##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936540_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 361353 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03561337528677 31.0 31.0 34.0 30.0 34.0 2 32.20345479351216 33.0 31.0 34.0 30.0 34.0 3 32.282015093274445 34.0 31.0 34.0 30.0 34.0 4 35.959388741756676 37.0 35.0 37.0 35.0 37.0 5 35.81191521863662 37.0 35.0 37.0 35.0 37.0 6 35.9033355195612 37.0 35.0 37.0 35.0 37.0 7 35.85189274753496 37.0 35.0 37.0 35.0 37.0 8 35.814367114705014 37.0 35.0 37.0 35.0 37.0 9 37.48743472449378 39.0 37.0 39.0 35.0 39.0 10-11 37.38097372928964 39.0 37.0 39.0 34.0 39.0 12-13 37.28325764557095 39.0 37.0 39.0 34.0 39.0 14-15 38.45790127659103 40.0 38.0 41.0 34.0 41.0 16-17 38.36643116287951 40.0 38.0 41.0 34.0 41.0 18-19 38.49492739786303 40.0 38.0 41.0 34.0 41.0 20-21 38.512938871408295 40.0 38.0 41.0 34.0 41.0 22-23 38.44361054149267 40.0 38.0 41.0 34.0 41.0 24-25 38.30887663863314 40.0 38.0 41.0 34.0 41.0 26-27 38.131137696379994 40.0 37.5 41.0 33.0 41.0 28-29 38.049655046450425 40.0 37.5 41.0 33.0 41.0 30-31 38.02898135618079 40.0 37.0 41.0 33.0 41.0 32-33 37.88131688404414 40.0 37.0 41.0 33.0 41.0 34-35 37.72242516320606 40.0 37.0 41.0 33.0 41.0 36-37 37.52791453232712 40.0 37.0 41.0 33.0 41.0 38-39 37.35323077433978 40.0 36.0 41.0 32.0 41.0 40-41 37.176064679136466 39.5 36.0 41.0 31.5 41.0 42-43 36.91983047048177 39.0 36.0 41.0 31.0 41.0 44-45 36.59969614199965 39.0 36.0 41.0 30.5 41.0 46-47 36.19385337882901 39.0 35.0 40.5 30.0 41.0 48-49 35.79533032796185 39.0 35.0 40.0 28.5 41.0 50-51 35.52087017404034 39.0 35.0 40.0 27.5 41.0 52-53 35.169494926014174 38.0 34.5 40.0 27.0 41.0 54-55 34.983666940636994 38.0 34.0 40.0 26.5 41.0 56-57 34.69603407194627 38.0 34.0 40.0 26.0 41.0 58-59 34.34187069153985 38.0 34.0 40.0 25.5 41.0 60-61 33.94337808182027 37.0 33.0 40.0 24.0 41.0 62-63 33.62903587350873 37.0 33.0 39.5 23.0 41.0 64-65 33.204406771218174 36.0 32.0 39.0 22.5 40.0 66-67 32.80639430141717 36.0 32.0 39.0 22.0 40.0 68-69 32.45901376216608 35.0 31.0 38.5 21.0 40.0 70-71 32.109404100699315 35.0 31.0 38.0 20.0 40.0 72-73 31.65778892108271 35.0 31.0 37.5 18.5 39.5 74-75 31.147101310906507 34.5 30.5 37.0 17.5 39.0 76-77 29.6445954509856 33.0 28.5 35.0 13.5 37.5 78-79 30.40158100251001 34.0 30.0 36.0 13.5 38.0 80-81 30.157820745918812 34.0 30.0 35.5 8.0 37.0 82-83 29.86775950386464 34.0 30.0 35.0 7.0 37.0 84-85 29.50276045860973 34.0 29.0 35.0 4.5 36.5 86-87 29.124897261126932 34.0 29.0 35.0 2.0 36.0 88-89 28.686327496935128 34.0 29.0 35.0 2.0 35.5 90-91 28.227384302883884 33.0 28.5 35.0 2.0 35.0 92-93 27.54033036947251 33.0 26.5 35.0 2.0 35.0 94-95 27.072795853362223 33.0 26.0 34.5 2.0 35.0 96-97 26.458272105116052 32.0 25.0 34.0 2.0 35.0 98-99 25.649311061482816 32.0 24.0 34.0 2.0 35.0 100-101 22.57406469574073 28.5 8.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.37790602866584067 1101 2 0.22497545617813586 1101 3 0.1711682566023498 1101 4 0.1828681002880046 1101 5 0.26101179365423377 1101 6 0.2807258905470462 1101 7 0.3655121883374761 1101 8 0.2995459651407941 1101 9 0.3019495418054774 1101 10-11 0.4016809067549971 1101 12-13 0.4731663195643492 1101 14-15 0.6514445885181388 1101 16-17 0.5159373152978262 1101 18-19 0.3529109434658366 1101 20-21 0.46628635049221856 1101 22-23 0.3358416055177713 1101 24-25 0.3657660938363776 1101 