FastQCFastQC Report
Thu 26 May 2016
SRR936541_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936541_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324497
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12330.3799726962036629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11740.3617907099295217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6100.18798324791908708No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4210.12973925799005848No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC3560.10970825616261476No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.10662656357377726No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG200.002073118271.246642
GTAAGGT1051.022272E-940.712374
GGGTTGC608.27989E-539.581476
GGGACCG500.0016144837.998215
GTGTAGG1551.8189894E-1236.7838021
AAGGCGT550.00257750434.5438276
GTAACCG300.003950605631.66517666-67
GAGGCGC753.0744646E-431.6651766
GGTAAGG1351.19216566E-831.6651743
GGTATCA14850.029.1153491
GCTTATA1852.8740033E-1028.2506221
GTATCAA20450.027.183141
ATCGCAA350.00838139427.14785428-29
GCAAACG700.00838477627.1415799
AAGGTGT2500.026.598756
TAAGGTG1806.664777E-926.3876485
GGTGTGC2701.8189894E-1224.628478
GTACATG15000.024.0729541
AGTCTAC1201.7041099E-423.7562051
TCAACGC23550.023.1942394
Produced by FastQC (version 0.11.5)