##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936539_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1002836 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16544579572333 33.0 31.0 34.0 31.0 34.0 2 32.29119517049647 33.0 31.0 34.0 31.0 34.0 3 32.406620823344994 34.0 31.0 34.0 31.0 34.0 4 36.07156603871421 37.0 35.0 37.0 35.0 37.0 5 35.97244115687909 37.0 35.0 37.0 35.0 37.0 6 36.04616707018894 37.0 35.0 37.0 35.0 37.0 7 35.986126345683644 37.0 35.0 37.0 35.0 37.0 8 35.96646410779031 37.0 35.0 37.0 35.0 37.0 9 37.62335516475276 39.0 37.0 39.0 35.0 39.0 10-11 37.527474582085205 39.0 37.0 39.0 35.0 39.0 12-13 37.42168709539746 39.0 37.0 39.0 34.5 39.0 14-15 38.546188010801366 40.0 38.0 41.0 34.0 41.0 16-17 38.54208115783638 40.0 38.0 41.0 34.5 41.0 18-19 38.722407751616416 40.0 38.0 41.0 35.0 41.0 20-21 38.740251147745 40.0 38.0 41.0 34.5 41.0 22-23 38.65526965525768 40.0 38.0 41.0 34.0 41.0 24-25 38.541767547236034 40.0 38.0 41.0 34.0 41.0 26-27 38.33911427192482 40.0 38.0 41.0 34.0 41.0 28-29 38.371850432174355 40.0 38.0 41.0 34.0 41.0 30-31 38.26889640978186 40.0 38.0 41.0 34.0 41.0 32-33 38.07792799620277 40.0 37.0 41.0 34.0 41.0 34-35 37.88716001419973 40.0 37.0 41.0 33.0 41.0 36-37 37.70843687302809 40.0 37.0 41.0 33.0 41.0 38-39 37.507668751420965 40.0 37.0 41.0 33.0 41.0 40-41 37.302424823201406 40.0 36.5 41.0 32.5 41.0 42-43 37.045852961002595 39.0 36.0 41.0 31.5 41.0 44-45 36.73500203423092 39.0 36.0 41.0 31.0 41.0 46-47 36.31366245328249 39.0 36.0 40.5 30.0 41.0 48-49 35.88109022811307 39.0 35.0 40.0 29.0 41.0 50-51 35.58522779397629 39.0 35.0 40.0 28.0 41.0 52-53 35.33397085864488 38.5 35.0 40.0 27.5 41.0 54-55 35.039553825351305 38.0 34.5 40.0 27.0 41.0 56-57 34.72038698251758 38.0 34.0 40.0 26.0 41.0 58-59 34.39013657267988 38.0 34.0 40.0 26.0 41.0 60-61 34.01095044453929 37.5 33.0 40.0 24.5 41.0 62-63 33.70434148754133 37.0 33.0 39.0 24.0 41.0 64-65 33.39789058230858 36.5 33.0 39.0 23.0 40.0 66-67 32.96244351020506 36.0 32.0 39.0 22.0 40.0 68-69 32.512765796201975 35.5 31.5 38.5 21.5 40.0 70-71 31.99960262694997 35.0 31.0 38.0 19.5 40.0 72-73 31.433130142914692 35.0 31.0 37.0 18.0 39.0 74-75 30.91127013788895 34.0 30.0 36.5 15.5 39.0 76-77 29.514039184871706 33.0 28.5 35.0 13.0 37.5 78-79 30.270024211336647 34.0 30.0 36.0 12.0 38.0 80-81 30.101450286986108 34.0 30.0 35.5 7.5 37.0 82-83 29.698096697765138 34.0 29.5 35.0 7.0 37.0 84-85 29.263449357621788 34.0 29.0 35.0 2.0 36.0 86-87 28.85260650794347 34.0 29.0 35.0 2.0 36.0 88-89 28.36011172315314 33.0 28.5 35.0 2.0 35.5 90-91 27.79177004016609 33.0 27.0 35.0 2.0 35.0 92-93 27.25425244007993 32.5 26.0 34.0 2.0 35.0 94-95 26.563626555089765 32.0 25.0 34.0 2.0 35.0 96-97 25.773038662353564 32.0 24.0 34.0 2.0 35.0 98-99 24.677093762090713 31.5 13.5 34.0 2.0 35.0 100-101 20.887532956535267 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2612218907912336 1101 2 0.3744989575441693 1101 3 0.2543044341930383 1101 4 0.211252532995168 1101 5 0.18282328923466906 1101 6 0.22962412483702366 1101 7 0.2125061237968282 1101 8 0.10937524970218249 1101 9 0.22203558126087586 1101 10-11 0.25116163897350674 1101 12-13 0.2389931441737687 1101 14-15 0.3125527706325286 1101 16-17 0.22581104791556328 1101 18-19 0.08247990576366959 1101 20-21 0.20596587223602825 1101 22-23 0.17687332966020364 1101 24-25 0.08412282919113778 1101 26-27 0.048283610556488554 1101 28-29 -0.022713608694374443 1101 30-31 0.22814132655100394 