FastQCFastQC Report
Thu 26 May 2016
SRR936537_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936537_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207414
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT74190.6144537002221276No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55290.4579208125796123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49040.406157291533807No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38990.322921549692152No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA37160.3077651907299402No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28960.2398514511178436No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27040.2239496974525722No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24270.2010081049250713No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21160.17525057685267853No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT19530.1617506505639325No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT18480.1530543790282372No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC16900.13996856090785761No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16080.13317718694664796No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15620.12936739179767667No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14750.12216190966810059No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14650.1213336933313677No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13180.10915891318139428No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA12790.10592886946813604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG12250.028.6925282
GTACCTG13350.027.757251
GTACATG74650.023.1014861
GAGTACT59900.022.7992312-13
TACATGG76600.022.4467242
GTAAGGT4900.022.2948684
ACATGGG74700.021.9367183
GTACTTT63600.021.28613914-15
TAAGGTG6050.021.1982275
AGTACTT62450.020.65125812-13
CCTATAC2551.19271135E-820.4892313
GTGTAGC6550.020.3085941
CATGGGA51000.019.1853714
CTGATCG1250.00602862819.0014679
GGTAAGG5250.018.9991053
ACCTGGG18000.018.735233
TACACGG2552.6764246E-718.6265742
ACTTTTT73650.018.57307816-17
CATGGGG23050.018.33974
GTATAGA4151.8189894E-1218.3161831
Produced by FastQC (version 0.11.5)