37 0.03350206086652463 1101 38-39 0.06430899765221909 1101 40-41 0.319623853671132 1101 42-43 0.44464796392641404 1101 44-45 0.3105675194902062 1101 46-47 0.41786878523841864 1101 48-49 0.5509316719619548 1101 50-51 0.40719015787217216 1101 52-53 0.2579038153225284 1101 54-55 0.4251067273804878 1101 56-57 0.2925332522188171 1101 58-59 0.2926391265512507 1101 60-61 0.27808624857093633 1101 62-63 0.3736410710649878 1101 64-65 0.441205422657724 1101 66-67 0.14041869327892442 1101 68-69 0.0887262528121937 1101 70-71 0.0829782922850697 1101 72-73 0.10984012781327834 1101 74-75 -0.27008183288447185 1101 76-77 0.2298938255162355 1101 78-79 0.46223000634722666 1101 80-81 0.3308075659080103 1101 82-83 0.3220849864357156 1101 84-85 0.6992351652449145 1101 86-87 0.5194863646443331 1101 88-89 0.24452351642689152 1101 90-91 0.15518058719282735 1101 92-93 0.022495278487824066 1101 94-95 -0.2163783506679735 1101 96-97 -0.20247685986351982 1101 98-99 -0.22263661683455638 1101 100-101 0.22181757122488222 1102 1 0.3896294037149559 1102 2 0.43077225473392744 1102 3 0.18725685016845972 1102 4 0.07980822936709586 1102 5 0.06566036800508357 1102 6 -0.019312004582687337 1102 7 0.02631452882247487 1102 8 -0.15884226735416718 1102 9 -0.012587901328799944 1102 10-11 0.12262912252750624 1102 12-13 0.1751291235816197 1102 14-15 0.2418338965354465 1102 16-17 0.37565757478982675 1102 18-19 0.12050236658307512 1102 20-21 0.017456036105137684 1102 22-23 0.04971157056262854 1102 24-25 -0.14563607405111156 1102 26-27 -0.1962728343842386 1102 28-29 -0.2967059597225088 1102 30-31 0.05192240330610076 1102 32-33 0.26564940499236656 1102 34-35 0.11212609945341967 1102 36-37 0.14751642968256107 1102 38-39 0.11654300056540023 1102 40-41 0.16025502223834565 1102 42-43 0.1733665032712821 1102 44-45 0.08411855095933163 1102 46-47 0.14126753682050008 1102 48-49 0.35867254055781217 1102 50-51 0.17467001042705732 1102 52-53 -0.22644784805424223 1102 54-55 0.0855241190061733 1102 56-57 0.0077036547981848 1102 58-59 0.2039595733210362 1102 60-61 0.3076029870406245 1102 62-63 0.15413631174111941 1102 64-65 -0.07838155856673978 1102 66-67 0.22392935287101778 1102 68-69 0.2613860851041494 1102 70-71 -0.07287840140909552 1102 72-73 -0.2906205611877688 1102 74-75 -0.7075038837511904 1102 76-77 -0.6087895770538907 1102 78-79 -0.24759597269389033 1102 80-81 -0.23261107906274958 1102 82-83 -0.1122560527628842 1102 84-85 0.041786384561827816 1102 86-87 0.011758429830596384 1102 88-89 -0.3636475543978861 1102 90-91 -0.1791176473459153 1102 92-93 0.006124805696497759 1102 94-95 -0.40991700265993103 1102 96-97 -0.6817781892715509 1102 98-99 -1.0549809264323464 1102 100-101 -1.0673617802999154 1103 1 0.5112730421493019 1103 2 0.4396698258947218 1103 3 0.37292345619982825 1103 4 0.25586941302087496 1103 5 0.17652015019253753 1103 6 0.0996188632666275 1103 7 0.22306157680608862 1103 8 0.2837743303728004 1103 9 0.156632038721348 1103 10-11 0.1572466060571145 1103 12-13 0.178962988132902 1103 14-15 -0.034305190296933574 1103 16-17 0.217630779259963 