1101 32-33 0.22849478058216732 1101 34-35 0.20779824306227823 1101 36-37 0.2958366823978267 1101 38-39 0.35161047816018964 1101 40-41 0.4196981485480791 1101 42-43 0.18208000769596566 1101 44-45 0.36062365192019996 1101 46-47 0.3255880960487687 1101 48-49 0.2484360735203026 1101 50-51 0.24727338775137042 1101 52-53 0.47320513648963214 1101 54-55 0.5851561558645741 1101 56-57 0.5229697407394056 1101 58-59 0.5239740970125055 1101 60-61 0.5761528683016834 1101 62-63 0.371782189492599 1101 64-65 0.5704687670045985 1101 66-67 0.6391433452334994 1101 68-69 0.497810575431231 1101 70-71 0.5279015930355264 1101 72-73 0.6240689879152193 1101 74-75 0.7093721403120021 1101 76-77 0.6904826933994208 1101 78-79 0.7166676577880757 1101 80-81 0.8636379187002206 1101 82-83 0.7833009024789241 1101 84-85 0.76735268013881 1101 86-87 0.7424723880381698 1101 88-89 0.5268840858137267 1101 90-91 0.7709254455436003 1101 92-93 0.6397112558926317 1101 94-95 0.5391731189450297 1101 96-97 0.6770405693910639 1101 98-99 0.40912723440797194 1101 100-101 0.7789337026698568 1102 1 0.24052528495656134 1102 2 0.28787838448074865 1102 3 0.3007249552386 1102 4 0.14523753451235422 1102 5 0.062690752063574 1102 6 0.12393250404721812 1102 7 0.22388719796563095 1102 8 0.06954745049112177 1102 9 0.10038802393286517 1102 10-11 0.1539315899900231 1102 12-13 0.2324619226210345 1102 14-15 0.2791551374451302 1102 16-17 0.34993703950600974 1102 18-19 0.2818955176237381 1102 20-21 0.2259992503625199 1102 22-23 0.2956913321743997 1102 24-25 0.27214761353375394 1102 26-27 0.21588975950096057 1102 28-29 0.2843551307394989 1102 30-31 0.4617394452384218 1102 32-33 0.24153690421128005 1102 34-35 0.2551149884782049 1102 36-37 0.413590215277452 1102 38-39 0.520799378849425 1102 40-41 0.5241134352965275 1102 42-43 0.5493304303410724 1102 44-45 0.5748708559602491 1102 46-47 0.5434223649811045 1102 48-49 0.6446051749117814 1102 50-51 0.620202155795063 1102 52-53 0.6955107739251503 1102 54-55 0.6067319177094745 1102 56-57 0.6634006220086448 1102 58-59 0.6706812581378259 1102 60-61 0.7423138960878859 1102 62-63 0.4838535840047129 1102 64-65 0.5897351526303254 1102 66-67 0.6195556409550349 1102 68-69 0.5552740545590638 1102 70-71 0.12836758635990364 1102 72-73 0.03727177087070643 1102 74-75 0.25444398998038764 1102 76-77 0.1931109499341659 1102 78-79 0.24808915416850041 1102 80-81 0.2660372424680446 1102 82-83 0.44889731974885194 1102 84-85 0.19574034323646927 1102 86-87 0.08070280548149356 1102 88-89 0.030308865821524478 1102 90-91 0.24787665712624474 1102 92-93 0.5306140908659707 1102 94-95 0.6659837197513063 1102 96-97 0.4968656051533422 1102 98-99 0.37741936887413985 1102 100-101 0.6530301632880011 1103 1 0.2366305869050649 1103 2 0.27278095944965486 1103 3 0.22127058147655987 1103 4 0.17194995793121137 1103 5 0.14534483562338352 1103 6 0.22003089641904694 1103 7 0.20304100514237433 1103 8 0.2068079184997842 1103 9 0.1657197987727912 1103 10-11 0.22966055032905075 1103 12-13 0.19412154535814352 1103 14-15 0.491921731945105 1103 16-17 0.4457515634361684 1103 18-19 0.3817702815465438 1103 20-21 0.22550867041871925 1103 22-23 0.21775073150186586 1103 24-25 0.3712289277352383 1103 26-27 0.2238124528539558 1103 28-29 0.1974433084538063 1103 30-31 0.4551120096126411 1103 32-33 0.4022136396666198 1103 34-35 0.29431301694565803 1103 36-37 0.5210822116844724 1103 38-39 0.35793979296338563 1103 40-41 0.2597181959948571 1103 42-43 