FastQCFastQC Report
Thu 26 May 2016
SRR936540_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936540_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361353
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10900.3016440987068047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8460.23412009862931815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4920.1361549509759155No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAC500.00160665138.036983
ATACGTA500.00160665138.036987
GTACTAG1001.2888977E-633.2731321
GGTATCA10850.031.980761
CTACACG600.003928640431.6974834
TGTACCG600.003928640431.6974835
GTACATG14250.030.3544371
GTATCAA16200.029.63481
TACATGG14400.028.7218652
ACATGGG14650.028.2356413
ACTATAC856.3623075E-427.9683673
ATAGACG700.00833311127.1768048
GTATAGG1056.860371E-527.1617411
AATAGAC1309.921083E-625.6018147
GTCCTAG2251.0004442E-1025.3509581
CCAATAC1151.2709513E-424.8067263
TATAAGG1151.2721482E-424.8032882
ATCAACG19450.024.445363
TCAACGC19550.024.3203184
CAACGCA19500.024.1388515
Produced by FastQC (version 0.11.5)