##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936539_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1002836 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.670318975385804 31.0 31.0 34.0 30.0 34.0 2 31.759535956028703 33.0 31.0 34.0 30.0 34.0 3 31.78086945422781 33.0 31.0 34.0 30.0 34.0 4 35.38909652226286 37.0 35.0 37.0 33.0 37.0 5 35.403913501310285 37.0 35.0 37.0 33.0 37.0 6 35.40522378534476 37.0 35.0 37.0 33.0 37.0 7 35.34071573018918 37.0 35.0 37.0 33.0 37.0 8 35.30699436398374 37.0 35.0 37.0 33.0 37.0 9 36.858909133696834 39.0 37.0 39.0 33.0 39.0 10-11 36.739955486241016 39.0 37.0 39.0 32.5 39.0 12-13 36.66504991843133 39.0 37.0 39.0 32.0 39.0 14-15 37.785233078987986 40.0 37.0 41.0 32.5 41.0 16-17 37.70417246688392 40.0 37.0 41.0 32.0 41.0 18-19 37.700416618470015 40.0 37.0 41.0 32.0 41.0 20-21 37.572731732805764 40.0 37.0 41.0 32.0 41.0 22-23 37.68469520440032 40.0 37.0 41.0 32.0 41.0 24-25 37.729623288354226 40.0 37.0 41.0 32.0 41.0 26-27 37.63493033756268 40.0 37.0 41.0 32.0 41.0 28-29 37.502983040098286 40.0 37.0 41.0 32.0 41.0 30-31 37.32177444766642 39.5 37.0 41.0 31.5 41.0 32-33 37.13305266264872 39.0 36.0 41.0 31.0 41.0 34-35 36.93727139831438 39.0 36.0 41.0 31.0 41.0 36-37 36.73137232807757 39.0 36.0 41.0 30.0 41.0 38-39 36.522702116796765 39.0 35.0 40.5 30.0 41.0 40-41 36.33131788248527 39.0 35.0 40.0 30.0 41.0 42-43 36.113248327742525 39.0 35.0 40.0 30.0 41.0 44-45 35.844837540734474 38.5 35.0 40.0 29.0 41.0 46-47 35.63763616383935 39.0 35.0 40.0 27.5 41.0 48-49 35.4742824350143 39.0 35.0 40.0 27.0 41.0 50-51 34.5316951126605 38.0 34.0 39.5 25.0 40.5 52-53 34.65086165634261 38.0 34.0 39.5 26.0 40.5 54-55 35.10123539641576 38.5 35.0 40.0 26.0 41.0 56-57 34.99468906182068 38.0 34.5 40.0 26.0 41.0 58-59 34.777711909026 38.0 34.0 40.0 26.0 41.0 60-61 34.474728170907305 38.0 34.0 40.0 24.5 41.0 62-63 34.133406160129866 38.0 34.0 40.0 23.0 41.0 64-65 33.77584370724625 37.0 33.0 40.0 22.5 41.0 66-67 33.30144859179367 36.5 33.0 39.0 22.0 41.0 68-69 32.91949630846918 36.0 32.0 39.0 20.5 41.0 70-71 32.492613946846745 35.5 32.0 39.0 19.5 40.0 72-73 32.088461622837634 35.0 31.5 38.0 18.5 40.0 74-75 31.643349460928803 35.0 31.0 37.0 17.0 39.5 76-77 31.154837879772963 35.0 31.0 37.0 12.5 39.0 78-79 30.64832086203527 34.5 30.5 36.0 7.5 39.0 80-81 30.154429537830712 34.0 30.0 36.0 7.0 37.5 82-83 29.71604080826775 34.0 29.5 35.5 2.0 37.0 84-85 29.33568948462161 34.0 29.0 35.0 2.0 37.0 86-87 28.97168480190181 34.0 29.0 35.0 2.0 36.0 88-89 28.525255874340374 34.0 29.0 35.0 2.0 36.0 90-91 28.084296933895473 34.0 27.5 35.0 2.0 35.5 92-93 27.622436270736195 34.0 26.5 35.0 2.0 35.0 94-95 27.133655951720918 33.0 26.0 35.0 2.0 35.0 96-97 26.556292853467568 33.0 25.0 35.0 2.0 35.0 98-99 25.80433889489408 33.0 24.0 35.0 2.0 35.0 100-101 22.952897083870145 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3553412848975661 1101 2 0.4403258085135491 1101 3 0.4843940281356822 1101 4 0.528923881685337 1101 5 0.47785006665243657 1101 6 0.4676295443232803 1101 7 0.525028029994985 1101 8 0.5625124454169494 1101 9 0.5506501198855247 1101 10-11 0.7133790032762377 1101 12-13 0.6655107992076097 1101 14-15 0.7847927302666164 1101 16-17 0.845953687678417 1101 18-19 0.7819372272500331 1101 20-21 0.7706526717623774 