0.3691470962400132 1103 44-45 0.5190429159353798 1103 46-47 0.5163037160336685 1103 48-49 0.5821092054981705 1103 50-51 0.4992572136051905 1103 52-53 0.7791687613957876 1103 54-55 0.5954816873902473 1103 56-57 0.5360525175358433 1103 58-59 0.2808941882589764 1103 60-61 0.2719712386646975 1103 62-63 0.4248054933510659 1103 64-65 0.42215690167449793 1103 66-67 0.21566180660061463 1103 68-69 0.16068540469034787 1103 70-71 0.12808429371628804 1103 72-73 0.16806199612290484 1103 74-75 0.09417578324585563 1103 76-77 0.07016050311487376 1103 78-79 -0.027043855182458998 1103 80-81 0.21131569840797937 1103 82-83 0.16573624600064107 1103 84-85 0.25101810821873727 1103 86-87 0.19445113566713346 1103 88-89 0.13022105661796957 1103 90-91 0.2555791077227738 1103 92-93 0.15909824490346836 1103 94-95 0.1629023364540565 1103 96-97 0.5107748708956628 1103 98-99 0.5350253732962678 1103 100-101 0.45873142774053477 1104 1 0.10889980921344034 1104 2 0.18349191244721652 1104 3 0.09516177173424012 1104 4 0.18699212332576565 1104 5 0.06262511155675554 1104 6 0.05728510798805786 1104 7 0.05743009915558872 1104 8 0.13518735510161406 1104 9 0.1529480036987252 1104 10-11 0.04536580495364717 1104 12-13 0.06891799768004603 1104 14-15 0.056813276867977436 1104 16-17 0.05031462297182543 1104 18-19 0.12632059109293436 1104 20-21 0.16286633134724582 1104 22-23 0.02301645141128006 1104 24-25 -0.016222273733184522 1104 26-27 0.14767391665277074 1104 28-29 0.15393557348171072 1104 30-31 0.08787159342939077 1104 32-33 0.10734512553420927 1104 34-35 0.21211021900285232 1104 36-37 0.22494030199518278 1104 38-39 0.17998092001626276 1104 40-41 -0.008271121720376584 1104 42-43 0.06042066496071641 1104 44-45 0.0757034064251556 1104 46-47 -0.03630826805834886 1104 48-49 0.11536564126047466 1104 50-51 0.0585196510978605 1104 52-53 0.04045856262293768 1104 54-55 -0.1768293507782701 1104 56-57 -0.04680798231760974 1104 58-59 0.27364471624834863 1104 60-61 0.011003073232949134 1104 62-63 0.11605597356004438 1104 64-65 0.11946939890869146 1104 66-67 0.24423255717606907 1104 68-69 0.13227819773967298 1104 70-71 -0.21703155609806757 1104 72-73 0.17280315787268208 1104 74-75 0.33522818326457937 1104 76-77 0.09367070086156204 1104 78-79 0.2329876221552034 1104 80-81 0.1630430718268201 1104 82-83 0.18823856607539113 1104 84-85 0.3808983622180193 1104 86-87 0.31274320149247714 1104 88-89 0.09624053947378997 1104 90-91 0.03549107635831206 1104 92-93 0.24621184102254645 1104 94-95 0.30019175800346076 1104 96-97 0.1881411387082892 1104 98-99 0.11638544056080136 1104 100-101 0.21044302400968817 1105 1 -0.010658673113539407 1105 2 0.15185189751364447 1105 3 -0.05335689229755047 1105 4 0.042607556506830235 1105 5 0.04811765552185676 1105 6 0.0440129944772778 1105 7 0.052912889387783935 1105 8 0.03262656041671619 1105 9 0.10935135867009649 1105 10-11 0.0013039518225852476 1105 12-13 -0.011007881768506422 1105 14-15 0.06807600412115988 1105 16-17 -0.005330520978809261 1105 18-19 0.077302707946167 1105 20-21 0.0980264860778135 1105 22-23 -0.050850972098146485 1105 24-25 0.02807120612114744 1105 26-27 0.01860016944059595 1105 28-29 0.05847213434285692 1105 30-31 0.05276499627028386 1105 32-33 0.1297865298371903 1105 34-35 0.011278121989370504 1105 36-37 0.03344461885272665 1105 38-39 -0.15506957851872727 1105 40-41 0.021931243804182543 1105 42-43 -5.128005073515851E-4 1105 44-45 0.006309212925948771 1105 46-47 0.020241881570562725 1105 48-49 -0.05307487562872382 1105 50-51 0.029476396517608805 1105 52-53 -0.04378417436442561 1105 54-55 -0.11434095654762899 1105 56-57 0.09944816751849572 1105 58-59 0.19152278090445662 1105 60-61 0.11091824116720517 1105 62-63 0.07525350912989381 1105 64-65 -0.1528747612636323 1105 66-67 -0.08190471048865078 1105 68-69 -0.12391659544795885 1105 70-71 0.03270798600424385 1105 72-73 0.16621205820282015 1105 74-75 -0.02870040142773078 1105 76-77 -0.33562255420734743 1105 78-79 -0.35236535937916 1105 80-81 -0.17555805913708156 1105 82-83 -0.23502653022072906 1105 84-85 -0.07916606324020137 1105 86-87 -0.19291259533276772 1105 88-89 0.08422429237401019 1105 90-91 0.17655663747160588 1105 92-93 0.0311762369654609 1105 94-95 0.2728666459528135 1105 96-97 0.16041239021581788 1105 98-99 0.26520512160005083 1105 100-101 0.09407063078765177 1106 1 0.05870686376534451 1106 2 0.20647599362288105 1106 3 0.17681769313949047 1106 4 0.15613331628074434 1106 5 0.1572369366967834 1106 6 0.11704430561654533 1106 7 0.06813650019323347 1106 8 0.04783948798651494 1106 9 -0.01139840505233991 1106 10-11 0.0910267503460176 1106 12-13 0.10523925423407121 1106 14-15 0.0696364687421962 1106 16-17 0.15024445432511158 1106 18-19 0.13850625278620043 1106 20-21 0.16332349071220165 1106 22-23 0.028351688169976796 1106 24-25 0.04867382607785942 1106 26-27 -0.0471049347957333 1106 28-29 0.12717103731699098 1106 30-31 0.14982860211871696 1106 32-33 -0.04230472778618832 1106 34-35 -0.10380596575845402 1106 36-37 0.021365440407791425 1106 38-39 0.015216212279305807 1106 40-41 0.09885374547610581 1106 42-43 -0.06397727411940224 1106 44-45 0.0767420050465688 1106 46-47 -0.13624579816598015 1106 48-49 0.01414938471745586 1106 50-51 -0.1328966777401419 1106 52-53 0.09016721976221476 1106 54-55 0.15458955897546645 1106 56-57 0.2598722634852848 1106 58-59 -0.06039396962561483 1106 60-61 0.10675284758612946 1106 62-63 0.04781824775741228 1106 64-65 0.18131679200048723 1106 66-67 -0.04425663193980256 1106 68-69 -0.011613353940596483 1106 70-71 0.07069472312328529 1106 72-73 -0.0016810439168040148 1106 74-75 -0.02591635021656913 1106 76-77 -0.2020457855442679 1106 78-79 -0.19305374435169753 1106 80-81 -0.24472951723493708 1106 82-83 0.0018414584607384654 1106 84-85 0.164776364090649 1106 86-87 0.22951856723829778 1106 88-89 0.12660112713070504 1106 90-91 0.16144306399337793 1106 92-93 -0.009553242853552746 1106 94-95 0.12199015768905852 1106 96-97 0.18333968225060815 1106 98-99 0.4787922031466145 1106 100-101 0.33202688507430267 1107 1 -0.04345203976762235 1107 2 0.10333671993247151 1107 3 -0.046007212131378594 1107 4 0.07385108883641323 1107 5 0.04483714994857024 1107 6 0.030292766388974712 1107 7 -0.04438012625013954 1107 8 -0.03232414948854512 1107 9 -0.11961362841346812 1107 10-11 -0.015166991534208307 1107 12-13 -0.06567632792550882 1107 14-15 0.011675855326060969 1107 16-17 0.06194769078467033 1107 18-19 -0.027732259589136277 1107 20-21 -0.11543396942496287 1107 22-23 -0.11439624218118638 1107 24-25 0.09154393515661496 1107 26-27 -0.03868628656136508 1107 28-29 0.015403036072761722 1107 30-31 -0.012909748924982978 1107 32-33 -0.14977016528894893 1107 34-35 -0.13364651439433572 1107 36-37 -0.0673038947433966 1107 38-39 0.0021777534490539097 1107 40-41 0.01478371594120631 1107 42-43 -0.009396500362065296 1107 44-45 -0.045380237130395074 1107 46-47 0.09556183572186683 1107 48-49 -0.040672419271963633 1107 50-51 -0.0824946640737636 1107 52-53 -0.16396956315350764 1107 54-55 -0.2960329025660897 1107 56-57 -0.1362638245824357 1107 58-59 -0.160366964954342 1107 60-61 -0.19186774761540448 1107 62-63 -0.4177364815807252 1107 64-65 -0.3430883894917969 1107 66-67 -0.1916386876839411 1107 68-69 -0.43522495403313854 1107 70-71 -0.550245967964166 1107 72-73 -0.2536771843379988 1107 74-75 -0.29539395297553384 1107 76-77 -0.4855143614695656 1107 78-79 -0.40048792143269907 1107 80-81 0.05720862891372391 1107 82-83 0.15056523626723362 1107 84-85 0.003611866953995957 1107 86-87 -0.08903828455867213 1107 88-89 -0.09917185256781735 1107 90-91 -0.10914645030168657 1107 92-93 -0.2300873937036556 1107 94-95 -0.22011337466122072 1107 96-97 -0.1806113255497941 1107 98-99 0.04281764789188358 1107 100-101 0.025047995918029642 1108 1 -0.12693202054628117 1108 2 0.03787838448074865 1108 3 -0.00975891572914378 1108 4 0.09343487796586203 1108 5 0.05986817141840817 1108 6 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-1.8583116524417527 2316 88-89 -2.064517848911059 2316 90-91 -2.3620350312845275 2316 92-93 -2.038811597697393 2316 94-95 -2.306687848876148 2316 96-97 -1.9276980821892984 2316 98-99 -1.5883217427456024 2316 100-101 -1.1799574578304828 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 8.0 5 33.0 6 44.0 7 107.0 8 261.0 9 455.0 10 903.0 11 1605.0 12 2447.0 13 3713.0 14 4633.0 15 6362.0 16 8726.0 17 12296.0 18 10554.0 19 6227.0 20 5394.0 21 5449.0 22 5851.0 23 6400.0 24 7489.0 25 8926.0 26 11009.0 27 13437.0 28 16831.0 29 21018.0 30 26313.0 31 33251.0 32 42988.0 33 56997.0 34 77609.0 35 109485.0 36 160498.0 37 210319.0 38 120023.0 39 5173.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.89733841820981 19.418536927851665 14.627729597332035 21.056395056606483 2 15.42218946742765 21.897067725634937 39.74132798530422 22.93941482163319 3 20.878823900148788 25.872182310634923 30.020981838344106 23.228011950872183 4 12.70769908848233 17.243748460611087 38.365610226229094 31.68294222467749 5 12.984573041215011 38.972466817578955 33.98208996898653 14.06087017221951 6 30.186790262814654 37.24307862900813 17.788751101875082 14.781380006302127 7 27.898906599789598 34.07230501528168 20.61695094407355 17.411837440855177 8 26.259185429756954 34.08658261626567 19.679281539592207 19.974950414385162 9 26.705263871659973 17.730316821494245 20.7185422142803 34.84587709256549 10-11 24.706183264262553 27.034280779708748 27.182610117706187 21.076925838322516 12-13 27.140230306849773 24.458535593058087 27.589805312134786 20.811428787957354 14-15 23.37632163345569 25.90795458058237 27.29288384583245 23.422839940129496 16-17 21.197492409967936 29.26738303073757 27.98142791825452 21.553696641039974 18-19 20.991076156315348 28.220496420590358 30.488552769742093 20.2998746533522 20-21 22.39198589594269 26.504489286341084 30.15260781466886 20.95091700304737 22-23 21.660008854922857 26.61622285686935 30.03902946854508 21.684738819662716 24-25 22.093459230001404 26.699161276104793 30.39115186676146 20.816227627132335 26-27 22.134038932606597 26.1711417276313 31.044822406067595 20.64999693369451 28-29 21.042704454224502 27.36368762742008 30.920406108884368 20.673201809471053 30-31 21.348202564438793 26.947248864839207 30.48134207990076 21.22320649082124 32-33 21.411505082224032 27.08935599899881 30.32636324564299 21.17277567313417 34-35 22.193187665282583 26.511625124148907 30.299413259302693 20.99577395126582 36-37 