1103 18-19 0.1196659948939569 1103 20-21 -0.05304238605891243 1103 22-23 -0.05206082561260672 1103 24-25 0.14745782893768222 1103 26-27 -0.1329596952420431 1103 28-29 0.012664566776436459 1103 30-31 -0.0025781859108420235 1103 32-33 0.08770193625249334 1103 34-35 0.0792406764431064 1103 36-37 0.1592454057105286 1103 38-39 0.026115996239205685 1103 40-41 0.35663430394730966 1103 42-43 0.19855453860849082 1103 44-45 0.23143860252569226 1103 46-47 0.24423023029324042 1103 48-49 0.3183922472938647 1103 50-51 0.08689448230677499 1103 52-53 0.2053690755215527 1103 54-55 0.4943180828938907 1103 56-57 -0.010741998837168865 1103 58-59 -0.023515730975894655 1103 60-61 0.26375773359824706 1103 62-63 0.08542728999940863 1103 64-65 0.16105603584637862 1103 66-67 0.19141762784946081 1103 68-69 0.2372459859258882 1103 70-71 -0.11977255590256775 1103 72-73 -0.03640706693362361 1103 74-75 -0.07867100781892233 1103 76-77 -0.3201246872651389 1103 78-79 0.40321390400555046 1103 80-81 0.14749513816452975 1103 82-83 0.22527825231703602 1103 84-85 0.3110051382691488 1103 86-87 0.05423938902831438 1103 88-89 0.21081325278976948 1103 90-91 0.09136060699016824 1103 92-93 0.238331640612941 1103 94-95 0.19057414326814381 1103 96-97 -0.16268004708441097 1103 98-99 -0.27858677589334846 1103 100-101 0.7142858917000225 1104 1 0.08680067262490354 1104 2 0.29389382230435857 1104 3 0.08147183758187992 1104 4 0.04960044253510887 1104 5 -0.031614866519205975 1104 6 0.1728847984746693 1104 7 -0.06581537043676633 1104 8 0.009158464255712317 1104 9 0.04181114372778438 1104 10-11 0.1314998923062447 1104 12-13 0.13546816700169728 1104 14-15 0.23109559627697251 1104 16-17 0.12566599792987176 1104 18-19 -0.052143125111705046 1104 20-21 0.04910297658000928 1104 22-23 0.2660587194500508 1104 24-25 0.0989406270621842 1104 26-27 0.11672142344085756 1104 28-29 -0.004856190035219754 1104 30-31 0.14478631905544148 1104 32-33 -0.009462382109163059 1104 34-35 0.37764272673187804 1104 36-37 0.4140197676526256 1104 38-39 0.4125056692086986 1104 40-41 0.3144297711084434 1104 42-43 0.17409811314994528 1104 44-45 0.2515104098741361 1104 46-47 0.8614604507937642 1104 48-49 0.8366039136259005 1104 50-51 1.0253710563403828 1104 52-53 0.6723589539923438 1104 54-55 0.8111680078977344 1104 56-57 0.6964806629950999 1104 58-59 0.9896026920969447 1104 60-61 0.5017996842070787 1104 62-63 0.5732212218060582 1104 64-65 0.3661292013930222 1104 66-67 0.6202332790169223 1104 68-69 0.31588861445277416 1104 70-71 0.5172371640723945 1104 72-73 0.622637942573764 1104 74-75 0.19534843213099862 1104 76-77 0.5040949392894092 1104 78-79 0.7004910118701062 1104 80-81 0.8927709705038183 1104 82-83 0.8427210603622974 1104 84-85 0.7687563163584592 1104 86-87 0.9108107062443622 1104 88-89 0.6844317290094928 1104 90-91 0.6612782883686812 1104 92-93 0.7527639728164104 1104 94-95 0.9915803909798768 1104 96-97 0.8226957014009031 1104 98-99 0.4897459472349901 1104 100-101 0.16022665063755426 1105 1 0.1400320545719218 1105 2 0.21345624741405445 1105 3 