##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936538_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1150045 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63348825480742 31.0 31.0 34.0 30.0 34.0 2 31.88202722502163 33.0 31.0 34.0 30.0 34.0 3 32.16962814498563 33.0 31.0 34.0 30.0 34.0 4 35.93076879600363 37.0 35.0 37.0 35.0 37.0 5 35.78776656565613 37.0 35.0 37.0 35.0 37.0 6 35.903507254063975 37.0 35.0 37.0 35.0 37.0 7 35.85417005421527 37.0 35.0 37.0 35.0 37.0 8 35.83774026233756 37.0 35.0 37.0 35.0 37.0 9 37.50427244151316 39.0 37.0 39.0 35.0 39.0 10-11 37.39455456090849 39.0 37.0 39.0 34.0 39.0 12-13 37.2812333430431 39.0 37.0 39.0 34.0 39.0 14-15 38.414977240021045 40.0 38.0 41.0 34.0 41.0 16-17 38.354756987770045 40.0 38.0 41.0 33.5 41.0 18-19 38.44704989804747 40.0 38.0 41.0 33.5 41.0 20-21 38.52842453990931 40.0 38.0 41.0 34.0 41.0 22-23 38.44912155611303 40.0 38.0 41.0 34.0 41.0 24-25 38.35103843762636 40.0 38.0 41.0 34.0 41.0 26-27 38.142867453012705 40.0 37.5 41.0 33.5 41.0 28-29 38.0749548930694 40.0 37.5 41.0 33.5 41.0 30-31 38.07541400553892 40.0 37.0 41.0 34.0 41.0 32-33 37.912946884687116 40.0 37.0 41.0 33.0 41.0 34-35 37.74422044354786 40.0 37.0 41.0 33.0 41.0 36-37 37.51385554478303 40.0 36.5 41.0 33.0 41.0 38-39 37.355723906455836 40.0 36.0 41.0 32.5 41.0 40-41 37.129224508606185 39.0 36.0 41.0 31.5 41.0 42-43 36.90285945332574 39.0 36.0 41.0 31.0 41.0 44-45 36.61486159237248 39.0 36.0 41.0 30.5 41.0 46-47 36.190866009590934 39.0 35.0 40.5 30.0 41.0 48-49 35.70029477107418 39.0 35.0 40.0 28.0 41.0 50-51 35.52155089583451 39.0 35.0 40.0 28.0 41.0 52-53 35.27140155385224 38.5 35.0 40.0 27.0 41.0 54-55 35.03136268580795 38.0 34.0 40.0 26.5 41.0 56-57 34.770168123856024 38.0 34.0 40.0 26.0 41.0 58-59 34.42216304579386 38.0 34.0 40.0 25.5 41.0 60-61 34.04702511640849 37.5 33.0 40.0 24.0 41.0 62-63 33.77671873709289 37.0 33.0 40.0 24.0 41.0 64-65 33.35838510666974 36.5 32.5 39.0 23.0 40.5 66-67 32.96278623879935 36.0 32.0 39.0 22.0 40.0 68-69 32.51532766109152 35.5 31.5 38.5 21.5 40.0 70-71 32.034997326191586 35.0 31.0 38.0 20.0 40.0 72-73 31.603706811472595 35.0 31.0 37.0 18.5 39.0 74-75 31.237511575633995 34.5 31.0 37.0 18.0 39.0 76-77 29.79999652187523 33.5 28.5 35.5 16.0 37.5 78-79 30.576175280097736 34.0 30.0 36.0 16.5 38.0 80-81 30.408444886939208 34.0 30.0 35.5 12.5 37.0 82-83 30.07315278967345 34.0 30.0 35.0 8.5 37.0 84-85 29.625507262759285 34.0 29.5 35.0 7.0 36.5 86-87 29.24675077931733 34.0 29.0 35.0 2.0 36.0 88-89 28.831576155715645 34.0 29.0 35.0 2.0 36.0 90-91 28.36816820211383 33.5 29.0 35.0 2.0 35.0 92-93 27.94694077188284 33.0 27.5 35.0 2.0 35.0 94-95 27.458049902395125 33.0 27.0 35.0 2.0 35.0 96-97 26.790273424083402 33.0 25.5 34.0 2.0 35.0 98-99 25.974416218495797 32.0 24.5 34.0 2.0 35.0 100-101 23.179870352899236 29.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2950687616854637 1101 2 0.27517659984524556 1101 3 0.10541808364651217 1101 4 0.09286780029023589 1101 5 0.14447438394481082 1101 6 0.1151279196024646 1101 7 0.10110456368816045 1101 8 0.07780163647026939 1101 9 0.13498054683810778 1101 10-11 0.20415072922268251 1101 12-13 0.05811580539725725 1101 14-15 0.006916169157655361 1101 16-17 -0.009871647430429675 1101 18-19 0.17535582047678133 1101 20-21 0.07514721524773194 1101 22-23 0.04201860449175854 1101 24-25 -0.013818426272983686 1101 26-27 0.07932031068077805 1101 28-29 -0.027560567939993064 1101 30-31 -0.03704850131578752 1101 32-33 0.06244548319455134 1101 34-35 0.06310300465802499 1101 36-37 0.06400806491770084 1101 38-39 0.1750866530387114 1101 40-41 0.2658766820930012 1101 42-43 0.10170161570788139 1101 44-45 -0.08293394709314583 1101 46-47 -0.023146568884918395 1101 48-49 0.061972944874007396 1101 50-51 0.13700581758022423 1101 52-53 0.06144406091120658 1101 54-55 0.009154591071457219 1101 56-57 0.013909703654235273 1101 58-59 -0.13294948514160865 1101 60-61 0.007623708295398046 1101 62-63 -0.07778700267390093 1101 64-65 -0.021909943006825472 1101 66-67 -0.1820706507414087 1101 68-69 -0.08475172516258311 1101 70-71 -0.2798927287771562 1101 72-73 -0.22274103660764766 1101 74-75 -0.1521588920427206 1101 76-77 0.241691343295944 1101 78-79 -0.16938970067305803 1101 80-81 -0.1662092200683496 1101 82-83 -0.0846960945457198 1101 84-85 0.05847456423052577 1101 86-87 -0.15361316249694212 1101 88-89 -0.1336460220566451 1101 90-91 -0.1314184628640227 1101 92-93 -0.019049513554747932 1101 94-95 -0.6548027704329691 1101 96-97 -0.5824017090585514 1101 98-99 -0.45639683604602155 1101 100-101 -0.5870299122208316 1102 1 0.38796315573167206 1102 2 0.5141722250816088 1102 3 0.19416602349234324 1102 4 0.14212240966633516 1102 5 0.10598300040830111 1102 6 0.16847248943293636 1102 7 0.172088722094756 1102 8 -0.04894184924054201 1102 9 0.05485000426470066 1102 10-11 0.07638381961734808 1102 12-13 0.116897548112604 1102 14-15 0.22625220423046954 1102 16-17 0.3170240445712693 1102 18-19 0.27883228686300043 1102 20-21 0.14781591444421593 1102 22-23 0.1691512434033413 1102 24-25 0.049406479531860725 1102 26-27 -0.08338658015556177 1102 28-29 0.0501533495978137 1102 30-31 0.10832484764281247 1102 32-33 0.16936736775120664 1102 34-35 0.17379059424263232 1102 36-37 0.1345561476320185 1102 38-39 0.0557113991670235 1102 40-41 0.05250423890190348 1102 42-43 -0.05604596463229683 1102 44-45 -0.2037297151979942 1102 46-47 -0.10538512189799576 1102 48-49 -0.02346917704976903 1102 50-51 0.028089376966356383 1102 52-53 -0.045074430285573897 1102 54-55 -0.009416210532783964 1102 56-57 -0.0828022202612928 1102 58-59 -0.0032207018114647212 1102 60-61 0.20967825977707832 1102 62-63 0.07431750608729715 1102 64-65 0.008024272126959886 1102 66-67 0.025969203028502363 1102 68-69 0.08802225783326634 1102 70-71 -0.05582802709682255 1102 72-73 -0.18890338375938143 1102 74-75 -0.5768564637454254 1102 76-77 -0.19850675832743647 1102 78-79 -0.0014667095859977053 1102 80-81 -0.03825695494926862 1102 82-83 0.194558794964653 1102 84-85 0.2680766334890592 1102 86-87 0.13106722763673062 1102 88-89 -0.05495957168476551 1102 90-91 0.10770112179022107 1102 92-93 0.13533785612445115 1102 94-95 -0.1624686449421482 1102 96-97 -0.25887673275324374 1102 98-99 -0.5936035704740554 1102 100-101 -0.6991734997137691 1103 1 0.4082170468474011 1103 2 0.2897234864057836 1103 3 0.16677754329948868 1103 4 0.016727558279690413 1103 5 -0.11230978751513021 1103 6 -0.048591978247124246 1103 7 -0.018881593499358473 1103 8 0.13410369618529927 1103 9 -0.06461848893840028 1103 10-11 -0.1011715935057822 1103 12-13 0.0916500186243141 1103 14-15 0.0056166154690302506 1103 16-17 0.06198209609566874 1103 18-19 0.011927099027580823 1103 20-21 0.017328412889682454 1103 22-23 0.04933262153273432 1103 24-25 -0.03800993442353473 1103 26-27 -0.12014626370046955 1103 28-29 -0.060865658753620266 1103 30-31 0.06709002227665906 1103 32-33 0.08719905726552213 