##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936537_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1207414 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03287853213562 31.0 31.0 34.0 31.0 34.0 2 32.17741222149155 33.0 31.0 34.0 30.0 34.0 3 32.28744241825919 34.0 31.0 34.0 30.0 34.0 4 35.99227274157828 37.0 35.0 37.0 35.0 37.0 5 35.80785546631064 37.0 35.0 37.0 35.0 37.0 6 35.92578270584903 37.0 35.0 37.0 35.0 37.0 7 35.87822652379383 37.0 35.0 37.0 35.0 37.0 8 35.859671993202 37.0 35.0 37.0 35.0 37.0 9 37.523109720443856 39.0 37.0 39.0 35.0 39.0 10-11 37.392621751942585 39.0 37.0 39.0 34.0 39.0 12-13 37.30980094648563 39.0 37.0 39.0 34.0 39.0 14-15 38.41539852941907 40.0 38.0 41.0 34.0 41.0 16-17 38.35987656263717 40.0 38.0 41.0 34.0 41.0 18-19 38.54257735954693 40.0 38.0 41.0 34.0 41.0 20-21 38.586118348801655 40.0 38.0 41.0 34.0 41.0 22-23 38.49942480375414 40.0 38.0 41.0 34.0 41.0 24-25 38.37555966718955 40.0 38.0 41.0 34.0 41.0 26-27 38.158230317024646 40.0 37.5 41.0 33.5 41.0 28-29 38.08663432757944 40.0 37.5 41.0 33.5 41.0 30-31 38.074407369800255 40.0 37.0 41.0 34.0 41.0 32-33 37.93213802390895 40.0 37.0 41.0 33.0 41.0 34-35 37.756884134190926 40.0 37.0 41.0 33.0 41.0 36-37 37.50127462494223 40.0 36.5 41.0 33.0 41.0 38-39 37.33694904978739 40.0 36.0 41.0 32.0 41.0 40-41 37.125772932896254 39.0 36.0 41.0 31.5 41.0 42-43 36.888803260522074 39.0 36.0 41.0 31.0 41.0 44-45 36.618441975991665 39.0 36.0 41.0 31.0 41.0 46-47 36.222597634282856 39.0 35.0 40.5 30.0 41.0 48-49 35.8390369831723 39.0 35.0 40.0 29.0 41.0 50-51 35.52494463373789 39.0 35.0 40.0 28.0 41.0 52-53 35.258574109626025 38.0 35.0 40.0 27.0 41.0 54-55 35.0155178753932 38.0 34.0 40.0 26.5 41.0 56-57 34.71805072659419 38.0 34.0 40.0 26.0 41.0 58-59 34.406877011530426 38.0 34.0 40.0 25.5 41.0 60-61 34.03879820840242 37.5 33.0 40.0 24.5 41.0 62-63 33.657499416107484 37.0 33.0 39.5 23.0 41.0 64-65 33.25569233088237 36.0 32.5 39.0 22.5 40.0 66-67 32.802471646013714 36.0 32.0 39.0 22.0 40.0 68-69 32.526486772556886 35.5 32.0 38.5 21.5 40.0 70-71 32.08407389677443 35.0 31.0 38.0 20.0 40.0 72-73 31.62657754506739 35.0 31.0 37.0 18.5 39.5 74-75 31.10154470628964 34.5 30.5 37.0 17.5 39.0 76-77 29.670285419913967 33.0 28.5 35.0 14.0 37.5 78-79 30.436184274822057 34.0 30.0 36.0 14.5 38.0 80-81 30.22986150566417 34.0 30.0 35.5 10.5 37.0 82-83 29.883793794009343 34.0 30.0 35.0 7.5 37.0 84-85 29.459580144010257 34.0 29.0 35.0 6.0 36.0 86-87 29.09101145091907 34.0 29.0 35.0 2.0 36.0 88-89 28.666091332384752 34.0 29.0 35.0 2.0 35.5 90-91 28.17556240030346 33.0 28.5 35.0 2.0 35.0 92-93 27.71971958251271 33.0 27.0 35.0 2.0 35.0 94-95 27.17012474594464 33.0 26.0 34.5 2.0 35.0 96-97 26.41310519838266 32.0 25.0 34.0 2.0 35.0 98-99 25.497641239872983 32.0 23.0 34.0 2.0 35.0 100-101 22.273994669599656 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28142761439461594 1101 2 0.3366081036375661 1101 3 0.2942148005976861 1101 4 0.19142663567863138 1101 5 0.17340876093726365 1101 6 0.16108668971540396 1101 7 0.11219533081217037 1101 8 0.13133946544954256 1101 9 0.14929525744361882 1101 10-11 0.270920335883865 1101 12-13 0.2939140810470491 1101 14-15 0.4624118061099125 1101 16-17 0.4578952286281961 1101 18-19 0.43072655897965006 1101 20-21 0.3272754588615996 1101 22-23 0.27193352976124885 1101 24-25 0.37106544672222697 1101 26-27 0.2559025258779073 1101 28-29 0.15727153339364008 1101 30-31 