##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936537_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1207414 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.515096727385966 31.0 31.0 34.0 30.0 34.0 2 31.56741846624273 33.0 31.0 34.0 30.0 34.0 3 31.614804035732565 33.0 31.0 34.0 30.0 34.0 4 35.232195419301085 37.0 35.0 37.0 33.0 37.0 5 35.21546379286641 37.0 35.0 37.0 33.0 37.0 6 35.34847782119472 37.0 35.0 37.0 33.0 37.0 7 35.21741755520476 37.0 35.0 37.0 32.0 37.0 8 35.175696985458174 37.0 35.0 37.0 32.0 37.0 9 36.68101661898901 39.0 37.0 39.0 32.0 39.0 10-11 36.58486318694334 39.0 37.0 39.0 32.0 39.0 12-13 36.49449484600974 39.0 37.0 39.0 32.0 39.0 14-15 37.55219212300007 40.0 37.0 41.0 32.0 41.0 16-17 37.444008848663344 40.0 37.0 41.0 32.0 41.0 18-19 37.43107252359174 40.0 37.0 41.0 32.0 41.0 20-21 37.34769515675651 40.0 37.0 41.0 32.0 41.0 22-23 37.211079629687916 39.0 36.5 41.0 31.0 41.0 24-25 37.34694851972894 39.5 37.0 41.0 32.0 41.0 26-27 37.31901692377262 39.5 37.0 41.0 31.5 41.0 28-29 37.22672670682964 39.0 37.0 41.0 31.0 41.0 30-31 37.05811801088939 39.0 36.0 41.0 31.0 41.0 32-33 36.88933953060011 39.0 36.0 41.0 30.5 41.0 34-35 36.66028636408059 39.0 35.5 41.0 30.0 41.0 36-37 36.46890296120469 39.0 35.0 40.5 30.0 41.0 38-39 36.255776394840545 39.0 35.0 40.0 30.0 41.0 40-41 36.09816806828478 39.0 35.0 40.0 30.0 41.0 42-43 35.88191705579031 38.0 35.0 40.0 29.0 41.0 44-45 35.61525334309525 38.0 35.0 40.0 27.5 41.0 46-47 35.32358370865337 38.0 35.0 40.0 27.0 41.0 48-49 35.220660022163074 38.5 35.0 40.0 26.0 41.0 50-51 34.32053131734434 37.5 33.5 39.5 25.0 40.5 52-53 34.46985458177559 38.0 33.5 39.5 25.0 40.5 54-55 34.9029081988448 38.0 34.0 40.0 26.0 41.0 56-57 34.81627511358987 38.0 34.0 40.0 25.5 41.0 58-59 34.5813474085939 38.0 34.0 40.0 24.5 41.0 60-61 34.24572806013513 38.0 34.0 40.0 23.0 41.0 62-63 33.93386195621386 37.5 33.0 40.0 23.0 41.0 64-65 33.592141138002376 37.0 33.0 40.0 22.0 41.0 66-67 33.197195411018924 36.5 32.5 39.0 21.5 41.0 68-69 32.79839889217783 36.0 32.0 39.0 20.5 41.0 70-71 32.333428716248115 35.5 31.5 39.0 18.5 40.0 72-73 31.9278056242515 35.0 31.0 38.0 18.0 40.0 74-75 31.490285022370124 35.0 31.0 37.0 15.5 39.5 76-77 31.03034915944324 35.0 31.0 37.0 11.5 39.0 78-79 30.56451639619882 34.5 30.0 36.0 7.5 39.0 80-81 30.057787552571032 34.0 29.5 36.0 7.0 37.5 82-83 29.557528320857635 34.0 29.0 35.0 2.0 37.0 84-85 29.219144386266848 34.0 29.0 35.0 2.0 37.0 86-87 28.884226537045286 34.0 29.0 35.0 2.0 36.0 88-89 28.448952886085472 34.0 28.5 35.0 2.0 36.0 90-91 28.000960316842445 34.0 27.0 35.0 2.0 35.5 92-93 27.635033633865433 34.0 27.0 35.0 2.0 35.0 94-95 27.190426813006972 33.0 26.0 35.0 2.0 35.0 96-97 26.602076007069655 33.0 25.0 35.0 2.0 35.0 98-99 25.887781241562546 33.0 24.0 35.0 2.0 35.0 100-101 23.44331728802217 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3794025041405007 1101 2 0.4335334097871204 1101 3 0.32579792658221507 1101 4 0.3815176728745584 1101 5 0.39093695003690243 1101 6 0.1987173081112914 1101 7 0.45738960792590433 1101 8 0.49484554206541986 1101 9 0.6086667274705562 1101 10-11 0.4960612371427615 1101 12-13 0.5154193245515089 1101 14-15 0.7801026315381279 1101 16-17 0.8705935573954946 1101 18-19 0.834591955441681 1101 20-21 0.7228889933653093 1101 22-23 0.8115089969677953 