1101 22-23 0.7154260466520483 1101 24-25 0.6523384272259065 1101 26-27 0.6667534584928134 1101 28-29 0.7623719202041457 1101 30-31 0.7263465079767997 1101 32-33 0.5929716903734104 1101 34-35 0.5932164886567293 1101 36-37 0.5258465291132168 1101 38-39 0.586477559779091 1101 40-41 0.6076779986426217 1101 42-43 0.7157391280718386 1101 44-45 0.6357093849351614 1101 46-47 0.7827249393928142 1101 48-49 0.8272246252465223 1101 50-51 0.7488469924135117 1101 52-53 0.6172755828560597 1101 54-55 0.7366729429707064 1101 56-57 0.6837184795579958 1101 58-59 0.8507104664957268 1101 60-61 0.7701659800482119 1101 62-63 0.7536121877315196 1101 64-65 0.6680487063330602 1101 66-67 0.8823073095145801 1101 68-69 0.7836025493561394 1101 70-71 0.7874333102563114 1101 72-73 0.6648646340591782 1101 74-75 0.548935428794632 1101 76-77 0.6427555871781685 1101 78-79 0.5963605528696689 1101 80-81 0.6561876444501102 1101 82-83 0.4500153195046721 1101 84-85 0.6978432931139444 1101 86-87 0.6796547987623072 1101 88-89 0.6650226640252477 1101 90-91 0.757006657377314 1101 92-93 0.6819310623969415 1101 94-95 0.630337926259589 1101 96-97 0.6483085296868971 1101 98-99 0.5879665505306271 1101 100-101 0.7800553758293916 1102 1 0.07384392913875715 1102 2 0.15547113340842955 1102 3 0.1743963039170353 1102 4 0.07045960061550005 1102 5 0.12972704441479976 1102 6 0.256295611339624 1102 7 0.25787676639448165 1102 8 0.22035305402590666 1102 9 0.40226267352893785 1102 10-11 0.3814088810342682 1102 12-13 0.3227672906029895 1102 14-15 0.4985086477316045 1102 16-17 0.2585934856757319 1102 18-19 0.034056684096881895 1102 20-21 0.20728841670148057 1102 22-23 0.1401507854790438 1102 24-25 -0.00269673901450318 1102 26-27 -0.11018447769860984 1102 28-29 -0.14978291962419377 1102 30-31 0.1526055027316673 1102 32-33 0.02026562379055008 1102 34-35 0.14184587224509215 1102 36-37 -0.21056813992314716 1102 38-39 -0.40846478709332956 1102 40-41 -0.22328834146748022 1102 42-43 -0.07526124038799509 1102 44-45 -0.0015378407790009874 1102 46-47 -0.039683108203526274 1102 48-49 0.2186787953449283 1102 50-51 0.3594448185004637 1102 52-53 0.29807123936479485 1102 54-55 0.45444164772600715 1102 56-57 0.5375065718030001 1102 58-59 0.2369758551124832 1102 60-61 0.15434117186406837 1102 62-63 0.09373096184397411 1102 64-65 0.13489355223014599 1102 66-67 0.16989536057878496 1102 68-69 0.03582414543745216 1102 70-71 0.2134511805584438 1102 72-73 -0.03997465242918352 1102 74-75 -0.07303209255436371 1102 76-77 0.1091366071616946 1102 78-79 0.06435683576012607 1102 80-81 0.2596652550964329 1102 82-83 0.0938875072891463 1102 84-85 -0.31057113750723886 1102 86-87 0.043067549640106506 1102 88-89 0.1217519327408425 1102 90-91 0.019295605749586997 1102 92-93 0.03950562132883917 1102 94-95 0.019189306900276648 1102 96-97 0.06154734584711363 1102 98-99 0.04978137778798697 1102 100-101 0.12237651025221297 1103 1 0.25952095249312634 1103 2 0.13471073449553828 1103 3 0.27602869140609343 1103 4 0.3072882200820857 1103 5 0.440466829757284 1103 6 0.2768521777406576 1103 7 0.4483006689233875 1103 8 0.38108247500338166 1103 9 0.2754097553843948 1103 10-11 0.4581843543402684 1103 12-13 0.3435305685061607 1103 14-15 0.6586398145287902 1103 16-17 0.704943501798077 1103 18-19 0.578179225439186 1103 20-21 