FastQCFastQC Report
Thu 26 May 2016
SRR936537_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936537_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207414
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55650.46090239139185074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43010.356215846428814No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40410.33468222167375894No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA36930.30586029315545454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35550.29443090770854075No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32110.26594026572492946No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25610.21210620383729195No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25390.2102841278964796No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT22920.1898271843791773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20870.17284874947615317No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16680.13814648496704526No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16390.1357446575905199No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA13430.1112294540232265No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG13320.11031841605282032No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC12830.10626015600282918No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC12360.10236753922018463No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC12280.10170496615079833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG11700.029.6525151
TACCTGG12000.028.91242
GTACATG69600.024.9917341
TACATGG69200.024.8625132
ACATGGG69350.023.7122173
GAGTACT42550.023.6837712-13
GTATATA7750.021.4630221
GTACTTT47000.021.34023314-15
CATGGGG21000.021.0495194
AGTACTT45850.020.37219212-13
CATGGGA47200.019.636824
ATGGGAG18150.019.37915
ACCTGGG17450.018.7930033
GTACTAG2403.0747215E-617.8219721
CATGGGT9350.017.7924184
ATGGGAT17300.017.5837865
ACTGCGT1650.001457855717.2833468
ACCGTGC2202.902028E-517.2833468
ACTTTTT58900.017.22761316-17
GTGTAGC7500.017.1090951
Produced by FastQC (version 0.11.5)