FastQCFastQC Report
Thu 26 May 2016
SRR936541_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936541_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324497
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9960.3069365818482143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8240.25393146932020944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5260.16209703017285212No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10950.031.673041
TACATGG15050.030.6207792
GTACATG16050.029.896981
GTATCAA15700.029.0504321
ACCGTCT1004.930338E-528.510138
ACATGGG15650.027.6253683
TATCAAC18800.024.007492
CATGGGA9750.023.87664
GTGTAAG1401.7716151E-523.7547781
ATCAACG19200.023.5073323
TCAACGC19650.022.9689984
TAGATAG1252.245645E-422.8081055
CAACGCA20150.022.4025025
AACGCAG20200.022.347056
GTGTAAC1302.931219E-421.9274881
GAGTACT11750.021.63868512-13
GTAGACC2205.595939E-821.5952553
GTCCTAG2005.351176E-721.3793011
CTTAGGC1353.7826988E-421.115363
CATGGGG6200.020.6896464
Produced by FastQC (version 0.11.5)