0.0797736056396019 1105 4 -0.02217356330956477 1105 5 0.025366418447561045 1105 6 0.2104096900723036 1105 7 0.03722506817257454 1105 8 -0.11719171634306491 1105 9 -0.11975364338979233 1105 10-11 -0.06540449809745752 1105 12-13 0.03051829501563219 1105 14-15 0.11770197714643871 1105 16-17 0.0458706478096218 1105 18-19 -0.16000240461310256 1105 20-21 -0.1186082033032676 1105 22-23 -0.4973913637374494 1105 24-25 -0.2785021976923403 1105 26-27 -0.4516949106194659 1105 28-29 0.004803042915646927 1105 30-31 -0.10661235166858773 1105 32-33 -0.30919169945440217 1105 34-35 -0.39837168668847056 1105 36-37 -0.14537093776015553 1105 38-39 -0.21324969918695302 1105 40-41 -0.07080595555380143 1105 42-43 0.02166671051381286 1105 44-45 -0.22528760397032954 1105 46-47 0.1937535811438451 1105 48-49 0.11263987821302379 1105 50-51 -0.40788929037478994 1105 52-53 -0.49342124134743415 1105 54-55 -0.5423831933643086 1105 56-57 -0.5250308496381919 1105 58-59 0.04600209556064527 1105 60-61 0.1267016263982299 1105 62-63 0.11061249616964375 1105 64-65 -0.24028183970074934 1105 66-67 -0.2366281814625424 1105 68-69 -0.6528449527825586 1105 70-71 -0.1847576119247769 1105 72-73 0.04325637485568379 1105 74-75 0.09200196695337581 1105 76-77 0.2772732191614793 1105 78-79 0.4763933592231915 1105 80-81 -0.2094074214343209 1105 82-83 0.2720720254235438 1105 84-85 0.5727053521592573 1105 86-87 0.3469890809415652 1105 88-89 0.34279832297688984 1105 90-91 0.13396864982485468 1105 92-93 -0.20631898193476061 1105 94-95 0.3233347421604478 1105 96-97 -0.4589780484159114 1105 98-99 0.08351076366215437 1105 100-101 -0.29448572749059565 1106 1 0.5397988223813535 1106 2 0.5318861192495667 1106 3 0.141444272728954 1106 4 0.1193055363688913 1106 5 -0.21510049881161564 1106 6 -0.3972167761203522 1106 7 -0.28179354511486565 1106 8 0.13408766411977524 1106 9 -0.061321054286040066 1106 10-11 0.11811866661428638 1106 12-13 0.029640843813233175 1106 14-15 -0.25224277173268916 1106 16-17 -0.025978453227466503 1106 18-19 -0.14150233668139833 1106 20-21 -0.2309446011113394 1106 22-23 -0.12052890279817774 1106 24-25 -0.41535628038787564 1106 26-27 -0.2101297641134252 1106 28-29 0.18124432727777418 1106 30-31 -0.19928818156759576 1106 32-33 0.0072511699808543995 1106 34-35 -0.5138442649231507 1106 36-37 -0.18720554669997824 1106 38-39 -0.40370189790983346 1106 40-41 -0.2519854764088336 1106 42-43 -0.3121949805327944 1106 44-45 -0.4606256774271529 1106 46-47 -0.014417470891842754 1106 48-49 -0.3654974344086881 1106 50-51 -0.5757598578309668 1106 52-53 -0.4903869584798102 1106 54-55 -1.0597397442451566 1106 56-57 -1.003156840308904 1106 58-59 -0.6458710080125627 1106 60-61 -0.20934520119110545 1106 62-63 -0.4874072946007004 1106 64-65 -0.4275072823890227 1106 66-67 -0.7251533841254698 1106 68-69 -0.599750663574639 1106 70-71 -0.6444514663697767 1106 72-73 -0.4716772785114607 1106 74-75 -0.41324009635897596 1106 76-77 -0.1976996081561211 1106 78-79 -0.06960206670860458 1106 80-81 -0.33548866775468866 1106 82-83 -0.3792232057706961 1106 84-85 -0.3387513300562226 1106 86-87 -0.34231393975495905 1106 88-89 -0.4848419559959858 1106 90-91 -0.4868875161096611 1106 92-93 -0.00870564920526462 1106 94-95 -0.2628988976203601 1106 96-97 -0.38791582297322336 1106 98-99 -1.0222192757382338 1106 100-101 -0.37708502100085184 1107 1 0.6181722889874415 1107 2 0.4635081092541107 1107 3 0.2512807107116899 1107 4 0.16769263314308347 1107 5 -0.21260163465895943 1107 6 0.08949380997687939 1107 7 0.16732049395828597 1107 8 -0.11511724274073742 1107 9 -0.2767912950856726 1107 10-11 -0.13886679453973017 1107 12-13 0.3957812345447209 1107 14-15 0.34424745998016704 1107 16-17 0.13076847998470953 1107 18-19 0.013086486580753842 1107 20-21 -0.04108771787644372 1107 22-23 0.4464292980196305 1107 24-25 0.29869415123411613 1107 26-27 0.11716464751766154 1107 28-29 0.2652402382273422 1107 30-31 0.3897054576872918 1107 32-33 0.437566935541966 1107 34-35 0.794424703727465 1107 36-37 -0.3525849197122426 1107 38-39 -0.13007627319379367 1107 40-41 0.11375736775291045 1107 42-43 0.6322620844376701 1107 44-45 0.6189972212552703 1107 46-47 0.6744739421022459 1107 48-49 0.5366493170679121 1107 50-51 0.9486608600245532 1107 52-53 0.3910305790031501 1107 54-55 0.6612393561637546 1107 56-57 1.2713206699137203 1107 58-59 1.351857297303205 1107 60-61 0.935759296764374 1107 62-63 0.7606617649177068 1107 64-65 0.34344910107303406 1107 66-67 0.2409415727123303 1107 68-69 0.7114715081654808 1107 70-71 0.8611141856065991 1107 72-73 0.5893745161273394 1107 74-75 0.7379411848768278 1107 76-77 0.9079909870278833 1107 78-79 1.7237873017602716 1107 80-81 1.5625690332902948 1107 82-83 1.3059767033615195 1107 84-85 1.3852377658092898 1107 86-87 1.505141762198697 1107 88-89 1.3819799431407738 1107 90-91 1.4797185720320911 1107 92-93 1.1765510522940517 1107 94-95 0.7951996939289359 1107 96-97 1.0017366077400887 1107 98-99 1.1847321099955224 1107 100-101 0.6709613215707044 1108 1 0.3245348458843331 1108 2 0.234188892946964 1108 3 0.15796980629847468 1108 4 -0.1383631752875516 1108 5 -0.06650030871397661 1108 6 -0.16149570120460055 1108 7 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0.016615247392422816 2316 72-73 0.21606831732058396 2316 74-75 -0.04727471157377394 2316 76-77 -0.7138868570412846 2316 78-79 -0.07544669561054107 2316 80-81 -0.00408330769463916 2316 82-83 0.14485070477723028 2316 84-85 0.050509785224804205 2316 86-87 0.08768296960086275 2316 88-89 0.10088478690657254 2316 90-91 0.1121510727905104 2316 92-93 0.22152526606693357 2316 94-95 -0.15385986719959277 2316 96-97 0.25396130184465093 2316 98-99 -0.27081192883550287 2316 100-101 -1.4412214067132467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 3.0 5 4.0 6 13.0 7 48.0 8 85.0 9 185.0 10 316.0 11 537.0 12 746.0 13 1077.0 14 1385.0 15 1912.0 16 2449.0 17 3217.0 18 2854.0 19 1839.0 20 1635.0 21 1590.0 22 1671.0 23 1969.0 24 2273.0 25 2766.0 26 3235.0 27 4054.0 28 4879.0 29 6139.0 30 7682.0 31 9595.0 32 12429.0 33 15880.0 34 21338.0 35 29615.0 36 43254.0 37 60553.0 38 41535.0 39 2788.