1103 34-35 -0.0721221797139151 1103 36-37 -0.11106293916353849 1103 38-39 -0.02408158486672818 1103 40-41 0.10590747041731419 1103 42-43 0.14751893831701324 1103 44-45 -0.013148272017367901 1103 46-47 0.25141129645793114 1103 48-49 0.12827285656640441 1103 50-51 -0.06398585286537184 1103 52-53 -0.12528115926111383 1103 54-55 -0.18612930591893218 1103 56-57 -0.29302482938951613 1103 58-59 -0.31005395064197927 1103 60-61 -0.2620426321900524 1103 62-63 -0.18485280376701496 1103 64-65 -0.2782657032875022 1103 66-67 -0.31834120612459316 1103 68-69 -0.31462475315847627 1103 70-71 -0.4104543173592248 1103 72-73 -0.43684996522169683 1103 74-75 -0.4979105806271775 1103 76-77 -0.6777578082351177 1103 78-79 -0.4825320191054807 1103 80-81 -0.4035478200349374 1103 82-83 -0.19775607211081692 1103 84-85 -0.11102709452027781 1103 86-87 -0.24216897528483727 1103 88-89 -0.3023206920965009 1103 90-91 -0.4297286850650295 1103 92-93 -0.253897600621265 1103 94-95 -0.27176379575576703 1103 96-97 -0.43571801732366566 1103 98-99 -0.5424967429650387 1103 100-101 -0.378961700569004 1104 1 0.19781172495982347 1104 2 0.3293461462789651 1104 3 0.0977599220898 1104 4 -3.155238990899534E-4 1104 5 -0.09043243035593918 1104 6 0.02529106285929572 1104 7 0.0961886528861271 1104 8 -0.05427243707600837 1104 9 -0.14041353592342887 1104 10-11 -0.01303002039552581 1104 12-13 -0.07009356138649281 1104 14-15 0.11228381743894289 1104 16-17 0.08623181992785334 1104 18-19 -0.017659111560718088 1104 20-21 -0.01722906164691551 1104 22-23 -0.03817121075189078 1104 24-25 0.044097311149265295 1104 26-27 0.04822096406133625 1104 28-29 0.09950900485694802 1104 30-31 0.04912719178773273 1104 32-33 0.09502592072912819 1104 34-35 0.09455908613227137 1104 36-37 -0.0993052466125306 1104 38-39 -0.17929641179345168 1104 40-41 -0.048114116902816306 1104 42-43 -0.011161977148873348 1104 44-45 0.03400050822117606 1104 46-47 0.17796282426206744 1104 48-49 0.1548380102714404 1104 50-51 0.12480368049618562 1104 52-53 0.02921465807395407 1104 54-55 0.21555349284111713 1104 56-57 0.16190336697918184 1104 58-59 0.09056651448788244 1104 60-61 0.044924826238776916 1104 62-63 -0.30289714634162124 1104 64-65 -0.00581459837292897 1104 66-67 0.04556086257914416 1104 68-69 -0.11417047535555724 1104 70-71 -5.346615105494834E-4 1104 72-73 0.05774566183739793 1104 74-75 0.023804968958941686 1104 76-77 0.24019528946745794 1104 78-79 0.31451091708150614 1104 80-81 0.37574251384829793 1104 82-83 0.25097501586870763 1104 84-85 0.35464025830782475 1104 86-87 0.296903295520206 1104 88-89 0.22998388571955886 1104 90-91 0.191499495244571 1104 92-93 0.2790254957673852 1104 94-95 0.22473556431353003 1104 96-97 0.2583123781681067 1104 98-99 0.31263705851531753 1104 100-101 0.2894001741603027 1105 1 0.24497591798354534 1105 2 0.25815003436619577 1105 3 0.06971837522793578 1105 4 -0.004500012401635445 1105 5 -0.16901069767561694 1105 6 -0.09631708635097169 1105 7 -0.021824972043610558 1105 8 0.003388962994236522 1105 9 -0.03784744497871628 1105 10-11 0.0018782335790064053 1105 12-13 -0.05310548079251731 1105 14-15 0.07600431225876747 1105 16-17 0.0638788155451877 1105 18-19 0.005775493895640693 1105 20-21 -0.011953848912604315 1105 22-23 -0.06071301382244343 1105 24-25 -0.19031691367880654 1105 26-27 -0.23743803965940202 1105 28-29 -0.1687762114201874 1105 30-31 -0.24928522665504005 1105 32-33 -0.22235151597769232 1105 34-35 -0.14242660216691405 1105 36-37 -0.051918296479200876 1105 38-39 -0.1623615496215578 1105 40-41 -0.10689513120539118 1105 42-43 -0.19756532919988246 1105 44-45 -0.6012670359919241 1105 46-47 -0.29722142551784003 1105 48-49 -0.21231867135213633 1105 50-51 -0.386591397357293 1105 52-53 -0.6296613384410037 1105 54-55 -0.3784023642517056 1105 56-57 -0.48126888686153535 1105 58-59 -0.34682657350345636 1105 60-61 -0.4588377162029076 1105 62-63 -0.7128525898512805 1105 64-65 -0.47207462561063096 1105 66-67 -0.4635242823346104 1105 68-69 -0.41024533796332463 1105 70-71 -0.2785335069242123 1105 72-73 -0.46872315394120534 1105 74-75 -0.6206032193795359 1105 76-77 -0.4656175813825847 1105 78-79 -0.4827390589702034 1105 80-81 -0.6663084024969024 1105 82-83 -0.2810059613330296 1105 84-85 -0.31884596901795703 1105 86-87 -0.5300027729511179 1105 88-89 -0.2974861966574913 1105 90-91 -0.30390192624482637 1105 92-93 -0.2173455146882617 1105 94-95 -0.09121713920765373 1105 96-97 -0.3441391664585822 1105 98-99 -0.4523557113819088 1105 100-101 -0.27755214872245304 1106 1 0.17074618437653655 1106 2 0.3091873558696534 1106 3 0.0616493381712786 1106 4 -0.044636078083946984 1106 5 -0.07390652411186949 1106 6 -0.16858032323547434 1106 7 -0.1509328755506445 1106 8 -0.05890329682168982 1106 9 -0.024583523903437765 1106 10-11 -0.13645714129133069 1106 12-13 -0.05750499303069745 1106 14-15 -0.04686007450766283 1106 16-17 0.03409748071105412 1106 18-19 -0.10596334386286088 1106 20-21 -0.1050200719588048 1106 22-23 -0.01433287963276797 1106 24-25 -0.09171040062400238 1106 26-27 -0.12705185810606423 1106 28-29 -0.14256729045525418 1106 30-31 -0.12702419683756005 1106 32-33 -0.04188019681372879 1106 34-35 -0.31349747108920667 1106 36-37 -0.2440757596763632 1106 38-39 -0.17210023288537712 1106 40-41 -0.05630115429131166 1106 42-43 -0.20446241366434492 1106 44-45 -0.08331144218053765 1106 46-47 -0.07510385505722184 1106 48-49 -0.21627842548487308 1106 50-51 -0.1037003050798262 1106 52-53 0.014637255657298454 1106 54-55 -0.04332627561589675 1106 56-57 -0.15363088999557561 1106 58-59 -0.29198868257670796 1106 60-61 -0.0784761986236191 1106 62-63 -0.5003539692681755 1106 64-65 -0.1034113909331893 1106 66-67 -0.14864423642762148 1106 68-69 -0.2904697415034647 1106 70-71 -0.26424219614710154 1106 72-73 -0.21333597920770586 1106 74-75 -0.21848167619826953 1106 76-77 -0.015782283759595117 1106 78-79 -0.1909819025553645 1106 80-81 -0.2636585426456577 1106 82-83 -0.05180618866093312 1106 84-85 -0.10085809685128311 1106 86-87 -0.1253566209724859 1106 88-89 -0.30744889722470603 1106 90-91 -0.40213544497179043 1106 92-93 0.10685414763048584 1106 94-95 -0.15078477477674923 1106 96-97 -0.14955834366399756 1106 98-99 -0.11143613690443388 1106 100-101 -0.26147918308648244 1107 1 0.4984760009048834 1107 2 0.613316262989116 1107 3 0.3603106628556887 1107 4 0.23950729690412942 1107 5 -0.004351848287519999 1107 6 0.2098315499560428 1107 7 0.06569563409990309 1107 8 0.17221374982526072 1107 9 0.1209067937537256 1107 10-11 0.3268672092444618 1107 12-13 0.3679167214912198 1107 14-15 0.532684982568874 1107 16-17 0.5140209281974606 1107 18-19 0.47045482523631676 1107 20-21 0.3172664077384866 1107 22-23 0.40503856302041186 1107 24-25 0.36555334385118243 1107 26-27 0.44211608046114037 1107 28-29 0.47673697995080033 1107 30-31 0.5674803979031324 1107 32-33 0.37907197972606355 1107 34-35 0.29273961117095837 1107 36-37 0.520581701610169 1107 38-39 0.5150201842980024 