1101 24-25 0.8039166909266058 1101 26-27 0.8023293111413565 1101 28-29 0.4890129319714376 1101 30-31 0.696065730052112 1101 32-33 0.6359428149459347 1101 34-35 0.5878978368252419 1101 36-37 0.4828163068165594 1101 38-39 0.44882201690566603 1101 40-41 0.43299827869230967 1101 42-43 0.33948186936759583 1101 44-45 0.4721631335741634 1101 46-47 0.609109512064208 1101 48-49 0.7024448995431669 1101 50-51 0.7748036801070128 1101 52-53 0.8706715660217554 1101 54-55 0.8159038652965265 1101 56-57 0.7495823172840659 1101 58-59 0.8420401178372927 1101 60-61 0.6835686975294024 1101 62-63 0.6483285223626822 1101 64-65 0.6437187569340068 1101 66-67 0.6972575683746172 1101 68-69 0.7837583319586869 1101 70-71 0.6890436787171481 1101 72-73 0.6273432702783417 1101 74-75 0.6058879715234475 1101 76-77 0.6146255684235769 1101 78-79 0.5677402508342411 1101 80-81 0.6567038063546562 1101 82-83 0.6879773819417316 1101 84-85 0.505094139065708 1101 86-87 0.4046380218859227 1101 88-89 0.7565912576204497 1101 90-91 0.7384127758262018 1101 92-93 0.6693316714223911 1101 94-95 0.8319185701015819 1101 96-97 0.6421769258449999 1101 98-99 0.7093777402349204 1101 100-101 1.054958731787604 1102 1 0.34135868246704604 1102 2 0.3291798477818446 1102 3 0.26960978029364924 1102 4 0.3252740236870366 1102 5 0.5191682861197648 1102 6 0.3742068009855686 1102 7 0.44686881995551886 1102 8 0.25283240922563266 1102 9 0.4841890951388379 1102 10-11 0.38665584396876795 1102 12-13 0.44198962964667743 1102 14-15 0.49339173333044783 1102 16-17 0.3561499953048255 1102 18-19 0.4983551800747179 1102 20-21 0.624351708225575 1102 22-23 0.3445225482140515 1102 24-25 0.24904801932445508 1102 26-27 0.23583939986060898 1102 28-29 0.2736271014345917 1102 30-31 0.0868029366339016 1102 32-33 0.18340355731855595 1102 34-35 0.007961067435516611 1102 36-37 0.2013421071256687 1102 38-39 0.16258267986644626 1102 40-41 0.25325149999517294 1102 42-43 0.36200067631143185 1102 44-45 0.186159470382826 1102 46-47 0.41623074747334954 1102 48-49 0.45546340335578606 1102 50-51 0.5658519163956655 1102 52-53 0.6454307688293497 1102 54-55 0.70807283319877 1102 56-57 0.5914564794037673 1102 58-59 0.6629269260790451 1102 60-61 0.4583938633976743 1102 62-63 0.4757155739633063 1102 64-65 0.6081426795373446 1102 66-67 0.6907921210639429 1102 68-69 0.5589950731700171 1102 70-71 0.6148676832940012 1102 72-73 0.40762629236848724 1102 74-75 0.3379543444516919 1102 76-77 0.523608285674733 1102 78-79 0.5732728224970209 1102 80-81 0.7752377186259203 1102 82-83 0.8698072271313393 1102 84-85 0.7964081488512988 1102 86-87 0.8419304196965491 1102 88-89 0.7920093652277629 1102 90-91 0.794456828050162 1102 92-93 0.6280458400316569 1102 94-95 1.013742011110537 1102 96-97 0.8703723985162277 1102 98-99 1.0361608776003273 1102 100-101 0.9105173471183576 1103 1 0.11142971176137806 1103 2 0.09021880882080424 1103 3 0.1254518346793958 1103 4 0.22102697623610368 1103 5 0.2978742080295689 1103 6 0.20100459865715692 1103 7 0.08188332001856224 1103 8 0.1844593583283114 1103 9 0.34152318499720025 1103 10-11 0.33754686233550757 1103 12-13 0.2423836530989476 1103 14-15 0.008713466613343712 1103 16-17 0.21097494381909598 1103 18-19 0.23120727943167907 1103 20-21 0.4166918923133309 1103 22-23 0.31902450257037174 1103 24-25 0.36199930751846665 1103 26-27 