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74178695135102 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21.649482768082535 27.449477280210733 30.095660465371527 20.805379486335198 20-21 22.537660520250245 26.655279332674787 30.146594775546042 20.66046537152892 22-23 21.99967415258234 26.608185630128883 29.986708855332324 21.405431361956456 24-25 22.22090741960268 26.818886510811108 29.977842717594115 20.9823633519921 26-27 22.07565031280869 27.002730216222147 29.898611568433765 21.023007902535397 28-29 21.613983097354847 27.28021073427725 30.17935051037208 20.92645565799583 30-31 21.83727088135221 26.975290857205575 29.962579574141145 21.224858687301065 32-33 21.83414426666987 27.162122067048212 29.82388140078515 21.179852265496773 34-35 22.15558592966203 27.015824071041113 29.810375253601872 21.018214745694983 36-37 22.27386307703653 26.862200666452864 29.681848506312093 21.182087750198512 38-39 22.01528372297223 27.05486499835364 29.91284573592361 21.01700554275052 40-41 22.072975174720234 27.24366505166574 29.546627792308023 21.136731981306006 42-43 22.001478485632788 27.25181766258625 29.36577768858839 21.38092616319257 44-45 22.27517737307771 27.265633440977684 29.131545053704983 21.327644132239627 46-47 22.55932964956148 27.739076450270794 28.31170746598342 21.389886434184305 48-49 22.706511317580024 27.908636075954796 28.063344830720837 21.32150777574434 50-51 22.480573624718957 28.022514187127744 28.085796751495028 21.411115436658267 52-53 22.593806434352217 28.12640951895851 27.900371984379742 21.379412062309527 54-55 22.13059362296966 28.055674665111574 28.171449153559852 21.642282558358918 56-57 22.422196839509223 28.176183468993642 27.8944010537256 21.507218637771537 58-59 22.565962664745882 27.86166919627162 27.953249470497948 21.619118668484553 60-61 22.318209727267426 27.921044417231787 28.391663322253898 21.369082533246893 62-63 22.281353170689876 27.931398190627277 28.420357586931356 21.36689105175149 64-65 21.931777637501373 28.03991931057196 28.323464430782742 21.704838621143924 66-67 21.84514805706191 28.108718096472906 28.15690995839379 21.8892238880714 68-69 22.076164800790256 28.076123641751728 27.974769509384263 21.872942048073757 70-71 22.112386682078704 28.23558465701928 27.8773233818218 21.774705279080216 72-73 22.000192075513116 27.85729475359456 28.098589616946544 22.04392355394578 74-75 22.18283530896718 28.022788387663265 27.73501810997695 22.059358193392605 76-77 21.884995258013785 27.77363318464248 28.062954368887084 22.27841718845665 78-79 21.98905375528917 27.793033303546466 27.81498753925606 22.402925401908302 80-81 22.00771869639794 28.277530017152657 27.641166380789024 22.073584905660375 82-83 21.96728790825795 28.361342375811716 27.555031851066676 22.11633786486366 84-85 21.699190812514793 28.40165747941014 27.515118668253713 22.384033039821354 86-87 21.815478079210195 28.000397920522236 27.33868353483777 22.845440465429796 88-89 21.736370701146132 28.043272026720782 27.479555408402913 22.74080186373017 90-91 21.71273495168448 27.60014341123171 