22.33289257978437 26.199408073966822 30.689111193544655 20.778588152704152 38-39 21.983860070752424 26.540635275991793 30.85782951109028 20.617675142165503 40-41 21.719464458527845 26.995105378698696 30.50475377068323 20.78067639209023 42-43 21.393712761448906 27.521601475830774 29.66773863834667 21.416947124373646 44-45 21.882550860953963 27.046828675795258 29.358146278905807 21.71247418434497 46-47 22.347073698989664 27.59538947544763 28.676423662493168 21.381113163069536 48-49 22.610306765840626 27.461291541726958 28.50889045558661 21.419511236845807 50-51 22.086350842991443 27.789923989918506 29.05664234377298 21.067082823317072 52-53 22.275299764017948 28.31330917837062 28.187166225997313 21.224224831614116 54-55 21.553268038379233 27.77019925531045 28.57172772363123 22.104804982679088 56-57 22.38699170451395 27.94819858902937 28.070267294562555 21.594542411894118 58-59 22.913878689851998 27.453934811700627 27.902520696676763 21.72966580177061 60-61 22.117361273502095 27.630571908223843 29.037070350849287 21.214996467424772 62-63 22.37261245737711 27.548087398182453 28.785877643940605 21.293422500499833 64-65 22.03135797815532 28.34064780162085 28.453730378826634 21.1742638413972 66-67 21.990156861004927 27.822797571473945 28.2573176623567 21.92972790516443 68-69 21.846980591242403 28.10134017005037 28.199862288415524 21.851816950291703 70-71 22.371914568993244 28.136089290210638 27.793547550726842 21.69844859006928 72-73 22.394614371316003 27.462189013548834 28.226984584425672 21.91621203070949 74-75 21.859941681425813 28.73645748567964 27.7653455235907 21.638255309303847 76-77 22.27524447101374 27.883232277503083 27.82026039347705 22.02126285800613 78-79 22.200753911664687 27.782442633904086 28.01205660320911 22.004746851222116 80-81 22.33300492414346 28.361440905292508 27.675022486722362 21.63053168384167 82-83 22.262641794668905 28.239819029341916 27.707068455831063 21.790470720158115 84-85 22.048811327650363 28.097550857805558 27.77565875448298 22.077979060061097 86-87 22.08006924390127 28.21145515898974 27.518168546675813 22.190307050433173 88-89 22.183431631084527 27.92369122888892 27.32283594122777 22.570041198798787 90-91 22.086431627607343 27.577125151891835 27.752888375643654 22.58355484485717 92-93 22.14601501862495 28.086952663061172 27.698448826635648 22.06858349167823 94-95 21.866313666539526 28.10354670396103 27.605357823963622 22.424781805535822 96-97 21.375023184295706 27.903371844193554 28.067606403452615 22.653998568058125 98-99 21.752147917098778 28.291428673995245 27.04780062861975 22.90862278028623 100-101 21.481044934330125 28.52404072918443 27.068543775097996 22.92637056138745 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 262.0 1 197.0 2 187.5 3 241.0 4 201.5 5 189.0 6 292.0 7 535.5 8 835.0 9 1057.0 10 1070.5 11 1014.5 12 1087.0 13 1171.5 14 1264.0 15 1414.0 16 1556.5 17 1700.5 18 1823.5 19 1887.0 20 2087.5 21 2403.0 22 2704.0 23 3117.0 24 3642.0 25 4338.5 26 5446.5 27 6891.0 28 8466.0 29 10405.5 30 12924.5 31 15247.0 32 17470.0 33 19841.0 34 22342.0 35 24259.0 36 26449.5 37 28656.0 38 29792.5 39 30571.5 40 31347.5 41 32647.5 42 33479.5 43 34268.5 44 35505.0 45 36513.5 46 37516.0 47 42647.0 48 58074.5 49 56226.5 50 39708.5 51 33581.0 52 30256.5 53 28289.0 54 26009.0 55 23705.5 56 21224.5 57 18826.0 58 17332.0 59 15424.0 60 12803.5 61 10305.5 62 8192.5 63 6165.0 64 4320.0 65 3239.0 66 2502.5 67 1892.5 68 1405.0 69 977.5 70 720.5 71 559.0 72 478.5 73 457.0 74 370.5 75 300.0 76 225.5 77 133.5 78 87.0 79 60.0 80 47.5 81 42.0 82 27.5 83 14.5 84 7.0 85 5.5 86 3.0 87 1.