0.2633023091196307 1103 28-29 0.04926804625029746 1103 30-31 -0.019506692248548063 1103 32-33 0.2976388366023812 1103 34-35 7.940435209192742E-4 1103 36-37 0.23016150778972388 1103 38-39 0.11402007742034925 1103 40-41 0.26258827972030474 1103 42-43 0.20929820079342676 1103 44-45 0.241710472778486 1103 46-47 0.19004447419970205 1103 48-49 0.16606841323264376 1103 50-51 0.2949686734250392 1103 52-53 0.20576048765421007 1103 54-55 0.16293981088127651 1103 56-57 0.19438065878425448 1103 58-59 0.1166254928844026 1103 60-61 0.057035270534228744 1103 62-63 0.04909009588151747 1103 64-65 0.10770977910444657 1103 66-67 0.010116081892753925 1103 68-69 0.2259275743258229 1103 70-71 0.27209459877335007 1103 72-73 0.3501913325063448 1103 74-75 0.2454092681687534 1103 76-77 0.2568239373369856 1103 78-79 0.2349737429358072 1103 80-81 0.12355865335462468 1103 82-83 0.055750571812549055 1103 84-85 0.0677547634017941 1103 86-87 0.26326547622577934 1103 88-89 0.3919988579357039 1103 90-91 0.48436247256745446 1103 92-93 0.2620012050549825 1103 94-95 0.40426548440100163 1103 96-97 0.5011556120664302 1103 98-99 0.3679675013972421 1103 100-101 0.9911681687885974 1104 1 0.1189268381488624 1104 2 0.3567888306087923 1104 3 0.33155161336834027 1104 4 0.38026027926068906 1104 5 0.36387379628807537 1104 6 0.28653531628826556 1104 7 0.38485986790924187 1104 8 0.3529474092470082 1104 9 0.5435192734530006 1104 10-11 0.5673741024665802 1104 12-13 0.20517465997846074 1104 14-15 0.4590402864249725 1104 16-17 0.32066030469335516 1104 18-19 0.5227091065345562 1104 20-21 0.5220162245200939 1104 22-23 0.3004965641943542 1104 24-25 0.2877923387936576 1104 26-27 -0.17749226618617087 1104 28-29 0.3098356490569998 1104 30-31 0.36768514497632054 1104 32-33 0.047352333145468606 1104 34-35 -0.06841326832179817 1104 36-37 -0.02562774918843047 1104 38-39 0.15176647897123985 1104 40-41 0.3186726181031858 1104 42-43 0.2838611543524152 1104 44-45 0.40610727148836645 1104 46-47 0.6425672486511402 1104 48-49 0.5704281552079706 1104 50-51 0.2899647275690498 1104 52-53 0.4350134341792895 1104 54-55 0.5607610121370925 1104 56-57 0.5675595604638204 1104 58-59 0.6383654438185431 1104 60-61 0.550222235484739 1104 62-63 0.6776838271172565 1104 64-65 0.38823955316130565 1104 66-67 0.49878761511515535 1104 68-69 0.6096367647391077 1104 70-71 0.4897703180342674 1104 72-73 0.5129836260780358 1104 74-75 0.05894484091833263 1104 76-77 0.34186656973760776 1104 78-79 0.39864956513210714 1104 80-81 0.48241624226504953 1104 82-83 0.5371698790574833 1104 84-85 0.4441079346691019 1104 86-87 0.4626088343207897 1104 88-89 0.40990875517189096 1104 90-91 0.5665302057589692 1104 92-93 0.6193971116582908 1104 94-95 0.5999494899595135 1104 96-97 0.7913222815924108 1104 98-99 0.9583469040289572 1104 100-101 1.4415305870836441 1105 1 0.28924942898996164 1105 2 0.2821706992948627 1105 3 0.3006581554522185 1105 4 0.25560249923527323 1105 5 0.37176263487366157 1105 6 0.30035844677947665 1105 7 0.3141973114458523 1105 8 0.45641892585437915 1105 9 0.45977217738412435 1105 10-11 0.4344600476723457 1105 12-13 0.17587319311558502 1105 14-15 0.1429115345552603 1105 16-17 0.2735141287720779 1105 18-19 0.31326536912523295 1105 20-21 0.4383955581056611 1105 22-23 0.22867359731798587 1105 24-25 0.4212053723895508 1105 26-27 0.5341247133164231 