1107 40-41 0.7528783903682879 1107 42-43 0.5632249531835711 1107 44-45 0.31355173311914797 1107 46-47 0.6611222570608106 1107 48-49 0.6035302329780379 1107 50-51 0.5064538333665496 1107 52-53 0.3845369345024139 1107 54-55 0.43445640427853505 1107 56-57 0.4552771833930862 1107 58-59 0.36624687340281525 1107 60-61 0.37863531525599825 1107 62-63 0.14971121563436895 1107 64-65 0.38927478350797173 1107 66-67 0.47024984605513254 1107 68-69 0.43988217747646985 1107 70-71 0.2462660871051483 1107 72-73 0.30837894018559453 1107 74-75 0.5909963676187004 1107 76-77 0.47161516925958225 1107 78-79 0.6246807576246098 1107 80-81 0.42417215149405507 1107 82-83 0.18850153177532647 1107 84-85 0.19361668772056362 1107 86-87 0.3701007067644859 1107 88-89 0.6707507275646343 1107 90-91 0.4810440678903056 1107 92-93 0.40933077645811267 1107 94-95 0.5327647816479413 1107 96-97 0.5605433288852097 1107 98-99 0.5127259986165704 1107 100-101 0.8703294594776665 1108 1 0.31973584263693056 1108 2 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-1.5159683826864594 2316 48-49 -1.3042240582834452 2316 50-51 -1.5569311428694874 2316 52-53 -1.5684732612767434 2316 54-55 -1.5123325144750837 2316 56-57 -1.5986398026515403 2316 58-59 -1.4669441192968478 2316 60-61 -1.2613638991583755 2316 62-63 -1.547769299036446 2316 64-65 -1.5551270772077004 2316 66-67 -1.6108545751085472 2316 68-69 -1.5141105614678096 2316 70-71 -1.4227537825998624 2316 72-73 -1.5254349096889932 2316 74-75 -1.514961177089539 2316 76-77 -1.408897257021625 2316 78-79 -1.1920959845517984 2316 80-81 -1.0886362610057319 2316 82-83 -1.0702776681618253 2316 84-85 -1.2918785959600143 2316 86-87 -1.3035428954822876 2316 88-89 -1.2675201984724787 2316 90-91 -1.2948047854352502 2316 92-93 -1.8104586865941137 2316 94-95 -1.8318008175574967 2316 96-97 -1.919634101239751 2316 98-99 -2.1408924648901717 2316 100-101 -2.907735867578463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 12.0 5 24.0 6 61.0 7 149.0 8 347.0 9 684.0 10 1187.0 11 1989.0 12 3153.0 13 4314.0 14 5571.0 15 7713.0 16 10049.0 17 13476.0 18 11277.0 19 6723.0 20 5809.0 21 5919.0 22 6457.0 23 6991.0 24 8163.0 25 9945.0 26 12122.0 27 15216.0 28 19170.0 29 23769.0 30 30160.0 31 37977.0 32 48405.0 33 63386.0 34 85960.0 35 118907.0 36 172904.0 37 237137.0 38 163520.0 39 11390.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.53872258932123 19.486620737241207 14.81382388869918 21.160832784738382 2 15.33004900404816 21.81545432013949 39.786069283984325 23.06842739182802 3 20.710294319697663 25.91789031752022 30.055375165003728 23.316440197778384 4 12.760278743826492 17.1668523393453 38.22822172535575 31.844647191472447 5 12.891495550174122 38.95360616323709 34.01458203809416 14.140316248494624 6 30.390289075644866 37.08150550630627 17.71756757344278 14.810637844606081 7 28.006904077666526 33.86519657926429 20.66510440895791 17.462794934111272 8 26.264624427739786 34.11023046924251 19.70009869179032 19.925046411227388 9 26.674023194802775 17.564363099446172 20.670800583304853 35.09081312244619 10-11 24.72602075664226 26.971983184954627 27.236408425261178 21.065587633141934 12-13 27.08807409809894 24.377914564933658 27.660965720412197 20.87304561655521 14-15 23.307914038146333 25.832076136151194 27.333495645822552 23.526514179879918 16-17 21.288732180045127 29.09273115399832 27.94694990196036 21.671586763996192 18-19 21.016960205904986 28.079118643183527 30.479459499410893 