FastQCFastQC Report
Thu 26 May 2016
SRR936539_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936539_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002836
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46940.46807254625881No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36920.36815590983969465No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36170.36067711968856325No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30910.30822587142862845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30170.3008467984795121No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA27160.27083192067297146No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24160.2409167600684459No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23240.23174277748305802No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT21530.21469113593847847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17560.17510340673848962No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15230.15186929866897478No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13820.13780917318484778No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA12590.12554395733699228No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG12530.12494565412490177No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC11810.11776601557981564No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC11590.11557223713548376No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT11030.10998807382263899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG9150.028.5564561
TACCTGG9150.028.555032
GTACATG55500.024.823771
TACATGG56200.024.2597682
ACATGGG55950.023.7726353
GCCGATC1000.001651007423.7561538
ACCGTGC1201.707602E-423.7561518
GAGTACT35750.022.59213412-13
TAGCCCT7000.021.0380124
GACCGCG1150.003718443420.6564927
TATCACG1150.003720594320.654432
ATGGGCG1150.003720594320.654435
CATGGGG19300.020.4296934
AGTACTT38150.020.36139912-13
GTACACG1656.313948E-520.1547221
GTATAGG2601.4868419E-820.0993521
GTACTTT40300.019.92799614-15
GTATAGC4350.019.65831
GTGTAGC6300.019.6062951
AGGACCG1507.714446E-419.0020755
Produced by FastQC (version 0.11.5)