26-27 0.330204792561112 1101 28-29 0.25366733112914375 1101 30-31 0.5446651625754058 1101 32-33 0.5060063826310639 1101 34-35 0.5680264478750345 1101 36-37 0.36807114013184616 1101 38-39 0.4723201421822836 1101 40-41 0.640747893181647 1101 42-43 0.7524848766711543 1101 44-45 0.6420230782141445 1101 46-47 0.6516169741963296 1101 48-49 0.734002338949054 1101 50-51 0.5349403908755264 1101 52-53 0.6607641458588773 1101 54-55 0.5766120200862019 1101 56-57 0.718089156138852 1101 58-59 0.5036590842866744 1101 60-61 0.49249609257110905 1101 62-63 0.4109047298831854 1101 64-65 0.42646925152226345 1101 66-67 0.5699667528936061 1101 68-69 0.3753343757311498 1101 70-71 0.41711215492284026 1101 72-73 0.39552708317538077 1101 74-75 0.17577792837198558 1101 76-77 -0.03438128668755169 1101 78-79 0.38483225461510884 1101 80-81 0.4634109017913204 1101 82-83 0.5368338404523918 1101 84-85 0.3466653583377486 1101 86-87 0.547952618414957 1101 88-89 0.6229717259515901 1101 90-91 0.6642648846539849 1101 92-93 0.5242398151554291 1101 94-95 0.4569488556908077 1101 96-97 0.37780160285199216 1101 98-99 0.10583932764341242 1101 100-101 0.10459988320662106 1102 1 0.16199007867832194 1102 2 0.07613840518991566 1102 3 0.04785935465313429 1102 4 0.09956237517511113 1102 5 0.23805915120716037 1102 6 -0.038838882876447656 1102 7 0.07579833052359675 1102 8 0.1690982581358682 1102 9 0.23630702665877834 1102 10-11 0.07497165535378514 1102 12-13 0.08325164031136723 1102 14-15 0.17864965330291227 1102 16-17 0.24916588867140632 1102 18-19 0.08459988580625577 1102 20-21 -0.1272034042106327 1102 22-23 -0.19111952362062823 1102 24-25 -0.0556888972004046 1102 26-27 0.02560859114969105 1102 28-29 0.12003711487735558 1102 30-31 0.022299100264028482 1102 32-33 -0.06475651192067033 1102 34-35 -0.0034784648754282443 1102 36-37 0.12437942940547941 1102 38-39 0.20995430678778604 1102 40-41 0.25057793243826865 1102 42-43 0.10067861830359703 1102 44-45 0.22452097923300585 1102 46-47 0.33715556141183356 1102 48-49 0.4310444774137352 1102 50-51 0.05188427433102305 1102 52-53 -0.018063374620368222 1102 54-55 -0.029932262742995874 1102 56-57 -0.2061784696298261 1102 58-59 0.07083555754451965 1102 60-61 0.23098459923112102 1102 62-63 0.08254919651506043 1102 64-65 -0.05327841690679236 1102 66-67 -0.2690765655388532 1102 68-69 -0.24018560430016578 1102 70-71 -0.04233779855920261 1102 72-73 0.2751794465146915 1102 74-75 -0.06389962274104022 1102 76-77 -0.18661755254336754 1102 78-79 0.05799592976090295 1102 80-81 0.21692244051468634 1102 82-83 0.14424381118011098 1102 84-85 0.3334247583695209 1102 86-87 0.34054378227163795 1102 88-89 0.19881642403868227 1102 90-91 0.26926159435921804 1102 92-93 -0.025956467946070205 1102 94-95 -0.15347230203911622 1102 96-97 0.47363654351322637 1102 98-99 0.3722283993264561 1102 100-101 0.5685064842186662 1103 1 -0.030456204519328622 1103 2 0.11161151342426479 1103 3 0.07796745554730222 1103 4 0.07776870107929312 1103 5 0.1114343013472947 1103 6 0.01841455366432143 1103 7 -0.12994035891988887 1103 8 -0.0897032899757022 1103 9 0.13386474719794705 1103 10-11 0.14592454471719662 1103 12-13 0.06123108779568298 1103 14-15 -0.078643820606068 1103 16-17 0.12610436458225394 1103 18-19 0.028801140310463325 1103 20-21 0.0716421850059703 1103 22-23 -0.09178014019609293 1103 24-25 0.08142574313057338 1103 26-27 0.06441814673490143 