FastQCFastQC Report
Thu 26 May 2016
SRR936538_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936538_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150045
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52980.4606776256581264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42730.37155067845171275No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39350.3421605241534027No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA34440.2994665426135499No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34150.29694490215600255No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA31250.27172849758052947No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25290.2199044385219709No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24380.2119917046724259No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT23150.2012964710076562No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20840.1812103004665035No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15820.13755983461516721No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15410.1339947567269107No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG13080.11373467994730642No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC12750.11086522701285603No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA12740.11077827389363024No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC12180.10590889921698715No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT11860.10312639940176253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG1151.0310214E-733.0630231
GTACGCT600.00394289131.6798823
GTACCTG11600.027.8612941
TACCTGG11700.027.2122042
GTACACG2007.1304385E-1026.1404511
TACATGG65100.025.6942792
GTACATG66050.025.6169591
GTATAGG3650.024.740651
ACATGGG66250.024.3158043
GTGTAGC7450.022.3286341
CATGGGA44800.021.7455044
GAGTACT41050.021.706112-13
ATGGGAT14900.021.689735
TCCGTAG1150.00371054520.6643891
AGTACTT42300.020.16590912-13
GTACTTT44300.020.16554814-15
ACCTGGG15800.019.850053
ACACCGT1200.004748428719.8025116
CGGGTCG1507.6926674E-419.010416
ATGGGAG18050.018.9577485
Produced by FastQC (version 0.11.5)