##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936540_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 361353 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.47404892169153 31.0 31.0 34.0 30.0 34.0 2 31.560294227528207 33.0 31.0 34.0 30.0 34.0 3 31.493046965155955 33.0 31.0 34.0 28.0 34.0 4 35.159079902477636 37.0 35.0 37.0 33.0 37.0 5 35.19366104612387 37.0 35.0 37.0 33.0 37.0 6 35.24018618912808 37.0 35.0 37.0 33.0 37.0 7 35.104772895202196 37.0 35.0 37.0 32.0 37.0 8 35.09338513863175 37.0 35.0 37.0 32.0 37.0 9 36.61263916447352 39.0 37.0 39.0 32.0 39.0 10-11 36.502016034182645 39.0 37.0 39.0 32.0 39.0 12-13 36.414164265967074 39.0 37.0 39.0 32.0 39.0 14-15 37.45952157585519 40.0 37.0 41.0 32.0 41.0 16-17 37.37651271748125 40.0 37.0 41.0 32.0 41.0 18-19 37.2696338483422 40.0 37.0 41.0 31.5 41.0 20-21 37.29248269697498 40.0 37.0 41.0 31.5 41.0 22-23 37.25114223487836 39.5 37.0 41.0 31.5 41.0 24-25 37.36723785329028 40.0 37.0 41.0 32.0 41.0 26-27 37.28397439622751 40.0 37.0 41.0 31.0 41.0 28-29 37.17441255503621 39.5 36.5 41.0 31.0 41.0 30-31 36.98966938146356 39.0 36.0 41.0 30.5 41.0 32-33 36.84266216137682 39.0 36.0 41.0 30.0 41.0 34-35 36.670077458883696 39.0 36.0 41.0 30.0 41.0 36-37 36.46835504340631 39.0 35.0 40.5 30.0 41.0 38-39 36.2792062055663 39.0 35.0 40.0 30.0 41.0 40-41 36.08809391370765 39.0 35.0 40.0 29.5 41.0 42-43 35.82085523020426 38.0 35.0 40.0 28.5 41.0 44-45 35.680398391600455 38.5 35.0 40.0 28.0 41.0 46-47 35.5483903551375 39.0 35.0 40.0 27.0 41.0 48-49 35.34471970621525 39.0 35.0 40.0 26.5 41.0 50-51 34.385436124786565 38.0 34.0 39.5 25.0 40.5 52-53 34.49671650712738 38.0 33.5 39.5 25.0 40.5 54-55 34.94018452870185 38.0 34.0 40.0 26.0 41.0 56-57 34.80684123281113 38.0 34.0 40.0 25.0 41.0 58-59 34.61531383439463 38.0 34.0 40.0 24.5 41.0 60-61 34.33797422465013 38.0 34.0 40.0 23.5 41.0 62-63 34.02450650748714 37.5 33.5 40.0 23.0 41.0 64-65 33.67337478864158 37.0 33.0 40.0 22.0 41.0 66-67 33.34490096941218 36.5 33.0 39.5 22.0 41.0 68-69 32.898572863654095 36.0 32.0 39.0 20.5 41.0 70-71 32.50430188762789 35.5 32.0 39.0 19.5 40.0 72-73 32.08529747919624 35.0 31.5 38.5 18.0 40.0 74-75 31.646313992135113 35.0 31.0 37.5 15.5 39.5 76-77 31.176572492825574 35.0 31.0 37.0 12.0 39.0 78-79 30.69731121645593 35.0 30.5 36.0 7.5 39.0 80-81 30.248207431514338 34.0 30.0 36.0 7.0 38.0 82-83 29.851764064502024 34.0 29.5 35.5 4.0 37.0 84-85 29.479691603501287 34.0 29.0 35.0 2.0 37.0 86-87 29.12611352334144 34.0 29.0 35.0 2.0 36.0 88-89 28.76459860579544 34.0 29.0 35.0 2.0 36.0 90-91 28.388381997658797 34.0 29.0 35.0 2.0 36.0 92-93 27.952745653142493 34.0 27.5 35.0 2.0 35.0 94-95 27.48764089408418 34.0 27.0 35.0 2.0 35.0 96-97 26.90208189775649 33.5 25.5 35.0 2.0 35.0 98-99 26.17734182364613 33.0 24.5 35.0 2.0 35.0 100-101 23.670488414375967 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5952147224332975 1101 2 0.473182107749178 1101 3 0.4574256774737364 1101 4 0.6047419604305944 1101 5 0.5830564591058902 1101 6 0.47051284673371896 1101 7 0.46174315474069516 1101 8 0.3837063286015123 1101 9 0.4422376304361464 1101 10-11 0.5112923347266545 1101 12-13 0.7502392517807195 1101 14-15 0.7469186020486092 1101 16-17 0.7710729477891292 1101 18-19 1.0593359122117079 1101 20-21 0.7824660204896219 1101 22-23 0.8261803783522979 1101 24-25 0.5302695792625443 1101 26-27 0.8307625928966687 1101 28-29 0.5080643606307831 1101 30-31 0.5680310860339617 1101 32-33 0.4581835030419299 1101 34-35 0.559724116050063 1101 36-37 0.5445655177557214 1101 38-39 0.5022971350740022 1101 40-41 0.3207262917385023 1101 42-43 0.51466834826374 1101 44-45 0.2990108223416925 1101 46-47 0.4518804385174988 1101 48-49 0.41705582232817306 1101 50-51 0.5173115526267011 1101 52-53 0.7912415097754462 1101 54-55 0.5444697577775557 1101 56-57 0.6527911231641141 1101 58-59 0.5982942990179865 1101 60-61 0.5647752209371006 1101 62-63 0.5725982041647057 1101 64-65 0.17256885042806402 1101 66-67 0.21137121529449132 1101 68-69 0.5162730372202304 1101 70-71 0.3646101998650835 1101 72-73 0.6801354564868838 