0.27327158893279346 1101 32-33 0.29180554434552164 1101 34-35 0.21792659208571763 1101 36-37 0.041483804184949236 1101 38-39 0.08799856060866063 1101 40-41 0.03034055716840811 1101 42-43 0.09327633293927562 1101 44-45 -0.016805373454133132 1101 46-47 -0.09839671802403416 1101 48-49 -0.0686968131730552 1101 50-51 -0.08519773899748628 1101 52-53 -0.12324495986540995 1101 54-55 -0.2893936423448835 1101 56-57 -0.2881159270175715 1101 58-59 -0.1352042231791799 1101 60-61 -0.28027653851049195 1101 62-63 -0.26534257656383176 1101 64-65 -0.21963155998362538 1101 66-67 -0.4286790693317428 1101 68-69 -0.3142412925205136 1101 70-71 -0.27142336530175726 1101 72-73 -0.48880156008954145 1101 74-75 -0.18483551342932358 1101 76-77 -0.4107122757543884 1101 78-79 -0.5572144068863132 1101 80-81 -0.5078441399865881 1101 82-83 0.017276560356542348 1101 84-85 -0.33973334116293685 1101 86-87 -0.26047300662461126 1101 88-89 -0.314831312107529 1101 90-91 -0.23980320772545483 1101 92-93 -0.35086796825480704 1101 94-95 0.06405851644029426 1101 96-97 0.1280944206703758 1101 98-99 0.3764185763822674 1101 100-101 0.5037553200577456 1102 1 0.2138810133067608 1102 2 0.28218793815354104 1102 3 0.25980428407916634 1102 4 0.19561198120553058 1102 5 0.18290018360465155 1102 6 0.14837140250924818 1102 7 0.0992469314303932 1102 8 0.04126936101869916 1102 9 0.13251929276054142 1102 10-11 0.20101870409863665 1102 12-13 0.1343571222643476 1102 14-15 0.2391883258219849 1102 16-17 0.29958345603638037 1102 18-19 0.1263392682896196 1102 20-21 0.0058485647184340905 1102 22-23 -0.008008789918370951 1102 24-25 0.03373910675415459 1102 26-27 -0.08456869373425491 1102 28-29 -0.021913812133256272 1102 30-31 0.16925787544729332 1102 32-33 0.23040375459049756 1102 34-35 0.17017189782130515 1102 36-37 0.25671892300384513 1102 38-39 0.2336795811788832 1102 40-41 0.11798667922355577 1102 42-43 0.12282180689133781 1102 44-45 0.4200620002340756 1102 46-47 0.3101498745249103 1102 48-49 0.4859127025855017 1102 50-51 0.3516975996128835 1102 52-53 0.21864286450634296 1102 54-55 0.3107984830745849 1102 56-57 0.32590111664186594 1102 58-59 0.4165033448545685 1102 60-61 0.44664983902877253 1102 62-63 0.3126170087868232 1102 64-65 0.3792596628733236 1102 66-67 0.43961340532904813 1102 68-69 0.10605099428430975 1102 70-71 0.16724977385681683 1102 72-73 0.2864938861110744 1102 74-75 0.30676305544977467 1102 76-77 0.12327641873203987 1102 78-79 0.18148584330367967 1102 80-81 0.45993429244076367 1102 82-83 0.3806156147579216 1102 84-85 0.40307241576851993 1102 86-87 0.4115492628462327 1102 88-89 0.26412049429835704 1102 90-91 0.37659683155351686 1102 92-93 0.36633173971455335 1102 94-95 0.35552686697953106 1102 96-97 0.5970680866795455 1102 98-99 0.3535259787612084 1102 100-101 0.9085029526456303 1103 1 0.005506701534393699 1103 2 0.06345763932615256 1103 3 -0.04953669856278964 1103 4 -0.035035688277091026 1103 5 -0.17115142821395324 1103 6 -0.06966751650054448 1103 7 0.04389871977149795 1103 8 -0.044182242394064986 1103 9 -0.049183308844973794 1103 10-11 -0.07538401939147121 1103 12-13 0.012042786115060267 1103 14-15 -0.07439129843724857 1103 16-17 -0.004894751839891853 1103 18-19 -0.1748417513380005 1103 20-21 -0.13843231621293484 1103 22-23 -0.18443147726338793 1103 24-25 -0.04913295645771143 1103 26-27 -0.048725168327621304 1103 28-29 -0.0013310777155766118 1103 30-31 -0.05050423946045157 1103 32-33 -0.00945460711252366 