0.5717570702553019 1103 22-23 0.5116798747968332 1103 24-25 0.48484327895040025 1103 26-27 0.3879352537491769 1103 28-29 0.41049162088895486 1103 30-31 0.3456420889401457 1103 32-33 0.30921289220457027 1103 34-35 0.4964070565465377 1103 36-37 0.6056993067579484 1103 38-39 0.7115695304708183 1103 40-41 0.320060615186442 1103 42-43 0.15017924512491732 1103 44-45 0.3301751008581064 1103 46-47 0.6562555002077346 1103 48-49 0.4866572259497488 1103 50-51 0.5531948185004651 1103 52-53 0.7214083345787614 1103 54-55 0.8864419125931065 1103 56-57 0.7563772014957522 1103 58-59 0.5709729876381644 1103 60-61 0.8147337509787107 1103 62-63 0.686287044574641 1103 64-65 0.7088424328787326 1103 66-67 0.6588460837811354 1103 68-69 0.6373718906122718 1103 70-71 0.6366256128215682 1103 72-73 0.539183185333961 1103 74-75 0.5278094363046328 1103 76-77 0.7341795041168773 1103 78-79 0.46950187929046194 1103 80-81 0.4776796730797592 1103 82-83 0.4726480443935728 1103 84-85 0.5459221371714662 1103 86-87 0.529613740160169 1103 88-89 0.5336876506458559 1103 90-91 0.5616170507723872 1103 92-93 0.6139039123602075 1103 94-95 0.7138591442949043 1103 96-97 0.5259166742362602 1103 98-99 0.3822883449444632 1103 100-101 0.29831707637685767 1104 1 0.18137211240223294 1104 2 0.21202133334098505 1104 3 0.2068016370576622 1104 4 0.4591868974310387 1104 5 0.33857466208594644 1104 6 0.294362889848081 1104 7 0.22562800693984286 1104 8 0.429779994335199 1104 9 0.4823296388900218 1104 10-11 0.5871692871623182 1104 12-13 0.39983767649690094 1104 14-15 0.17671298843595196 1104 16-17 0.2369291073936992 1104 18-19 0.21941614114751928 1104 20-21 -0.10803894274352643 1104 22-23 0.03781542873192478 1104 24-25 0.16538902723302584 1104 26-27 0.06750166432576066 1104 28-29 0.06790268041434899 1104 30-31 0.10913010888862829 1104 32-33 -0.0972510573290748 1104 34-35 -0.02731061713198102 1104 36-37 -0.11809090332825889 1104 38-39 0.06887900054457674 1104 40-41 -0.19083844504699954 1104 42-43 -0.06274076536752915 1104 44-45 -0.0646054203906985 1104 46-47 -0.13398472934338201 1104 48-49 -0.00675389001423099 1104 50-51 -0.24369297838733672 1104 52-53 -0.1501185279426167 1104 54-55 0.00516881205610531 1104 56-57 -0.04536848239558111 1104 58-59 -0.137317455577886 1104 60-61 -0.25386991355290434 1104 62-63 -0.10138875680516435 1104 64-65 -0.2234671814982292 1104 66-67 -0.24935687962933883 1104 68-69 -0.17323785774218692 1104 70-71 -0.032458934101661896 1104 72-73 -0.16694687866611702 1104 74-75 -0.16602150576436614 1104 76-77 -0.12037120947847413 1104 78-79 0.008156216691862284 1104 80-81 0.1475283856066234 1104 82-83 -0.18787707815191368 1104 84-85 -0.2624807342697757 1104 86-87 0.04020014346387768 1104 88-89 -0.09600532986936017 1104 90-91 -0.3059100477648862 1104 92-93 -0.4862344904406868 1104 94-95 -0.38520981146943 1104 96-97 -0.2927647536855744 1104 98-99 -0.36842723858239523 1104 100-101 0.034432382969850295 1105 1 0.2213291881588333 1105 2 0.051632044073411265 1105 3 0.18598259714554288 1105 4 0.264575492795764 1105 5 0.19111737620384162 1105 6 0.38845025123854526 1105 7 0.28041701160951504 1105 8 0.24102669341006333 1105 9 0.507609203549066 1105 10-11 0.3984589886668175 1105 12-13 0.32504863273411644 1105 14-15 0.2126991807467249 1105 16-17 0.27806386332559185 1105 