1101 74-75 0.3323113658510657 1101 76-77 0.5432964568254199 1101 78-79 0.6493725586784613 1101 80-81 0.4130997232939748 1101 82-83 0.6432533482601812 1101 84-85 0.5815698686104334 1101 86-87 0.5549358967975593 1101 88-89 0.7480627160705602 1101 90-91 0.7210572587612027 1101 92-93 0.5655267620269946 1101 94-95 0.8308827151032112 1101 96-97 0.6891973371340825 1101 98-99 0.47987842133263925 1101 100-101 0.8433094530386427 1102 1 0.03278081197469618 1102 2 0.22001548268393734 1102 3 0.1863757330683704 1102 4 -0.03630276488652129 1102 5 0.23901189263059308 1102 6 0.10720757545458781 1102 7 0.23938049618252677 1102 8 0.1398773331625378 1102 9 0.15549638375480868 1102 10-11 0.1589165149894214 1102 12-13 0.0467197709665399 1102 14-15 0.13229267452586413 1102 16-17 -0.0823330745848736 1102 18-19 -0.036004349650376355 1102 20-21 0.027594115068581004 1102 22-23 -0.2692540540151285 1102 24-25 0.08263178398694748 1102 26-27 -0.2983871495526742 1102 28-29 -0.26803675353109213 1102 30-31 -0.32774145248517783 1102 32-33 -0.23186505717045947 1102 34-35 -0.5114358830422674 1102 36-37 -0.5089834622529068 1102 38-39 -0.7605429566004176 1102 40-41 -0.4919663039636859 1102 42-43 -0.9225448855287155 1102 44-45 -0.42044049264162453 1102 46-47 -0.04128709421525656 1102 48-49 0.25912848111325815 1102 50-51 -0.3018935575414403 1102 52-53 -0.3789302663029801 1102 54-55 -0.40974181952167754 1102 56-57 -0.45489682074202875 1102 58-59 -0.4816734787850052 1102 60-61 -0.10782683492983125 1102 62-63 -0.20811499559022195 1102 64-65 -0.13314491661906658 1102 66-67 -0.40585858343023773 1102 68-69 -0.15919497884549116 1102 70-71 -0.39142125081453116 1102 72-73 -0.20215368100205566 1102 74-75 -0.40131658123191727 1102 76-77 -0.41231292845675327 1102 78-79 -0.3053575236923649 1102 80-81 -0.06520021543846966 1102 82-83 -0.5000560075227938 1102 84-85 -0.18238438494648435 1102 86-87 -0.36439855599238413 1102 88-89 -0.5380817392154675 1102 90-91 -0.07307353612862855 1102 92-93 0.11758830188494684 1102 94-95 0.1650042064009476 1102 96-97 0.07516479725344283 1102 98-99 0.3747171968891543 1102 100-101 0.3861615713077242 1103 1 0.06039325025042075 1103 2 0.1487890046377629 1103 3 0.22550558759926886 1103 4 -0.026386843359766488 1103 5 0.10116323388242421 1103 6 0.1518492410091028 1103 7 0.18886668329049172 1103 8 -0.08016804252142862 1103 9 0.13682562437939083 1103 10-11 -0.10466282572857466 1103 12-13 -0.04832226827007702 1103 14-15 0.2195874830137896 1103 16-17 0.3975614936788361 1103 18-19 0.25070991699849543 1103 20-21 0.4425280534110243 1103 22-23 0.15077130298472952 1103 24-25 0.08874415553373183 1103 26-27 0.2792386345124527 1103 28-29 0.1762712672883353 1103 30-31 0.3564304412823418 1103 32-33 0.4612485154973598 1103 34-35 0.26034444526415257 1103 36-37 0.2725926754756429 1103 38-39 0.3184574437732621 1103 40-41 0.4463160929400871 1103 42-43 0.6202684068775284 1103 44-45 0.65073260224694 1103 46-47 0.5063106636921262 1103 48-49 1.1091551726920628 1103 50-51 1.225705534944879 1103 52-53 1.11766655739914 1103 54-55 1.2474248693879773 1103 56-57 1.4855342482556964 1103 58-59 1.3174390262196667 1103 60-61 1.2232888730642912 1103 62-63 1.231969082883019 1103 64-65 1.4162678686471821 1103 66-67 1.3154546422470688 1103 68-69 1.2776660914868216 1103 70-71 1.3998639487050113 1103 72-73 1.4318914277661214 1103 74-75 1.3891545251340212 1103 76-77 1.0985839085911628 1103 78-79 1.2045183604661815 1103 80-81 1.35242957236348 1103 82-83 1.136498109653239 1103 84-85 0.8181961568925651 1103 86-87 0.8960778886893195 1103 88-89 0.5819903280473113 1103 90-91 0.9146683563043077 1103 92-93 0.9018135788100743 1103 94-95 0.7695751392716268 1103 96-97 0.9885306053409906 1103 98-99 1.180342447122701 1103 100-101 1.4837993665833125 1104 1 0.37279354935311204 1104 2 0.5373296327534156 1104 3 0.5663954181077422 1104 4 0.5220054796711437 1104 5 0.5527335230150285 1104 6 0.6015626009293484 1104 7 0.6468115985447298 1104 8 0.5929314091236364 1104 9 0.5408136602717164 1104 10-11 0.6598530017888677 1104 12-13 0.7308371285394912 1104 