1103 28-29 -0.1682786464999282 1103 30-31 -0.02986959051403204 1103 32-33 -0.08069805736307245 1103 34-35 0.034223390189296765 1103 36-37 -0.1549079054403677 1103 38-39 -0.052217053148154946 1103 40-41 0.12054323338582407 1103 42-43 0.31090816588133663 1103 44-45 0.1854778723332302 1103 46-47 0.15048133213117865 1103 48-49 0.21329457750714909 1103 50-51 -0.1525064903827129 1103 52-53 -0.2501599981356506 1103 54-55 -0.13430968166733237 1103 56-57 -0.32242029533382066 1103 58-59 -0.03968092450539018 1103 60-61 -0.12937173334595542 1103 62-63 -0.04323486861239445 1103 64-65 -0.03302618148706671 1103 66-67 0.12492450212430128 1103 68-69 -0.26017092393181684 1103 70-71 -0.22270614234673758 1103 72-73 0.021302494692992724 1103 74-75 -0.0887094452420385 1103 76-77 -0.0026858829851157395 1103 78-79 -0.04921746941166205 1103 80-81 0.04426129010763802 1103 82-83 -0.1963073699222484 1103 84-85 0.41289893426702307 1103 86-87 0.29294602436425876 1103 88-89 0.3261686194210398 1103 90-91 0.4342155513857797 1103 92-93 0.2221150654851307 1103 94-95 0.07165114651286686 1103 96-97 0.4022899533624198 1103 98-99 0.4963976239360939 1103 100-101 0.5523847706193088 1104 1 0.25212729498216646 1104 2 0.35933085539834764 1104 3 0.27908909064201737 1104 4 0.2563392394641326 1104 5 0.43763071211499494 1104 6 0.10155887071317693 1104 7 0.2285716051877884 1104 8 0.08004371899738061 1104 9 0.2597495926615565 1104 10-11 0.2268287072296573 1104 12-13 0.3439018255823214 1104 14-15 0.38902193213570513 1104 16-17 0.42799868163110943 1104 18-19 0.27602820910408354 1104 20-21 0.2872173345573117 1104 22-23 0.33445365840809416 1104 24-25 0.4130278119241879 1104 26-27 0.19359000615664002 1104 28-29 0.3762596137194123 1104 30-31 0.24550428785087064 1104 32-33 0.2724207113308452 1104 34-35 0.2742875726419385 1104 36-37 0.3679890287570373 1104 38-39 0.32694857496750274 1104 40-41 0.30549774485142933 1104 42-43 0.579486804964084 1104 44-45 0.7872569102195754 1104 46-47 0.7555567309347566 1104 48-49 0.9653789493914999 1104 50-51 0.7913556731267519 1104 52-53 0.9334984016649415 1104 54-55 1.134895203676642 1104 56-57 1.2941269379662756 1104 58-59 1.0329013287348872 1104 60-61 1.125815828967113 1104 62-63 0.9597000765521102 1104 64-65 1.2728997905966892 1104 66-67 1.1763857434004734 1104 68-69 0.8968237171449474 1104 70-71 0.9680185585505683 1104 72-73 1.2011535664776396 1104 74-75 0.9530309585465915 1104 76-77 0.9749343812222655 1104 78-79 0.8692178496312124 1104 80-81 0.9237166739560969 1104 82-83 1.0450529241953959 1104 84-85 1.317778046929039 1104 86-87 1.4980183075148084 1104 88-89 1.3366652545157542 1104 90-91 1.1608824257626473 1104 92-93 1.6821505839298005 1104 94-95 1.647155882305487 1104 96-97 1.541974026144075 1104 98-99 1.4530962779739802 1104 100-101 1.667788559253406 1105 1 0.1876690116688451 1105 2 0.1858764736456422 1105 3 0.14358488896134247 1105 4 0.14743317703916858 1105 5 0.28850106025424793 1105 6 0.3091444049268617 1105 7 0.187320104587549 1105 8 0.30853261455560244 1105 9 0.25268176553070276 1105 10-11 0.2401652918635122 1105 12-13 0.42468663950788965 1105 14-15 0.1890144291345095 1105 16-17 0.5515280933958095 1105 18-19 0.4316029389960363 1105 20-21 0.40856712447327936 1105 22-23 0.5716235263552676 1105 24-25 0.4658601948773722 1105 26-27 0.3685862546560301 1105 28-29 0.6621952379691152 1105 30-31 0.6661525471545957 