18-19 0.17314748151370196 1105 20-21 0.23645449840861943 1105 22-23 -0.2422945793017135 1105 24-25 0.11873530038837288 1105 26-27 -0.11193672394317389 1105 28-29 -0.19253176140434647 1105 30-31 -0.19773816098680896 1105 32-33 -0.08747777786605582 1105 34-35 0.03909393017948304 1105 36-37 -0.04100709354755594 1105 38-39 0.0045786403014957955 1105 40-41 -0.18059507084180382 1105 42-43 -0.23784079488283538 1105 44-45 -0.11286587437907514 1105 46-47 -0.10391137110773485 1105 48-49 -0.0898443573529093 1105 50-51 -0.21768823639213508 1105 52-53 -0.046131811784071886 1105 54-55 -0.21772744067308025 1105 56-57 -0.1928873399383093 1105 58-59 -0.1397676167392632 1105 60-61 -0.012338948871580158 1105 62-63 0.03545963421984766 1105 64-65 -0.014236905442182035 1105 66-67 -0.0439655477463603 1105 68-69 0.17895204098708462 1105 70-71 -0.08605657600480043 1105 72-73 -0.219876028253978 1105 74-75 0.07600594151367801 1105 76-77 -0.22314701631156098 1105 78-79 0.10193083313950879 1105 80-81 0.26532181366257745 1105 82-83 0.10405468872018986 1105 84-85 -0.06260153200394214 1105 86-87 -0.2040986139613743 1105 88-89 -0.5043436964416195 1105 90-91 -0.4766971290531643 1105 92-93 -0.5624709568919499 1105 94-95 -0.30954214988291184 1105 96-97 -0.2681536813418042 1105 98-99 -0.19559656455840368 1105 100-101 0.10286781775372944 1106 1 0.2147077187011348 1106 2 0.20862361642491578 1106 3 0.21649106930752282 1106 4 0.41604454453040063 1106 5 0.27891078236834943 1106 6 0.4543188089803607 1106 7 0.5420893368299957 1106 8 0.6564621171677274 1106 9 0.7597406871157517 1106 10-11 0.5771653058064956 1106 12-13 0.5697128906872493 1106 14-15 0.5217740908829072 1106 16-17 0.5959894780923918 1106 18-19 0.3767475182640325 1106 20-21 0.41213069380927436 1106 22-23 0.419945666726079 1106 24-25 0.4825318801860874 1106 26-27 0.31612450381700086 1106 28-29 0.4095935614964574 1106 30-31 0.4882277708869154 1106 32-33 0.5194891874326615 1106 34-35 0.6407751865849818 1106 36-37 0.4053595023960881 1106 38-39 0.32929523186501086 1106 40-41 0.4744083513546755 1106 42-43 0.4667150353505818 1106 44-45 0.48888589138172023 1106 46-47 0.5007156094751295 1106 48-49 0.5262275735849613 1106 50-51 0.15301576747092582 1106 52-53 0.26537994440455037 1106 54-55 0.539953983815316 1106 56-57 0.5345291901610025 1106 58-59 0.46872811958138527 1106 60-61 0.3339431782888411 1106 62-63 0.495400349384127 1106 64-65 0.3598846655062573 1106 66-67 0.6203735979793805 1106 68-69 0.5976494126974501 1106 70-71 0.4935709209344594 1106 72-73 0.09370471457276608 1106 74-75 0.35875308570673425 1106 76-77 0.2875586541362587 1106 78-79 0.3640458004238809 1106 80-81 0.5390309404932445 1106 82-83 0.19863301784788945 1106 84-85 0.46820144135420705 1106 86-87 0.5151003712736859 1106 88-89 0.3564779583346045 1106 90-91 0.3966328141333868 1106 92-93 0.7552417053775002 1106 94-95 0.6065866158686255 1106 96-97 0.3485026225862775 1106 98-99 0.5678652026339464 1106 100-101 0.5405393281994435 1107 1 0.06933747227770581 1107 2 0.23706208871881884 1107 3 0.268139630979924 1107 4 0.28728874800479787 1107 5 0.23165647651607202 1107 6 0.33447764906396316 1107 7 0.4325888768220594 1107 8 0.4145304397598011 1107 9 0.6109743518044013 1107 10-11 0.650104226501746 1107 12-13 0.3812222196792021 1107 14-15 0.08752118014302113 