14-15 0.7552648259848738 1104 16-17 0.9027584029510152 1104 18-19 0.7045670954631049 1104 20-21 0.8819161391538017 1104 22-23 0.7619004156467426 1104 24-25 0.7618872263213703 1104 26-27 0.26955518486141017 1104 28-29 0.2900268754638091 1104 30-31 0.4845211690988904 1104 32-33 0.3055437547795137 1104 34-35 0.43014442033892664 1104 36-37 0.4489137123649769 1104 38-39 0.65510375484007 1104 40-41 0.7408107340168542 1104 42-43 0.7801979931187972 1104 44-45 0.9565931456168286 1104 46-47 1.0424148511298128 1104 48-49 0.6598406163610591 1104 50-51 0.7918072298601331 1104 52-53 0.7578460190143019 1104 54-55 1.0623488474537695 1104 56-57 0.9769350458628807 1104 58-59 1.0852730242256499 1104 60-61 1.1933007125458488 1104 62-63 0.9683194069109362 1104 64-65 1.232989578517568 1104 66-67 1.3798770001732876 1104 68-69 1.090072621895601 1104 70-71 1.1181621790140923 1104 72-73 1.1249248275667263 1104 74-75 1.422024664715284 1104 76-77 1.421247168810499 1104 78-79 0.9443457532877204 1104 80-81 1.234025409851018 1104 82-83 1.0741102731627095 1104 84-85 1.1950530861049273 1104 86-87 1.1180557052396658 1104 88-89 1.06067926124771 1104 90-91 1.2287386454369091 1104 92-93 1.2147310763175554 1104 94-95 1.3478029558219795 1104 96-97 1.7104429931774803 1104 98-99 1.7415276415394523 1104 100-101 2.0867405430538994 1105 1 0.544447210817701 1105 2 0.5048941585637401 1105 3 0.800323989536313 1105 4 0.7264322503794247 1105 5 0.7273133549478032 1105 6 0.7307492755992158 1105 7 0.917092210789626 1105 8 0.7330799685474005 1105 9 0.980789650667873 1105 10-11 0.9687027917048354 1105 12-13 0.9393117683954344 1105 14-15 1.3167856843282095 1105 16-17 1.2606365542115228 1105 18-19 1.0884131338784684 1105 20-21 1.1260112772090238 1105 22-23 0.949370403641943 1105 24-25 0.945575701711526 1105 26-27 0.8853264933848166 1105 28-29 0.7257524156798922 1105 30-31 0.6395331739008085 1105 32-33 0.9193080104817923 1105 34-35 0.6604828056930643 1105 36-37 0.831326677551047 1105 38-39 0.534853154599972 1105 40-41 0.7770839933205806 1105 42-43 0.7192998838751024 1105 44-45 0.7289408346924588 1105 46-47 1.2014334585727866 1105 48-49 1.4440392958328445 1105 50-51 1.2020113114927895 1105 52-53 1.6296347345004918 1105 54-55 1.614322136769502 1105 56-57 1.3627393675915727 1105 58-59 1.7430011154621425 1105 60-61 1.4555268530020342 1105 62-63 1.509762234041787 1105 64-65 1.7355518534275305 1105 66-67 1.4933861538347557 1105 68-69 1.598558516253341 1105 70-71 1.5311161105867157 1105 72-73 1.4822027387311927 1105 74-75 1.6936858291810672 1105 76-77 1.8035475889785708 1105 78-79 1.8198071878615494 1105 80-81 1.661250049706961 1105 82-83 1.5271414856477001 1105 84-85 1.3807523658168108 1105 86-87 1.8396268336910495 1105 88-89 1.6199529707315001 1105 90-91 1.5469409263492757 1105 92-93 1.6618274148529686 1105 94-95 1.9427159210080518 1105 96-97 2.0177246558425495 1105 98-99 1.9706918072057213 1105 100-101 2.4358401829098426 1106 1 0.1410019617503302 1106 2 0.14097052459402093 1106 3 0.3235190740217213 1106 4 0.3706060489311085 1106 5 0.2764685008771437 1106 6 0.21007778119499676 1106 7 0.19056159854473265 1106 8 0.3733038000154778 1106 9 0.40172447621099394 1106 10-11 0.33659833701973696 1106 12-13 0.4329698768000867 1106 14-15 0.46159586646557926 1106 16-17 0.013171116423499996 1106 18-19 0.4019856596629765 1106 20-21 0.00813767615569816 1106 22-23 0.05134538718375126 1106 24-25 0.02865667603673927 1106 26-27 -0.2573660675105103 1106 28-29 0.04279213479334487 1106 30-31 0.08766791166689103 1106 32-33 -0.024372042184431564 1106 34-35 0.0223546512054682 1106 36-37 0.07200036638142393 1106 38-39 0.2438173854535961 1106 40-41 0.11829531655954639 1106 42-43 0.3265195459334791 1106 44-45 0.22417892993055233 1106 46-47 0.36225197952956023 1106 48-49 0.230104689732336 1106 50-51 -0.45657986691406194 1106 52-53 0.12913001648425393 1106 54-55 0.3737142807238527 1106 56-57 0.5823904538325237 1106 58-59 0.45541059538314954 1106 60-61 0.49639804018658396 1106 62-63 0.2699224271512861 1106 64-65 0.05269032171807453 1106 66-67 -0.03942051722076911 