1103 34-35 -0.14549132245356589 1103 36-37 -0.09485927226366186 1103 38-39 0.08440143214345852 1103 40-41 0.0664000781223919 1103 42-43 -0.2077302462335311 1103 44-45 -0.10334719574792928 1103 46-47 -0.05092901422387541 1103 48-49 0.030338584147308723 1103 50-51 0.08881881173410022 1103 52-53 -0.09584458978693533 1103 54-55 -0.10842355702123996 1103 56-57 -0.06531583792460793 1103 58-59 0.1426801615653659 1103 60-61 0.15134975076752255 1103 62-63 -0.15580962930000908 1103 64-65 -0.2954513865950119 1103 66-67 9.623314837980956E-4 1103 68-69 -0.1953107507667582 1103 70-71 -0.1376571315355264 1103 72-73 -0.16263821156471536 1103 74-75 -0.31411283241640575 1103 76-77 -0.475293538818498 1103 78-79 -0.33418473207563437 1103 80-81 -0.10327127222110732 1103 82-83 -0.03368375900747367 1103 84-85 -0.2296966759811525 1103 86-87 -0.4083998818601948 1103 88-89 -0.46967831207326327 1103 90-91 -0.6285937707244607 1103 92-93 -0.3878638220052828 1103 94-95 -0.2779556699010648 1103 96-97 -0.3099140088947827 1103 98-99 -0.13035163230086866 1103 100-101 0.3113693419197894 1104 1 0.08629981858906888 1104 2 0.19788351727911646 1104 3 0.05254688496069093 1104 4 0.030470667982235966 1104 5 -0.045953706516890236 1104 6 0.01386968605911676 1104 7 0.09497696781144072 1104 8 0.0685534367220626 1104 9 0.029285184352708882 1104 10-11 0.06568633693648707 1104 12-13 0.06780673814718341 1104 14-15 0.1484629279176346 1104 16-17 0.17589922300956573 1104 18-19 -0.009246184421428438 1104 20-21 0.06126874230582047 1104 22-23 -0.029596516979289333 1104 24-25 0.04571898816305975 1104 26-27 0.10283816266107948 1104 28-29 0.0868866820675791 1104 30-31 -0.04764349386523037 1104 32-33 0.15631680773419276 1104 34-35 0.1844701625607641 1104 36-37 0.09002895707634906 1104 38-39 0.1314287443292912 1104 40-41 0.15996232828965873 1104 42-43 0.25339578097571547 1104 44-45 0.2854444287438227 1104 46-47 0.09688618246846659 1104 48-49 0.12149943782467432 1104 50-51 -0.08269253689085332 1104 52-53 -0.060370486611375895 1104 54-55 -0.0536876720343642 1104 56-57 0.08921717859652034 1104 58-59 -0.05005131435633814 1104 60-61 0.08329102613680561 1104 62-63 -0.0228106929655425 1104 64-65 -0.125853349229466 1104 66-67 -0.2426458107042535 1104 68-69 0.17309066287904074 1104 70-71 -0.0930878302951541 1104 72-73 -0.11252412485881891 1104 74-75 0.08481921308102969 1104 76-77 0.07701092686113142 1104 78-79 0.07664921601062602 1104 80-81 0.2839853285097327 1104 82-83 0.1352850858860748 1104 84-85 0.08901740386238544 1104 86-87 0.24947896320491125 1104 88-89 0.3576403072202545 1104 90-91 0.3231226766326749 1104 92-93 0.38564533066503515 1104 94-95 0.5369064824776011 1104 96-97 0.7192179885661467 1104 98-99 0.6637879137541312 1104 100-101 0.9240698514498895 1105 1 0.057208448063725825 1105 2 0.1255745864184732 1105 3 0.10206268727305456 1105 4 -0.019796740033221738 1105 5 -0.041546796392573526 1105 6 -0.0701665248813299 1105 7 -0.016769183753545747 1105 8 0.03721050279177973 1105 9 -0.07732290324072721 1105 10-11 -0.1037475278042379 1105 12-13 -0.10493149576069527 1105 14-15 0.029523998088876624 1105 16-17 0.1099059646849625 1105 18-19 0.12345010458932393 1105 20-21 0.12134792817322904 1105 22-23 0.08119932051027234 1105 24-25 0.0017547026811826072 1105 26-27 0.13987600882035167 1105 28-29 0.24965254457346475 1105 30-31 0.1692193866671161 1105 32-33 0.119798159824974 1105 34-35 0.21421854895581305 1105 36-37 -0.00786547788070635 