1107 16-17 0.1565197910293321 1107 18-19 0.19723764144092115 1107 20-21 0.3303119562815269 1107 22-23 0.2005516701198431 1107 24-25 0.10352402716578268 1107 26-27 0.2124517137701929 1107 28-29 0.18706694564603055 1107 30-31 0.24523254274386375 1107 32-33 0.08938485964392129 1107 34-35 0.07241777473124245 1107 36-37 0.19432534729023843 1107 38-39 0.12675942359353343 1107 40-41 -0.014377746114384138 1107 42-43 0.20337964120231788 1107 44-45 0.37562450860327345 1107 46-47 0.2758073208981031 1107 48-49 0.2891853665991846 1107 50-51 0.25889922645206553 1107 52-53 0.49082252311078634 1107 54-55 0.5384339071189572 1107 56-57 0.19513850530241683 1107 58-59 0.36189292003697915 1107 60-61 0.2678173272354556 1107 62-63 0.3833135537398462 1107 64-65 0.37049812033223617 1107 66-67 0.39254934654654505 1107 68-69 0.5501614274548459 1107 70-71 0.38384335860512664 1107 72-73 0.30165112925773485 1107 74-75 0.5766409335272904 1107 76-77 0.6800251246257254 1107 78-79 0.5967958896012 1107 80-81 0.4166572221511906 1107 82-83 0.6317395841613802 1107 84-85 0.38344546221821574 1107 86-87 0.4996581871915815 1107 88-89 0.29442021785955674 1107 90-91 0.15369523206511104 1107 92-93 0.28052511901555377 1107 94-95 0.32838588527233625 1107 96-97 0.3095133710644582 1107 98-99 0.3953324352896388 1107 100-101 0.43999528853275294 1108 1 0.06736859707044474 1108 2 0.10791420741222524 1108 3 0.03590009898344704 1108 4 0.12442070118476067 1108 5 -0.011544302833776499 1108 6 0.17050339122577185 1108 7 0.10178094096753654 1108 8 -0.017170665139175867 1108 9 0.14220574753273496 1108 10-11 0.19859341613862824 1108 12-13 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2316 80-81 -3.381433013004578 2316 82-83 -3.012102612494175 2316 84-85 -2.790498847891005 2316 86-87 -2.7651899654281777 2316 88-89 -3.168292509160434 2316 90-91 -3.347749198553455 2316 92-93 -3.92317939431166 2316 94-95 -3.6941308655266702 2316 96-97 -4.18926472022445 2316 98-99 -5.895796843773013 2316 100-101 -6.904013491273691 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1906.0 3 721.0 4 690.0 5 735.0 6 849.0 7 1015.0 8 1290.0 9 1567.0 10 1990.0 11 2638.0 12 3508.0 13 4443.0 14 5150.0 15 6189.0 16 7995.0 17 10631.0 18 8250.0 19 5820.0 20 5893.0 21 6349.0 22 7212.0 23 8053.0 24 9066.0 25 10601.0 26 12030.0 27 14470.0 28 17001.0 29 20546.0 30 25261.0 31 31391.0 32 40007.0 33 52216.0 34 70082.0 35 97845.0 36 139369.0 37 189557.0 38 156453.0 39 24041.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.10877478523288 18.644299371505735 14.269073733886234 21.977852109375156 2 14.771045503683936 20.607500309155387 40.986026176485844 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46-47 21.78606080610115 27.15752567491701 28.81484044605899 22.241573072922847 48-49 21.887774765750777 27.01939458423474 28.848864440337184 22.2439662096773 50-51 21.6697247706422 27.388113282808135 29.2016354208217 21.74052652572796 52-53 21.74164729181739 27.69510676817542 28.596629349618347 21.966616590388846 54-55 21.19908175639021 27.056823802533746 29.038656380636308 22.705438060439736 56-57 21.880571743110483 27.56614294774378 28.25050756460729 22.302777744538446 58-59 22.271429746729723 27.11599973074919 28.28744019765004 22.325130324871047 60-61 21.791067101444938 27.03074361045462 