1106 68-69 0.3299168660448686 1106 70-71 -0.13773401010671904 1106 72-73 0.10595740188677283 1106 74-75 0.354800597669108 1106 76-77 0.27635864888640427 1106 78-79 0.6106783750460991 1106 80-81 0.4011447172513982 1106 82-83 0.33035631996852644 1106 84-85 0.7863481525185883 1106 86-87 1.0276342947399826 1106 88-89 0.9134464971743377 1106 90-91 0.8679306125463349 1106 92-93 0.8609715886515197 1106 94-95 1.0011315453222913 1106 96-97 1.035049650356484 1106 98-99 0.9375230558342018 1106 100-101 1.5967025923897644 1107 1 0.3615723217316287 1107 2 0.42996070692221267 1107 3 0.5723736789773071 1107 4 0.3795822060138505 1107 5 0.4456363363142586 1107 6 0.36610863673497107 1107 7 0.39925725071864093 1107 8 0.3510036597630233 1107 9 0.4548801564354008 1107 10-11 0.5932369531955217 1107 12-13 0.5579231259819508 1107 14-15 0.6128815139644104 1107 16-17 0.6154502188078013 1107 18-19 0.6963989368953278 1107 20-21 0.5267211263660769 1107 22-23 0.7648096228078671 1107 24-25 0.21396523143645396 1107 26-27 0.2937559521248332 1107 28-29 0.47772668364795123 1107 30-31 0.5003140079735715 1107 32-33 0.640670033551892 1107 34-35 0.2142316965537603 1107 36-37 0.3580569348713638 1107 38-39 0.7579010694180681 1107 40-41 0.5875099028148085 1107 42-43 0.4963185174154816 1107 44-45 0.7379470458725805 1107 46-47 0.5076002731246945 1107 48-49 0.5999620997370201 1107 50-51 1.0298507081210033 1107 52-53 1.0414137939503618 1107 54-55 0.9970195635663046 1107 56-57 1.060055250466462 1107 58-59 1.131532615018493 1107 60-61 1.0268445104998065 1107 62-63 0.9553478595963583 1107 64-65 1.044602430179971 1107 66-67 1.1666337137282738 1107 68-69 1.0407279927395905 1107 70-71 1.2758587902672858 1107 72-73 1.0544964388199212 1107 74-75 0.7199946135643849 1107 76-77 1.0327401355624168 1107 78-79 1.0943857149245453 1107 80-81 0.998339668163041 1107 82-83 1.2058238281499172 1107 84-85 0.852757689685486 1107 86-87 1.10899240600693 1107 88-89 1.028582074546943 1107 90-91 1.0145762413448374 1107 92-93 1.252310999591213 1107 94-95 1.4564027000675033 1107 96-97 1.891349323100755 1107 98-99 1.4861088435854874 1107 100-101 1.962060236148531 1108 1 0.2417491824538125 1108 2 0.3759076487055566 1108 3 0.34640954240152766 1108 4 0.35440411709221564 1108 5 0.5090728384021972 1108 6 0.2789678850769022 1108 7 0.3464938019345638 1108 8 0.2763291326198143 1108 9 -0.2767336976545067 1108 10-11 0.2656761573222681 1108 12-13 0.01269468088246839 1108 14-15 0.7602654491154794 1108 16-17 0.6933242055432416 1108 18-19 0.810031950896807 1108 20-21 0.8772738161228943 1108 22-23 0.8490763877304985 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0.6958740499043046 2312 22-23 0.5678081235574979 2312 24-25 0.7328557861590994 2312 26-27 1.0419943329344292 2312 28-29 1.0063998470662483 2312 30-31 0.6199167066242453 2312 32-33 0.8305500935219072 2312 34-35 0.6593216515762492 2312 36-37 0.8530719273751188 2312 38-39 0.8524288055500051 2312 40-41 0.8867602776274026 2312 42-43 0.9989796868307721 2312 44-45 0.3583743322294026 2312 46-47 0.370381382569974 2312 48-49 0.47563676240567787 2312 50-51 0.48577274710151386 2312 52-53 -0.7966671655698789 2312 54-55 -0.5979579476780756 2312 56-57 -0.15415014683488693 2312 58-59 -0.14609107313743408 2312 60-61 -0.5322634355271774 2312 62-63 -0.2987001164156382 2312 64-65 -0.6885684843627544 2312 66-67 -0.5498580403946605 2312 68-69 -0.18712058297255396 2312 70-71 -0.16413371348083672 2312 72-73 -0.2570477890661351 2312 74-75 -0.1809798991792455 2312 76-77 -0.37711743565197153 2312 78-79 0.10443069243579117 2312 80-81 0.02817475062181174 2312 82-83 0.2701041879700128 2312 84-85 -0.3879844392500438 2312 86-87 -0.7374843556122599 2312 88-89 -0.48850937972592234 2312 90-91 -0.701915512778104 2312 92-93 -0.23880594193802196 2312 94-95 -0.21140089813339813 2312 96-97 -0.12383058694422289 2312 98-99 0.9331478240952329 2312 100-101 0.39759247003159004 2313 1 0.0882878796153399 2313 2 0.37315085883420807 2313 3 0.1831122855776215 2313 4 0.3966068262338638 2313 5 0.09977462861389341 2313 6 0.2889740112766148 2313 