20.424461651500593 20-21 22.455208274458826 26.416401097348363 30.188644792160307 20.939745836032504 22-23 21.71928035139846 26.460055294041435 30.025342740015837 21.79532161454427 24-25 22.08417931472247 26.562004095491915 30.387941341425773 20.965875248359847 26-27 22.073092792021182 26.125586390097777 30.998656574307965 20.802664243573076 28-29 21.146216017634092 27.17954514823333 30.886878339543234 20.78736049458934 30-31 21.460256537899188 26.79696307403757 30.475385952456623 21.267394435606622 32-33 21.462546761113536 26.948184077482146 30.316505999161734 21.272763162242587 34-35 22.228840363901178 26.40474767127159 30.285055922089498 21.081356042737738 36-37 22.327723536797876 26.147944543187375 30.65054973339409 20.873782186620662 38-39 21.96808207689535 26.50251925253262 30.80922591188942 20.720172758682605 40-41 21.78182608695652 26.882217391304348 30.48795652173913 20.848 42-43 21.471512864106167 27.387697244554985 29.689974389188674 21.450815502150178 44-45 21.938035287043636 26.95944568185662 29.32604537899377 21.776473652105974 46-47 22.344316292529548 27.44142730934311 28.757486800275473 21.45676959785187 48-49 22.607307651502108 27.28916322729099 28.60790943354222 21.495619687664686 50-51 22.067266326707625 27.735762349239007 28.965414696265047 21.231556627788326 52-53 22.283467613781866 28.143808121347615 28.26615397926207 21.306570285608448 54-55 21.666368818176323 27.57507412514072 28.62889252104615 22.129664535636813 56-57 22.395342618820393 27.8450264238556 28.014982576124837 21.744648381199173 58-59 22.936087347248616 27.382578114232626 27.891173321055398 21.790161217463364 60-61 22.232497834729887 27.469755785358252 28.988140580814143 21.309605799097717 62-63 22.423917523621697 27.404995817398582 28.782515160843197 21.388571498136525 64-65 22.096277984689277 28.099378395830755 28.494909107512083 21.309434511967893 66-67 22.036036811581727 27.64220749492291 28.233415375491788 22.08834031800358 68-69 21.87558829245303 27.989264763233074 28.204169490832747 21.930977453481148 70-71 22.435646327948408 27.95166914993198 27.780369494226896 21.832315027892715 72-73 22.336560743275264 27.399449586755303 28.23515601563417 22.028833654335266 74-75 22.004138968475146 28.615749818485366 27.68487320061389 21.695238012425598 76-77 22.283723033312175 27.810404945957934 27.84288235959062 22.062989661139273 78-79 22.27412007129263 27.661853095268015 28.064841680881564 21.99918515255779 80-81 22.36517012718774 28.24150451135993 27.690183715621263 21.70314164583107 82-83 22.295127017401057 28.01057307952029 27.8725177765226 21.82178212655606 84-85 22.062235424659725 28.00515845823666 27.746496387687873 22.186109729415733 86-87 22.11903160346163 28.16357464400831 27.56609907719788 22.151294675332185 88-89 22.266880574472054 27.76706257586109 27.44894067363563 22.51711617603122 90-91 22.153111150636285 27.543107928223247 27.781592585231955 22.52218833590851 92-93 22.22516902959556 28.007109438483923 27.748244487815654 22.019477044104864 94-95 21.9678088672144 27.815789290608933 27.738785540861123 22.477616301315543 96-97 21.423319562947878 27.686605680425313 28.172765073397848 22.717309683228958 98-99 21.804165063297745 28.05441236257226 27.262098097915295 22.879324476214702 100-101 21.575843695729496 28.28358020751171 27.2552832349634 22.885292861795392 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 313.0 1 238.0 2 203.0 3 271.5 4 254.0 5 229.5 6 315.5 7 603.5 8 968.5 