FastQCFastQC Report
Thu 26 May 2016
SRR936538_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936538_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150045
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72040.626410270902443No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51940.45163450125864635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46840.407288410453504No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA37550.3265089626927642No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA36100.3139007604050276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27910.24268615575912247No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25390.22077396971422855No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24510.21312209522236086No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19680.17112373863631422No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT18320.1592981144216096No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT17500.1521679586450965No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC15980.13895108452277954No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15950.13869022516510224No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15080.1311253037924603No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13390.11643022664330525No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG13220.11495202361646718No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13220.11495202361646718No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA11650.10130038389802139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAT700.00791036227.4733981
GCACCGT1257.404291E-626.5938036
TACCTGG10800.025.0723252
GTACATG71750.023.7209321
GAGTACT56350.023.1324712-13
GTACCTG11900.023.0291711
ACATGGG70950.022.8970453
TACATGG73650.022.7045762
CATGGGG23250.022.268474
GTACTTT59850.021.62100614-15
AAGCGTC1100.002881260121.5858787
AGTACTT58400.021.38535512-13
GTGTAGC6250.020.7698861
GTAAGGT4100.019.6948154
TAAGGTG5550.019.68015
TAGCCCT7100.019.4011084
TACACCG1250.00603926618.9955755
ACTTTTT69900.018.64827516-17
TACTTTT67450.018.44560214-15
GTATAAT6750.017.806831
Produced by FastQC (version 0.11.5)