7 0.574898947942323 2313 8 0.2839528478195916 2313 9 0.6138115341200461 2313 10-11 0.43608661270311444 2313 12-13 0.28245167496046975 2313 14-15 0.6559668989827472 2313 16-17 0.5595177934542122 2313 18-19 0.4611448771781994 2313 20-21 0.4074964013791771 2313 22-23 0.4246839025354845 2313 24-25 0.451956326250496 2313 26-27 0.46290210194148784 2313 28-29 0.455922073904631 2313 30-31 0.6663957261506255 2313 32-33 0.7104316888688089 2313 34-35 1.063039973138359 2313 36-37 1.0644346195230199 2313 38-39 0.6282389426501283 2313 40-41 1.06260157384677 2313 42-43 1.1513997117580672 2313 44-45 1.147271299408466 2313 46-47 1.0540643904636156 2313 48-49 0.44780128255523977 2313 50-51 0.8068674708239882 2313 52-53 1.0544441763495271 2313 54-55 0.8440860905437759 2313 56-57 1.3312050671243938 2313 58-59 1.199775150377313 2313 60-61 1.373636024381426 2313 62-63 1.2252404735303486 2313 64-65 1.3281016444282656 2313 66-67 1.7192528860698175 2313 68-69 1.702160680010408 2313 70-71 1.3858247410268447 2313 72-73 2.2572334739168305 2313 74-75 2.291288484701102 2313 76-77 2.182032959030206 2313 78-79 2.012615154421667 2313 80-81 1.7001730888021207 2313 82-83 1.3897552058345717 2313 84-85 1.414622540392667 2313 86-87 1.3770254075784365 2313 88-89 1.7101300054007922 2313 90-91 1.6680990281442085 2313 92-93 1.4920831315975 2313 94-95 1.230643140088457 2313 96-97 1.2765640952327644 2313 98-99 1.8441366068123095 2313 100-101 1.848257197908616 2314 1 -0.6082279132859973 2314 2 -0.2884685919418679 2314 3 -0.4656991764868508 2314 4 -0.5416868891682824 2314 5 -0.17255794492349708 2314 6 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-2.099562917685752 2314 68-69 -1.2917338168961336 2314 70-71 -1.4243076805513581 2314 72-73 -1.6893637256924166 2314 74-75 -1.5565623782099962 2314 76-77 -1.6301074946765155 2314 78-79 -1.3282347316089442 2314 80-81 -0.9124123882411901 2314 82-83 -1.0527445228097356 2314 84-85 -1.2152211587281414 2314 86-87 -1.5181227526299672 2314 88-89 -1.4393869814655922 2314 90-91 -1.3946384080872072 2314 92-93 -1.0156776412235153 2314 94-95 -1.2666975741197284 2314 96-97 -1.6409166385130334 2314 98-99 -2.39723428214894 2314 100-101 -2.698633065849119 2315 1 -0.7202924493049032 2315 2 -1.2027802419949651 2315 3 -0.8539943157325602 2315 4 -1.1996870946970049 2315 5 -0.7029419410869622 2315 6 -0.3462818410299491 2315 7 -0.08168174746287349 2315 8 -0.23744071120739108 2315 9 -0.7889783325710482 2315 10-11 -0.9197817275781617 2315 12-13 -0.7456187817714053 2315 14-15 -0.45820461597442375 2315 16-17 -1.0235025006499612 2315 18-19 -0.9927810928616552 2315 20-21 -1.3943154085983807 2315 22-23 -1.205235012669064 2315 24-25 -1.3965365043876474 2315 26-27 -0.6700975773841762 2315 28-29 -0.8620608845451372 2315 30-31 -0.9061911994987426 2315 32-33 -1.137900804603177 2315 34-35 -1.018926676731084 2315 36-37 -1.1836069765189379 2315 38-39 -0.6746952274905098 2315 40-41 -1.1376595466809718 2315 42-43 -1.6341493285094728 2315 44-45 -1.4208147329338274 2315 46-47 -2.0789925521356807 2315 48-49 -2.386891099138829 2315 50-51 -2.3564817435238936 2315 52-53 -1.8504071681921417 2315 54-55 -1.7570637559906501 2315 56-57 -2.238816463866492 2315 58-59 -2.1854577966197937 2315 60-61 -2.7522057457408877 2315 62-63 -2.702905570722706 2315 64-65 -2.334022135864 2315 66-67 -1.91694208392423 2315 68-69 -2.3046307442417344 2315 70-71 -1.7932146102947897 2315 72-73 -2.182067456038734 2315 74-75 -2.1932389787649242 2315 76-77 -1.5411563762577138 2315 78-79 -2.1481915705771897 2315 80-81 -2.5617033141480867 2315 82-83 -2.1461145087652724 2315 84-85 -1.8841657687373932 2315 86-87 -1.9896313334541347 2315 88-89 -2.299558940496869 2315 90-91 -2.1740375791213573 2315 92-93 -2.2548017463172023 2315 94-95 -3.005487695039129 2315 96-97 -2.569615299150854 2315 98-99 -2.766209721466584 2315 100-101 -3.0998508235307582 2316 1 -5.345081380171411 2316 2 -5.375838709663423 2316 3 -5.275257936524845 2316 4 -6.72293203207477 2316 5 -7.4320202826605986 2316 6 -7.729425465223635 2316 7 -10.319342554809904 2316 8 -10.01022706672677 