1105 28-29 0.2988058237437414 1105 30-31 0.2032861552980023 1105 32-33 0.29499665120157914 1105 34-35 0.2908442642361635 1105 36-37 0.4022362310959764 1105 38-39 0.3209198279161569 1105 40-41 0.20236500545762226 1105 42-43 0.1429908628112102 1105 44-45 -0.13016432729828153 1105 46-47 0.10109623032535353 1105 48-49 0.370823387322595 1105 50-51 0.27889004761735237 1105 52-53 0.3859041125022671 1105 54-55 0.40749550149607927 1105 56-57 0.450204022612553 1105 58-59 0.5522694056219137 1105 60-61 0.2628362429608231 1105 62-63 0.12921292871370582 1105 64-65 0.3951002211746939 1105 66-67 0.5516089486319302 1105 68-69 0.5415327819261009 1105 70-71 0.7724400661138873 1105 72-73 0.5828859777625688 1105 74-75 0.7069328431159754 1105 76-77 0.4253480240876719 1105 78-79 0.505349918484395 1105 80-81 0.4643110044517975 1105 82-83 0.4630853552557035 1105 84-85 0.4832272219777032 1105 86-87 0.7496211156602364 1105 88-89 0.47005208549632016 1105 90-91 0.4384936861814275 1105 92-93 0.45712627702734565 1105 94-95 0.533497459322632 1105 96-97 0.9645120831815213 1105 98-99 0.9357094884742061 1105 100-101 1.2794942515728103 1106 1 0.01090168970869243 1106 2 0.14950869681024415 1106 3 0.27070545997786155 1106 4 0.2653526804272843 1106 5 0.28506870262316397 1106 6 0.01719662785366438 1106 7 0.24132656735418578 1106 8 0.2607604861989472 1106 9 0.4462161751161986 1106 10-11 0.3332236817791454 1106 12-13 0.4255631417717112 1106 14-15 0.5541185100004569 1106 16-17 0.7175247467814572 1106 18-19 0.6769190415111481 1106 20-21 0.44912059962992856 1106 22-23 0.3736306214141578 1106 24-25 0.18157591924754257 1106 26-27 0.0833171597398703 1106 28-29 0.28092461837771765 1106 30-31 0.26223141269584005 1106 32-33 0.2826445633121395 1106 34-35 0.11831923133758693 1106 36-37 0.1324405441494818 1106 38-39 0.32696438274133044 1106 40-41 0.22752334465536705 1106 42-43 0.0441389206117222 1106 44-45 0.20813982071008041 1106 46-47 0.3847419292110601 1106 48-49 0.21759569760745734 1106 50-51 0.24146324761019144 1106 52-53 0.3324124978264038 1106 54-55 0.4029898953259874 1106 56-57 0.32556259344153204 1106 58-59 0.4568774681139587 1106 60-61 0.41832028596722637 1106 62-63 0.6356303866818962 1106 64-65 0.4151680905986339 1106 66-67 0.4819867747275808 1106 68-69 0.27091476202601683 1106 70-71 0.3820270430107229 1106 72-73 0.4124668746025115 1106 74-75 0.3342892596272229 1106 76-77 0.7505119384240899 1106 78-79 0.8425582361705253 1106 80-81 0.6320011824249541 1106 82-83 0.6642794868437036 1106 84-85 0.7531157402425919 1106 86-87 1.0233488532374082 1106 88-89 1.1313442658909771 1106 90-91 0.9951476141821942 1106 92-93 1.0097784651640822 1106 94-95 1.0412566322665917 1106 96-97 1.075839187086327 1106 98-99 1.2916639469546745 1106 100-101 1.7500343773184817 1107 1 0.12017530143182142 1107 2 0.24305268015178783 1107 3 0.04612408150770975 1107 4 0.11587908944082415 1107 5 0.15881370206155054 1107 6 0.15039841293535972 1107 7 0.21933921672301437 1107 8 0.20797486016751066 1107 9 0.3190755459310637 1107 10-11 0.28534357429407464 1107 12-13 0.19794254507402798 1107 14-15 0.2108585758393815 1107 16-17 0.10106296273659865 1107 18-19 0.2825097342023355 1107 20-21 0.5436906161328636 1107 22-23 0.4326026873913307 1107 24-25 0.5443720648189654 1107 26-27 0.19778357929454415 1107 28-29 0.29962650282262615 