27.96587636099146 22.721245276092354 92-93 21.68287065223543 27.4152913716135 27.893779144450143 23.008058831700932 94-95 21.277124882513153 27.89943812130817 27.826373309733054 22.99706368644562 96-97 21.355113577892272 28.008905110490605 28.02537064606645 22.61061066555067 98-99 21.222797003210843 28.62232086500727 27.241417162929828 22.913464968852054 100-101 20.718565133794037 29.49489128216211 26.582999298483934 23.203544285559918 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 61.0 1 42.5 2 31.0 3 40.0 4 45.5 5 42.5 6 54.5 7 112.5 8 179.0 9 224.5 10 237.5 11 239.5 12 265.0 13 304.0 14 354.5 15 374.5 16 393.0 17 439.0 18 464.5 19 496.0 20 540.5 21 607.5 22 721.0 23 846.0 24 944.5 25 1150.5 26 1485.5 27 1904.5 28 2380.0 29 2943.5 30 3671.5 31 4436.5 32 5272.0 33 5948.5 34 6496.0 35 7191.5 36 8028.5 37 8656.5 38 9006.5 39 9297.5 40 9437.5 41 9870.0 42 10346.5 43 10596.0 44 10924.0 45 11009.0 46 11027.0 47 11327.0 48 12111.5 49 11939.0 50 10999.5 51 10348.0 52 9604.5 53 9145.0 54 8691.0 55 7922.0 56 6979.0 57 6244.5 58 5589.5 59 4742.5 60 3876.0 61 3151.5 62 2504.0 63 1910.0 64 1361.5 65 950.5 66 731.0 67 617.0 68 459.5 69 290.0 70 207.0 71 152.5 72 127.5 73 109.5 74 81.5 75 62.5 76 53.5 77 41.5 78 27.0 79 25.0 80 17.0 81 13.5 82 11.0 83 7.0 84 5.0 85 3.0 86 2.5 87 1.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0766518494127977 2 0.010632751618922182 3 0.004115903852486006 4 0.015434639446822523 5 0.0 6 0.0 7 0.0 8 0.0 9 0.005830863791021841 10-11 1.7149599385358357E-4 12-13 5.144879815607508E-4 14-15 1.7149599385358357E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0015434639446822525 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.005316375809461091 34-35 8.574799692679178E-4 36-37 0.0029154318955109206 38-39 0.0 40-41 0.004973383821753923 42-43 0.012862199539018768 44-45 0.0018864559323894196 46-47 0.0010289759631215015 48-49 0.012519207551311601 50-51 8.574799692679178E-4 52-53 0.0025724399078037538 54-55 0.012690703545165183 56-57 0.0051448798156075075 58-59 0.0015434639446822525 60-61 0.0072028317418505105 62-63 0.004973383821753923 64-65 0.021951487213258698 66-67 0.003086927889364505 68-69 0.0 70-71 0.0017149599385358357 72-73 0.0 74-75 0.0 76-77 0.0025724399078037538 78-79 0.012519207551311601 80-81 0.01783558336077269 82-83 0.013376687520579519 84-85 0.008574799692679179 86-87 0.012519207551311601 88-89 0.03206975085062013 90-91 0.02829683898584129 92-93 0.0029154318955109206 94-95 0.010289759631215015 96-97 0.011661727582043683 98-99 0.013719679508286686 100-101 0.01389117550214027 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 291552.