FastQCFastQC Report
Thu 26 May 2016
SRR936536_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936536_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265914
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58800.464486529100713No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43350.34244032375027056No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40490.3198479517565964No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA36950.29188396684134943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34820.27505817930759907No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32090.2534927333136374No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26820.21186273317144766No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26440.2088609494799805No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT24520.19369404240730412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20930.1653350859537062No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17460.13792406119215048No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT17180.1357122205773852No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG14190.1120929225839986No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA13960.11027605350758425No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC13630.10766924135446801No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT12970.1024556170482355No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC12820.10127070243318266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG11350.030.1473672
GTACCTG13150.027.1059991
GTACATG75400.026.4102551
TACATGG75900.025.9221692
ACATGGG74800.024.9730953
CATGGGA51750.021.6770534
CATGGGG22600.021.6634374
GTATAGA3950.021.657351
CATACCG1100.002869110821.6018092
GAGTACT47950.021.26446512-13
GTGTAAG5550.019.69541
GTACTTT52300.019.53546714-15
AGTACTT50050.019.18505712-13
ACACCGC1759.857393E-519.0201178
GTGTACG1250.00601254619.0103421
ATGGGAG17250.018.7392735
ATGGGAT20250.018.545355
ACCTGGG18400.017.824313
GTGTAGC8300.017.750621
ATGGGGG13550.017.5413765
Produced by FastQC (version 0.11.5)