1107 30-31 0.41203279427648454 1107 32-33 0.27299823154270086 1107 34-35 0.29257206318911244 1107 36-37 0.3695476274504159 1107 38-39 0.22304342401594823 1107 40-41 -0.0026067131480971284 1107 42-43 0.04190648845951728 1107 44-45 0.060627033096785965 1107 46-47 0.15877054581594052 1107 48-49 0.3092793208221636 1107 50-51 0.1743358380523219 1107 52-53 0.4185739025630042 1107 54-55 0.3181477532279544 1107 56-57 0.35511558859433023 1107 58-59 0.31495332112552177 1107 60-61 0.3240948005995392 1107 62-63 0.35157958616002105 1107 64-65 0.4703054926394685 1107 66-67 0.4238387753842332 1107 68-69 0.4070072605355648 1107 70-71 0.704440143402131 1107 72-73 0.6809754327991122 1107 74-75 0.6804939089614841 1107 76-77 0.7199939320821223 1107 78-79 0.5823951076982148 1107 80-81 0.7293117875037076 1107 82-83 0.8040981057814669 1107 84-85 0.6669543355059915 1107 86-87 0.6634353208320434 1107 88-89 0.6800722007778326 1107 90-91 0.6248333456250315 1107 92-93 0.5391575746969046 1107 94-95 0.6308503879367748 1107 96-97 0.7626252840129801 1107 98-99 0.7512814417921092 1107 100-101 1.0956423073667843 1108 1 0.19786086470629272 1108 2 0.23713091811823617 1108 3 0.32423256465278527 1108 4 0.3555358923963965 1108 5 0.4541969372880814 1108 6 0.2785890936728421 1108 7 0.11622543050746259 1108 8 0.23512658101387984 1108 9 0.35418850133223856 1108 10-11 0.18806316560410608 1108 12-13 0.3698658803518171 1108 14-15 0.5207810626258933 1108 16-17 0.5328334370078665 1108 18-19 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62-63 -2.1020336133713684 2316 64-65 -2.6701052788887836 2316 66-67 -2.7129922685541246 2316 68-69 -3.28928877947628 2316 70-71 -3.3006659369047853 2316 72-73 -2.8113147560598755 2316 74-75 -2.7339973497122507 2316 76-77 -2.3143848093728785 2316 78-79 -3.0124592249871363 2316 80-81 -2.301819531934477 2316 82-83 -1.839495718645324 2316 84-85 -1.5844738543056636 2316 86-87 -2.2983093075650416 2316 88-89 -2.6321485981716606 2316 90-91 -2.5212032868401764 2316 92-93 -2.7846609301372176 2316 94-95 -3.176619165470285 2316 96-97 -4.463529980737203 2316 98-99 -6.105490833141271 2316 100-101 -6.6940782662907985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2489.0 3 939.0 4 933.0 5 971.0 6 1142.0 7 1310.0 8 1648.0 9 2069.0 10 2669.0 11 3487.0 12 4438.0 13 5535.0 14 6287.0 15 7673.0 16 9518.0 17 12298.0 18 10421.0 19 7341.0 20 7470.0 21 8134.0 22 8731.0 23 9938.0 24 11499.0 25 13299.0 26 15410.0 27 18310.0 28 21891.0 29 26253.0 30 32386.0 31 40136.0 32 50325.0 33 64905.0 34 86482.0 35 117800.0 36 165800.0 37 219175.0 38 180131.0 39 28163.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.084805864496204 18.6529819584554 14.237956838156343 22.024255338892058 2 14.890493776229222 20.55940209386437 40.932331300439216 23.61777282946719 3 20.166972208024376 25.55173479286284 29.866950752476708 24.414342246636075 4 12.10608527758097 16.306405969679265 38.83945921534019 32.748049537399574 5 12.937668541234695 38.33839682168731 34.405367463399514 14.31856717367848 6 30.231667846080672 36.23325549753317 18.68357509939394 14.851501556992213 7 27.884295082673592 33.287454196822324 21.097055504087688 17.73119521641639 8 25.856877474351265 33.55630459315824 20.437043555210867 20.149774377279634 9 26.38967835593465 17.290212136930318 21.24832830928474 35.07178119785029 