1105 38-39 0.17334807663197438 1105 40-41 0.15472456152076575 1105 42-43 0.08922292374919749 1105 44-45 0.20771575607856363 1105 46-47 0.16058976256583435 1105 48-49 0.11794174453371653 1105 50-51 0.18866687007969318 1105 52-53 0.15551488817352777 1105 54-55 0.286640738289357 1105 56-57 0.23099757772379093 1105 58-59 0.13609868885088616 1105 60-61 0.18169489823088725 1105 62-63 0.3517794564910517 1105 64-65 0.1294606471840254 1105 66-67 0.1839752545213571 1105 68-69 0.1936598845092945 1105 70-71 0.2196040801591863 1105 72-73 0.275267232744671 1105 74-75 -0.021591560584791836 1105 76-77 -0.14954822501900011 1105 78-79 0.20443403831653484 1105 80-81 0.3114142885235651 1105 82-83 0.276307604309018 1105 84-85 0.14486189352815515 1105 86-87 0.10296535388927808 1105 88-89 0.17253427829203716 1105 90-91 0.32674497919557055 1105 92-93 0.4898103511122436 1105 94-95 0.359351604105715 1105 96-97 0.44452748557134214 1105 98-99 0.5213277228267614 1105 100-101 0.7229105101112019 1106 1 -0.024768180534213258 1106 2 0.16128440150269086 1106 3 0.12770885110428765 1106 4 0.008935736411821438 1106 5 -0.1264530025738182 1106 6 -0.14975215532957975 1106 7 0.016451279319575463 1106 8 -0.0720732601953955 1106 9 -0.06884921842119951 1106 10-11 -0.09713678083032562 1106 12-13 -0.04282734375506436 1106 14-15 0.17814010140356373 1106 16-17 0.06713755068568616 1106 18-19 -0.10893508700083743 1106 20-21 -0.10309900229217561 1106 22-23 -0.05689134176360966 1106 24-25 -0.2149838096404011 1106 26-27 -0.14343281494110016 1106 28-29 -0.09241245523192276 1106 30-31 0.10359763773559649 1106 32-33 0.07202579589254299 1106 34-35 0.05528081273561014 1106 36-37 -0.06257810052061075 1106 38-39 0.2610505304293227 1106 40-41 0.3883489065734622 1106 42-43 0.26514116486658423 1106 44-45 0.27015415342606275 1106 46-47 0.18343415623795067 1106 48-49 -0.06444865402558264 1106 50-51 0.1517723380284295 1106 52-53 0.16044993780804617 1106 54-55 0.040529134532349076 1106 56-57 0.07333896765845083 1106 58-59 0.04268355876606478 1106 60-61 0.043172688666686554 1106 62-63 0.13615307914251673 1106 64-65 0.39377560464992456 1106 66-67 0.2512442991424564 1106 68-69 0.2773369304010984 1106 70-71 0.34367379463844827 1106 72-73 0.2435446937213257 1106 74-75 0.1282405541073821 1106 76-77 -0.055143770798775193 1106 78-79 -0.035848389793105895 1106 80-81 -0.0427069486523024 1106 82-83 0.14371660594894564 1106 84-85 0.1975969198102021 1106 86-87 0.3026720805570591 1106 88-89 0.12452654883563596 1106 90-91 0.49077318518957824 1106 92-93 0.29316863582090136 1106 94-95 0.7104501694016356 1106 96-97 0.7211708628851561 1106 98-99 0.963017031919815 1106 100-101 1.0446386137540813 1107 1 0.016290426479969256 1107 2 0.11195812727751075 1107 3 -0.0030574552627342655 1107 4 -0.0032006158350839087 1107 5 -0.12541724909826968 1107 6 -0.04954327657861768 1107 7 -0.06448653659063552 1107 8 -0.0990298514324266 1107 9 -0.10351293875264389 1107 10-11 -0.03204361701927638 1107 12-13 -0.04933023392847247 1107 14-15 0.17915479419843905 1107 16-17 0.06470963221536152 1107 18-19 -0.06807142628732521 1107 20-21 -0.14812565755367046 1107 22-23 -0.05182975817322699 1107 24-25 0.024664055778607974 1107 26-27 -0.02538607967061779 1107 28-29 -0.16688734226027435 1107 30-31 -0.13107720695538916 1107 32-33 -0.08613991602325655 1107 34-35 -0.16401001474393695 1107 36-37 -0.07424895772271611 1107 38-39 -0.19219097833624232 1107 40-41 -0.2562737076294255 1107 42-43 -0.5235764664872633 1107 44-45 -0.3195443656300796 1107 46-47 -0.21656973188771644 1107 48-49 -0.24451315371020854 1107 50-51 -0.33243053140830625 1107 52-53 -0.4374293056491112 1107 54-55 -0.35754149111027544 1107 56-57 -0.5752306341110796 1107 58-59 -0.4608405951345844 1107 60-61 -0.41710794760347625 1107 62-63 -0.38871256451508174 1107 64-65 -0.3170347887419922 1107 66-67 -0.24754660177946874 1107 68-69 -0.11867892883146425 1107 70-71 -0.28659677570637854 1107 72-73 -0.24736627252109855 1107 74-75 -0.2621629037323743 1107 76-77 -0.4043876383605749 1107 78-79 -0.24638445431871148 1107 80-81 -0.049196878406831956 1107 82-83 -0.16524951983623737 1107 84-85 -0.22232767215261262 1107 86-87 -0.1369609883837306 1107 88-89 -0.06623731665249721 1107 90-91 -0.013064718851477863 1107 92-93 0.06918613670653073 1107 94-95 0.12176094296583528 1107 96-97 0.3057905177924063 1107 98-99 0.39310475235494025 1107 100-101 0.7588197960898952 1108 1 0.016104819013243343 1108 2 0.08124230114751185 1108 3 0.09921225890176544 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-0.7767359187281535 2316 94-95 -0.8924308383851418 2316 96-97 -0.6726023784317015 2316 98-99 -0.6243078987515673 2316 100-101 -1.1181088636163246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 15.0 5 23.0 6 67.0 7 156.0 8 362.0 9 690.0 10 1240.0 11 2063.0 12 3155.0 13 4437.0 14 5791.0 15 7963.0 16 10488.0 17 14041.0 18 12156.0 19 7442.0 20 6149.0 21 6444.0 22 6857.0 23 7675.0 24 8918.0 25 10785.0 26 13284.0 27 16295.0 28 20727.0 29 25652.0 30 32112.0 31 40884.0 32 51538.0 33 67675.0 34 91629.0 35 126556.0 36 183007.0 37 248522.0 38 162726.0 39 9883.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.5923933756881 19.503491451164425 14.670308911402977 21.2338062617445 2 15.467219510094141 21.762412058303667 39.74807302701655 23.02229540458564 3 20.73586573096664 25.817005102839484 30.03577205605255 23.411357110141324 4 12.776357832768209 17.134392687770934 38.2300178399572 31.859231639503662 5 13.025185376596294 38.89715729658682 33.91217708693092 14.16548023988597 6 30.333581522778903 37.047451116479806 17.772158776091985 14.846808584649304 7 27.96812029676648 33.84033976746998 20.665736855792627 17.525803079970913 8 26.281374905376282 34.124583614236705 19.688193113546802 19.905848366840207 9 26.66697587204198 17.545113616410855 20.687744534518913 35.10016597702825 10-11 24.75174091939096 26.951701496097424 27.200126220796943 21.096431363714668 12-13 27.101627583488035 24.339336435582638 27.622538778461386 20.93649720246794 14-15 23.3353776989664 25.761326040980354 27.275923515602123 23.627372744451122 16-17 21.332108090289754 29.12944935705799 27.830851857266087 21.707590695386163 18-19 21.040918856332624 28.119311189037067 30.371314230247453 20.468455724382856 20-21 22.48988333744681 26.384363606849014 30.065909456077204 21.05984359962697 22-23 21.790066477747782 26.48450612536975 29.952422422957163 21.7730049739253 24-25 22.134269337912865 26.573761917638834 30.323384557663708 20.968584186784593 26-27 22.120492784977248 26.126731282436953 30.929802823060903 20.822973109524888 28-29 21.22683293790629 27.17012418576471 30.75903356498497 20.844009311344028 30-31 21.490895688652014 26.8176942922573 30.38404995879559 21.307360060295096 32-33 21.49449772198153 26.921283508736476 30.252797519316154 21.331421249965835 34-35 22.19577660721304 26.411963228676893 30.20861849110349 