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012364933049870568 2 0.009872002999493437 3 0.006381900928965454 4 2.9915160604525564E-4 5 0.00458699129269392 6 0.0 7 9.971720201508522E-5 8 0.0024929300503771304 9 0.0 10-11 0.0 12-13 0.0 14-15 4.985860100754261E-4 16-17 0.004038546681610951 18-19 0.0016951924342564486 20-21 3.988688080603409E-4 22-23 3.988688080603409E-4 24-25 2.9915160604525564E-4 26-27 4.985860100754261E-5 28-29 1.4957580302262782E-4 30-31 4.487274090678835E-4 32-33 0.002692364454407301 34-35 7.977376161206818E-4 36-37 7.977376161206818E-4 38-39 0.004337698287656207 40-41 4.985860100754261E-5 42-43 0.0010968892221659373 44-45 0.0052850117067995165 46-47 0.0 48-49 0.001894626838286619 50-51 8.475962171282243E-4 52-53 5.484446110829687E-4 54-55 1.9943440403017045E-4 56-57 0.012165498645840395 58-59 0.001794909636271534 60-61 0.0026425058533997583 62-63 4.985860100754261E-5 64-65 0.0028917988584374714 66-67 5.484446110829687E-4 68-69 0.001545616631233821 70-71 0.003839112277580781 72-73 0.001894626838286619 74-75 0.006780769737025794 76-77 7.478790151131392E-4 78-79 0.006581335332995625 80-81 0.0021937784443318746 82-83 0.0021439198433243324 84-85 0.001545616631233821 86-87 3.988688080603409E-4 88-89 0.001545616631233821 90-91 0.006631193934003167 92-93 0.0014458994292187357 94-95 3.4901020705279827E-4 96-97 1.9943440403017045E-4 98-99 3.988688080603409E-4 100-101 0.0016951924342564486 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1002836.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.2971726020707 #Duplication Level Percentage of deduplicated Percentage of total 1 81.61392024521341 50.843164762476924 2 11.436312226723611 14.248998334387448 3 3.167071332695479 5.918987683680012 4 1.310043320477207 3.2644797940783357 5 0.6616572527501524 2.060968803899408 6 0.37567828136456916 1.4042216842210706 7 0.26773243435569305 1.167528157196042 8 0.18856153355749086 0.9397480321713725 9 0.14150900677061268 0.7934049917582805 >10 0.7593336472936539 8.634518339686467 >50 0.044955671650116456 1.92301809359143 >100 0.027446778900712725 3.1352370580056896 >500 0.002889134123596372 1.256294512434144 >1k 0.0027286266722854626 3.7040889779218746 >5k 1.6050745131090957E-4 0.70534077449144 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7054 0.7034051430144111 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4648 0.46348555496611604 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4204 0.41921111727141824 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3399 0.33893876964927466 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3309 0.329964221467917 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2474 0.24670035778532082 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2338 0.23313881831126923 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2111 0.2105030134538449 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1830 0.18248247968760595 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1796 0.17909209481909305 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1681 0.16762461658735825 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1468 0.1463848525581451 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1441 0.1436924881037378 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1411 