10-11 24.410302943668597 26.48394334333961 27.84691017346286 21.25884353952894 12-13 26.59556044622578 23.947223849530133 27.966628357030345 21.49058734721374 14-15 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30.119317311156852 21.486103115668627 40-41 21.65688182280108 26.819403268774206 30.055048287862835 21.46866662056188 42-43 21.32082152241297 27.105435887667017 29.310062800622 22.263679789298013 44-45 21.63021399509271 26.97464567092172 29.015871043886076 22.37926929009949 46-47 22.01858607208242 27.112176937484755 28.598043994187556 22.27119299624527 48-49 22.090180329185863 27.039729071403666 28.60254612585856 22.26754447355191 50-51 21.795942639226677 27.41727026558076 28.972665054676934 21.81412204051563 52-53 21.929107478442937 27.70567933628022 28.354441073500652 22.010772111776188 54-55 21.3834514101507 27.196251580443047 28.79058886283059 22.629708146575663 56-57 22.016206690178034 27.57079813703827 28.064868178269446 22.348126994514246 58-59 22.381559406043106 27.116366946549014 28.152610840947474 22.349462806460405 60-61 22.03142886014886 27.049825312391807 29.021956468079964 21.896789359379373 62-63 22.11701446105157 27.137445629734298 28.786102415503752 21.95943749371038 64-65 21.790845385961553 27.703652354248298 28.561685189740377 21.943817070049768 66-67 21.667909766290368 27.498909785754783 28.22712734654898 22.60605310140587 68-69 21.522477992516585 27.642244096477558 28.428400194499186 22.406877716506667 70-71 21.907873022157304 27.430488978083723 28.01064650561625 22.650991494142726 72-73 21.807325111128964 27.048048627566608 28.463674680983054 22.680951580321373 74-75 21.465748080734627 28.164644767149564 28.025788433139 22.343818718976813 76-77 21.788669871788237 27.394512806474737 28.094068067903077 22.72274925383395 78-79 21.790781955516202 27.301001334754087 28.411129107536947 22.497087602192757 80-81 21.90778125433563 27.769285405152438 27.968450064917988 22.354483275593942 82-83 21.797987888235156 27.55838565572124 28.244895694123894 22.398730761919712 84-85 21.66683095773964 27.507254348260073 28.076916386336393 22.74899830766389 86-87 21.80456016346287 27.591689036243274 28.0843304739479 22.519420326345962 88-89 21.989584519655857 27.173180655781838 27.846024827357716 22.991209997204592 90-91 21.667411399253833 27.083943303142327 28.310909221169844 22.937736076433996 92-93 21.786483170852726 27.417432267994013 28.34559703649953 22.450487524653727 94-95 21.410810103389608 27.373728337632258 28.242749391606576 22.972712167371558 96-97 20.915760548826825 27.251123889828772 28.606625100171662 23.226490461172737 98-99 21.296114126236535 27.49213128891124 27.886364172937267 23.325390411914963 100-101 21.28185434392293 27.2910644199914 28.224168744236454 23.202912491849215 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 117.0 1 98.0 2 133.5 3 211.0 4 197.0 5 194.0 6 319.0 7 571.0 8 853.0 9 1070.5 10 1072.0 11 1002.0 12 1033.0 13 1113.0 14 1252.5 15 1345.5 16 1418.5 17 1518.5 18 1654.0 19 1807.5 20 2000.0 21 2282.5 22 2719.0 23 3259.0 24 3855.0 25 4798.0 26 6257.0 27 8018.5 28 9655.5 29 11664.0 30 14602.5 31 17602.5 32 20901.5 33 23682.5 34 26295.0 35 28888.5 36 31650.5 37 34629.0 38 35870.5 39 36651.5 40 37809.5 41 39190.5 42 40240.5 43 41481.5 44 43213.5 45 44831.5 46 46385.0 47 51420.0 48 67096.5 49 66023.5 50 48628.0 51 41163.5 52 37291.5 53 35191.5 54 33140.0 55 30065.0 56 26975.0 57 24080.0 58 21515.5 59 18915.0 60 15856.0 61 13159.5 62 10701.0 63 7951.5 64 5614.5 65 4190.5 66 3194.5 67 2422.5 68 1798.0 69 1287.0 70 952.0 71 742.5 72 612.0 73 537.0 74 421.5 75 335.5 76 247.0 77 154.5 78 113.0 79 80.0 80 48.5 81 37.5 82 28.0 83 18.0 84 12.0 85 9.5 86 7.0 87 4.5 88 3.5 89 3.5 90 2.5 91 1.5 92 1.0 93 1.0 94 2.0 95 1.