2316 9 -11.744189158747268 2316 10-11 -10.57543230642387 2316 12-13 -9.88892713099819 2316 14-15 -9.723137377548298 2316 16-17 -8.046346558522387 2316 18-19 -8.323652459131896 2316 20-21 -8.943392778342911 2316 22-23 -9.494712533047107 2316 24-25 -8.622286331280584 2316 26-27 -7.6158634394573355 2316 28-29 -5.782048979638613 2316 30-31 -7.501971031615263 2316 32-33 -5.144345246348095 2316 34-35 -3.2571705815404215 2316 36-37 -3.7908908356523128 2316 38-39 -3.276482178255222 2316 40-41 -2.444654286655954 2316 42-43 -2.696583274500064 2316 44-45 -2.9578231999948983 2316 46-47 -2.809152053587347 2316 48-49 -3.8054032287808326 2316 50-51 -3.6954132174242105 2316 52-53 -2.9559295870747846 2316 54-55 -2.6873046481297607 2316 56-57 -3.4870519866498064 2316 58-59 -3.5931826232090387 2316 60-61 -4.127999912336691 2316 62-63 -3.601677421692713 2316 64-65 -4.135811516201468 2316 66-67 -3.8976877198041606 2316 68-69 -4.397294649294029 2316 70-71 -3.9026486413204324 2316 72-73 -2.448451887336489 2316 74-75 -3.2299498231629364 2316 76-77 -3.31584994639341 2316 78-79 -3.5862435391385183 2316 80-81 -4.483282779399122 2316 82-83 -3.380386062112617 2316 84-85 -3.433552909008771 2316 86-87 -4.300762421052383 2316 88-89 -6.083818953373605 2316 90-91 -5.124425703369706 2316 92-93 -4.540337284892747 2316 94-95 -3.680939291875827 2316 96-97 -5.8435617756629625 2316 98-99 -6.661762388530082 2316 100-101 -7.822909898592407 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 913.0 3 323.0 4 325.0 5 341.0 6 367.0 7 426.0 8 557.0 9 698.0 10 874.0 11 1032.0 12 1339.0 13 1628.0 14 1910.0 15 2285.0 16 2720.0 17 3555.0 18 2883.0 19 2140.0 20 2253.0 21 2355.0 22 2685.0 23 2914.0 24 3476.0 25 3926.0 26 4588.0 27 5307.0 28 6257.0 29 7368.0 30 9148.0 31 11384.0 32 14574.0 33 18406.0 34 24725.0 35 34141.0 36 48882.0 37 67528.0 38 57947.0 39 9169.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.73660217245601 18.854447663308093 14.443896683708795 22.965053480527107 2 15.101192272237801 19.481346351347984 41.72011637595745 23.697345000456757 3 19.434026499561295 24.138293720539515 30.15342288306591 26.274256896833283 4 12.304238794072221 16.16880996008658 38.098493697444106 33.42845754839709 5 13.25676734291654 37.11180683004066 35.12430208964809 14.507123737394709 6 31.437588240179387 35.868283365169006 18.410707859258643 14.283420535392963 7 28.436662161263875 32.19378032837739 21.25202189182602 18.117535618532717 8 26.040931154694956 34.452280675521884 20.409366926674473 19.097421243108688 9 25.853335659668275 16.635510395116913 20.94449198660696 36.56666195860785 10-11 24.430186610250686 26.34516388971193 28.255295228548405 20.969354271488978 12-13 25.510173006165545 23.671169238180404 28.715590488398423 22.10306726725563 14-15 22.340437312779965 25.227432545417294 26.945980963771365 25.486149178031376 16-17 21.627776270398076 28.532528946304677 27.586202123005876 22.253492660291375 18-19 21.446142047727616 26.970850965751236 30.11734642319741 21.46566056332374 20-21 22.09096344190784 26.184512079006222 30.249966785500764 21.474557693585172 22-23 21.75217513447984 26.16098278574977 30.169983628540862 21.91685845122952 24-25 21.817165191299488 26.16977517950762 30.207243416208556 21.80581621298434 26-27 21.451823186985656 26.542297439815314 30.1576690665803 21.848210306618725 28-29 21.268290117870418 26.637496575143427 30.323610892012542 21.77060241497361 30-31 21.40949608273442 26.315279688057807 30.197260819872202 22.077963409335574 32-33 21.159644910639535 26.491105468182415 30.233381997827397 22.115867623350656 34-35 21.834574188370098 26.667266336387037 29.69790484625391 21.80025462898896 36-37 21.768832678363868 26.320639840593348 29.87864615044003 22.031881330602758 38-39 21.48559393452128 26.591129555647502 29.966498165771117 21.9567783440601 40-41 21.60096640818292 26.58867874400146 29.936790484255738 21.87356436355988 42-43 21.466099713156208 26.638282092119336 29.675394669659177 22.22022352506528 44-45 21.513312467310357 26.724006719191486 29.59516041101084 22.167520402487316 46-47 