29.330780506775962 21.84740878132448 62-63 21.954629376180097 27.10620299861542 29.045017124047508 21.894150501156975 64-65 21.556011156460684 27.74865455357704 28.823964651928996 21.87136963803328 66-67 21.50164657729339 27.42623313779189 28.392886706306857 22.67923357860786 68-69 21.240210628908955 27.69070849583486 28.71901331419311 22.35006756106308 70-71 21.733844818469336 27.38688629266395 28.19965878819926 22.679610100667457 72-73 21.627523354838967 26.981807809646718 28.687631085796806 22.70303774971751 74-75 21.316254355279053 27.978272730990632 28.3235529700684 22.381919943661917 76-77 21.649270654159558 27.25876778480246 28.371450956610296 22.720510604427695 78-79 21.616945789019717 27.153879884682752 28.734129286420888 22.49504503987664 80-81 21.74715105330442 27.76050185771583 28.216073857552164 22.27627323142758 82-83 21.63283833733221 27.492031345867023 28.499598449666543 22.375531867134228 84-85 21.471239881336178 27.42931459127156 28.277454721893513 22.82199080549875 86-87 21.668126970100598 27.517901391867817 28.347756011471652 22.466215626559936 88-89 21.802318188097082 27.08319515597128 28.127043050996047 22.987443604935585 90-91 21.487592175828386 26.878032624656832 28.607576374257803 23.026798825256975 92-93 21.581576369813142 27.351927323650333 28.663291381652574 22.403204924883948 94-95 21.219046498256944 27.34235337119469 28.51000829266371 22.928591837884657 96-97 20.666573247607218 27.268275435956994 28.82664497748717 23.238506338948618 98-99 21.072099143805207 27.54528893331817 28.000772191527446 23.38183973134918 100-101 21.18007931989848 27.20680500666321 28.35389507201806 23.259220601420257 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 95.0 1 84.5 2 122.0 3 169.0 4 142.5 5 136.5 6 231.5 7 464.0 8 775.5 9 943.0 10 940.5 11 885.5 12 865.5 13 982.0 14 1096.0 15 1088.5 16 1171.0 17 1325.5 18 1390.0 19 1534.5 20 1743.5 21 1970.0 22 2297.0 23 2772.0 24 3356.5 25 4091.0 26 5237.5 27 6744.0 28 8256.0 29 10043.5 30 12435.0 31 14966.0 32 17625.5 33 19905.0 34 22178.0 35 24361.5 36 26762.0 37 29303.5 38 30336.0 39 30953.5 40 31731.5 41 32720.0 42 33485.5 43 34518.5 44 35869.0 45 37120.5 46 38470.0 47 43056.0 48 56881.5 49 55849.5 50 40642.5 51 34011.5 52 30495.5 53 28536.5 54 26731.0 55 24312.5 56 21786.0 57 19194.5 58 17196.5 59 15068.0 60 12657.0 61 10467.5 62 8384.0 63 6240.5 64 4357.0 65 3304.0 66 2459.5 67 1843.5 68 1380.0 69 990.0 70 750.5 71 583.5 72 481.5 73 424.5 74 330.0 75 240.5 76 179.0 77 122.0 78 82.0 79 48.5 80 24.5 81 19.0 82 17.5 83 11.0 84 6.5 85 2.5 86 2.5 87 4.0 88 3.5 89 2.0 90 0.5 91 0.5 92 0.5 93 1.0 94 2.0 95 2.0 96 1.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012165498645840395 2 0.010370589009568863 3 0.013860691080096847 4 0.018447682372790764 5 0.028020533766238948 6 0.046567933341044795 7 0.007578507353146476 8 0.02433099729168079 9 0.03529988951334017 10-11 0.03898942598789832 12-13 0.030014877806540653 14-15 0.024929300503771307 16-17 0.027771240761201233 18-19 0.021738350039288576 20-21 0.0516036520428066 22-23 0.011218185226697087 24-25 0.0019444854392941617 26-27 0.0014957580302262783 28-29 0.0 30-31 0.0020442026413092468 32-33 0.0018447682372790765 34-35 0.0 36-37 4.487274090678835E-4 38-39 9.971720201508522E-5 40-41 0.0012963236261961078 42-43 