21.183641673006573 36-37 22.355299423703332 26.227648572925442 30.51651339480774 20.900538608563483 38-39 22.00503275098319 26.483361108994348 30.701479012533838 20.810127127488627 40-41 21.814862882713808 26.861090428417494 30.38631866734802 20.937728021520677 42-43 21.50737075402081 27.316182530000237 29.59938115316542 21.577065562813534 44-45 21.978801513171923 26.934294629564832 29.249936329999027 21.83696752726422 46-47 22.387607890751593 27.355624233123116 28.709510039435536 21.547257836689752 48-49 22.62477073756144 27.296900086093768 28.564329518400832 21.513999657943963 50-51 22.12642428684718 27.713376442404254 28.950909079615123 21.209290191133444 52-53 22.32618427427871 28.07277711748945 28.243310957151195 21.35772765108064 54-55 21.784843748835293 27.51860842831148 28.517214504428175 22.17933331842506 56-57 22.417541552233068 27.847698683778976 28.004937899413285 21.72982186457467 58-59 22.871959794666566 27.378826604560867 27.922721678446784 21.826491922325786 60-61 22.257229586744685 27.413126933445973 28.942780352254704 21.386863127554633 62-63 22.485005427159486 27.318842410280002 28.71035699155218 21.485795171008327 64-65 22.16129716262242 28.00303484103529 28.416432537736725 21.419235458605563 66-67 22.099228327191923 27.694870781868463 28.149078539677337 22.05682235126228 68-69 21.938953413733785 27.96439045762991 28.16834565623051 21.928310472405794 70-71 22.441244213868824 27.85927433280907 27.815461652309526 21.884019801012577 72-73 22.415337463708067 27.33476534013474 28.145062537451448 22.104834658705734 74-75 22.001714512896122 28.530322858515415 27.7356005764739 21.732362052114564 76-77 22.37941264653606 27.682166870137202 27.78047875485969 22.157941728467048 78-79 22.24724908157776 27.725273828765758 28.007954482865436 22.019522606791046 80-81 22.437296974054092 28.11369949923718 27.616705235729583 21.832298290979146 82-83 22.33805796277998 28.006702220793944 27.779056968048494 21.876182848377574 84-85 22.111520869008842 27.986731298440183 27.693072238637846 22.208675593913128 86-87 22.194540463245218 27.98574757521181 27.524953959980074 22.294758001562897 88-89 22.353252514677234 27.74241455398439 27.364094782458885 22.540238148879492 90-91 22.14335294780298 27.494798189637464 27.766816425793028 22.59503243676653 92-93 22.23486646110116 27.918272388378014 27.756478584993715 22.09038256552711 94-95 21.93996461783619 27.8973287791497 27.628980907545873 22.533725695468235 96-97 21.494323558216916 27.678591024948183 28.078167802369137 22.748917614465764 98-99 21.843836175784002 27.988010397724988 27.19600589541234 22.972147531078672 100-101 21.658239223432847 28.171845299474473 27.166971048523024 23.002944428569656 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 337.0 1 256.0 2 244.5 3 297.5 4 235.5 5 219.5 6 319.5 7 602.0 8 945.0 9 1199.5 10 1251.5 11 1157.0 12 1192.0 13 1333.0 14 1488.0 15 1630.0 16 1756.0 17 1880.5 18 2033.0 19 2155.5 20 2362.0 21 2735.5 22 3177.5 23 3662.5 24 4316.0 25 5152.0 26 6348.0 27 8036.5 28 9842.0 29 12133.0 30 15090.5 31 18064.5 32 21096.0 33 23627.0 34 26332.5 35 28897.0 36 31482.5 37 34129.0 38 35730.5 39 36731.5 40 37524.5 41 38988.0 42 40125.0 43 41060.5 44 42587.0 45 44026.5 46 45076.0 47 50917.0 48 68631.0 49 66321.5 50 47491.5 51 40542.5 52 36881.0 53 34789.0 54 32191.5 55 29424.0 56 26529.0 57 23666.5 58 21633.0 59 19208.0 60 15905.5 61 12915.5 62 10497.5 63 7946.5 64 5635.5 65 4251.5 66 3219.5 67 2448.0 68 1770.0 69 1219.0 70 968.0 71 753.0 72 609.0 73 567.5 74 486.0 75 404.0 76 277.5 77 170.0 78 128.5 79 96.5 80 64.5 81 54.5 82 42.5 83 26.0 84 15.5 85 9.5 86 5.5 87 2.