1105 32-33 0.4936879682336013 1105 34-35 0.5973330408292128 1105 36-37 0.767212468132783 1105 38-39 0.7140921824104822 1105 40-41 0.8638673426905612 1105 42-43 0.8447891759124673 1105 44-45 0.9181655236649462 1105 46-47 0.6829614428195185 1105 48-49 0.5694343215403634 1105 50-51 0.43405525295869296 1105 52-53 0.6899472311967614 1105 54-55 0.5548982048771194 1105 56-57 0.22252954901069444 1105 58-59 0.46801537435313634 1105 60-61 0.6804864472144132 1105 62-63 0.47400829984142945 1105 64-65 0.7228022515810792 1105 66-67 0.5890545609274795 1105 68-69 0.537943915224183 1105 70-71 0.7350766317798616 1105 72-73 0.4275978942087306 1105 74-75 0.7319062586666405 1105 76-77 0.3960515781010159 1105 78-79 0.5700319252614641 1105 80-81 0.9317223762370084 1105 82-83 0.8373660994655054 1105 84-85 0.9308857900262772 1105 86-87 0.8917007805039994 1105 88-89 0.5030105426309994 1105 90-91 0.8558516634577238 1105 92-93 0.7106657399922263 1105 94-95 0.6371993997124505 1105 96-97 0.8866206347875298 1105 98-99 1.0242772503629354 1105 100-101 1.5027825568524449 1106 1 0.17836464476079072 1106 2 0.22610030511371804 1106 3 -0.04151495742882361 1106 4 0.059853629090952154 1106 5 0.028000731090322972 1106 6 0.05996731473594252 1106 7 -0.0625811462353596 1106 8 0.06119884233704198 1106 9 0.15650006704485975 1106 10-11 -0.021607804471798886 1106 12-13 0.11067522849187128 1106 14-15 0.23950817501995658 1106 16-17 0.19453884608397942 1106 18-19 0.17066372460060109 1106 20-21 0.027000304196782565 1106 22-23 -0.13299574070639153 1106 24-25 -0.24445360806316074 1106 26-27 -0.0018657971324174127 1106 28-29 -0.3044824008283413 1106 30-31 -0.2797329037999816 1106 32-33 0.1342190193637336 1106 34-35 0.16600760743004628 1106 36-37 -0.16797515530367946 1106 38-39 0.20931930551778066 1106 40-41 0.09884649564206427 1106 42-43 0.2859127221051381 1106 44-45 0.33821160661425864 1106 46-47 0.24917825212388323 1106 48-49 -0.07566113599749968 1106 50-51 0.18973881670677173 1106 52-53 -0.07824456164941296 1106 54-55 -0.13992294939104255 1106 56-57 -0.038749801439159626 1106 58-59 -0.416371283535824 1106 60-61 -0.4239133772314929 1106 62-63 -0.18046832951999647 1106 64-65 -0.1535747508327887 1106 66-67 -0.1147966569790384 1106 68-69 -0.35808417343063326 1106 70-71 0.15152385583076722 1106 72-73 -0.02094281239649476 1106 74-75 0.12339188517530886 1106 76-77 -0.004617721877039571 1106 78-79 -0.29079756782609323 1106 80-81 0.21080949495546264 1106 82-83 0.04186467308850439 1106 84-85 0.22798914749829535 1106 86-87 0.180015461214996 1106 88-89 -0.14116325592067724 1106 90-91 0.1559943011579641 1106 92-93 0.3591845023358715 1106 94-95 0.20100234024350172 1106 96-97 0.23487606599227107 1106 98-99 0.31161541143381655 1106 100-101 1.0408119621629588 1107 1 -0.01144367688484138 1107 2 -0.04619983513873649 1107 3 -0.08094927252933815 1107 4 -0.0470654919936635 1107 5 -0.12662759990546846 1107 6 -0.07572693491342619 1107 7 0.06939641337193336 1107 8 0.05937556042960779 1107 9 -0.0570342948064706 1107 10-11 -0.054333377169321295 1107 12-13 -0.19904894168110587 1107 14-15 -0.0560388095522697 1107 16-17 0.06783244122190268 1107 18-19 -0.1927537602053775 1107 20-21 -0.19950740968820924 1107 22-23 -0.2694146280369196 1107 24-25 -0.06621611390701077 1107 26-27 0.04008137444308346 1107 28-29 0.043051493514809636 1107 30-31 -0.1451606737859592 1107 32-33 -0.1110591480041947 1107 34-35 0.12020349335805491 1107 36-37 0.12539791622506868 1107 38-39 -0.017784481298484422 1107 40-41 0.02890727170564844 1107 42-43 -0.20119573979294358 1107 44-45 0.010090989503460435 1107 46-47 -0.08780164502431376 1107 48-49 0.03103591870095812 1107 50-51 -0.03752859797298669 1107 52-53 0.30179283900509546 1107 54-55 0.28313689165617717 1107 56-57 0.3155863369432623 1107 58-59 -0.11978409326000161 1107 60-61 -0.12876377461345356 1107 62-63 0.3470562146595313 1107 64-65 0.09254493126164931 1107 66-67 0.3848176487713175 1107 68-69 0.36797940768203574 1107 70-71 0.25546261225900935 1107 72-73 0.5226289501093468 1107 74-75 0.5111431708228089 1107 76-77 0.17393534030154711 1107 78-79 0.5316778879943804 1107 80-81 0.6024410985085247 1107 82-83 0.21782306486036163 1107 84-85 0.37802421061190117 1107 86-87 0.11339554536646901 1107 88-89 -0.04253071735125147 1107 90-91 -0.09310574559285456 1107 92-93 0.3534341517047359 1107 94-95 0.4417690536609875 1107 96-97 1.1503739622054567 1107 98-99 1.1541352805315235 1107 100-101 0.8473921398160655 1108 1 0.02660594237332603 1108 2 0.23647027048641434 1108 3 0.07457762503882748 1108 4 0.14161050898889727 1108 5 0.05324221095181514 1108 6 0.19186088134793522 1108 7 -0.11525109338316497 1108 8 -0.03942080409999704 1108 9 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0.20133111395109893 2316 64-65 0.3251397360956503 2316 66-67 0.10023992964365647 2316 68-69 0.2351206773028096 2316 70-71 0.056250916190109024 2316 72-73 0.22764353546842742 2316 74-75 0.28573686439136026 2316 76-77 0.39501812957783855 2316 78-79 -0.09400276209590785 2316 80-81 0.027027846667991184 2316 82-83 -0.3469463475580312 2316 84-85 -0.03338362380954507 2316 86-87 0.21032567455107554 2316 88-89 0.09167194968583559 2316 90-91 -0.2625841453705995 2316 92-93 -0.33446694566989876 2316 94-95 -0.24921580534720889 2316 96-97 -0.8126556252372445 2316 98-99 -1.517145452903673 2316 100-101 -1.9422001646706697 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 11.0 6 16.0 7 52.0 8 119.0 9 242.0 10 404.0 11 589.0 12 917.0 13 1264.0 14 1698.0 15 2344.0 16 3061.0 17 4119.0 18 3603.0 19 2225.0 20 1935.0 21 2035.0 22 2195.0 23 2471.0 24 2822.0 25 3449.0 26 4035.0 27 5080.0 28 6283.0 29 7785.0 30 9761.0 31 12077.0 32 15222.0 33 20011.0 34 26789.0 35 37185.0 36 54353.0 37 74826.0 38 49370.0 39 3003.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.89060493335105 19.707109959700635 14.715634825738451 21.686650281209864 2 15.989460661531691 20.796926764328987 39.991918364409806 23.221694209729517 3 20.182551851257326 24.83574208047116 30.04577634353845 24.935929724733064 4 12.939701621406217 17.451356429862212 37.27048066572022 32.33846128301135 5 13.506407967964313 38.157228370513664 33.93109670206201 14.40526695946002 6 31.75482146266947 36.88719894396892 17.23134995419991 14.126629639161706 7 28.986614197197753 33.37235334977156 20.09475499027267 17.546277462758024 8 26.970580014556404 35.13655622064851 19.024887021831837 18.86797674296325 9 26.904993178415566 16.97287693750986 19.990701613104083 36.13142827097049 10-11 25.06911524188259 27.318992785448025 26.970303276851165 20.641588695818218 12-13 26.282485906976362 24.237648367644578 27.89062651342608 21.589239211952986 14-15 22.898032942947598 26.02932874316456 26.323640107153135 24.748998206734708 16-17 