0.004038546681610951 44-45 0.0019943440403017046 46-47 8.97454818135767E-4 48-49 4.487274090678835E-4 50-51 0.003589819272543068 52-53 0.0032408090654902698 54-55 0.005185294504784431 56-57 7.977376161206818E-4 58-59 0.00533487030780706 60-61 0.0025926472523922156 62-63 6.481618130980539E-4 64-65 8.97454818135767E-4 66-67 0.014957580302262782 68-69 0.040834194225177395 70-71 0.05245124825993483 72-73 0.015256731908308039 74-75 0.03161035303878201 76-77 0.025876613922914612 78-79 0.055592340123410015 80-81 0.07967404441005309 82-83 0.04696680214910513 84-85 0.017101500145587114 86-87 0.018996126983873735 88-89 0.0198935818020095 90-91 0.040285749614094424 92-93 0.025328169311831645 94-95 0.014209701287149644 96-97 0.01849754097379831 98-99 0.049260297795452095 100-101 0.06895444519343143 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1002836.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.44356602548888 #Duplication Level Percentage of deduplicated Percentage of total 1 83.2023218460858 54.450566432082915 2 10.629403978928682 13.912522022132269 3 2.8766168667380225 5.647681975452142 4 1.12272601781144 2.939007771007087 5 0.5796468855044423 1.8967079611489481 6 0.33513762500726046 1.3159560773872907 7 0.22294918122697302 1.0213412623369267 8 0.1636797632674002 0.8569429915541193 9 0.12335068988628017 0.7265258116076142 >10 0.6785164107979322 8.066540585931499 >50 0.040559404439980275 1.8125555584058823 >100 0.020501453785951904 2.542999563375391 >500 0.001988946491975963 0.9722230945215262 >1k 0.002600930027968567 3.8384288930563573 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4694 0.46807254625881 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3692 0.36815590983969465 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3617 0.36067711968856325 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3091 0.30822587142862845 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3017 0.3008467984795121 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2716 0.27083192067297146 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2416 0.2409167600684459 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2324 0.23174277748305802 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2153 0.21469113593847847 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1756 0.17510340673848962 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1523 0.15186929866897478 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1382 0.13780917318484778 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1259 0.12554395733699228 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1253 0.12494565412490177 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1181 0.11776601557981564 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1159 0.11557223713548376 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1103 0.10998807382263899 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 9.971720201508522E-5 7 0.0 0.0 0.0 0.0 9.971720201508522E-5 8 0.0 0.0 0.0 0.0 9.971720201508522E-5 9 0.0 0.0 0.0 0.0 9.971720201508522E-5 10-11 0.0 0.0 0.0 0.0 9.971720201508522E-5 12-13 0.0 0.0 0.0 4.985860100754261E-5 9.971720201508522E-5 14-15 0.0 0.0 0.0 1.9943440403017045E-4 1.4957580302262782E-4 16-17 0.0 0.0 0.0 