9 1167.5 10 1206.0 11 1220.0 12 1249.5 13 1323.5 14 1447.5 15 1581.5 16 1771.0 17 1868.5 18 1963.5 19 2144.0 20 2381.0 21 2743.5 22 3153.5 23 3585.0 24 4089.5 25 4931.0 26 6222.5 27 7830.5 28 9560.5 29 11610.0 30 14410.5 31 17341.5 32 20126.5 33 22631.5 34 25251.5 35 27446.0 36 30171.5 37 32846.0 38 34113.0 39 35076.5 40 35944.0 41 37199.0 42 38067.5 43 39156.0 44 40635.0 45 41759.5 46 42990.0 47 48901.0 48 65842.5 49 63436.0 50 45091.5 51 38603.0 52 34917.5 53 32757.0 54 30671.0 55 27797.5 56 24736.0 57 22057.0 58 20088.5 59 17893.0 60 15069.0 61 12233.5 62 9807.5 63 7365.5 64 5194.0 65 3890.0 66 2891.0 67 2212.5 68 1683.0 69 1172.5 70 876.5 71 686.0 72 589.0 73 555.0 74 436.0 75 333.0 76 257.5 77 162.0 78 98.5 79 85.5 80 67.0 81 45.5 82 34.0 83 21.0 84 14.0 85 8.0 86 3.5 87 2.0 88 2.0 89 1.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.2007356233886501 2 0.01217343669160772 3 0.006173671465029629 4 0.014955936506832343 5 0.0 6 0.0 7 0.0 8 0.0 9 0.003999843484385394 10-11 3.04335917290193E-4 12-13 1.7390623845153885E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.999608710963485E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 4.3476559612884713E-5 32-33 0.004434609080514241 34-35 6.521483941932708E-4 36-37 0.002869452934450391 38-39 1.3042967883865412E-4 40-41 0.003912890365159624 42-43 0.01217343669160772 44-45 0.0016955858249025036 46-47 0.001826015503741158 48-49 0.011043046141672718 50-51 3.912890365159624E-4 52-53 0.00230425765948289 54-55 0.01143433517818868 56-57 0.003695507567095201 58-59 0.0012608202287736567 60-61 0.006347577703481168 62-63 0.0037389841267080857 64-65 0.017173241047089463 66-67 0.00230425765948289 68-69 4.3476559612884713E-5 70-71 0.0011738671095478871 72-73 0.0 74-75 0.0 76-77 0.0013042967883865415 78-79 0.011869100774317526 80-81 0.014782030268380804 82-83 0.012477772608897913 84-85 0.007738827611093479 86-87 0.010738710224382525 88-89 0.028564099665665258 90-91 0.024825115538957172 92-93 0.002130351421031351 94-95 0.008782265041802712 96-97 0.00986917903212483 98-99 0.010651757105156754 100-101 0.011651717976253103 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1150045.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.052036108091535 #Duplication Level Percentage of deduplicated Percentage of total 1 81.18823803748113 50.37895478254109 2 11.668434965034168 14.48100295550436 3 3.2283465383330343 6.009764278982213 4 1.3748978122084878 3.4126083475238858 5 0.6967730615421444 2.1618093586979312 6 0.40436568787289373 1.5055028558857244 7 0.27419973166480677 1.191025615506552 8 0.180216965175782 0.8946263704302642 9 0.13834103106355292 0.7725908389307578 >10 0.7670215907107903 8.718297301533386 >50 0.046643905343483436 1.9786776456576747 >100 0.027170958133319878 3.069416144400324 >500 0.0023932937478718867 0.9900292143720167 >1k 0.002674857718209756 3.3526287116535123 >5k 2.8156397033786906E-4 1.0830655783803287 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7204 0.626410270902443 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5194 0.45163450125864635 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4684 0.407288410453504 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3755 0.3265089626927642 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3610 0.3139007604050276 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2791 0.24268615575912247 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2539 