22.042880566759603 29.387094319258892 27.040770438837423 21.529254675144077 18-19 21.795772991312965 27.398344509233606 30.06118839994133 20.7446940995121 20-21 22.557028722606425 26.661463997808237 30.12912581326293 20.652381466322407 22-23 22.133681285837632 26.4282472492196 30.003569926276874 21.4345015386659 24-25 22.177621328728417 26.66810570273389 30.153755469028898 21.000517499508792 26-27 22.067846142350707 26.822002119846022 29.964272569026445 21.145879168776826 28-29 21.55068515138642 27.273909728554685 30.15233427472751 21.023070845331382 30-31 21.882688289584745 26.82270406398317 30.00138371916036 21.293223927271722 32-33 21.868167451746643 27.121376500738954 29.7219654491008 21.288490598413603 34-35 22.161153914275594 27.044727461919944 29.788650150549064 21.0054684732554 36-37 22.396153313961534 26.67220146672201 29.718001937180023 21.21364328213643 38-39 22.075226547225185 27.002741121106077 29.825224125740107 21.096808205928625 40-41 22.002507250547943 27.22982023068919 29.610047820407804 21.157624698355065 42-43 22.17669735789739 27.160355453712697 29.32659177895672 21.336355409433196 44-45 22.360947179507757 27.24268811237967 29.05327711766961 21.34308759044297 46-47 22.461170187005763 27.554811437585187 28.401629987339234 21.58238838806982 48-49 22.817841932082654 27.721661210291376 28.042955464354602 21.41754139327136 50-51 22.643370886639932 28.008899884600375 27.88907245823336 21.45865677052633 52-53 22.83604352542673 28.197713033275036 27.550698487900995 21.41554495339724 54-55 22.207616277541238 28.060479680747875 27.990048278028308 21.74185576368258 56-57 22.546878675910932 28.18560495979851 27.719378364539654 21.548137999750903 58-59 22.500346020761246 27.866989619377165 27.841245674740485 21.79141868512111 60-61 22.463988750982626 27.7694892547526 28.3874378591437 21.379084135121072 62-63 22.394406933763626 27.85725060163934 28.38935025719303 21.358992207404004 64-65 22.110812172343735 27.92766235419815 28.189925763539435 21.771599709918675 66-67 22.029517333753983 28.0452030873371 27.94516074576515 21.980118833143763 68-69 22.158864486705486 27.9796431180677 28.06404835174567 21.79744404348115 70-71 22.33342465677606 28.103820917496186 27.866518335256664 21.696236090471093 72-73 22.22591205829203 27.779761064665298 27.952168655027076 22.042158222015594 74-75 22.363283141782595 27.989007856670423 27.65857937893837 21.989129622608612 76-77 22.103745440657985 27.92558932435948 27.867058165857657 22.103607069124884 78-79 22.277344182442157 27.7472877227942 27.66218310638769 22.313184988375955 80-81 22.28585188711624 28.201288708302425 27.516108853780324 21.996750550801014 82-83 22.146304197696256 28.432122873714818 27.389168833721072 22.032404094867854 84-85 22.02268404835794 28.272286094739265 27.233315708025234 22.471714148877563 86-87 21.92910105922176 27.98883485606264 27.23448186148468 22.847582223230916 88-89 21.881982263838555 27.90953616310845 27.37961088977056 22.828870683282435 90-91 22.03123269465057 27.516059364270685 27.858151511795327 22.59455642928342 92-93 21.99538795442214 27.47586012158526 27.583963590862282 22.944788333130322 94-95 21.47373520658869 27.840127344452902 27.721918471866562 22.96421897709184 96-97 21.532152439919255 27.921382071723077 27.93038137669983 22.616084111657837 98-99 21.550087712861185 28.322046734963163 27.184479461898558 22.943386090277095 