21.568225907773883 26.952326131655923 29.10065282785989 22.3787951327103 48-49 21.737957296191084 27.112117216157767 28.883255684593443 22.266669803057702 50-51 21.615697865080385 27.220259666222496 28.86064284202093 22.30339962667619 52-53 21.76434368103168 27.27516759950464 28.737590026359307 22.22289869310438 54-55 21.54070950012868 26.98635406375483 28.964060074221052 22.50887636189543 56-57 21.562655670559582 27.344191066585488 28.70578956107821 22.38736370177672 58-59 21.762070282447098 26.958117240730388 28.88081097685849 22.399001499964022 60-61 21.68899602305906 26.892783111423533 29.29403071405047 22.12419015146693 62-63 21.476902895855474 26.967654091392134 29.13428754870705 22.42115546404534 64-65 21.364407459782868 27.00490658667058 29.074224230734107 22.556461722812447 66-67 21.29565826214256 27.097464252406926 28.841603639654302 22.765273845796216 68-69 21.30312775013422 26.9669513430341 29.115031022897718 22.61488988393396 70-71 21.344945488459103 26.921879475460464 28.96839728182584 22.764777754254585 72-73 21.424360269593258 26.84155168357391 28.939479911980847 22.794608134851988 74-75 21.205763953564 26.998639689245795 28.893803044162357 22.901793313027845 76-77 21.32007656228159 26.984744287238648 28.875550308698788 22.819628841780972 78-79 21.3792044020283 26.76742654059919 28.76666260711676 23.08670645025575 80-81 21.22483999308078 26.974295104653173 28.85881335409099 22.94205154817506 82-83 21.2669007039106 27.21464776103294 28.730816118404213 22.78763541665225 84-85 21.310579392490258 26.86691905773999 28.458062123627347 23.364439426142404 86-87 21.293412278796115 26.71327935928534 28.75439501973867 23.238913342179874 88-89 21.314741862772365 26.359871426060995 28.82895598016873 23.496430730997915 90-91 21.161244077403357 26.284151795598458 29.3334587964398 23.221145330558386 92-93 21.14981975686204 26.2713541414407 29.177939070208335 23.400887031488928 94-95 20.652172408878116 26.287275248363272 29.371837078675007 23.68871526408361 96-97 20.482216151736317 26.310297776707603 29.60067642557243 23.606809645983653 98-99 20.638011642487605 26.864223571193286 28.816125648073566 23.681639138245544 100-101 20.427198064557203 27.052186646219766 28.616983289090157 23.90363200013287 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 19.0 1 17.5 2 23.0 3 35.5 4 34.5 5 34.5 6 57.5 7 109.0 8 185.0 9 249.5 10 244.5 11 226.0 12 256.5 13 294.0 14 317.0 15 348.5 16 381.5 17 419.0 18 470.5 19 524.0 20 602.5 21 688.0 22 798.0 23 938.5 24 1124.5 25 1396.5 26 1822.0 27 2405.5 28 2988.0 29 3640.5 30 4523.5 31 5410.0 32 6391.0 33 7275.0 34 8052.0 35 8953.5 36 9893.5 37 10695.0 38 11124.0 39 11346.0 40 11583.5 41 12264.0 42 12955.5 43 13250.5 44 13471.0 45 13861.5 46 14046.5 47 14256.0 48 15320.0 49 15137.5 50 13725.0 51 12850.0 52 12095.5 53 11517.0 54 10882.5 55 9875.0 56 8820.5 57 7782.5 58 6726.0 59 5771.0 60 4852.0 61 3876.0 62 3026.0 63 2378.5 64 1739.5 65 1250.5 66 941.0 67 746.0 68 561.0 69 362.0 70 259.5 71 212.0 72 174.5 73 136.5 74 91.5 75 73.0 76 59.0 77 40.0 78 24.5 79 14.0 80 13.5 81 10.0 82 5.0 83 3.0 84 2.0 85 1.5 86 1.0 87 1.0 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07942372140261739 2 0.030441147575916074 3 0.01771121313507844 4 0.019648377071727646 5 0.02656681970261766 6 0.03403873774397888 7 0.0844050000968582 8 0.05811491809947614 9 0.07471918041361217 10-11 0.06254272138324575 12-13 0.042064131195811295 14-15 0.0406804426696333 16-17 0.04621519677434531 18-19 0.0435861885746071 20-21 0.017987950840314043 22-23 0.014805467230104636 24-25 0.024076180355497256 26-27 0.024906393471204057 28-29 0.006641704925654415 30-31 0.0011069508209424026 32-33 0.009824188535863823 34-35 0.011899721325130829 36-37 0.0035975901680628084 38-39 0.008163762304450218 40-41 0.0041510655785340096 42-43 0.0017987950840314042 44-45 0.0 46-47 5.534754104712013E-4 48-49 9.685819683246023E-4 50-51 0.001245319673560203 52-53 1.3836885261780032E-4 54-55 0.0 56-57 0.0035975901680628084 58-59 0.003044114757591607 60-61 0.005534754104712013 62-63 0.002490639347120406 64-65 5.534754104712013E-4 66-67 0.0 68-69 0.0019371639366492046 70-71 0.0020755327892670048 72-73 0.018818163956020845 74-75 0.010654401651570625 76-77 0.02172390986099465 78-79 0.017987950840314043 80-81 0.011207877062041826 82-83 0.004012696725916209 84-85 0.002490639347120406 86-87 0.0017987950840314042 88-89 0.001245319673560203 90-91 0.006918442630890016 92-93 0.009824188535863823 94-95 0.010377663946335023 96-97 0.011622983619895227 98-99 0.024906393471204057 100-101 0.025459868881675257 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 361353.