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03495072941012776 2 0.02219619782444133 3 0.022113376190768037 4 0.05375124025396425 5 0.060956722383540364 6 0.04969298020397312 7 0.05134941287743889 8 0.0440611091141895 9 0.04571754178765527 10-11 0.05561472701161325 12-13 0.048119369164180636 14-15 0.04252890889123366 16-17 0.051432234511112174 18-19 0.05362700780345433 20-21 0.011512207080587105 22-23 0.021036694953015286 24-25 0.005300584555090466 26-27 0.010518347476507643 28-29 0.00563187108978362 30-31 0.008406395817838785 32-33 0.003851205965807917 34-35 0.008075109283145632 36-37 0.008861914803041873 38-39 0.01068399074385422 40-41 0.0016150218566291263 42-43 0.0017806651239757035 44-45 0.0021947732923421463 46-47 3.726973515297984E-4 48-49 9.110379704061739E-4 50-51 2.8987571785650984E-4 52-53 0.0030644004459116757 54-55 0.00650149824335315 56-57 0.016274451016801195 58-59 0.010021417674467913 60-61 0.00960730950610147 62-63 0.005590460272946976 64-65 8.282163367328854E-5 66-67 0.006832784778046304 68-69 0.01747536470506388 70-71 0.005756103540293552 72-73 0.0027331139112185213 74-75 0.004223903317337715 76-77 0.006253033342333285 78-79 0.006253033342333285 80-81 0.010062828491304555 82-83 0.006253033342333285 84-85 0.017144078170370728 86-87 0.0037269735152979837 88-89 0.0063358549760065734 90-91 0.003768384332134628 92-93 0.01598457529894469 94-95 0.01126374217956724 96-97 0.009234612154571671 98-99 0.021492213938218374 100-101 0.03934027599481205 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1207414.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.841687520237244 #Duplication Level Percentage of deduplicated Percentage of total 1 81.86863495809035 52.26631810702776 2 11.333301532783114 14.470741900571307 3 3.156762033845663 6.045990460215703 4 1.251142920250095 3.1950030143105463 5 0.6782774117602226 2.165118728681571 6 0.3725211650368409 1.4269427887774035 7 0.25563237165368846 1.142400139382035 8 0.1643922702715572 0.8396063959535313 9 0.12518693117659735 0.7192930447614396 >10 0.7229047446766039 8.462850492691597 >50 0.04422734088894285 1.9457753995325744 >100 0.022210549352063215 2.498256956801305 >500 0.002337942266469363 1.0210917909972352 >1k 0.002337942266469363 3.3391542416491524 >5k 1.2988568147052017E-4 0.46145653864703706 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5565 0.46090239139185074 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4301 0.356215846428814 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4041 0.33468222167375894 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3693 0.30586029315545454 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3555 0.29443090770854075 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3211 0.26594026572492946 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2561 0.21210620383729195 