100-101 21.027940325291965 29.090173945072017 26.664469621231213 23.217416108404805 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 58.0 1 45.5 2 37.5 3 44.0 4 48.5 5 53.5 6 68.5 7 130.5 8 202.0 9 254.5 10 288.5 11 304.0 12 330.0 13 362.5 14 416.0 15 442.5 16 470.5 17 560.0 18 608.0 19 637.5 20 688.5 21 768.0 22 885.5 23 1007.0 24 1171.5 25 1442.5 26 1803.0 27 2318.5 28 2941.5 29 3675.5 30 4617.0 31 5465.0 32 6230.0 33 6980.5 34 7838.0 35 8769.5 36 9821.5 37 10606.0 38 11025.0 39 11286.0 40 11520.5 41 12118.5 42 12700.5 43 13240.5 44 13590.5 45 13613.0 46 13637.5 47 14074.0 48 15164.0 49 14836.5 50 13567.5 51 12806.0 52 12067.5 53 11547.0 54 10834.0 55 9824.5 56 8808.5 57 7757.0 58 6913.0 59 6092.5 60 5057.5 61 4136.5 62 3234.0 63 2465.0 64 1787.0 65 1284.5 66 975.0 67 833.0 68 627.0 69 367.0 70 269.5 71 206.0 72 159.5 73 134.0 74 100.0 75 90.5 76 72.5 77 45.5 78 28.5 79 20.0 80 18.0 81 14.5 82 16.5 83 14.5 84 7.0 85 2.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015774049198429237 2 0.011069508209424025 3 0.008578868862303619 4 0.0 5 0.0016604262314136038 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0011069508209424026 14-15 2.7673770523560065E-4 16-17 1.3836885261780032E-4 18-19 0.0027673770523560064 20-21 0.0 22-23 2.7673770523560065E-4 24-25 0.0 26-27 0.0016604262314136038 28-29 0.002629008199738206 30-31 0.002213901641884805 32-33 0.008578868862303619 34-35 0.002490639347120406 36-37 8.302131157068019E-4 38-39 0.001245319673560203 40-41 2.7673770523560065E-4 42-43 0.0033208524628272076 44-45 0.0017987950840314042 46-47 1.3836885261780032E-4 48-49 5.534754104712013E-4 50-51 0.0 52-53 2.7673770523560065E-4 54-55 0.0023522704945026056 56-57 0.01328340985130883 58-59 0.02850398363926687 60-61 0.02130880330314125 62-63 0.013421778703926631 64-65 0.020755327892670046 66-67 0.0016604262314136038 68-69 2.7673770523560065E-4 70-71 0.0 72-73 0.0 74-75 0.0011069508209424026 76-77 0.0019371639366492046 78-79 0.00913234427277482 80-81 0.01688100001937164 82-83 0.01978674592434545 84-85 0.035975901680628086 86-87 0.013836885261780032 88-89 0.04704540989005211 90-91 0.053410377110470926 92-93 0.03459221315445008 94-95 0.03542242627015688 96-97 0.059221868920418544 98-99 0.06406477876204154 100-101 0.07250527877172737 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 361353.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.55758630842891 #Duplication Level Percentage of deduplicated Percentage of total 1 88.26606641448038 65.80904864803139 2 7.464360722227811 11.130494375694935 3 1.8250058412439283 4.082040915657933 4 0.8065464327045201 2.4053662107249085 5 0.41363058389669094 1.5419648979341691 6 0.24329956825183602 1.0883897135243854 7 0.18650173561995148 0.9733583475109424 8 0.11861136603307001 0.7074701728137016 9 0.10255531659347641 0.6881649181475732 >10 0.5210452376970759 6.926925954059055 >50 0.03640372030336033 1.8633632073309627 >100 0.014858661346897582 1.8647479896012225 >500 3.7146653367243956E-4 0.19331562405193126 >1k 7.429330673448791E-4 0.7253490249169169 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1365 0.3777469676465949 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1254 0.3470290823654432 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 698 0.19316291825444926 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 451 