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.63543251643839 #Duplication Level Percentage of deduplicated Percentage of total 1 89.8874043887591 70.68334921890073 2 6.5626494958546155 10.32113563120628 3 1.5280877266706878 3.6048551792943195 4 0.633224436528424 1.9917550978556247 5 0.34882461320621605 1.371498716592506 6 0.21956850631215113 1.0359518676506603 7 0.1589409109861686 0.8748871095967871 8 0.10186928704054593 0.6408428357259633 9 0.08543971874559515 0.6046730313878451 >10 0.441214758224104 6.076409139627067 >50 0.024670226206815006 1.3155658858479466 >100 0.007401067859850339 0.9425404627609081 >500 3.524318028500161E-4 0.2344572865321165 >1k 3.524318028500161E-4 0.3020785370212849 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1090 0.3016440987068047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 846 0.23412009862931815 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 492 0.1361549509759155 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.3836885261780032E-4 0.0 22-23 0.0 0.0 0.0 4.15106557853401E-4 0.0 24-25 0.0 0.0 0.0 0.0013836885261780032 0.0 26-27 0.0 0.0 0.0 0.002213901641884805 0.0 28-29 0.0 0.0 0.0 0.004704540989005211 0.0 30-31 0.0 0.0 0.0 0.007195180336125617 0.0 32-33 0.0 0.0 0.0 0.013283409851308832 0.0 34-35 0.0 0.0 0.0 0.020478590187434448 0.0 36-37 0.0 0.0 0.0 0.025044762323821856 0.0 38-39 0.0 0.0 0.0 0.03293178692303648 0.0 40-41 0.0 0.0 0.0 0.041233918080104494 0.0 42-43 0.0 0.0 0.0 0.06475662302513055 0.0 44-45 0.0 0.0 0.0 0.09049322961204141 0.0 46-47 0.0 0.0 0.0 0.12010416407225068 0.0 48-49 0.0 0.0 0.0 0.15345105755314056 0.0 50-51 0.0 0.0 0.0 0.19247107399136026 0.0 52-53 0.0 0.0 0.0 0.2616555003002604 0.0 54-55 0.0 0.0 0.0 0.31188339380052194 2.7673770523560065E-4 56-57 0.0 0.0 0.0 0.41621350867434337 2.7673770523560065E-4 58-59 0.0 0.0 0.0 0.5590101645759133 2.7673770523560065E-4 60-61 0.0 0.0 0.0 0.6997312876882162 2.7673770523560065E-4 62-63 0.0 0.0 0.0 0.907838042025388 2.7673770523560065E-4 64-65 0.0 0.0 0.0 1.035967599549471 2.7673770523560065E-4 66-67 0.0 0.0 0.0 1.2846164277036582 2.7673770523560065E-4 68-69 0.0 0.0 0.0 1.5677191001596777 2.7673770523560065E-4 70-71 0.0 0.0 0.0 1.7700143626869016 2.7673770523560065E-4 72-73 0.0 0.0 0.0 2.1361383467136017 2.7673770523560065E-4 74-75 0.0 0.0 0.0 2.477909412679568 2.7673770523560065E-4 76-77 0.0 0.0 0.0 2.7016518473625513 2.7673770523560065E-4 78-79 0.0 0.0 0.0 2.9413067000965816 2.7673770523560065E-4 80-81 0.0 0.0 0.0 3.3987541268510295 2.7673770523560065E-4 82-83 0.0 0.0 0.0 3.7067631927782525 2.7673770523560065E-4 84-85 0.0 0.0 0.0 4.183028783488721 2.7673770523560065E-4 86-87 0.0 0.0 0.0 5.069142915653115 2.7673770523560065E-4 88-89 0.0 0.0 0.0 6.233378441579287 2.7673770523560065E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGTAC 50 0.001606651 38.03698 3 ATACGTA 50 0.001606651 38.03698 7 GTACTAG 100 1.2888977E-6 33.273132 1 GGTATCA 1085 0.0 31.98076 1 CTACACG 60 0.0039286404 31.697483 4 TGTACCG 60 0.0039286404 31.697483 5 GTACATG 1425 0.0 30.354437 1 GTATCAA 1620 0.0 29.6348 1 TACATGG 1440 0.0 28.721865 2 ACATGGG 1465 0.0 28.235641 3 ACTATAC 85 6.3623075E-4 27.968367 3 ATAGACG 70 0.008333111 27.176804 8 GTATAGG 105 6.860371E-5 27.161741 1 AATAGAC 130 9.921083E-6 25.601814 7 GTCCTAG 225 1.0004442E-10 25.350958 1 CCAATAC 115 1.2709513E-4 24.806726 3 TATAAGG 115 1.2721482E-4 24.803288 2 ATCAACG 1945 0.0 24.44536 3 TCAACGC 1955 0.0 24.320318 4 CAACGCA 1950 0.0 24.138851 5 >>END_MODULE