0.22077396971422855 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2451 0.21312209522236086 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1968 0.17112373863631422 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1832 0.1592981144216096 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1750 0.1521679586450965 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1598 0.13895108452277954 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1595 0.13869022516510224 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1508 0.1311253037924603 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1339 0.11643022664330525 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1322 0.11495202361646718 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1322 0.11495202361646718 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1165 0.10130038389802139 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 8.695311922576943E-5 0.0 7 0.0 0.0 0.0 8.695311922576943E-5 0.0 8 0.0 0.0 0.0 8.695311922576943E-5 0.0 9 0.0 0.0 0.0 8.695311922576943E-5 0.0 10-11 0.0 0.0 0.0 8.695311922576943E-5 0.0 12-13 0.0 0.0 0.0 8.695311922576943E-5 0.0 14-15 0.0 0.0 0.0 8.695311922576943E-5 0.0 16-17 0.0 0.0 0.0 8.695311922576943E-5 0.0 18-19 0.0 0.0 0.0 8.695311922576943E-5 0.0 20-21 0.0 0.0 0.0 8.695311922576943E-5 0.0 22-23 0.0 0.0 0.0 3.04335917290193E-4 0.0 24-25 0.0 0.0 0.0 6.08671834580386E-4 0.0 26-27 0.0 0.0 0.0 9.564843114834637E-4 0.0 28-29 0.0 0.0 0.0 0.0014347264672251955 0.0 30-31 0.0 0.0 0.0 0.0029564060536761607 0.0 32-33 0.0 0.0 0.0 0.005217187153546165 0.0 34-35 0.0 0.0 0.0 0.00747796825341617 0.0 36-37 0.0 0.0 0.0 0.009086600959092906 0.0 38-39 0.0 0.0 0.0 0.01208648357238195 0.0 40-41 0.0 0.0 0.0 0.015608084901025611 0.0 42-43 0.0 0.0 0.0 0.021651326687216588 0.0 44-45 0.0 0.0 0.0 0.03065097452708372 0.0 46-47 0.0 0.0 0.0 0.054389176075718776 0.0 48-49 0.0 0.0 0.0 0.09990913399040907 0.0 50-51 0.0 0.0 0.0 0.12864713989452586 0.0 52-53 0.0 0.0 0.0 0.15451569286419226 0.0 54-55 0.0 0.0 0.0 0.1905577607832737 0.0 56-57 0.0 0.0 0.0 0.2184697120547457 0.0 58-59 0.0 0.0 0.0 0.25577260020260073 0.0 60-61 0.0 0.0 0.0 0.3222047832910886 0.0 62-63 0.0 0.0 0.0 0.3718550143690029 0.0 64-65 0.0 0.0 0.0 0.4224182531987879 0.0 66-67 0.0 0.0 0.0 0.531761800625193 0.0 68-69 0.0 0.0 0.0 0.649061558460756 0.0 70-71 0.0 0.0 0.0 0.752709676577873 0.0 72-73 0.0 0.0 0.0 0.8627488489580842 0.0 74-75 0.0 0.0 0.0 0.9553973974931416 0.0 76-77 0.0 0.0 0.0 1.0165254403088575 0.0 78-79 0.0 0.0 0.0 1.109478324761205 0.0 80-81 0.0 0.0 0.0 1.2571247212065613 0.0 82-83 0.0 0.0 0.0 1.4229443195701037 0.0 84-85 0.0 0.0 0.0 1.6211539548452452 0.0 86-87 0.0 0.0 0.0 1.9751835797729655 0.0 88-89 0.0 0.0 0.0 2.4510780012956013 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTAAT 70 0.007910362 27.473398 1 GCACCGT 125 7.404291E-6 26.593803 6 TACCTGG 1080 0.0 25.072325 2 GTACATG 7175 0.0 23.720932 1 GAGTACT 5635 0.0 23.13247 12-13 GTACCTG 1190 0.0 23.029171 1 ACATGGG 7095 0.0 22.897045 3 TACATGG 7365 0.0 22.704576 2 CATGGGG 2325 0.0 22.26847 4 GTACTTT 5985 0.0 21.621006 14-15 AAGCGTC 110 0.0028812601 21.585878 7 AGTACTT 5840 0.0 21.385355 12-13 GTGTAGC 625 0.0 20.769886 1 GTAAGGT 410 0.0 19.694815 4 TAAGGTG 555 0.0 19.6801 5 TAGCCCT 710 0.0 19.401108 4 TACACCG 125 0.006039266 18.995575 5 ACTTTTT 6990 0.0 18.648275 16-17 TACTTTT 6745 0.0 18.445602 14-15 GTATAAT 675 0.0 17.80683 1 >>END_MODULE