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.4317791816284 #Duplication Level Percentage of deduplicated Percentage of total 1 89.03627553256358 68.05200950659524 2 7.062959164576102 10.796690704714786 3 1.7217157686941806 3.947813983390837 4 0.7226907607233468 2.2094616256083954 5 0.3565221989363306 1.3624812991225106 6 0.24675213275437216 1.1315822713966817 7 0.14446273018915678 0.7729080445654954 8 0.11876438708536972 0.7261898726680335 9 0.088004745587059 0.6053723353480932 >10 0.45570315643157144 6.330124407667751 >50 0.03438584418509076 1.7847357657696898 >100 0.010406242319170976 1.3531623816035974 >500 4.52445318224825E-4 0.1964212197165457 >1k 9.0489063644965E-4 0.7310465818323549 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1085 0.3721463066622764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1029 0.35293875535067504 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 568 0.19481944901767095 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 384 0.1317089232795522 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 316 0.10838546811546483 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 309 0.10598452420151465 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.4299198770716715E-4 0.0 20-21 0.0 0.0 0.0 3.4299198770716715E-4 0.0 22-23 0.0 0.0 0.0 3.4299198770716715E-4 0.0 24-25 0.0 0.0 0.0 5.144879815607508E-4 0.0 26-27 0.0 0.0 0.0 8.574799692679178E-4 0.0 28-29 0.0 0.0 0.0 0.0025724399078037538 0.0 30-31 0.0 0.0 0.0 0.008060311711118429 0.0 32-33 0.0 0.0 0.0 0.013205191526725935 0.0 34-35 0.0 0.0 0.0 0.02023652727472286 0.0 36-37 0.0 0.0 0.0 0.028811326967402042 0.0 38-39 0.0 0.0 0.0 0.039787070574031386 0.0 40-41 0.0 0.0 0.0 0.05127730216222149 0.0 42-43 0.0 0.0 0.0 0.07905965316650204 0.0 44-45 0.0 0.0 0.0 0.11164389199868291 0.0 46-47 0.0 0.0 0.0 0.13736829107672044 0.0 48-49 0.0 0.0 0.0 0.17629788168148391 0.0 50-51 0.0 0.0 0.0 0.21900038415102624 0.0 52-53 0.0 0.0 0.0 0.29840302930523543 0.0 54-55 0.0 0.0 0.0 0.3532817473383822 0.0 56-57 0.0 0.0 0.0 0.4743579189990122 0.0 58-59 0.0 0.0 0.0 0.6374506091537702 0.0 60-61 0.0 0.0 0.0 0.7876810997695094 0.0 62-63 0.0 0.0 0.0 1.0164567555701898 0.0 64-65 0.0 0.0 0.0 1.141991823071013 0.0 66-67 0.0 0.0 0.0 1.4227307650093293 0.0 68-69 0.0 0.0 0.0 1.7307375699703655 0.0 70-71 0.0 0.0 0.0 1.954539841949292 0.0 72-73 0.0 0.0 0.0 2.385680770497201 0.0 74-75 0.0 0.0 0.0 2.788696356053123 0.0 76-77 0.0 0.0 0.0 3.037365547140819 0.0 78-79 0.0 0.0 0.0 3.3066142574909447 0.0 80-81 0.0 0.0 0.0 3.8204162550762817 0.0 82-83 0.0 0.0 0.0 4.158777850949402 0.0 84-85 0.0 0.0 0.0 4.688528975963122 0.0 86-87 0.0 0.0 0.0 5.678232356492153 0.0 88-89 0.0 0.0 0.0 7.012985676654594 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTCCG 70 2.054137E-4 33.91577 8 GTCCTAC 90 2.2402735E-5 32.00046 1 GTATCAA 1790 0.0 28.693151 1 CTACACT 100 4.9533337E-5 28.489248 4 GGTAAGG 105 6.898118E-5 27.13727 3 TAGTACT 90 8.951172E-4 26.378933 4 GTGTAGC 155 1.2819437E-6 24.774551 1 GGTATCA 1310 0.0 24.183554 1 GTTGTGC 120 1.7109246E-4 23.741041 8 TCAACGC 2195 0.0 23.362482 4 ATCAACG 2230 0.0 22.999748 3 CAACGCA 2230 0.0 22.995804 5 TATCAAC 2240 0.0 22.89707 2 AACGCAG 2285 0.0 22.442295 6 GTACATG 1300 0.0 21.784931 1 GTACTGG 155 3.590899E-5 21.677732 1 GTACAGG 200 4.8431866E-7 21.600313 1 CTTAATC 110 0.0028757048 21.586466 3 GAGTACT 1185 0.0 21.437057 12-13 TAAGGTG 135 3.7962382E-4 21.103146 5 >>END_MODULE