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03859488129175246 2 0.006625730693863083 3 0.003892616782644561 4 9.938596040794624E-4 5 5.797514357130197E-4 6 2.484649010198656E-4 7 0.0 8 0.0 9 0.0014907894061191936 10-11 4.969298020397312E-4 12-13 8.696271535695295E-4 14-15 0.0025260598270353003 16-17 0.0028573463617284542 18-19 0.0 20-21 0.0 22-23 0.0014493785892825495 24-25 0.0010352704209161067 26-27 0.005963157624476774 28-29 0.005756103540293552 30-31 0.001573611039792482 32-33 4.141081683664427E-4 34-35 0.009483077055591536 36-37 0.011305152996403885 38-39 0.010104239308141201 40-41 0.001407967772445905 42-43 5.797514357130197E-4 44-45 0.0013665569556092607 46-47 4.141081683664427E-5 48-49 7.86805519896241E-4 50-51 9.524487872428182E-4 52-53 0.0024018273765253674 54-55 0.0025674706438719446 56-57 0.0014907894061191936 58-59 9.938596040794624E-4 60-61 0.004058260049991138 62-63 0.005010708837233957 64-65 0.00844780663467543 66-67 0.0030644004459116757 68-69 0.0033956869806048297 70-71 8.282163367328854E-5 72-73 0.0014493785892825495 74-75 0.0061288008918233515 76-77 0.002650292277545233 78-79 0.00368556269846134 80-81 0.0046380114857041575 82-83 0.0043895465846842925 84-85 0.005093530470907244 86-87 0.0033542761637681854 88-89 0.00844780663467543 90-91 0.011760671981606971 92-93 0.0010766812377527508 94-95 0.0021533624755055015 96-97 9.524487872428182E-4 98-99 0.0028573463617284542 100-101 0.004058260049991138 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1207414.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.260481072529124 #Duplication Level Percentage of deduplicated Percentage of total 1 80.62091054808975 49.38875764681316 2 11.903523449939112 14.58431146002803 3 3.412723827567869 6.2719531033347184 4 1.4064415900242309 3.446371536211886 5 0.7178912578445832 2.198918190666111 6 0.44939941133476285 1.651825447924738 7 0.25757588431675227 1.104545581014845 8 0.20537931428810485 1.0065308476508366 9 0.1411150098929892 0.7780296053339303 >10 0.8017426679737986 8.942461445905652 >50 0.050249720395967444 2.0910413380330235 >100 0.028056511010242167 3.214894923640049 >500 0.002023300265597979 0.8430140283115151 >1k 0.002697733687463972 3.407422708434977 >5k 2.697733687463972E-4 1.0699221366964808 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7419 0.6144537002221276 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5529 0.4579208125796123 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4904 0.406157291533807 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3899 0.322921549692152 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3716 0.3077651907299402 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2896 0.2398514511178436 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2704 0.2239496974525722 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2427 0.2010081049250713 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2116 0.17525057685267853 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1953 0.1617506505639325 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1848 0.1530543790282372 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1690 0.13996856090785761 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1608 