FastQCFastQC Report
Thu 26 May 2016
SRR936540_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936540_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361353
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13650.3777469676465949No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12540.3470290823654432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.19316291825444926No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4510.1248087050612559No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.11041834438900466No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG550.002575766834.5496331
TAAGGTG1255.638867E-934.194675
GTAAGGT1351.1941665E-831.661734
GGCGCAT404.4815542E-429.69931288-89
GGTATCA16200.029.617781
GTACGTT350.00837328127.15365694-95
ATGGGTA1754.945832E-927.1386285
CCTATAC700.00839046927.1386263
TCGCGGT458.9524616E-426.38477726-27
GTACATG17700.026.3026141
GTATCAA22700.026.1595521
TACATGG17950.025.1388342
GGTAAGG1751.4825127E-724.4247653
ACATGGG17950.023.8124453
TGCGTAA601.7069558E-423.7561660-61
CATGGGT2853.6379788E-1223.3296994
GGGTAGG1852.5286863E-723.1109031
GTTGTAG1652.4196797E-623.0330891
ACTATAC1050.002195317522.615523
CTATACC1050.002195317522.615524
Produced by FastQC (version 0.11.5)