0.14070097204328524 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1325 0.1321252926699879 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1268 0.12644141215512805 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1215 0.12115640044832854 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1126 0.11228156946898596 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.985860100754261E-5 0.0 14-15 0.0 0.0 0.0 9.971720201508522E-5 4.985860100754261E-5 16-17 0.0 0.0 0.0 9.971720201508522E-5 9.971720201508522E-5 18-19 0.0 0.0 0.0 9.971720201508522E-5 9.971720201508522E-5 20-21 0.0 0.0 0.0 1.9943440403017045E-4 9.971720201508522E-5 22-23 0.0 0.0 0.0 1.9943440403017045E-4 2.49293005037713E-4 24-25 0.0 0.0 0.0 2.49293005037713E-4 2.9915160604525564E-4 26-27 0.0 0.0 0.0 4.487274090678835E-4 2.9915160604525564E-4 28-29 0.0 0.0 0.0 9.473134191433096E-4 3.4901020705279827E-4 30-31 0.0 0.0 0.0 0.0030912332624676415 3.988688080603409E-4 32-33 0.0 0.0 0.0 0.005833456317882485 3.988688080603409E-4 34-35 0.0 0.0 0.0 0.008675396575312414 3.988688080603409E-4 36-37 0.0 0.0 0.0 0.011716771236772512 3.988688080603409E-4 38-39 0.0 0.0 0.0 0.014209701287149643 3.988688080603409E-4 40-41 0.0 0.0 0.0 0.018547399574805848 3.988688080603409E-4 42-43 0.0 0.0 0.0 0.024680007498733593 3.988688080603409E-4 44-45 0.0 0.0 0.0 0.03689536474558153 3.988688080603409E-4 46-47 0.0 0.0 0.0 0.06312098887554894 3.988688080603409E-4 48-49 0.0 0.0 0.0 0.11597110594354411 4.985860100754261E-4 50-51 0.0 0.0 0.0 0.14912707561355995 4.985860100754261E-4 52-53 0.0 0.0 0.0 0.18128587326342493 4.985860100754261E-4 54-55 0.0 0.0 0.0 0.22890083722562812 4.985860100754261E-4 56-57 0.0 0.0 0.0 0.26659393958733035 4.985860100754261E-4 58-59 0.0 0.0 0.0 0.3136604589384505 4.985860100754261E-4 60-61 0.0 0.0 0.0 0.3947305441767148 4.985860100754261E-4 62-63 0.0 0.0 0.0 0.4564554922240526 4.985860100754261E-4 64-65 0.0 0.0 0.0 0.5165351064381414 4.985860100754261E-4 66-67 0.0 0.0 0.0 0.642378215381179 4.985860100754261E-4 68-69 0.0 0.0 0.0 0.7916050081967541 4.985860100754261E-4 70-71 0.0 0.0 0.0 0.9112157920138487 4.985860100754261E-4 72-73 0.0 0.0 0.0 1.047230055562425 6.980204141055965E-4 74-75 0.0 0.0 0.0 1.1559716643598754 6.980204141055965E-4 76-77 0.0 0.0 0.0 1.235595850168921 6.980204141055965E-4 78-79 0.0 0.0 0.0 1.3483760056479823 6.980204141055965E-4 80-81 4.985860100754261E-5 0.0 0.0 1.5219337957552381 6.980204141055965E-4 82-83 9.971720201508522E-5 0.0 0.0 1.7072083570992667 6.980204141055965E-4 84-85 9.971720201508522E-5 0.0 0.0 1.9228468064568882 6.980204141055965E-4 86-87 9.971720201508522E-5 0.0 0.0 2.32839666705224 6.980204141055965E-4 88-89 9.971720201508522E-5 0.0 0.0 2.8498677749901278 6.980204141055965E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 905 0.0 28.351559 2 GCTAGAC 205 9.549694E-10 25.492102 3 GTACCTG 1045 0.0 25.00671 1 GTACATG 6225 0.0 23.355663 1 TACATGG 6315 0.0 22.873468 2 ACATGGG 6180 0.0 22.139652 3 GAGTACT 4920 0.0 21.817886 12-13 CGATCGT 55 0.0028807058 21.58755 24-25 GTAAGCG 100 5.394413E-7 21.37327 94-95 AGTACTT 5010 0.0 21.23634 12-13 GTACACG 180 5.155669E-6 21.116776 1 GTACTTT 5135 0.0 20.904902 14-15 GTAAGGT 365 0.0 20.823349 4 GTATAGG 275 1.2532837E-9 20.732834 1 CATGGGG 1975 0.0 19.963398 4 ATGGGAT 1530 0.0 19.56017 5 ACCTGGG 1325 0.0 19.361753 3 TATAACG 130 0.007548607 18.275043 2 TACTTTT 5800 0.0 18.221418 14-15 CATGGGA 4125 0.0 18.080032 4 >>END_MODULE