0.13317718694664796 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1562 0.12936739179767667 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1475 0.12216190966810059 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1465 0.1213336933313677 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1318 0.10915891318139428 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1279 0.10592886946813604 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.6564326734657707E-4 0.0 22-23 0.0 0.0 0.0 4.969298020397312E-4 0.0 24-25 0.0 0.0 0.0 7.453947030595968E-4 0.0 26-27 0.0 0.0 0.0 0.0010352704209161067 0.0 28-29 0.0 0.0 0.0 0.0019877192081589248 0.0 30-31 0.0 0.0 0.0 0.003230043713258253 0.0 32-33 0.0 0.0 0.0 0.0053005845550904655 0.0 34-35 0.0 0.0 0.0 0.007536768664269256 0.0 36-37 0.0 0.0 0.0 0.011015277278547375 0.0 38-39 0.0 0.0 0.0 0.014203910174968984 0.0 40-41 0.0 0.0 0.0 0.018303581041796767 0.0 42-43 0.0 0.0 0.0 0.02426673866627354 4.141081683664427E-5 44-45 8.282163367328854E-5 0.0 0.0 0.03374981572186508 8.282163367328854E-5 46-47 8.282163367328854E-5 0.0 0.0 0.05776808948711875 8.282163367328854E-5 48-49 8.282163367328854E-5 0.0 0.0 0.10199484186865482 8.282163367328854E-5 50-51 8.282163367328854E-5 0.0 0.0 0.13272166796144486 8.282163367328854E-5 52-53 8.282163367328854E-5 0.0 0.0 0.15872766093485746 1.242324505099328E-4 54-55 8.282163367328854E-5 0.0 0.0 0.1991446181674223 1.6564326734657707E-4 56-57 8.282163367328854E-5 0.0 0.0 0.2290846387403161 1.6564326734657707E-4 58-59 8.282163367328854E-5 0.0 0.0 0.27136508273052984 1.6564326734657707E-4 60-61 8.282163367328854E-5 0.0 0.0 0.3385748384564035 1.6564326734657707E-4 62-63 8.282163367328854E-5 0.0 0.0 0.3898414297001691 1.6564326734657707E-4 64-65 8.282163367328854E-5 0.0 0.0 0.4423917562658707 1.6564326734657707E-4 66-67 8.282163367328854E-5 0.0 0.0 0.5520061884324681 1.6564326734657707E-4 68-69 8.282163367328854E-5 0.0 0.0 0.6778950716158667 1.6564326734657707E-4 70-71 8.282163367328854E-5 0.0 0.0 0.7755417777166738 1.6564326734657707E-4 72-73 8.282163367328854E-5 0.0 0.0 0.8856945505021476 1.6564326734657707E-4 74-75 8.282163367328854E-5 0.0 0.0 0.9763428285575618 1.6564326734657707E-4 76-77 8.282163367328854E-5 0.0 0.0 1.0399084324018109 1.6564326734657707E-4 78-79 8.282163367328854E-5 0.0 0.0 1.1310950510761015 1.6564326734657707E-4 80-81 8.282163367328854E-5 0.0 0.0 1.2815405486436302 2.0705408418322135E-4 82-83 8.282163367328854E-5 0.0 0.0 1.4585717906202844 2.484649010198656E-4 84-85 8.282163367328854E-5 0.0 0.0 1.6594556630948456 2.484649010198656E-4 86-87 8.282163367328854E-5 0.0 0.0 2.0188187316032447 2.484649010198656E-4 88-89 8.282163367328854E-5 0.0 0.0 2.4846490101986562 2.484649010198656E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1225 0.0 28.692528 2 GTACCTG 1335 0.0 27.75725 1 GTACATG 7465 0.0 23.101486 1 GAGTACT 5990 0.0 22.79923 12-13 TACATGG 7660 0.0 22.446724 2 GTAAGGT 490 0.0 22.294868 4 ACATGGG 7470 0.0 21.936718 3 GTACTTT 6360 0.0 21.286139 14-15 TAAGGTG 605 0.0 21.198227 5 AGTACTT 6245 0.0 20.651258 12-13 CCTATAC 255 1.19271135E-8 20.489231 3 GTGTAGC 655 0.0 20.308594 1 CATGGGA 5100 0.0 19.185371 4 CTGATCG 125 0.006028628 19.001467 9 GGTAAGG 525 0.0 18.999105 3 ACCTGGG 1800 0.0 18.73523 3 TACACGG 255 2.6764246E-7 18.626574 2 ACTTTTT 7365 0.0 18.573078 16-17 CATGGGG 2305 0.0 18.3397 4 GTATAGA 415 1.8189894E-12 18.316183 1 >>END_MODULE