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2539 0.2102841278964796 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2292 0.1898271843791773 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2087 0.17284874947615317 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1668 0.13814648496704526 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1639 0.1357446575905199 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1343 0.1112294540232265 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1332 0.11031841605282032 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1283 0.10626015600282918 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1236 0.10236753922018463 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1228 0.10170496615079833 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.242324505099328E-4 0.0 22-23 0.0 0.0 0.0 4.1410816836644264E-4 0.0 24-25 0.0 0.0 0.0 6.625730693863083E-4 0.0 26-27 0.0 0.0 0.0 9.524487872428181E-4 0.0 28-29 0.0 0.0 0.0 0.0019048975744856362 0.0 30-31 0.0 0.0 0.0 0.0031886328964216082 0.0 32-33 0.0 0.0 0.0 0.005093530470907244 0.0 34-35 0.0 0.0 0.0 0.007246892946412746 0.0 36-37 0.0 0.0 0.0 0.010849634011200798 0.0 38-39 0.0 0.0 0.0 0.013996856090785763 0.0 40-41 0.0 0.0 0.0 0.017806651239757033 0.0 42-43 0.0 0.0 0.0 0.023769808864233806 0.0 44-45 0.0 0.0 0.0 0.033211475102988705 0.0 46-47 0.0 0.0 0.0 0.05693987315038586 4.141081683664427E-5 48-49 0.0 0.0 0.0 0.10091816063090209 8.282163367328854E-5 50-51 0.0 0.0 0.0 0.13102382447114247 8.282163367328854E-5 52-53 0.0 0.0 0.0 0.1569884066277184 2.0705408418322135E-4 54-55 0.0 0.0 0.0 0.1979437044791596 3.3128653469315415E-4 56-57 0.0 0.0 0.0 0.22850488730460305 3.3128653469315415E-4 58-59 0.0 0.0 0.0 0.27119943946318326 3.3128653469315415E-4 60-61 0.0 0.0 0.0 0.3389061249910966 3.3128653469315415E-4 62-63 0.0 0.0 0.0 0.390669646036902 3.3128653469315415E-4 64-65 0.0 0.0 0.0 0.4433856158699502 3.3128653469315415E-4 66-67 0.0 0.0 0.0 0.553289923754404 3.3128653469315415E-4 68-69 0.0 0.0 0.0 0.6793444502051492 3.3128653469315415E-4 70-71 0.0 0.0 0.0 0.77657704813759 3.726973515297984E-4 72-73 0.0 0.0 0.0 0.8857359613189842 4.1410816836644264E-4 74-75 0.0 0.0 0.0 0.976218596107052 4.1410816836644264E-4 76-77 0.0 0.0 0.0 1.0394943242334445 4.1410816836644264E-4 78-79 0.0 0.0 0.0 1.130680942907735 4.1410816836644264E-4 80-81 0.0 0.0 0.0 1.280132580871184 4.1410816836644264E-4 82-83 0.0 0.0 0.0 1.456625482228962 4.1410816836644264E-4 84-85 0.0 0.0 0.0 1.6583375710402564 4.1410816836644264E-4 86-87 0.0 0.0 0.0 2.0180733369001853 4.1410816836644264E-4 88-89 0.0 0.0 0.0 2.4846490101986562 4.1410816836644264E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 1170 0.0 29.652515 1 TACCTGG 1200 0.0 28.9124 2 GTACATG 6960 0.0 24.991734 1 TACATGG 6920 0.0 24.862513 2 ACATGGG 6935 0.0 23.712217 3 GAGTACT 4255 0.0 23.68377 12-13 GTATATA 775 0.0 21.463022 1 GTACTTT 4700 0.0 21.340233 14-15 CATGGGG 2100 0.0 21.049519 4 AGTACTT 4585 0.0 20.372192 12-13 CATGGGA 4720 0.0 19.63682 4 ATGGGAG 1815 0.0 19.3791 5 ACCTGGG 1745 0.0 18.793003 3 GTACTAG 240 3.0747215E-6 17.821972 1 CATGGGT 935 0.0 17.792418 4 ATGGGAT 1730 0.0 17.583786 5 ACTGCGT 165 0.0014578557 17.283346 8 ACCGTGC 220 2.902028E-5 17.283346 8 ACTTTTT 5890 0.0 17.227613 16-17 GTGTAGC 750 0.0 17.109095 1 >>END_MODULE