FastQCFastQC Report
Thu 26 May 2016
SRR936539_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936539_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002836
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT70540.7034051430144111No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46480.46348555496611604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42040.41921111727141824No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA33990.33893876964927466No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA33090.329964221467917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24740.24670035778532082No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23380.23313881831126923No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21110.2105030134538449No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT18300.18248247968760595No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17960.17909209481909305No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16810.16762461658735825No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT14680.1463848525581451No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14410.1436924881037378No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14110.14070097204328524No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG13250.1321252926699879No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA12680.12644141215512805No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12150.12115640044832854No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA11260.11228156946898596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG9050.028.3515592
GCTAGAC2059.549694E-1025.4921023
GTACCTG10450.025.006711
GTACATG62250.023.3556631
TACATGG63150.022.8734682
ACATGGG61800.022.1396523
GAGTACT49200.021.81788612-13
CGATCGT550.002880705821.5875524-25
GTAAGCG1005.394413E-721.3732794-95
AGTACTT50100.021.2363412-13
GTACACG1805.155669E-621.1167761
GTACTTT51350.020.90490214-15
GTAAGGT3650.020.8233494
GTATAGG2751.2532837E-920.7328341
CATGGGG19750.019.9633984
ATGGGAT15300.019.560175
ACCTGGG13250.019.3617533
TATAACG1300.00754860718.2750432
TACTTTT58000.018.22141814-15
CATGGGA41250.018.0800324
Produced by FastQC (version 0.11.5)