1.9943440403017045E-4 1.9943440403017045E-4 18-19 0.0 0.0 0.0 1.9943440403017045E-4 2.49293005037713E-4 20-21 0.0 0.0 0.0 2.9915160604525564E-4 2.9915160604525564E-4 22-23 0.0 0.0 0.0 2.9915160604525564E-4 2.9915160604525564E-4 24-25 0.0 0.0 0.0 3.4901020705279827E-4 2.9915160604525564E-4 26-27 0.0 0.0 0.0 5.484446110829687E-4 2.9915160604525564E-4 28-29 0.0 0.0 0.0 9.97172020150852E-4 2.9915160604525564E-4 30-31 0.0 0.0 0.0 0.0030413746614600993 2.9915160604525564E-4 32-33 0.0 0.0 0.0 0.005733739115867401 4.487274090678835E-4 34-35 0.0 0.0 0.0 0.008376244969267158 4.985860100754261E-4 36-37 0.0 0.0 0.0 0.010968892221659374 4.985860100754261E-4 38-39 0.0 0.0 0.0 0.013262387868006334 4.985860100754261E-4 40-41 0.0 0.0 0.0 0.01760008615566254 4.985860100754261E-4 42-43 4.985860100754261E-5 0.0 0.0 0.023383683872537482 4.985860100754261E-4 44-45 9.971720201508522E-5 0.0 0.0 0.03534974811434771 4.985860100754261E-4 46-47 9.971720201508522E-5 0.0 0.0 0.061276220638269874 4.985860100754261E-4 48-49 9.971720201508522E-5 0.0 0.0 0.11407647910525748 5.983032120905113E-4 50-51 9.971720201508522E-5 0.0 0.0 0.1475814589823261 5.983032120905113E-4 52-53 9.971720201508522E-5 0.0 0.0 0.17929152922312322 5.983032120905113E-4 54-55 9.971720201508522E-5 0.0 0.0 0.22695635178633394 5.983032120905113E-4 56-57 9.971720201508522E-5 0.0 0.0 0.26474917135005127 5.983032120905113E-4 58-59 9.971720201508522E-5 0.0 0.0 0.3115165390951262 5.983032120905113E-4 60-61 9.971720201508522E-5 0.0 0.0 0.39258662433339053 5.983032120905113E-4 62-63 9.971720201508522E-5 0.0 0.0 0.4534141175625925 7.977376161206818E-4 64-65 9.971720201508522E-5 0.0 0.0 0.5141917521907869 7.977376161206818E-4 66-67 9.971720201508522E-5 0.0 0.0 0.6394864165227415 7.977376161206818E-4 68-69 9.971720201508522E-5 0.0 0.0 0.7875166029141355 7.977376161206818E-4 70-71 9.971720201508522E-5 0.0 0.0 0.9064792249181322 7.977376161206818E-4 72-73 9.971720201508522E-5 0.0 0.0 1.0420447610576407 7.977376161206818E-4 74-75 9.971720201508522E-5 0.0 0.0 1.150038490839978 7.977376161206818E-4 76-77 9.971720201508522E-5 0.0 0.0 1.2293635250429782 7.977376161206818E-4 78-79 9.971720201508522E-5 0.0 0.0 1.3423431149260696 7.977376161206818E-4 80-81 9.971720201508522E-5 0.0 0.0 1.5155518948262727 7.977376161206818E-4 82-83 9.971720201508522E-5 0.0 0.0 1.7009760319733238 7.977376161206818E-4 84-85 9.971720201508522E-5 0.0 0.0 1.9181102393611718 7.977376161206818E-4 86-87 9.971720201508522E-5 0.0 0.0 2.3262527472089154 7.977376161206818E-4 88-89 9.971720201508522E-5 0.0 0.0 2.849967492192143 7.977376161206818E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 915 0.0 28.556456 1 TACCTGG 915 0.0 28.55503 2 GTACATG 5550 0.0 24.82377 1 TACATGG 5620 0.0 24.259768 2 ACATGGG 5595 0.0 23.772635 3 GCCGATC 100 0.0016510074 23.756153 8 ACCGTGC 120 1.707602E-4 23.756151 8 GAGTACT 3575 0.0 22.592134 12-13 TAGCCCT 700 0.0 21.038012 4 GACCGCG 115 0.0037184434 20.656492 7 TATCACG 115 0.0037205943 20.65443 2 ATGGGCG 115 0.0037205943 20.65443 5 CATGGGG 1930 0.0 20.429693 4 AGTACTT 3815 0.0 20.361399 12-13 GTACACG 165 6.313948E-5 20.154722 1 GTATAGG 260 1.4868419E-8 20.099352 1 GTACTTT 4030 0.0 19.927996 14-15 GTATAGC 435 0.0 19.6583 1 GTGTAGC 630 0.0 19.606295 1 AGGACCG 150 7.714446E-4 19.002075 5 >>END_MODULE