0.1248087050612559 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 399 0.11041834438900466 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 2.7673770523560065E-4 0.0 8 0.0 0.0 0.0 2.7673770523560065E-4 0.0 9 0.0 0.0 0.0 2.7673770523560065E-4 0.0 10-11 0.0 0.0 0.0 2.7673770523560065E-4 0.0 12-13 0.0 0.0 0.0 2.7673770523560065E-4 0.0 14-15 0.0 0.0 0.0 2.7673770523560065E-4 0.0 16-17 0.0 0.0 0.0 2.7673770523560065E-4 0.0 18-19 0.0 0.0 0.0 2.7673770523560065E-4 0.0 20-21 0.0 0.0 0.0 4.15106557853401E-4 0.0 22-23 0.0 0.0 0.0 6.918442630890016E-4 0.0 24-25 0.0 0.0 0.0 0.0016604262314136038 0.0 26-27 0.0 0.0 0.0 0.0024906393471204056 0.0 28-29 0.0 0.0 0.0 0.004981278694240811 0.0 30-31 0.0 0.0 0.0 0.007748655746596818 0.0 32-33 0.0 0.0 0.0 0.014113622967015634 0.0 34-35 0.0 0.0 0.0 0.020755327892670046 0.0 36-37 0.0 0.0 0.0 0.025044762323821856 0.0 38-39 0.0 0.0 0.0 0.03265504921780087 0.0 40-41 0.0 0.0 0.0 0.0416490246379579 0.0 42-43 0.0 0.0 0.0 0.06600194269869075 0.0 44-45 0.0 0.0 0.0 0.09187691813821941 0.0 46-47 0.0 0.0 0.0 0.12051927063010409 0.0 48-49 2.7673770523560065E-4 0.0 0.0 0.15400453296361177 0.0 50-51 2.7673770523560065E-4 0.0 0.0 0.19274781169659586 0.0 52-53 2.7673770523560065E-4 0.0 0.0 0.2620706068581138 0.0 54-55 2.7673770523560065E-4 0.0 0.0 0.31395892658978897 0.0 56-57 2.7673770523560065E-4 0.0 0.0 0.4188425168740816 0.0 58-59 2.7673770523560065E-4 2.7673770523560065E-4 0.0 0.5619159104808871 0.0 60-61 2.7673770523560065E-4 2.7673770523560065E-4 0.0 0.7023602958879545 0.0 62-63 2.7673770523560065E-4 2.7673770523560065E-4 0.0 0.9097752059620372 0.0 64-65 2.7673770523560065E-4 2.7673770523560065E-4 0.0 1.0383198700439737 0.0 66-67 2.7673770523560065E-4 2.7673770523560065E-4 0.0 1.2872454359033965 0.0 68-69 2.7673770523560065E-4 2.7673770523560065E-4 0.0 1.5692411575384735 0.0 70-71 2.7673770523560065E-4 2.7673770523560065E-4 0.0 1.770429469244755 0.0 72-73 2.7673770523560065E-4 2.7673770523560065E-4 0.0 2.1366918221240727 0.0 74-75 2.7673770523560065E-4 2.7673770523560065E-4 0.0 2.4768024618586257 0.0 76-77 2.7673770523560065E-4 2.7673770523560065E-4 0.0 2.697915888341871 0.0 78-79 2.7673770523560065E-4 2.7673770523560065E-4 0.0 2.9341115197604557 0.0 80-81 2.7673770523560065E-4 2.7673770523560065E-4 0.0 3.388376462904694 0.0 82-83 2.7673770523560065E-4 2.7673770523560065E-4 0.0 3.693894889484797 0.0 84-85 2.7673770523560065E-4 2.7673770523560065E-4 0.0 4.163242037564376 0.0 86-87 2.7673770523560065E-4 2.7673770523560065E-4 0.0 5.043406309066204 0.0 88-89 2.7673770523560065E-4 2.7673770523560065E-4 0.0 6.200031548098397 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACACG 55 0.0025757668 34.549633 1 TAAGGTG 125 5.638867E-9 34.19467 5 GTAAGGT 135 1.1941665E-8 31.66173 4 GGCGCAT 40 4.4815542E-4 29.699312 88-89 GGTATCA 1620 0.0 29.61778 1 GTACGTT 35 0.008373281 27.153656 94-95 ATGGGTA 175 4.945832E-9 27.138628 5 CCTATAC 70 0.008390469 27.138626 3 TCGCGGT 45 8.9524616E-4 26.384777 26-27 GTACATG 1770 0.0 26.302614 1 GTATCAA 2270 0.0 26.159552 1 TACATGG 1795 0.0 25.138834 2 GGTAAGG 175 1.4825127E-7 24.424765 3 ACATGGG 1795 0.0 23.812445 3 TGCGTAA 60 1.7069558E-4 23.75616 60-61 CATGGGT 285 3.6379788E-12 23.329699 4 GGGTAGG 185 2.5286863E-7 23.110903 1 GTTGTAG 165 2.4196797E-6 23.033089 1 ACTATAC 105 0.0021953175 22.61552 3 CTATACC 105 0.0021953175 22.61552 4 >>END_MODULE